Uncertainties of size measurements in electron microscopy characterization of nanomaterials in foods

Electron microscopy is a recognized standard tool for nanomaterial characterization, and recommended by the European Food Safety Authority for the size measurement of nanomaterials in food. Despite this, little data have been published assessing the reliability of the method, especially for size measurement of nanomaterials characterized by a broad size distribution and/or added to food matrices. This study is a thorough investigation of the measurement uncertainty when applying electron microscopy for size measurement of engineered nanomaterials in foods. Our results show that the number of measured particles was only a minor source of measurement uncertainty for nanomaterials in food, compared to the combined influence of sampling, sample preparation prior to imaging and the image analysis. The main conclusion is that to improve the measurement reliability, care should be taken to consider replications and matrix removal prior to sample preparation

Speaker-independent Speech Inversion for Estimation of Nasalance

The velopharyngeal (VP) valve regulates the opening between the nasal and oral cavities. This valve opens and closes through a coordinated motion of the velum and pharyngeal walls. Nasalance is an objective measure derived from the oral and nasal acoustic signals that correlate with nasality. In this work, we evaluate the degree to which the nasalance measure reflects fine-grained patterns of VP movement by comparison with simultaneously collected direct measures of VP opening using high-speed nasopharyngoscopy (HSN). We show that nasalance is significantly correlated with the HSN signal, and that both match expected patterns of nasality. We then train a temporal convolution-based speech inversion system in a speaker-independent fashion to estimate VP movement for nasality, using nasalance as the ground truth. In further experiments, we also show the importance of incorporating source features (from glottal activity) to improve nasality prediction.Comment: Interspeech 202

Spinodal decomposition in alkali feldspar studied by atom probe tomography

We used atom probe tomography to complement electron microscopy for the investigation of spinodal decomposition in alkali feldspar. To this end, gem-quality alkali feldspar of intermediate composition with a mole fraction of aK=0.43 of the K end-member was prepared from Madagascar orthoclase by ion-exchange with (NaK)Cl molten salt. During subsequent annealing at 550∘C and close to ambient pressure the ion-exchanged orthoclase unmixed producing a coherent lamellar intergrowth of Na-rich and K-rich lamellae. The chemical separation was completed, and equilibrium Na–K partitioning between the different lamellae was attained within four days, which was followed by microstructural coarsening. After annealing for 4 days, the wavelength of the lamellar microstructure was ≈17nm and it increased to ≈30nm after annealing for 16 days. The observed equilibrium compositions of the Na-rich and K-rich lamellae are in reasonable agreement with an earlier experimental determination of the coherent solvus. The excess energy associated with compositional gradients at the lamellar interfaces was quantified from the initial wavelength of the lamellar microstructure and the lamellar compositions as obtained from atom probe tomography using the Cahn–Hilliard theory. The capability of atom probe tomography to deliver quantitative chemical compositions at nm resolution opens new perspectives for studying the early stages of exsolution. In particular, it helps to shed light on the phase relations in nm scaled coherent intergrowth

The Clustering of Extragalactic Extremely Red Objects

We have measured the angular and spatial clustering of 671 K5 Extremely Red Objects (EROs) from a 0.98 square degree sub-region of the NOAO Deep Wide-Field Survey (NDWFS). Our study covers nearly 5 times the area and has twice the sample size of any previous ERO clustering study. The wide field of view and BwRIK passbands of the NDWFS allow us to place improved constraints on the clustering of z=1 EROs. We find the angular clustering of EROs is slightly weaker than in previous measurements, and w(1')=0.25+/-0.05 for K<18.40 EROs. We find no significant correlation of ERO spatial clustering with redshift, apparent color or absolute magnitude, although given the uncertainties, such correlations remain plausible. We find the spatial clustering of K5 EROs is well approximated by a power-law, with r_0=9.7+/-1.1 Mpc/h in comoving coordinates. This is comparable to the clustering of 4L* early-type galaxies at z<1, and is consistent with the brightest EROs being the progenitors of the most massive ellipticals. There is evidence of the angular clustering of EROs decreasing with increasing apparent magnitude, when NDWFS measurements of ERO clustering are combined with those from the literature. Unless the redshift distribution of K>20 EROs is very broad, the spatial clustering of EROs decreases from r_0=9.7+/-1.1 Mpc/h for K20 EROs.Comment: Accepted for publication in the ApJ. 29 pages with 10 figures. The NOAO Deep Wide-Field Survey Bootes data release is available online at http://www.noao.edu/noao/noaodeep

Finding rare objects and building pure samples: Probabilistic quasar classification from low resolution Gaia spectra

We develop and demonstrate a probabilistic method for classifying rare objects in surveys with the particular goal of building very pure samples. It works by modifying the output probabilities from a classifier so as to accommodate our expectation (priors) concerning the relative frequencies of different classes of objects. We demonstrate our method using the Discrete Source Classifier, a supervised classifier currently based on Support Vector Machines, which we are developing in preparation for the Gaia data analysis. DSC classifies objects using their very low resolution optical spectra. We look in detail at the problem of quasar classification, because identification of a pure quasar sample is necessary to define the Gaia astrometric reference frame. By varying a posterior probability threshold in DSC we can trade off sample completeness and contamination. We show, using our simulated data, that it is possible to achieve a pure sample of quasars (upper limit on contamination of 1 in 40,000) with a completeness of 65% at magnitudes of G=18.5, and 50% at G=20.0, even when quasars have a frequency of only 1 in every 2000 objects. The star sample completeness is simultaneously 99% with a contamination of 0.7%. Including parallax and proper motion in the classifier barely changes the results. We further show that not accounting for class priors in the target population leads to serious misclassifications and poor predictions for sample completeness and contamination. (Truncated)Comment: MNRAS accepte

Clinical outcomes after joint surgery in patients on turoctocog alfa pegol (N8-GP) prophylaxis: a post hoc analysis

Introduction Joint damage in haemophilia often requires surgical correction. However, the surgery effect on bleeding rates and other clinical joint outcomes can be unclear. Aim To investigate the effects of joint surgery on joint annualized bleeding rates (JABRs) and physical health outcomes in patients with haemophilia A undergoing N8-GP prophylaxis. Methods Patients in the pathfinder 2 trial received N8-GP prophylaxis, enrolling in the pathfinder 3 trial for indicated surgery. Patients returned to pathfinder two post-surgery, continuing N8-GP prophylaxis until end-of-trial. JABRs were calculated from bleeding across all joints for pre-surgery (immediately before surgery) and post-surgery (to pathfinder 2 study end) periods. Joint-health-related outcomes were derived from patient records. Results Data (41 joint surgeries; n = 30) were analysed statistically using datamining and descriptively. Pre-surgery mean JABR was higher in patients who later were operated than in 146 non-operated patients (p = .004). In operated patients, mean JABR decreased from 1.33 pre-surgery to .37 post-surgery (p = .011). In all but three patients, JABR improved or remained the same post-surgery. In the three patients whose JABR remained at one (all with multiple joint arthropathy), post-surgery bleeds were mostly at non-operated sites. Two of the three patients whose JABR increased post-surgery had undergone surgery for reasons unlikely to improve JABR. Mobility parameters often improved in patients whose JABR remained at zero. Conclusion Patients with haemophilia treated with N8-GP prophylaxis benefit from surgeries. However, this analysis could not differentiate the relative contributions of surgical interventions and prophylactic treatment to the improvement of JABR