564 research outputs found
Relevance of the light signaling machinery for cellulase expression in trichoderma reesei (hypocrea jecorina)
<p>Abstract</p> <p>Background</p> <p>In nature, light is one of the most important environmental cues that fungi perceive and interpret. It is known not only to influence growth and conidiation, but also cellulase gene expression. We therefore studied the relevance of the main components of the light perception machinery of <it>Trichoderma reesei </it>(<it>Hypocrea jecorina</it>), ENV1, BLR1 and BLR2, for production of plant cell wall degrading enzymes in fermentations aimed at efficient biosynthesis of enzyme mixtures for biofuel production.</p> <p>Findings</p> <p>Our results indicate that despite cultivation in mostly dark conditions, all three components show an influence on cellulase expression. While we found the performance of the enzyme mixture secreted by a deletion mutant in <it>env1 </it>to be enhanced, the higher cellulolytic activity observed for <it>Δblr2 </it>is mainly due to an increased secretion capacity of this strain. <it>Δblr1 </it>showed enhanced biomass accumulation, but due to its obviously lower secretion capacity still was the least efficient strain in this study.</p> <p>Conclusions</p> <p>We conclude that with respect to regulation of plant cell wall degrading enzymes, the blue light regulator proteins are unlikely to act as a complex. Their regulatory influence on cellulase biosynthesis involves an alteration of protein secretion, which may be due to adjustment of transcription or posttranscriptional regulation of upstream factors. In contrast, the regulatory function of ENV1 seems to involve adjustment of enzyme proportions to environmental conditions.</p
3D Spectrophotometry of Planetary Nebulae in the Bulge of M31
We introduce crowded field integral field (3D) spectrophotometry as a useful
technique for the study of resolved stellar populations in nearby galaxies. As
a methodological test, we present a pilot study with selected extragalactic
planetary nebulae (XPN) in the bulge of M31, demonstrating how 3D spectroscopy
is able to improve the limited accuracy of background subtraction which one
would normally obtain with classical slit spectroscopy. It is shown that due to
the absence of slit effects, 3D is a most suitable technique for
spectrophometry. We present spectra and line intensities for 5 XPN in M31,
obtained with the MPFS instrument at the Russian 6m BTA, INTEGRAL at the WHT,
and with PMAS at the Calar Alto 3.5m Telescope. Using 3D spectra of bright
standard stars, we demonstrate that the PSF is sampled with high accuracy,
providing a centroiding precision at the milli-arcsec level. Crowded field 3D
spectrophotometry and the use of PSF fitting techniques is suggested as the
method of choice for a number of similar observational problems, including
luminous stars in nearby galaxies, supernovae, QSO host galaxies,
gravitationally lensed QSOs, and others.Comment: (1) Astrophysikalisches Institut Potsdam, (2) University of Durham.
18 pages, 11 figures, accepted for publication in Ap
A magnetically-driven piston pump for ultra-clean applications
A magnetically driven piston pump for xenon gas recirculation is presented.
The pump is designed to satisfy extreme purity and containment requirements, as
is appropriate for the recirculation of isotopically enriched xenon through the
purification system and large liquid xenon TPC of EXO-200. The pump, using
sprung polymer gaskets, is capable of pumping more than 16 standard liters per
minute (SLPM) of xenon gas with 750 torr differential pressure.Comment: 6 pages, 5 figure
Modeling the time-resolved quasi-periodic oscillations in AGNs
Observation of the bright Seyfert 1 galaxy RE J1034+396 is believed to
demonstrate a drift of the central period of the Quasi Periodic Oscillation
(QPO) linearly correlated with the temporary X-ray luminosity. We show, using a
specific scenario of the oscillation mechanism in black hole accretion disc,
that modeling such correlated trends puts very strong constraints on the nature
of this oscillation and the characteristic features of the hot flow in Active
Galactic Nuclei (AGN). In our model, QPO oscillations are due to the
oscillations of the shock formed in the low angular momentum hot accretion
flow, and the variation of the shock location corresponds to the observed
changes in the QPO period and the X-ray flux. In this scenario, change in the
shock location caused by perturbation of the flow angular momentum is
compatible with the trends observed in RE J1034+396, whereas the perturbation
of the specific flow energy results in too strong flux response to the change
of the oscillation period. Using a complete general relativistic framework to
study the accretion flow in the Kerr metric, we discuss the role of the black
hole spin in the period drift. Future missions are expected to bring more
active galaxies with time-resolved quasi-periodic oscillations so similar
quantitative study for other QPO scenarios will be necessary.Comment: Final version accepted for publication in MNRAS. Several
modifications made. Eight pages. Six colour figure
L\'evy-stable two-pion Bose-Einstein correlations in GeV AuAu collisions
We present a detailed measurement of charged two-pion correlation functions
in 0%-30% centrality GeV AuAu collisions by the
PHENIX experiment at the Relativistic Heavy Ion Collider. The data are well
described by Bose-Einstein correlation functions stemming from L\'evy-stable
source distributions. Using a fine transverse momentum binning, we extract the
correlation strength parameter , the L\'evy index of stability
and the L\'evy length scale parameter as a function of average
transverse mass of the pair . We find that the positively and the
negatively charged pion pairs yield consistent results, and their correlation
functions are represented, within uncertainties, by the same L\'evy-stable
source functions. The measurements indicate a decrease of the
strength of the correlations at low . The L\'evy length scale parameter
decreases with increasing , following a hydrodynamically
predicted type of scaling behavior. The values of the L\'evy index of stability
are found to be significantly lower than the Gaussian case of
, but also significantly larger than the conjectured value that may
characterize the critical point of a second-order quark-hadron phase
transition.Comment: 448 authors, 25 pages, 11 figures, 4 tables, 2010 data. v2 is version
accepted for publication in Phys. Rev. C. Plain text data tables for the
points plotted in figures for this and previous PHENIX publications are (or
will be) publicly available at http://www.phenix.bnl.gov/papers.htm
Measurements of double-helicity asymmetries in inclusive production in longitudinally polarized collisions at GeV
We report the double helicity asymmetry, , in inclusive
production at forward rapidity as a function of transverse momentum
and rapidity . The data analyzed were taken during
GeV longitudinally polarized collisions at the Relativistic Heavy Ion
Collider (RHIC) in the 2013 run using the PHENIX detector. At this collision
energy, particles are predominantly produced through gluon-gluon
scatterings, thus is sensitive to the gluon polarization
inside the proton. We measured by detecting the decay
daughter muon pairs within the PHENIX muon spectrometers in the
rapidity range . In this kinematic range, we measured the
to be ~(stat)~~(syst). The
can be expressed to be proportional to the product of the
gluon polarization distributions at two distinct ranges of Bjorken : one at
moderate range where recent RHIC data of jet and
double helicity spin asymmetries have shown evidence for significant gluon
polarization, and the other one covering the poorly known small- region . Thus our new results could be used to further
constrain the gluon polarization for .Comment: 335 authors, 10 pages, 4 figures, 3 tables, 2013 data. Version
accepted for publication by Phys. Rev. D. Plain text data tables for the
points plotted in figures for this and previous PHENIX publications are (or
will be) publicly available at http://www.phenix.bnl.gov/papers.htm
A False Start in the Race Against Doping in Sport: Concerns With Cycling’s Biological Passport
Professional cycling has suffered from a number of doping scandals. The sport’s governing bodies have responded by implementing an aggressive new antidoping program known as the biological passport. Cycling’s biological passport marks a departure from traditional antidoping efforts, which have focused on directly detecting prohibited substances in a cyclist’s system. Instead, the biological passport tracks biological variables in a cyclist’s blood and urine over time, monitoring for fluctuations that are thought to indirectly reveal the effects of doping. Although this method of indirect detection is promising, it also raises serious legal and scientific concerns. Since its introduction, the cycling community has debated the reliability of indirect biological-passport evidence and the clarity, consistency, and transparency of its use in proving doping violations. Such uncertainty undermines the legitimacy of finding cyclists guilty of doping based on this indirect evidence alone. Antidoping authorities should address these important concerns before continuing to pursue doping sanctions against cyclists solely on the basis of their biological passports
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