Thermal-mechanical response to simple shear extension

The mechanism of extension in the continental crust is apparently much more complex than that acting in the oceanic lithosphere. Recently, Wernicke has proposed that a significant fraction of extension in the continental lithosphere may occur by a simple shear mechanism along discrete fault/shear zones which cut the crust, and perhaps extend into the uppermost mantle. Clearly much of the surface evidence for extension supports this concept, but the depth extent of simple shear extension in the continental crust is unclear. Using numerical simulations, the thermal and associated mechanical behavior of the continental lithosphere in response to lithosphere extension along a low-angle simple shear zone which cuts through the lithospheric plate was determined in order to evaluate the resolving ability of thermal (heat flow and metamorphic P-T-time paths) and elevation observations in constraining the mode of continental extension

A lifting surface computer code with jet-in-crossflow interference effects. Volume 1: Theoretical description

A method is proposed to combine a numerical description of a jet in a crossflow with a lifting surface panel code to calculate the jet/aerodynamic-surface interference effects on a V/STOL aircraft. An iterative technique is suggested that starts with a model for the properties of a jet/flat plate configuration and modifies these properties based on the flow field calculated for the configuration of interest. The method would estimate the pressures, forces, and moments on an aircraft out of ground effect. A first-order approximation to the method suggested is developed and applied to two simple configurations. The first-order approximation is a noniterative precedure which does not allow for interactions between multiple jets in a crossflow and also does not account for the influence of lifting surfaces on the jet properties. The jet/flat plate model utilized in the examples presented is restricted to a uniform round jet injected perpendicularly into a uniform crossflow for a range of jet-to-crossflow velocity ratios from three to ten

Gaining clinical wisdom from adversity: Nurse leaders’ ethical conflict and resilience experiences

Developing effective nurse leaders in today’s“reengineered” corporate healthcare cultures requires that more attention be given to the preparation of ethical leadership during undergraduate programs and nurses’ formative years in practice. Through analyzing the experiences of three nurse leaders dealing with ethical conflicts and the meaning they made of these experiences, we identified that nurses need practical guidance to successfully negotiate ethical conflicts, take risks to uphold their ethical codes, and foster collaborative relationships. According to these nurse leaders, it was important to mentor student and novice nurses in working through workplace ethical conflicts and help guide them through the formulation of successful practical strategies. Our findings showed that these nurses developed resilience and clinical wisdom which they carried through to their leadership practice and future situations

Effect of insecticides on Trichogramma chilonis L., egg parasitoid of large cabbage moth, Crocidolomia pavonana F.

The study was carried out to examine the effects of key insecticides against Trichogramma chilonis parasitism of large cabbage moth (LCM). Three days after spraying with AttackTM, OrtheneTM and EntrustTM (permethrin + pirimiphos-methyl, acephate and spinosad), no parasitism of LCM eggs occurred. After 3 days of Bacillus thuringiensis (Bt) treatment, parasitism of LCM egg mass was 100 %, which is the same as the control. No parasitism of the egg mass occurred after spraying with ei-ther AttackTM or OrtheneTM. The percentage of parasitised LCM eggs after Bt treatment was 13.48; the control showed the highest parasitism of LCM eggs (58.13 %). The mortality of T. chilo-nis adults (in descending order) due to the insecticides after 15 hours was Entrust, Attack, Orthene and Bt. The result suggests that Bt could be included in Integrated Pest Management Programmes that depend on T. chilonis parasitism of LCM eggs and T. chilonis activity

Pathway databases and tools for their exploitation: benefits, current limitations and challenges

In past years, comprehensive representations of cell signalling pathways have been developed by manual curation from literature, which requires huge effort and would benefit from information stored in databases and from automatic retrieval and integration methods. Once a reconstruction of the network of interactions is achieved, analysis of its structural features and its dynamic behaviour can take place. Mathematical modelling techniques are used to simulate the complex behaviour of cell signalling networks, which ultimately sheds light on the mechanisms leading to complex diseases or helps in the identification of drug targets. A variety of databases containing information on cell signalling pathways have been developed in conjunction with methodologies to access and analyse the data. In principle, the scenario is prepared to make the most of this information for the analysis of the dynamics of signalling pathways. However, are the knowledge repositories of signalling pathways ready to realize the systems biology promise? In this article we aim to initiate this discussion and to provide some insights on this issue

An immunohistochemical assessment of cellular proliferation markers in head and neck squamous cell cancers.

Prognostic information is essential for the evaluation, judgement and optimal treatment of patients with squamous cell cancers (SCCs) of the upper aerodigestive tract. Using immunohistochemical and flow cytometric techniques, we have studied the significance of cellular expression of the Ki-67 antigen, epidermal growth factor receptor (EGFR), the transferrin receptor (TFR) and DNA ploidy status in a prospective analysis of patients with SCCs of the head and neck region. All 42 fresh tumour samples (five well differentiated; 28 moderately differentiated; nine poorly differentiated) expressed both EGFR and TFR to varying degrees. Receptor expression was most marked on the peripheral invading margin of cancer cell islands although staining was also demonstrated in a random fashion within cellular islands and consistently along the basal cell layer of overlying stratified squamous epithelium. The percentage of cancer cells that reacted with the Ki-67 monoclonal antibody was assessed as low (less than 10%) in 15 samples (35.8%), intermediate (10-30%) in 19 samples (45.2%) and high (greater than 30%) in eight samples (19.0%). Eleven of 15 samples (73%) with a low percentage reactivity were DNA diploid, whereas seven of eight samples (87.5%) with a high percentage reactivity were DNA aneuploid. Poorly differentiated SCCs were significantly more often aneuploid than were either moderately or well differentiated tumours. Our results suggest that EGFR and TFR are widely distributed on SCCs, especially on proliferating cells at the invading tumour margin. In addition, there is a close spatial correlation between cells expressing EGFR, TFR and those expressing the Ki-67 antigen. Tumours in which the staining intensity for both EGFR and TFR was intense invariably expressed the Ki-67 antigen in a high proportion of cells. Further patient follow-up will be important in determining whether intense EGFR and TFR staining, combined with a high percentage reactivity with Ki-67 antibody and DNA aneuploidy, will ultimately define a subset of head and neck cancer patients with a poor clinical outcome

Parental financial support : A safety net for young adults with debt problems

Parental financial support benefits young adults in societies with decreasing welfare-state support and a pattern of early home-leaving. This article focuses on the association between young adults' debt problems and parental financial support: the extent to which indebted young adults receive financial help from their parents. We also investigate the extent to which specific benefits are associated with debt problems or parental financial support. The data were gathered in an online survey conducted among 18-to-35-year-old Finns (n = 1,019). The results revealed, first, that many parents safeguard their indebted adult children's lives by means of financial support and second, that heavy cash-welfare-benefit users are particularly likely to receive parental financial support. Our analysis also revealed that the prevalence of debt problems as well as of parental financial support were especially high among those who had received social assistance, sickness benefit or labour-market subsidy within the previous 12 months. In a society open to new social risks as well as to debt problems, young people who lack financial support from their parents have a rockier transition to adulthood than those who receive support.Peer reviewe

Structural and biochemical characterization of Chlamydia trachomatis DsbA reveals a cysteine-rich and weakly oxidising oxidoreductase

Copyright © 2016 Christensen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The Gram negative bacteria Chlamydia trachomatis is an obligate intracellular human pathogen that can cause pelvic inflammatory disease, infertility and blinding trachoma. C. trachomatis encodes a homolog of the dithiol oxidoreductase DsbA. Bacterial DsbA proteins introduce disulfide bonds to folding proteins providing structural bracing for secreted virulence factors, consequently these proteins are potential targets for antimicrobial drugs. Despite sharing functional and structural characteristics, the DsbA enzymes studied to date vary widely in their redox character. In this study we show that the truncated soluble form of the predicted membrane anchored protein C. trachomatis DsbA (CtDsbA) has oxidase activity and redox properties broadly similar to other characterized DsbA proteins. However CtDsbA is distinguished from other DsbAs by having six cysteines, including a second disulfide bond, and an unusual dipeptide sequence in its catalytic motif (Cys-Ser-Ala-Cys). We report the 2.7 Å crystal structure of CtDsbA revealing a typical DsbA fold, which is most similar to that of DsbA-II type proteins. Consistent with this, the catalytic surface of CtDsbA is negatively charged and lacks the hydrophobic groove found in EcDsbA and DsbAs from other enterobacteriaceae. Biochemical characterization of CtDsbA reveals it to be weakly oxidizing compared to other DsbAs and with only a mildly destabilizing active site disulfide bond. Analysis of the crystal structure suggests that this redox character is consistent with a lack of contributing factors to stabilize the active site nucleophilic thiolate relative to more oxidizing DsbA proteins