Fungos associados a clones de camucamuzeiro, muricizeiro e bacurizeiro no município de Tomé-Açu.

Diversos fatores são responsáveis pela redução da produtividade e morte de espécies frutíferas da Amazônia, dentre os quais, destaca-se a incidência de doenças, principalmente pelas condições edafoclimáticas da região. O objetivo do trabalho foi isolar e identificar fungos associados aos clones de camucamuzeiro, muricizeiro e bacurizeiro em experimentos localizados no Campo Experimental de Tomé-Açu-PA. Plantas apresentando sintomas de doenças foram fotografadas, coletadas, acondicionadas em sacos plásticos e transportadas para o Laboratório de Fitopatologia da Embrapa Amazônia Oriental. Foram realizados exames microscópicos, bem como o isolamento em meio de cultura para a classificação dos patógenos. Do material sintomático de plantas de bacurizeiro foram isolados os seguintes fungos: Pestalotiopsis sp., Lasiodiplodia sp., Curvularia sp., Phomopsis sp., Guignardia sp. e Nigrospora sp. De plantas sintomáticas de muricizeiro foram isolados, Pestalotiopsis sp., Lasiodiplodia sp., Calonectria sp., Rhizoctonia sp. e Phomopsis sp. e de plantas de camucamuzeiro isolou-se os fungos Lasiodiplodia sp., Phomopsis sp., Curvularia sp. e Guignardia sp. Todos os isolados fúngicos se encontram preservados em óleo mineral para posteriores testes de patogenicidade

Early rootstock selection under 'tahiti' acid lime crown in Capitão Poço, Pará State, Brazil.

Citrus represents one of the main fruit crops grown in the world, highlighting the ‘Tahiti’ acid lime tree. The objective was to compare rootstock varieties combined with this scion to identify those with the best agronomic performance and precocity of production, in addition to Phytophthora gummosis resistance. The experiment was carried out in Capitão Poço, northeast region of Pará, and the following seven rootstock genotypes were evaluated: ‘Sunki Tropical’, ‘BRS O S Passos’, ‘BRS Bravo’, ‘BRS Donadio’, ‘Citrandarin Indio’, ‘BRS Matta’, and ‘LVK x LCR-038’. A randomized block experimental design was used, with three replications and 10 plants per plot. The evaluated characteristics were: plant height (m); crown volume (m3); number of ripe fruit per plant; total yield of ripened fruit per plant (kg plant-1); cumulative fruit yield (fruit kg plant-1); and average productive efficiency (fruit kg m-3 of crown). The average productive efficiency was high. For early selection, the best rootstocks in terms of yield, stability and adaptability wereSunki Tropical, BRS O S Passos, and Cintradarin Indio. The fruit number had the greatest direct effect on fruit yield, and crown pruning was directly harmful. Further studies to understand the complex interaction of G x E for BRS Donadio and Sunki Tropical should be carried out

A Deep Insight into the Sialome of Rhodnius neglectus, a vector of chagas disease

Background Triatomines are hematophagous insects that act as vectors of Chagas disease. Rhodnius neglectus is one of these kissing bugs found, contributing to the transmission of this American trypanosomiasis. The saliva of hematophagous arthropods contains bioactive molecules responsible for counteracting host haemostatic, inflammatory, and immuneresponses. Methods/Principal Findings Next generation sequencing and mass spectrometry-based protein identification were performed to investigate the content of triatomine R. neglectus saliva.We deposited 4,230 coding DNA sequences (CDS) in GenBank. A set of 636 CDS of proteins of putative secretory nature was extracted from the assembled reads, 73 of them confirmed by proteomic analysis. The sialome of R. neglectus was characterized and serine protease transcripts detected. The presence of ubiquitous protein families was revealed, including lipocalins, serine protease inhibitors, and antigen-5. Metalloproteases, disintegrins, and odorant binding protein families were less abundant. Conclusions/Significance The data presented improve our understanding of hematophagous arthropod sialomes, and aid in understanding hematophagy and the complex interplay among vectors and their vertebrate hosts

Evidence of multiple paternity and cooperative parental care in the so called monogamous silver arowana Osteoglossum bicirrhosum (Osteoglossiformes: Osteoglossidae)

Monogamy is rare in fishes and is usually associated with elaborate parental care. When parental care is present in fishes, it is usually the male that is responsible, and it is believed that there is a relationship between the high energetic investment and the certainty of paternity (except in the case of sneaker males). Osteoglossum bicirrhosum is considered a monogamous fish, and has particular behavioral traits that permit the study of mating systems and parental care, such as male mouthbrooding. We investigated the genetic relationships of males with the broods found in their oral cavities in Osteoglossum samples collected in a natural environment in the lower Purus river basin, Amazonas, Brazil. Fourteen broods were analyzed for parentage (268 young and 14 adult males) using eight microsatellite loci. The results indicate that eleven broods show a monogamous system. In one brood, however, approximately 50% of the young were genetically compatible with being offspring of another male, and in another two broods, none of the subsampled young were compatible with the genotypes of the brooding male. The result of this first brood may be explained by the extra-parental contribution of a sneaker male, whereas cooperative parental care may explain the result in the other two broods