Laterality of Eye Use by Bottlenose (Tursiops truncatus) and Rough-toothed (Steno bredanensis) Dolphins While Viewing Predictable and Unpredictable Stimuli

Laterality of eye use has been increasingly studied in cetaceans. Research supports that many cetacean species keep prey on the right side while feeding and preferentially view unfamiliar objects with the right eye. In contrast, the left eye has been used more by calves while in close proximity to their mothers. Despite some discrepancies across and within species, laterality of eye use generally indicates functional specialization of brain hemispheres in cetaceans. The present study aimed to examine laterality of eye use in bottlenose dolphins (Tursiops truncatus) and rough-toothed dolphins (Steno bredanensis) under managed care. Subjects were video-recorded through an underwater window while viewing two different stimuli, one predictable and static and the other unpredictable and moving. Bottlenose dolphins displayed an overall right-eye preference, especially while viewing the unpredictable, moving stimulus. Rough-toothed dolphins did not display eye preference while viewing stimuli. No significant correlations between degree of laterality and behavioral interest in the stimuli were found. Only for bottlenose dolphins were the degree of laterality and curiosity ratings correlated. This study extends research on cetacean lateralization to a species not extensively examined and to stimuli that varied in movement and degree of predictability. Further research is needed to make conclusions regarding lateralization in cetaceans

Precursor ion scanning for detection and structural characterization of heterogeneous glycopeptide mixtures

AbstractThe structure of N-linked glycans is determined by a complex, anabolic, intracellular pathway but the exact role of individual glycans is not always clear. Characterization of carbohydrates attached to glycoproteins is essential to aid understanding of this complex area of biology. Specific mass spectral detection of glycopeptides from protein digests may be achieved by on-line HPLC-MS, with selected ion monitoring (SIM) for diagnostic product ions generated by cone voltage fragmentation, or by precursor ion scanning for terminal saccharide product ions, which can yield the same information more rapidly. When glycosylation is heterogeneous, however, these approaches can result in spectra that are complex and poorly resolved. We have developed methodology, based around precursor ion scanning for ions of high m/z, that allows site specific detection and structural characterization of glycans at high sensitivity and resolution. These methods have been developed using the standard glycoprotein, fetuin, and subsequently applied to the analysis of the N-linked glycans attached to the scrapie-associated prion protein, PrPSc. These glycans are highly heterogeneous and over 30 structures have been identified and characterized site specifically. Product ion spectra have been obtained on many glycopeptides confirming structure assignments. The glycans are highly fucosylated and carry Lewis X or sialyl Lewis X epitopes and the structures are in-line with previous results. [Abbreviations: Hex–Hexose, C6H12O6 carbohydrates, including mannnose and galactose; HexNAc—N-acetylhexosamine, C8H15NO6 carbohydrates, including N-acetylglucosamine and N-acetylgalactosamine; GlcNAc—N-acetylglucosamine; GalNAc—N-acetylgalactosamine; Fuc–Fucose; NeuAC—N-acetylneuraminic acid or sialic acid; TSE—Transmissible Spongiform Encephalopathy.

Formation of Phase Space Holes and Clumps

It is shown that the formation of phase space holes and clumps in kinetically driven, dissipative systems is not restricted to the near threshold regime, as previously reported and widely believed. Specifically, we observe hole-clump generation from the edges of an unmodulated phase space plateau, created via excitation, phase mixing and subsequent dissipative decay of a linearly unstable bulk plasma mode in the electrostatic bump-on-tail model. This has now allowed us to elucidate the underlying physics of the hole-clump formation process for the first time. Holes and clumps develop from negative energy waves that arise due to the sharp gradients at the interface between the plateau and the nearly unperturbed, ambient distribution and destabilize in the presence of dissipation in the bulk plasma. We confirm this picture by demonstrating that the formation of such nonlinear structures in general does not rely on a "seed" wave, only on the ability of the system to generate a plateau. In addition, we observe repetitive cycles of plateau generation and erosion, the latter due to hole-clump formation and detachment, which appear to be insensitive to initial conditions and can persist for a long time. We present an intuitive discussion of why this continual regeneration occurs

Out of the Mouth of Babes: Lessons from Research on Human Infants

Marine mammal behavior and cognition researchers often face a number of challenges, including the research subjects’ lack of interest and verbal abilities, as well as choosing a paradigm with appropriate stimuli for the subjects’ perceptual and cognitive abilities. Researchers who work with human infants often encounter similar challenges when studying infant cognition and have developed strategies to overcome these challenges, including using stimuli that capture the infants’ attention, determining what tasks are age-appropriate, and using conditioned responses to test discrimination abilities. This paper encourages marine mammal researchers to learn from the research paradigms and techniques used in human infant research and alter them appropriately for the intended study subjects. The conditioned head-turn response, Violation-of-Expectation paradigm, and the help/hinder paradigm have all been used in infant cognition research and show great promise for furthering the current understanding of marine mammal behavior and cognition. In addition, studying a subject’s spontaneous behavior can provide valuable insight in areas such as problem solving skills, creativity, and individual differences. Care must be taken to adapt the paradigms and use stimuli to fit each species’ perceptual abilities. For example, avoiding a task that requires color discrimination for species that do not possess color vision or using stimuli that fall within a particular species’ hearing range are necessary steps in designing an ecologically valid and informative study. Adapting paradigms previously used with human infants can help expand the current understanding of marine mammal communication, cognitive abilities, and social behavior

Investigations of flowfields found in typical combustor geometries

The flowfields of gas turbine combustion chambers were investigated. Six flowfield configurations with sidewall angles alpha = 90 and 45 deg. and swirl vane angles phi = 0, 45 and 70 deg. are characterized. Photography of neutrally-buoyant helium-filled soap bubbles, tufts, and injected smoke helps to characterize the time-mean streamlines, recirculation zones and regions of highly turbulent flow. Five-hole pitot probe pressure measurements allow the determination of time-mean velocities u, v and w. An advanced computer code equipped with a standard two-equation kappa-epsilon turbulence model was used to predict corresponding flow situations and to compare results with the experimental data

Do Pinnipeds Have Personality? Broad Dimensions and Contextual Consistency of Behavior in Harbor Seals (Phoca vitulina) and California Sea Lions (Zalophus californianus)

Personality has now been studied in species as diverse as chimpanzees and cuttlefish, but marine mammals remain vastly underrepresented in this area. A broad range of traits have been assessed only once in each of bottlenose dolphins and California sea lions, while consistent individual differences in a few specific behaviors have been identified in grey seals. Furthermore, the context component of definitions of personality is not often assessed, despite evidence that animals may show individual patterns of behavioral consistency across contexts. The current study therefore aimed to use behavioral coding to assess underlying personality factors and consistency across contexts in two marine mammal species: California sea lions and harbor seals. In both species, two personality factors were extracted using exploratory factor analysis. Both were broadly similar across species; the first, Boldness, resembled human Extraversion, and to some extent Openness, with sea lions exhibiting a greater social component. The second factor was labeled Routine Activity, and may contain some Conscientiousness-like traits. Species-specific patterns were also identified for interactive behaviors across two contexts. However, there was substantial individual variation in the frequency of these behaviors, as well as some animals who did not conform to group-level trends. This study therefore provides novel evidence for broad personality factors and both groupand individual-level patterns of contextual consistency in two pinniped species

Laterality of Eye Use by Bottlenose (Tursiops truncatus) and Rough-toothed (Steno bredanensis) Dolphins While Viewing Predictable and Unpredictable Stimuli

Laterality of eye use has been increasingly studied in cetaceans. Research supports that many cetacean species keep prey on the right side while feeding and preferentially view unfamiliar objects with the right eye. In contrast, the left eye has been used more by calves while in close proximity to their mothers. Despite some discrepancies across and within species, laterality of eye use generally indicates functional specialization of brain hemispheres in cetaceans. The present study aimed to examine laterality of eye use in bottlenose dolphins (Tursiops truncatus) and rough-toothed dolphins (Steno bredanensis) under managed care. Subjects were video-recorded through an underwater window while viewing two different stimuli, one predictable and static and the other unpredictable and moving. Bottlenose dolphins displayed an overall right-eye preference, especially while viewing the unpredictable, moving stimulus. Rough-toothed dolphins did not display eye preference while viewing stimuli. No significant correlations between degree of laterality and behavioral interest in the stimuli were found. Only for bottlenose dolphins were the degree of laterality and curiosity ratings correlated. This study extends research on cetacean lateralization to a species not extensively examined and to stimuli that varied in movement and degree of predictability. Further research is needed to make conclusions regarding lateralization in cetaceans

Seasonality of Social Behaviour Among Immature Belugas (Delphinapterus leucas) in Managed Care

Belugas ( Delphinapterus leucas ) in managed care have been reported to show seasonal variation in socio-sexual behaviour, hormone levels and respiration rates; however, little is known about the social interactions of wild belugas when they are not in summer, near-shore congregations. To better understand if belugas show seasonal variation in social interactions, this study recorded the behaviour of 10 belugas (five females, five males, ranging from birth to 10 years of age) housed in managed care. Social interactions typically peaked in the summer months but persisted at very low levels during the rest of the year. Sea - sonal variation was most dramatic for socio-sexual behaviour but was generally mirrored in pattern by agonistic and affiliative interactions. Subjects closer to maturity displayed more seasonal variation than younger subjects, and males displayed more seasonal variation compared to females. The peak in social interactions found in this study aligns rather closely with wild belugas’ sum - mer, near-shore congregations, where belugas have increased opportunities for socializing. Although belugas in managed care do not experience a seasonal change in habitat, they do show seasonal changes in social behaviour, which are likely driven by seasonal fluctuations in hormone levels. It is therefore expected that wild beluga populations would show similar behavioural pat - terns if they were observed throughout the remainder of the year. This research has applications for belugas in managed care and may provide a framework for understanding the social behaviour of wild belugas

DNA cruciform arms nucleate through a correlated but non-synchronous cooperative mechanism

Inverted repeat (IR) sequences in DNA can form non-canonical cruciform structures to relieve torsional stress. We use Monte Carlo simulations of a recently developed coarse-grained model of DNA to demonstrate that the nucleation of a cruciform can proceed through a cooperative mechanism. Firstly, a twist-induced denaturation bubble must diffuse so that its midpoint is near the centre of symmetry of the IR sequence. Secondly, bubble fluctuations must be large enough to allow one of the arms to form a small number of hairpin bonds. Once the first arm is partially formed, the second arm can rapidly grow to a similar size. Because bubbles can twist back on themselves, they need considerably fewer bases to resolve torsional stress than the final cruciform state does. The initially stabilised cruciform therefore continues to grow, which typically proceeds synchronously, reminiscent of the S-type mechanism of cruciform formation. By using umbrella sampling techniques we calculate, for different temperatures and superhelical densities, the free energy as a function of the number of bonds in each cruciform along the correlated but non-synchronous nucleation pathways we observed in direct simulations.Comment: 12 pages main paper + 11 pages supplementary dat

Comparative muscle development of scyphozoan jellyfish with simple and complex life cycles

International audienceBackground : Simple life cycles arise from complex life cycles when one or more developmental stages are lost. This raises a fundamental question - how can an intermediate stage, such as a larva, be removed, and development still produce a normal adult? To address this question, we examined the development in several species of pelagiid jellyfish. Most members of Pelagiidae have a complex life cycle with a sessile polyp that gives rise to ephyrae (juvenile medusae); but one species within Pelagiidae, Pelagia noctiluca, spends its whole life in the water column, developing from a larva directly into an ephyra. In many complex life cycles, adult features develop from cell populations that remain quiescent in larvae, and this is known as life cycle compartmentalization and may facilitate the evolution of direct life cycles. A second type of metamorphic processes, known as remodeling, occurs when adult features are formed through modification of already differentiated larval structures. We examined muscle morphology to determine which of these alternatives may be present in Pelagiidae.Results : We first examined the structure and development of polyp and ephyra musculature in Chrysaora quinquecirrha, a close relative of P. noctiluca with a complex life cycle. Using phallotoxin staining and confocal microscopy, we verified that polyps have four to six cord muscles that persist in strobilae and discovered that cord muscles is physically separated from ephyra muscle. When cord muscle is removed from ephyra segments, normal ephyra muscle still develops. This suggests that polyp cord muscle is not necessary for ephyra muscle formation. We also found no evidence of polyp-like muscle in P. noctiluca. In both species, we discovered that ephyra muscle arises de novo in a similar manner, regardless of the life cycle.Conclusions : The separate origins of polyp and ephyra muscle in C. quinquecirrha and the absence of polyp-like muscle in P. noctiluca suggest that polyp muscle is not remodeled to form ephyra muscle in Pelagiidae. Life cycle stages in Scyphozoa may instead be compartmentalized. Because polyp muscle is not directly remodeled, this may have facilitated the loss of the polyp stage in the evolution of P. noctiluca