A Climate History of Boone County, Missouri, From Tree-Ring Analysis of Eastern Redcedar

A ring-width index, constructed from analyses of eastern redcedar (Juniperus virginiana L.) trees, for central Boone County, Missouri, is presented. Correlations between summer temperatures, spring rainfall and the index are shown. Some possible interpretations of past climate history, based on the index, are listed for selected time periods back to 1650 AD

A Note on Anatomical Changes of White Oak Wood Upon Exposure to Gamma Radiation

White oak heartwood samples were exposed to 650, 950 and 1900 MRad of cobalt-60 gamma radiation. The holocellulose portion of the heartwood cell walls was degraded while the lignin percentage remained relatively unchanged. Tangential vessel diameter, ray cell length, and length and width of intervessel pits increased upon exposure while tangential vessel-wall thickness, ray cell double-wall thickness, and latewood fiber double-wall thickness decreased

Functional divergence in the role of N-linked glycosylation in smoothened signaling

The G protein-coupled receptor (GPCR) Smoothened (Smo) is the requisite signal transducer of the evolutionarily conserved Hedgehog (Hh) pathway. Although aspects of Smo signaling are conserved from Drosophila to vertebrates, significant differences have evolved. These include changes in its active sub-cellular localization, and the ability of vertebrate Smo to induce distinct G protein-dependent and independent signals in response to ligand. Whereas the canonical Smo signal to Gli transcriptional effectors occurs in a G protein-independent manner, its non-canonical signal employs Gαi. Whether vertebrate Smo can selectively bias its signal between these routes is not yet known. N-linked glycosylation is a post-translational modification that can influence GPCR trafficking, ligand responsiveness and signal output. Smo proteins in Drosophila and vertebrate systems harbor N-linked glycans, but their role in Smo signaling has not been established. Herein, we present a comprehensive analysis of Drosophila and murine Smo glycosylation that supports a functional divergence in the contribution of N-linked glycans to signaling. Of the seven predicted glycan acceptor sites in Drosophila Smo, one is essential. Loss of N-glycosylation at this site disrupted Smo trafficking and attenuated its signaling capability. In stark contrast, we found that all four predicted N-glycosylation sites on murine Smo were dispensable for proper trafficking, agonist binding and canonical signal induction. However, the under-glycosylated protein was compromised in its ability to induce a non-canonical signal through Gαi, providing for the first time evidence that Smo can bias its signal and that a post-translational modification can impact this process. As such, we postulate a profound shift in N-glycan function from affecting Smo ER exit in flies to influencing its signal output in mice

Bottom-current control on sedimentation in the western Bellingshausen Sea, West Antarctica

A set of single channel and multi channel seismic reflection profiles provide insights in the young Cenozoic sedimentation history on the continental rise of the western Bellingshausen Sea west and north of Peter I Island. This area was mainly influenced by the glacial controlled sediment supply from the continental shelf and by bottom current activity. The seismic data show northwards structural altering of a prominent sediment mound from a sediment drift structure into a oppositional orientated large channel-levee complex lying west of an erosional channel. This change indicates a northward decreasing influence of a westward flowing bottom contour current. The topography suggests Peter I Island to be the main feature for the change of the bottom current influence, acting as a barrier for the bottom current and the entrained sediment material. West of Peter I Island the eastward orientated Coriolis force remains as the affecting force which deflects suspended load of the turbidites to the west and leads to a stronger grow of the western channel-levee. Calculated sediment deposition rates based on the seismic data reveal the sediment mound as a remarkable and important sediment depocentre for young Cenozoic glacial transported and contouritic sediment material in the Bellingshausen Sea

The mental health and wellbeing of adolescents on remand in Australia

Objective: To compare the nature and prevalence of mental health problems, prevalence of suicidal ideation and behaviour, and health-related quality of life of 13–17-year-olds on remand with that of 13–17-year-olds in the general community. Method: Self reported questionnaires completed by 13–17-year-olds who were remanded in South Australia in 2008/9 (N = 159), 13–17-year-olds who participated in the Child and Adolescent Component of the National Survey of Mental Health and Well-being in Australia (N = 1283), and 13–17-year-olds who participated in the Western Australian Aboriginal Child Health Survey (N = 1100). Mental health problems were identified using the Youth Self-Report and the Strength and Difficulties Questionnaire. Health-related quality of life was assessed using the Child Health Questionnaire. Results: A total of 50.0% (95% CI, 42.3–58.3) of adolescents on remand versus 18.9% of adolescents in the community scored above the recommended cut-off score on the Youth Self-Report. Among Indigenous adolescents, 55.8% (95% CI, 41.3–69.5) on remand versus 32.1% in the community scored above the recommended cut-off score on the Strength and Difficulties Questionnaire. Among those on remand, 19.1% (95% CI, 13.2–26.2) reported making a suicide attempt during the previous 12 months compared to 4.3% in the community. Health-related quality of life was significantly worse among adolescents on remand than adolescents in the community. These differences remained after adjusting for differences in the demographic characteristics of the two groups of adolescents. Conclusions: Compared with adolescents in the community, both Indigenous and non-Indigenous adolescents on remand experience a wide range of problems, including poorer mental and physical health, a higher prevalence of suicidal ideation and behaviour, greater family adversity, and poorer school attendance. This broad range of problems needs to be effectively addressed to enable adolescents on remand to become active and productive members of their communities.Michael G. Sawyer, Matthew Guidolin, Karen L. Schulz, Bernie McGinnes, Stephen R. Zubrick, Peter A. Baghurs

An effective strategy for influenza vaccination of healthcare workers in Australia: experience at a large health service without a mandatory policy

BACKGROUND: Annual influenza vaccination of healthcare workers (HCWs) is recommended in Australia, but uptake in healthcare facilities has historically been low (approximately 50%). The objective of this study was to develop and implement a dedicated campaign to improve uptake of staff influenza annual vaccination at a large Australian health service. METHODS: A quality improvement program was developed at Alfred Health, a tertiary metropolitan health service spanning 3 campuses. Pre-campaign evaluation was performed by questionnaire in 2013 to plan a multimodal vaccination strategy. Reasons for and against vaccination were captured. A campaign targeting clinical and non-clinical healthcare workers was then implemented between March 31 and July 31 2014. Proportional uptake of influenza vaccination was determined by campus and staff category. RESULTS: Pre-campaign questionnaire responses were received from 1328/6879 HCWs (response rate 20.4%), of which 76% were vaccinated. Common beliefs held by unvaccinated staff included vaccine ineffectiveness (37.1%), that vaccination makes staff unwell (21.0%), or that vaccination is not required because staff are at low risk for acquiring influenza (20.2%). In 2014, 6009/7480 (80.3%) staff were vaccinated, with significant improvement in uptake across all campuses and amongst nursing, medical and allied health staff categories from 2013 to 2014 (p < 0.0001). CONCLUSIONS: A non-mandatory multimodal strategy utilising social marketing and a customised staff database was successful in increasing influenza vaccination uptake by all staff categories. The sustainability of dedicated campaigns must be evaluated