Observations on Livestock Productivity in Sheep Fed Exclusively on Haulms from Ten Different Genotypes of Groundnut

Haulms from 10 different genotypes of groundnut were tested with growing male sheep for digestibility, intake, nitrogen retention and live weight gains. Very high daily intake levels of > 4% of sheep bodyweight were observed in all haulms. Nitrogen retention (range: 6.7 to 11.4 g/d) and live weight gains (range: 65 to 137 g/d) varied about two fold among genotypes suggesting that the choice of groundnut genotype can have a very significant effect on livestock productivity in feeding systems based on groundnut haulms. Haulm fodder quality difference were well reflected by acid detergent lignin (ADL), which accounted for 58, 39 and 72% of the variation in haulm in vivo digestibility, nitrogen retention and live weight gains, respectively. Using stepwise multiple regression procedures, combinations of haulm ADL content and in vitro digestibility and of haulm ADL content and in vitro metabolisable energy content accounted for 84% and 92% of the variation in in vivo digestibility and live weight gain, respectively. The combination of haulm nitrogen and ADL content accounted for 80% of the variation in nitrogen retention. Among the genotypes, ICGV 89104), ICGV 91114), TMV 2 and ICGV 92093 promoted highest productivity in shee

90Y Radioembolization for Hepatic Malignancy in Patients with Previous Biliary Intervention: Multicenter Analysis of Hepatobiliary Infections

PurposeTo determine the frequency of hepatobiliary infections after transarterial radioembolization (TARE) with yttrium 90 (90Y) in patients with liver malignancy and a history of biliary intervention.Materials and MethodsFor this retrospective study, records of all consecutive patients with liver malignancy and history of biliary intervention treated with TARE at 14 centers between 2005 and 2015 were reviewed. Data regarding liver function, 90Y dosimetry, antibiotic prophylaxis, and bowel preparation prophylaxis were collected. Primary outcome was development of hepatobiliary infection.ResultsOne hundred twenty-six patients (84 men, 42 women; mean age, 68.8 years) with primary (n = 39) or metastatic (n = 87) liver malignancy and history of biliary intervention underwent 180 procedures with glass (92 procedures) or resin (88 procedures) microspheres. Hepatobiliary infections (liver abscesses in nine patients, cholangitis in five patients) developed in 10 of the 126 patients (7.9%) after 11 of the 180 procedures (6.1%; nine of those procedures were performed with glass microspheres). All patients required hospitalization (median stay, 12 days; range, 2–113 days). Ten patients required percutaneous abscess drainage, three patients underwent endoscopic stent placement and stone removal, and one patient needed insertion of percutaneous biliary drains. Infections resolved in five patients, four patients died (two from infection and two from cancer progression while infection was being treated), and one patient continued to receive suppressive antibiotics. Use of glass microspheres (P = .02), previous liver resection or ablation (P = .02), and younger age (P = .003) were independently predictive of higher infection risk.ConclusionInfectious complications such as liver abscess and cholangitis are uncommon but serious complications of transarterial radioembolization with 90Y in patients with liver malignancy and a history of biliary intervention.© RSNA, 2018Online supplemental material is available for this article

Nucleolin Inhibits G4 Oligonucleotide Unwinding by Werner Helicase

The Werner protein (WRNp), a member of the RecQ helicase family, is strongly associated with the nucleolus, as is nucleolin (NCL), an important nucleolar constituent protein. Both WRNp and NCL respond to the effects of DNA damaging agents. Therefore, we have investigated if these nuclear proteins interact and if this interaction has a possible functional significance in DNA damage repair.Here we report that WRNp interacts with the RNA-binding protein, NCL, based on immunoprecipitation, immunofluorescent co-localization in live and fixed cells, and direct binding of purified WRNp to nucleolin. We also map the binding region to the C-terminal domains of both proteins. Furthermore, treatment of U2OS cells with 15 µM of the Topoisomerase I inhibitor, camptothecin, causes the dissociation of the nucleolin-Werner complex in the nucleolus, followed by partial re-association in the nucleoplasm. Other DNA damaging agents, such as hydroxyurea, Mitomycin C, and aphidicolin do not have these effects. Nucleolin or its C-terminal fragment affected the helicase, but not the exonuclease activity of WRNp, by inhibiting WRN unwinding of G4 tetraplex DNA structures, as seen in activity assays and electrophoretic mobility shift assays (EMSA).These data suggest that nucleolin may regulate G4 DNA unwinding by WRNp, possibly in response to certain DNA damaging agents. We postulate that the NCL-WRNp complex may contain an inactive form of WRNp, which is released from the nucleolus upon DNA damage. Then, when required, WRNp is released from inhibition and can participate in the DNA repair processes

Malignant Transformation of an Aneurysmal Bone Cyst to Fibroblastic Osteosarcoma.

Aneurysmal bone cysts are uncommon primary bone tumors typically regarded as histologically and clinically benign. Malignant transformation of these lesions occurs almost exclusively in the context of prior radiation exposure. However, 4 cases of an osteosarcoma developing without prior radiation exposure have been reported. In this article, we report a fifth case of degeneration of an aneurysmal bone cyst to a fibroblastic osteosarcoma. In addition to reviewing the earlier cases, we describe the radiologic, pathologic, and immunohistochemical basis of this diagnosis

Malignant Transformation of an Aneurysmal Bone Cyst to Fibroblastic Osteosarcoma.

Aneurysmal bone cysts are uncommon primary bone tumors typically regarded as histologically and clinically benign. Malignant transformation of these lesions occurs almost exclusively in the context of prior radiation exposure. However, 4 cases of an osteosarcoma developing without prior radiation exposure have been reported. In this article, we report a fifth case of degeneration of an aneurysmal bone cyst to a fibroblastic osteosarcoma. In addition to reviewing the earlier cases, we describe the radiologic, pathologic, and immunohistochemical basis of this diagnosis

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Not AvailableA wheat association mapping population consisting of 287 diverse spring wheat lines were evaluated for three years in one location (Varanasi) and out of these for one year across three locations (Karnal, Dharwad and Varanasi) in India. Straw fodder quality traits analyzed were nitrogen (N) content, neutral (NDF) and acid (ADF) detergent fiber, acid detergent lignin (ADL), ash (ASH), in vitro organic matter digestibility (IVOMD) and metabolizable energy (ME) content. Grain yield (GY) and straw yield (SY) were also recorded. Highly significant (P < 0.0001) differences among lines were observed for all traits except for ADF and ADL in the three years trials conducted at Varanasi. However, year and location had strong (P < 0.0001) effects on all traits. Compared to line-dependent variations in GY and SY variation in straw fodder quality traits were small. Proportionally greatest variations between lines were observed for straw N where lowest and highest N varied by about 30%. Difference for NDF and ADF between lines were at most 4% units and below 3% units for IVOMD. Grain yield and straw yield were positively correlated (P < 0.0001) with GY accounting for 26% of the variation in SY. Straw N, IVOMD and ME were weakly but significantly (P < 0.05) negatively associated with GY and SY. Straw NDF and ADF were significantly (P < 0.05) positively correlated with GY but the association was again weak. Straw NDF, ADF and ADL were also weakly but significantly positively correlated with SY. Genome-wide association studies (GWAS) were applied to detect significant marker- straw fodder quality trait associations. Five genomic regions contributed for six traits (ADF, ADL, ASH, IVOMD, ME and NDF). ADF and ADL mapped in the common QTL region on chromosome 2B. Similarly, for the IVOMD and ME QTLs on chromosome 5B were associated with SNP marker, wsnp_Ku_c35090_44349517. While some associations were detected for ADF, ALD, ASH, IVOMD, ME and NDF on chromosomes 1A, 2B, 3A, 5A and 5B, the phenotypic variation explained was low to medium by individual QTL. A likely contributing factor was the comparatively small difference in straw fodder quality traits among the lines. It is interesting to note that line dependent variations in GY and SY were about two-fold. In other words, strong genotypic variations of GY and SY do exist. The lack of any similar variations in straw fodder quality traits is intriguing and requires further research.Not Availabl