1,831 research outputs found
Probing the Reaction Mechanism of the D-ala-D-ala Dipeptidase, VanX, by Using Stopped-Flow Kinetic and Rapid-Freeze Quench EPR Studies on the Co(II)-Substituted Enzyme
In an effort to probe the reaction mechanism of VanX, the D-ala-D-ala dipeptidase required for high-level vancomycin resistance in bacteria, stopped-flow kinetic and rapid-freeze quench EPR studies were conducted on the Co(II)-substituted enzyme when reacted with d-ala-d-ala. The intensity of the Co(II) ligand field band at 550 nm decreased (ε550 = 140 to 18 M-1 cm-1) when VanX was reacted with substrate, suggesting that the coordination number of the metal increases from 5 to 6 upon substrate binding. The stopped-flow trace was fitted to a kinetic mechanism that suggests the presence of an intermediate whose breakdown is rate-limiting. Rapid-freeze quench EPR studies verified the presence of a reaction intermediate that exhibits an unusually low hyperfine constant (33 G), which suggests a bidentate coordination of the intermediate to the metal center. The EPR studies also identified a distinct enzyme product complex. The results were used to offer a detailed reaction mechanism for VanX that can be used to guide future inhibitor design efforts
College students’ perceptions regarding sensory aspects of conventionally produced and unconventionally produced foods: implications for marketing to the Millennial generation
Consumers vote every day on which products line the shelves of grocery stores, co-ops, and niche markets. Public unrest with regard to the environmental, animal welfare, food purity, and human health impacts of agricultural production practices have led to the rise of unconventionally produced (UP) food products. While the sales of UP foods is increasing, studies regarding the qualities of such products that impact consumer purchases have yielded inconsistent results. This study examined students’ perceptions of sensory aspects of conventionally produced (CP) and UP foods to better understand how sensory aspects impact decisions to purchase. Students reported consistent perceptions regarding the favorability of each sensory aspect of chicken and apples; the UP versions of the products yielded higher mean scores on every sensory aspect. However, students’ perceptions of the sensory qualities of chocolate, milk, and beef were not consistent; for example, they reported more favorable perceptions of the appearance and smell of CP milk, but perceived a more favorable texture and flavor from the UP milk. The results of this study imply that when making purchasing decisions, consumers may value specific sensory attributes over others. One approach to marketing UP products is to focus on valued extrinsic aspects designed to attract consumers to purchase products even though they may have less favorable perceptions of certain sensory qualities
Arctic and subarctic environmental analyses utilizing ERTS-1 imagery
The author has identified the following significant results. ERTS-1 imagery provides a means of distinguishing and monitoring estuarine surface water circulation patterns and changes in the relative sediment load of discharging rivers on a regional basis. Physical boundaries mapped from ERTS-1 imagery in combination with ground truth obtained from existing small scale maps and other sources resulted in improved and more detailed maps of permafrost terrain and vegetation for the same area. Snowpack cover within a research watershed has been analyzed and compared to ground data. Large river icings along the proposed Alaska pipeline route from Prudhoe Bay to the Brooks Range have been monitored. Sea ice deformation and drift northeast of Point Barrow, Alaska have been measured during a four day period in March and shore-fast ice accumulation and ablation along the west coast of Alaska have been mapped for the spring and early summer seasons
A Five-coordinate Metal Center in Co(II)-substituted VanX
In an effort to structurally probe the metal binding site in VanX, electronic absorption, EPR, and extended x-ray absorption fine structure (EXAFS) spectroscopic studies were conducted on Co(II)-substituted VanX. Electronic spectroscopy revealed the presence of Co(II) ligand field transitions that had molar absorptivities of ∼100 m–1 cm–1, which suggests that Co(II) is five-coordinate in Co(II)-substituted VanX. Low temperature EPR spectra of Co(II)-substituted VanX were simulated using spin Hamiltonian parameters of M = |±½〉, E/D = 0.14, greal(x,y) = 2.37, and grealS(z) = 2.03. These parameters lead to the prediction that Co(II) in the enzyme is five-coordinate and that there may be at least one solvent-derived ligand. Single scattering fits of EXAFS data indicate that the metal ions in both native Zn(II)-containing and Co(II)-substituted VanX have the same coordination number and that the metal ions are coordinated by 5 nitrogen/oxygen ligands at ∼ 2.0 Å. These data demonstrate that Co(II) (and Zn(II) from EXAFS studies) is five-coordinate in VanX in contrast to previous crystallographic studies (Bussiere, D. E., Pratt, S. D., Katz, L., Severin, J. M., Holzman, T., and Park, C. H. (1998) Mol. Cell 2, 75–84). These spectroscopic studies also demonstrate that the metal ion in Co(II)-substituted VanX when complexed with a phosphinate analog of substrate d-Ala-d-Ala is also five-coordinate
Mano a Mano Online Information System: A Technical Report
From Morse Code, to the rotary telephone, to the internet, communication among individuals has been and still is the champion for human growth and development. Moreover, human communication is key for educational, professional, and personal development. Mano a Mano (Spanish for "Hand to Hand"), an online system designed for UNC-CH students, staff, faculty and organizations to meet, greet, and achieve serves a vital role in the educational, professional, and personal development of such campus affiliates. In addition, it strives to weaken the campus population barrier to communication by providing a means in which campus affiliates can make contacts among a diverse body of individuals. Designed in accordance with the conceptual framework of the iterative method proposed by Mullen and Nigay (1996), Mano a Mano and its underlying objectives intend to set UNC-CH apart from other UNC system colleges and universities by introducing a novel, technological platform of campus communication
Ionic partitioning of KCl in AOT reverse micelles from molecular dynamics simulations
Reverse micelles are an important class of nanoreactors providing an array of applications. The structure and dynamics of aqueous reverse micelles have been intensely investigated, yet there are many subtleties involved in characterizing the arrangement of water and ionic species within these aggregates. The interfacial arrangement of water and dopants added to bis(2-ethylhexyl) sulfosuccinate sodium salt (AOT) reverse micelles were investigated by fully atomistic molecular dynamics simulations. Reverse micelles of increasing water to surfactant ratio were doped with concentrations of KCl from 0.0 to 1.0 M to analyze the partitioning of ions and water. Detailed analysis of pair density functions reveals that Cl− ions are embedded deep in the aqueous interior, and the K+ ions display a remarkable affinity for the interface. Our results confirm the existence of multiple layers of water, and an ordered multi-layer ionic arrangement that is driven by the negatively charged surfactant headgroup
Are We Missing Important Areas in Pelagic Marine Conservation? Redefining Conservation Hotspots in the Ocean
The protection of biodiversity is one of the most important goals in terrestrial and marine conservation. Marine conservation approaches have traditionally followed the example of terrestrial initiatives. However, patterns, processes, habitats, and threats differ greatly between the 2 systems - and even within the marine environment. As a result, there is still a lack of congruence as to how to best identify and prioritize conservation approaches moving from the static terrestrial and nearshore realm into a more fluid, 3-dimensional pelagic realm. To address this problem, we investigate how the conservation science literature has been used to inform and guide management strategies in the marine system from coastal to pelagic environments. As cumulative impacts on the health of the oceans continue to increase, conservation priorities have shifted to include highly dynamic areas of the pelagic marine system. By evaluating whether priorities match science with current place-based management approaches (i.e. marine protected areas, MPAs), we identify important gaps that must be considered in current conservation schemes. Effective pelagic MPA design requires monitoring and evaluation across multiple physical, biological, and human dimensions. Because many threatened and exploited marine species move through an ephemeral and ever-changing environment, our results highlight the need to move beyond traditional, 2-dimensional approaches to marine conservation, and into dynamic management approaches that incorporate metrics of biodiversity as well as oceanographic features known to promote multilevel, trophic productivity
Searching for Galactic White Dwarf Binaries in Mock LISA Data using an F-Statistic Template Bank
We describe an F-statistic search for continuous gravitational waves from
galactic white-dwarf binaries in simulated LISA Data. Our search method employs
a hierarchical template-grid based exploration of the parameter space. In the
first stage, candidate sources are identified in searches using different
simulated laser signal combinations (known as TDI variables). Since each source
generates a primary maximum near its true "Doppler parameters" (intrinsic
frequency and sky position) as well as numerous secondary maxima of the
F-statistic in Doppler parameter space, a search for multiple sources needs to
distinguish between true signals and secondary maxima associated with other,
"louder" signals. Our method does this by applying a coincidence test to reject
candidates which are not found at nearby parameter space positions in searches
using each of the three TDI variables. For signals surviving the coincidence
test, we perform a fully coherent search over a refined parameter grid to
provide an accurate parameter estimation for the final candidates. Suitably
tuned, the pipeline is able to extract 1989 true signals with only 5 false
alarms. The use of the rigid adiabatic approximation allows recovery of signal
parameters with errors comparable to statistical expectations, although there
is still some systematic excess with respect to statistical errors expected
from Gaussian noise. An experimental iterative pipeline with seven rounds of
signal subtraction and re-analysis of the residuals allows us to increase the
number of signals recovered to a total of 3419 with 29 false alarms.Comment: 29 pages, 11 figures; submitted to Classical and Quantum Gravit
LISA Data Analysis using MCMC methods
The Laser Interferometer Space Antenna (LISA) is expected to simultaneously
detect many thousands of low frequency gravitational wave signals. This
presents a data analysis challenge that is very different to the one
encountered in ground based gravitational wave astronomy. LISA data analysis
requires the identification of individual signals from a data stream containing
an unknown number of overlapping signals. Because of the signal overlaps, a
global fit to all the signals has to be performed in order to avoid biasing the
solution. However, performing such a global fit requires the exploration of an
enormous parameter space with a dimension upwards of 50,000. Markov Chain Monte
Carlo (MCMC) methods offer a very promising solution to the LISA data analysis
problem. MCMC algorithms are able to efficiently explore large parameter
spaces, simultaneously providing parameter estimates, error analyses and even
model selection. Here we present the first application of MCMC methods to
simulated LISA data and demonstrate the great potential of the MCMC approach.
Our implementation uses a generalized F-statistic to evaluate the likelihoods,
and simulated annealing to speed convergence of the Markov chains. As a final
step we super-cool the chains to extract maximum likelihood estimates, and
estimates of the Bayes factors for competing models. We find that the MCMC
approach is able to correctly identify the number of signals present, extract
the source parameters, and return error estimates consistent with Fisher
information matrix predictions.Comment: 14 pages, 7 figure
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