189 research outputs found
A cRNA Probe Detects Prunus Necrotic Ringspot Virus in Three Peach Cultivars after Micrografting and in Peach Shoots following Long-term Culture at 4°C
As part of a program to develop transgenic peach (Prunus persica L. Batsch) cultivars with resistance to Prunus necrotic ringspot virus (PNRSV), we are testing a system for measuring virus in peach shoot cultures. Micrografting in vitro is used for inoculation and slot-blot hybridization, with a digoxigenin (DIG)-labeled cRNA probe complementary to the 5´ open reading frame (ORF) of PNRSV RNA 3, for detection. In this study, we investigated whether infected shoots maintain virus infection over long periods of culture at 4°C and if PNRSV-infected ‘Suncrest’ shoot cultures can serve as graft bases to transmit virus equally well into cultivars Nemaguard, Springcrest, and Suncrest. The results of RNA hybridization analysis showed that virus was present in extracts of leaf samples from 2-year-old PNRSV-infected ‘Suncrest’ shoots that had been subjected to varying lengths of incubation at 4 °C in the dark, suggesting that infected shoots can be maintained for repeated use. Rates of graft success were higher in heterografts between ‘Suncrest’ bases and tips of ‘Springcrest’ or ‘Nemaguard’ than in autografts between ‘Suncrest’ and ‘Suncrest’, and there was equal efficacy of graft inoculation from ‘Suncrest’ into these three cultivars
Process approach: the way to improve the quality of high-tech products
В статье представлен процесс регламентации бизнес-процесса «Изготовление источников питания», описанного с использованием методологии IDEF0 и программного продукта Business Studio 4.0 на примере подразделения, которое занимается разработкой и изготовлением сварочных источников тока. При моделировании процесса изготовления источников питания была построена матрица атрибутов, в которой определены цели и задачи процессов, владельцы на каждом этапе процесса, несущие ответственность за ход и за результат, определены входы и выходы (документы) процесса, а также критерии результативности и/или эффективности процесса, для осуществления оперативного управления на всех этапах изготовления конечной продукции. В результате работы был разработан комплект документов, в состав которого вошли: регламент процесса изготовления источников тока, положение о подразделении, должностные инструкции сотрудников, участвующих в процессе. Деятельность по регламентации процесса проводилась в рамках подготовки подразделения к сертификации в соответствии с требованиями стандарта ГОСТ РВ 0015-002-2012 «Система разработки и постановки продукции на производство военной техники. Системы менеджмента качества. Общие требования» в системе добровольной сертификации «Военный регистр»
Comparative assessment of ambient air quality in two urban areas adjacent to petroleum downstream/upstream facilities in Kuwait
The Translation Initiation Factor 3f (eIF3f) Exhibits a Deubiquitinase Activity Regulating Notch Activation
The translation initiation factor complex eIF3f has an intrinsic deubiquitinase activity and regulates the Notch signaling pathway
Colon Capsule Endoscopy compared to Conventional Colonoscopy under routine screening conditions
Colonoscopy (CSPY) for colorectal cancer screening has several limitations. Colon Capsule Endoscopy (PillCam Colon, CCE) was compared to CSPY under routine screening conditions
Concordance and limits between transcutaneous and arterial carbon dioxide pressure in emergency department patients with acute respiratory failure: a single-center prospective observational study
Reproductive health care for asylum-seeking women - a challenge for health professionals
Photoplethysmography respiratory rate monitoring in patients receiving procedural sedation and analgesia for upper gastrointestinal endoscopy
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