75 research outputs found

    Introduction of electric vehicle charging stations to university campuses : A case study for the university of Georgia from 2014 to 2017

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    Electric vehicles (EVs) are becoming increasingly popular in the United States of America (USA). EVs attract buyers with benefits including energy efficiency and environmental friendliness. As EV usage grows, more public spaces are installing EV charging stations. This paper presents a comprehensive analysis of EV charging station usage at the University of Georgia (UGA) in Athens, Georgia. Three ChargePoint EV charging stations at UGA were used to collect data about each of 3204 charging events that occurred from 10 April 2014 to 20 June 2017. The charging event data included start date, start time, length of parking time, length of charging time, amount of energy delivered, and the postal code entered by the user during ChargePoint account registration. Analytical methods were proposed to obtain information about EV charging behavior, charging station occupancy, and geolocation of charging station users. The methodology presented here was time- and cost-effective, as well as scalable to other organizations that own charging stations. Because this study took place at a university, the results presented here can be used as a reference for EV charging station usage in other college towns in the USA that do not have EV charging stations but are planning to develop EV infrastructure

    Textile sensors for stab and cut detection

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    Manufacturers are aiming for more flexible and lightweight protective clothing to increase wearing comfort. A cardigan with a knitted stab-resistant inlay and an alarm system is presented. The stab-resistant inlay is based on a multilayer ultra-high molecular weight poly ethylene (UHMW-PE) fabric. Stab resistance was evaluated according to the standard of the Association of Test Laboratories for Bullet, Stab or Pike Resistant Materials and Construction Standard (VPAM 2011). Furthermore sensors for the detection of cuts and pressure were integrated. Both sensors can trigger alarms if the wearer is attacked. Normal pressure occurring through leaning on a wall or sitting is filtered out and does not trigger an alarm

    Expression of High-Affinity IgE Receptor on Human Peripheral Blood Dendritic Cells in Children

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    BACKGROUND: In a mouse model of viral induced atopic disease, expression of FcεRI on dendritic cells is critical. While adult human conventional (cDC) and plasmacytoid (pDC) dendritic cells have been shown to express FcεRI, it is not known if this receptor is expressed in childhood and how its expression is governed by IgE. METHODS: Following informed consent of subjects (n = 27, aged 12-188 months), peripheral blood was stained for surface expression of CD19, ILT7, CD1c, IgE, FcεRI and analyzed by flow cytometry (cDC: CD19(-) ILT7(-) CD1c(+); pDC: CD19(-) ILT7(+) CD1c(-)). Total and specific serum IgE levels to food and inhalant allergens were determined by ImmunoCAP, and the relationship between FcεRI expression on dendritic cells and sensitization, free IgE, cell bound IgE, and age was determined. RESULTS: Independent of sensitization status, FcεRI expression was noted on cDC and pDC as early as 12 months of age. Serum IgE level correlated with expression of FcεRI on cDC, but not pDC. Based on the concentration of IgE, a complex relationship was found between surface bound IgE and expression of FcεRI on cDC. pDC exhibited a linear relationship of FcεRI expression and bound IgE that was consistent through all IgE concentrations. CONCLUSIONS: In children, FcεRI expression on cDC and pDC is modulated differently by serum and cell bound IgE. IgE governance of FcεRI expression on cDC depends upon a complex relationship. Further studies are needed to determine the functional roles of FcεRI on cDC and pDC

    Histologische, funktionelle und spezifische Regeneration nach Durchtrennung der Fila olfactoria beim Goldfisch ( Carassius auratus )

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    1. Alter dissection of the olfactory libres (fila olfactoria, Fig. 1) in the goldfisch, histological (Fig. 5) as well as functional regeneration takes place. When regeneration of the olfactory fibres was complete, no differences in training and learning behaviour between operated and untreated control animals could be observed (Fig. 2). Specific regeneration could also be proved after dissection of the olfactory fibres: Preoperatively trained discriminative behaviour returned (Figs. 3, 4) when histological regeneration had reached an advanced state (Figs. 6, 7). Higher concentrations of all substances used for odour training were capable of exciting the taste receptors or other struktures outside the olfactory mucosa. The concentrations used for olfactory training, however, were below the thresholds for non-olfactory perception. Neither the surgical procedure nor the anesthesia influenced the memory function. 1. Goldfische können die durchschnittenen Bahnen der Fila olfactoria anatomisch und funktionell regenerieren.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/47102/1/359_2004_Article_BF00340911.pd

    The development and general morphology of the telencephalon of actinopterygian fishes: synopsis, documentation and commentary

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    The Actinopterygii or ray-finned fishes comprise, in addition to the large superorder of teleosts, four other superorders, namely the cladistians, the chondrosteans, the ginglymodes, and the halecomorphs, each with a limited number of species. The telencephalon of actinopterygian fishes differs from that in all other vertebrates in that it consists of a pair of solid lobes. Lateral ventricles surrounded by nervous tissue are entirely lacking. At the end of the nineteenth century, the theory was advanced that the unusual configuration of the forebrain in actinopterygians results from an outward bending or eversion of its lateral walls. This theory was accepted by some authors, rejected or neglected by others, and modified by some other authors. The present paper is based on the data derived from the literature, complemented by new observations on a large collection of histological material comprising specimens of all five actinopterygian superorders. The paper consists of three parts. In the first, a survey of the development of the telencephalon in actinopterygian fishes is presented. The data collected show clearly that an outward bending or eversion of the pallial parts of the solid hemispheres is the principal morphogenetic event in all five actinopterygian superorders. In all of these superorders, except for the cladistians, eversion is coupled with a marked thickening of the pallial walls. In the second part, some aspects of the general morphology of the telencephalon in mature actinopterygians are highlighted. It is pointed out that (1) the degree of eversion varies considerably among the various actinopterygian groups; (2) eversion leads to the transformation of the telencephalic roof plate into a wide membrane or tela choroidea, which is bilaterally attached to the lateral or ventrolateral aspect of the solid hemispheres; (3) the lines of attachment or taeniae of the tela choroidea form the most important landmarks in the telencephalon of actinopterygians, indicating the sites where the greatly enlarged ventricular surface of the hemispheres ends and its reduced meningeal surface begins; (4) the meningeal surface of the telencephalon shows in most actinopterygians bilaterally a longitudinally oriented sulcus externus, the depth of which is generally positively correlated with the degree of eversion; (5) a distinct lateral olfactory tract, occupying a constant topological position close to the taenia, is present in all actinopterygians studied; and (6) this tract is not homologous to the tract of the same name in the evaginated and inverted forebrains of other groups of vertebrates. In the third and final section, the concept that the structural organization of the pallium in actinopterygians can be fully explained by a simple eversion of its walls, and the various theories, according to which the eversion is complicated by extensive shifts of its constituent cell groups, are discussed and evaluated. It is concluded that there are no reasons to doubt that the pallium of actinopterygian fishes is the product of a simple and complete eversion

    Semi-automated assembly of high-quality diploid human reference genomes

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    The current human reference genome, GRCh38, represents over 20 years of effort to generate a high-quality assembly, which has benefitted society. However, it still has many gaps and errors, and does not represent a biological genome as it is a blend of multiple individuals. Recently, a high-quality telomere-to-telomere reference, CHM13, was generated with the latest long-read technologies, but it was derived from a hydatidiform mole cell line with a nearly homozygous genome. To address these limitations, the Human Pangenome Reference Consortium formed with the goal of creating high-quality, cost-effective, diploid genome assemblies for a pangenome reference that represents human genetic diversity. Here, in our first scientific report, we determined which combination of current genome sequencing and assembly approaches yield the most complete and accurate diploid genome assembly with minimal manual curation. Approaches that used highly accurate long reads and parent-child data with graph-based haplotype phasing during assembly outperformed those that did not. Developing a combination of the top-performing methods, we generated our first high-quality diploid reference assembly, containing only approximately four gaps per chromosome on average, with most chromosomes within ±1% of the length of CHM13. Nearly 48% of protein-coding genes have non-synonymous amino acid changes between haplotypes, and centromeric regions showed the highest diversity. Our findings serve as a foundation for assembling near-complete diploid human genomes at scale for a pangenome reference to capture global genetic variation from single nucleotides to structural rearrangements

    Klinische Relevanz der Sensibilisierung gegen Schimmelpilze

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    Ex-Vivo Gewebekultur von Nasenschleimhaut mittels Oxygenierung

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