4,351 research outputs found
Chirp mitigation of plasma-accelerated beams using a modulated plasma density
Plasma-based accelerators offer the possibility to drive future compact light
sources and high-energy physics applications. Achieving good beam quality,
especially a small beam energy spread, is still one of the major challenges.
For stable transport, the beam is located in the focusing region of the
wakefield which covers only the slope of the accelerating field. This, however,
imprints a longitudinal energy correlation (chirp) along the bunch. Here, we
propose an alternating focusing scheme in the plasma to mitigate the
development of this chirp and thus maintain a small energy spread
Measurement of intracellular concentration of fluorescently-labeled targets in living cells
Estimations of intracellular concentrations of fluorescently-labeled molecules within living cells are very important for guidance of biological experiments and interpretation of their results. Here we propose a simple and universal approach for such estimations. The approach is based upon common knowledge that the dye fluorescence is directly proportional to its quantum yield and the number of its molecules and that a coefficient of proportionality is determined by spectral properties of the dye and optical equipment used to record fluorescent signals. If two fluorescent dyes are present in the same volume, then a ratio of their concentrations is equal to a ratio of their fluorescence multiplied by some dye- and equipment-dependent coefficient. Thus, if the coefficient and concentration of one dye is known then the concentration of another dye can be determined. Here we have demonstrated how to calculate this coefficient (called a ratio factor) and how to use it for concentration measurements of fluorescently tagged molecules. As an example of how this approach can be used, we estimated a concentration of exogenously expressed neuronal Ca2+ sensor protein, hippocalcin, tagged by a fluorescent protein in a dendritic tree of rat hippocampal neurons loaded via a patch pipette with Alexa Fluor dye of known concentration. The new approach should allow performing a fast, inexpensive and reliable quantitative analysis of fluorescently-labeled targets in different parts of living cells
The FLASHForward Facility at DESY
The FLASHForward project at DESY is a pioneering plasma-wakefield
acceleration experiment that aims to produce, in a few centimetres of ionised
hydrogen, beams with energy of order GeV that are of quality sufficient to be
used in a free-electron laser. The plasma wave will be driven by high-current
density electron beams from the FLASH linear accelerator and will explore both
external and internal witness-beam injection techniques. The plasma is created
by ionising a gas in a gas cell with a multi-TW laser system, which can also be
used to provide optical diagnostics of the plasma and electron beams due to the
<30 fs synchronisation between the laser and the driving electron beam. The
operation parameters of the experiment are discussed, as well as the scientific
program.Comment: 19 pages, 9 figure
Measurement of the Pion Form Factor in the Energy Range 1.04-1.38 GeV with the CMD-2 Detector
The cross section for the process is measured in the
c.m. energy range 1.04-1.38 GeV from 995 000 selected collinear events
including 860000 events, 82000 events, and 33000
events. The systematic and statistical errors of measuring the
pion form factor are equal to 1.2-4.2 and 5-13%, respectively.Comment: 5 pages, 2 figure
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