238 research outputs found
Continuous culture of rotifers for larval rearing of marine prawns
After continued harvesting, when the rotiter population declines due to their resorting to sexual reproduction, the culture is rejuvenated in the same pool by renewing 1/3 volume of water and by refertilising. This continous culture with frequent harvesting can be maintained for over 2 months
An unusual bumber catch of white prawn, Penaeus indicus from Kovalam bay near Madras
In the history of the fishing village, namely, Kovalam (lat. 12┬░47'N long. 80┬░15'E), formerly known as Covelong, situated 35 km south of Madras City, heavy landings of the Indian white prawn, Penaeus indicus caught with gill net have occurred for the firsi time during 16th-20th December, 1984. Recent mark-recapture experiments on P. indicus have also established the fact that a longer migration of tagged white prawns took place from Cochin, (place of release) to Ovari - Manappad fishing villages on the southeast coast (Tinnaveli coast), covering a distance of 330-380 km in 68 to 103 days at a rate of 3.5 to 5.5 km/day. The southward migration of white prawns during the present study was in conjunction with the prevailing southerly current along the coast
Development and application of C - scan ultrasonic facility
This paper presents the in-house development and application of a C-scan ultrasonic facility ULTIMA 200M2 at the Indira Gandhi Centre for Atomic Research (IGCAR), Kalpakkam, carried out in collaboration with the Electronics Division, Bhabha Atomic Research Centre (BARC), Mumbai. The paper describes various constituents of the system developed and also highlights the typical results obtained using this system, including bond integrity assessment of explosive welds and imaging of fuel sub-assembly heads of the Fast Breeder Test Reactor. The system has also been used for imaging both the sides of a one rupee Indian coin. All the finer details of the coin could be extracted, demonstrating the resolution capabilities of the system
First experience in the larviculture of cobia, Rachycentron canadum (Linnaeus, 1752) in India
Cobia aquaculture has been gaining momentum internationally and has spread to more than 23 countries, half of them in the
Asia-Pacific region. Envisaging the prospects of cobia farming in India, broodstock development was initiated and the first
successful induced breeding was achieved in March 2010. Larviculture was experimented in Fibre Reinforced Plastic (FRP)
tanks as well as Reinforced Cement Concrete (RCC) tanks and protocols were evolved. Green water technique employing
the microalga, Nannochloropsis oculata was used. The critical stage for the larvae was from 5 to 9 days post-hatch (dph),
when cumulative mortality reached around 90%. Enriched rotifers were fed from 3 to 10 dph and enriched Artemia nauplii
from 9 to 18 dph. Weaning with larval inert feed was initiated from 18 dph and grading was carried out once in four days to
avoid cannibalism. The study was conducted for 31 days and the final larval survival noted in the FRP and RCC tanks were
2 and 1%, respectively. At the end of the experiment, the specific growth rate of larvae in the FRP system was 30.1% of body
weight per day, while the same in RCC tank was 28.3% of body weight per day. The low survival and specific growth rate
of larvae in the RCC tanks could be attributed to the low densities of live feed maintained. The present experience indicated
that cobia seed production can be successfully practised and by refining the methodology, the survival and growth can be
enhanced to achieve commercial level fingerling production
Broodstock development and controlled breeding of cobia Rachycentron canadum (Linnaeus 1766) from Indian seas
Cobia, Rachycentron canadum has emerged as one of the topmost finfish species for mariculture. In India, cobia broodstock
was developed and induced breeding was achieved for the first time at Mandapam Regional Centre of the Central Marine
Fisheries Research Institute (CMFRI). The broodstock was developed in sea cages of 6 m diameter and 3.5 m depth. Sexes
were separated about two months prior to the onset of breeding season and stocked in separate cages. During March 2010, a
female with intra-ovarian egg diameter of 700 ╬╝ along with two males were selected for induced spawning. The brooders
were induced with human chorionic gonadotropin (hCG) at doses of 500 IU per kg body weight for female and 250 IU per
kg body weight for males. Spawning was noted after 39 h of intra-muscular injection. The total eggs spawned were
estimated as 2.1 million. About 90% fertilization was recorded (fertilized eggs amounted to 1.9 million). The eggs were
collected using a 500 ╬╝ mesh net and stocked in incubation tanks at varying densities. The eggs hatched after 22 h of
incubation at a temperature range of 28-30 ┬║C. The percentage of hatching was 80% and the total number of newly hatched
larvae was estimated as 1.5 millio
Larval rearing of mud crab, Scylla tranquebarica (Fabricius, 1798) and feeding requirements of its zoea
Feeding experiments were conducted with zooplankton to find out the suitable food for zoea1 of the mud crab
Scylla tranquebarica. Zoeae1 from seven berried females, three from wild and four from rematured specimens
were the source material for the present study. The veliger larvae (50-55╬╝m) of pearl oyster and two
species (Pseudodiaptomus spp. and Labidocera spp.) of pelagic copepods (500-1000 ╬╝m) were tested as
feed. However, the zoea1 accepted only the former. The feeding activity could be resolved into 5 stages.
Significant role of the serrated caudal setae in prey abrasion into required size suitable to mandibles for
mastication and the size preference of the items of diet in relation to the growth of the mandibles with the
advancement of zoeal stage also have been discussed. In all the 7 feeding experiments that were conducted by
providing rotifer, Brachionus rotundiformis (<100 ╬╝m), the zoea1 advanced to zoea3/zoea4 or the first crab
stage. The present study is a significant development in narrating the feeding behaviour of zoea1
Prawn fauna (Crustacea: Decapoda) of India - An annotated checklist of the Penaeoid, Sergestoid, Stenopodid and Caridean prawns
Twenty four species of Pandalid shrimps reported from the
Indian waters, of which six genera (Chlorotocella, Chlorotocus,
Chlorocurtis, Dorodotes, Heterocarpoides and Stylopandalus)
are represented by single species each. The genera, Plesionika
and Heterocarpus are represented by eleven and seven species
respectively. Plesionika adensameri (Balss, 1914) a deep-sea
shrimp hitherto unreported from Indian waters is recorded from
west coast of India. Information on some biological aspects of
few Pandalid shrimps from Indian waters is also reported in the
present paper
The lncRNA CASC15 regulates SOX4 expression in RUNX1-rearranged acute leukemia
Abstract Background Long non-coding RNAs (lncRNAs) play a variety of cellular roles, including regulation of transcription and translation, leading to alterations in gene expression. Some lncRNAs modulate the expression of chromosomally adjacent genes. Here, we assess the roles of the lncRNA CASC15 in regulation of a chromosomally nearby gene, SOX4, and its function in RUNX1/AML translocated leukemia. Results CASC15 is a conserved lncRNA that was upregulated in pediatric B-acute lymphoblastic leukemia (B-ALL) with t (12; 21) as well as pediatric acute myeloid leukemia (AML) with t (8; 21), both of which are associated with relatively better prognosis. Enforced expression of CASC15 led to a myeloid bias in development, and overall, decreased engraftment and colony formation. At the cellular level, CASC15 regulated cellular survival, proliferation, and the expression of its chromosomally adjacent gene, SOX4. Differentially regulated genes following CASC15 knockdown were enriched for predicted transcriptional targets of the Yin and Yang-1 (YY1) transcription factor. Interestingly, we found that CASC15 enhances YY1-mediated regulation of the SOX4 promoter. Conclusions Our findings represent the first characterization of this CASC15 in RUNX1-translocated leukemia, and point towards a mechanistic basis for its action
Austro-Asiatic Tribes of Northeast India Provide Hitherto Missing Genetic Link between South and Southeast Asia
Northeast India, the only region which currently forms a land bridge between the Indian subcontinent and Southeast Asia, has been proposed as an important corridor for the initial peopling of East Asia. Given that the Austro-Asiatic linguistic family is considered to be the oldest and spoken by certain tribes in India, Northeast India and entire Southeast Asia, we expect that populations of this family from Northeast India should provide the signatures of genetic link between Indian and Southeast Asian populations. In order to test this hypothesis, we analyzed mtDNA and Y-Chromosome SNP and STR data of the eight groups of the Austro-Asiatic Khasi from Northeast India and the neighboring Garo and compared with that of other relevant Asian populations. The results suggest that the Austro-Asiatic Khasi tribes of Northeast India represent a genetic continuity between the populations of South and Southeast Asia, thereby advocating that northeast India could have been a major corridor for the movement of populations from India to East/Southeast Asia
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