After continued harvesting, when the rotiter population declines due to their resorting to sexual reproduction, the culture is rejuvenated in the same pool by renewing 1/3 volume of water and by refertilising. This continous culture with frequent harvesting can be maintained for over 2 months

Manisseri, Mary K

Palanichamy, S

CMFRI Digital Repository

POSTER PAPER CONTINUOUS CULTURE OF ROTIFERS FOR LARVAL REARING OF MARINE PRAWNS MARY K. MANISSERI and S. PALANICHAMY Narakkal Prawn Culture Laboratory of CMFRI, Cochin-682505 A technique for the continuous culture of the euryhaline rotifer Brach;-onus plicatilis has been perfected at the NPCL for the mass production of prawn seeds. Double filtered seawater is pumped into a 40 tonne capacity outdoor plastic lined pool and ferti-lised with groundnut oil cake (200 gm/ton), superphosphate (2 gms/ton) and urea (2 gms/ton). This medium is inoculated the same day with ChIarella culture at the rate of 5 litre/ton and on the second day rotifers are intro-duced at the rate of about 500 per I itre. Vigorous aeration is provided continuously and the culture attains a concentration of 4-5 x 1 05 rotifers per litre in 5 days. Harvesting is done with a hand-net of 60 micron mesh size in the morning hours when they swarm at the surface. About 200 to 250 million rotiters are collected during each harvest. They are concentrated, washed twice with filtered seawater and after mixing with equal volume of 10% glycerin in sea-water are frozen into blocks in a deep freezer. This method of harvesting and preservation ensures a ready stock for use at any time. After continued harvesting, when the rotiter population declines due to their resorting to sexual reproduction, the culture is rejuvenated in the same pool by renewing 1/3 velume Qf wa tef and by ref'IH'lmsing, This 1:ontlnuom~ culture with frequent haNesting can be mAIntained for· OV al' 2 months, 'T"M'El ,~sti,ng eggs produced b'ytMm dlu~ng the se~Ual rep'Foduction a re. collee-ted from the oottom :01· the peol .and the:v are dried and stored for future use. 0 9 u. 

Continuous culture of rotifers for larval rearing of marine prawns

http://eprints.cmfri.org.in/8497/1/Mary_K_Manissery_154.pdf

Continuous culture of rotifers for larval rearing of marine prawns

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