Efficiency assessment of trypanocidal treatments in the research station of Avetonou in Togo

In Togo as elsewhere, among the animal trypanosomosis control strategies, the chemotherapy constitutes the most used method. In order to assess the efficiency of two trypanocidal drugs (isometamidium chloride and diminazene) on cattle, a longitudinal study was conducted from September to November 2011 in the research station of Avetonou located in the Prefecture of Agou in Togo. A parasitological survey was carried out on two groups of 90 cattle each (for a total of 180 cattle) using the buffy coat technique at regular intervals of two weeks over a total period of 56 days. The animals of the first group were treated with isometamidium chloride (0.5 mg/kg) and the second group (untreated animals) was used as a control group. The comparison of the incidence of trypanosomosis between the two groups was performed using three statistical tests: the Chisquared test, the test of risk Reduction, and the “Eisler ratio test”. These analyses revealed an inefficiency of the preventive treatment with isometamidium chloride at the dose of 0.5 mg/kg bodyweight contrarily to the curative treatment that was effective. For diminazene, used at a dose of 3.5 mg/kg bodyweight, strains of Trypanosoma vivax and Trypanosoma congolense were not eliminated in 14.29% of cases.Keyswords: Trypanocidal treatment, isometamidium, diminazene, Avétonou, Togo

Post Eclosion Age Predicts the Prevalence of Midgut Trypanosome Infections in Glossina

The teneral phenomenon, as observed in Glossina sp., refers to the increased susceptibility of the fly to trypanosome infection when the first bloodmeal taken is trypanosome-infected. In recent years, the term teneral has gradually become synonymous with unfed, and thus fails to consider the age of the newly emerged fly at the time the first bloodmeal is taken. Furthermore, conflicting evidence exists of the effect of the age of the teneral fly post eclosion when it is given the infected first bloodmeal in determining the infection prevalence. This study demonstrates that it is not the feeding history of the fly but rather the age (hours after eclosion of the fly from the puparium) of the fly when it takes the first (infective) bloodmeal that determines the level of fly susceptibility to trypanosome infection. We examine this phenomenon in male and female flies from two distinct tsetse clades (Glossina morsitans morsitans and Glossina palpalis palpalis) infected with two salivarian trypanosome species, Trypanosoma (Trypanozoon) brucei brucei and Trypanosoma (Nannomonas) congolense using Fisher's exact test to examine differences in infection rates. Teneral tsetse aged less than 24 hours post-eclosion (h.p.e.) are twice as susceptible to trypanosome infection as flies aged 48 h.p.e. This trend is conserved across sex, vector clade and parasite species. The life cycle stage of the parasite fed to the fly (mammalian versus insect form trypanosomes) does not alter this age-related bias in infection. Reducing the numbers of parasites fed to 48 h.p.e., but not to 24 h.p.e. flies, increases teneral refractoriness. The importance of this phenomenon in disease biology in the field as well as the necessity of employing flies of consistent age in laboratory-based infection studies is discussed

A Multi-Host Agent-Based Model for a Zoonotic, Vector-Borne Disease. A Case Study on Trypanosomiasis in Eastern Province, Zambia

Background: This paper presents a new agent-based model (ABM) for investigating T. b. rhodesiense human African trypanosomiasis (rHAT) disease dynamics, produced to aid a greater understanding of disease transmission, and essential for development of appropriate mitigation strategies. Methods: The ABM was developed to model rHAT incidence at a fine spatial scale along a 75 km transect in the Luangwa Valley, Zambia. The method offers a complementary approach to traditional compartmentalised modelling techniques, permitting incorporation of fine scale demographic data such as ethnicity, age and gender into the simulation. Results: Through identification of possible spatial, demographic and behavioural characteristics which may have differing implications for rHAT risk in the region, the ABM produced output that could not be readily generated by other techniques. On average there were 1.99 (S.E. 0.245) human infections and 1.83 (S.E. 0.183) cattle infections per 6 month period. The model output identified that the approximate incidence rate (per 1000 person-years) was lower amongst cattle owning households (0.079, S.E. 0.017), than those without cattle (0.134, S.E. 0.017). Immigrant tribes (e.g. Bemba I.R. = 0.353, S.E.0.155) and school-age children (e.g. 5–10 year old I.R. = 0.239, S.E. 0.041) were the most at-risk for acquiring infection. These findings have the potential to aid the targeting of future mitigation strategies. Conclusion: ABMs provide an alternative way of thinking about HAT and NTDs more generally, offering a solution to the investigation of local-scale questions, and which generate results that can be easily disseminated to those affected. The ABM can be used as a tool for scenario testing at an appropriate spatial scale to allow the design of logistically feasible mitigation strategies suggested by model output. This is of particular importance where resources are limited and management strategies are often pushed to the local scale. © 2016 Alderton et al

Attenuation of the Sensing Capabilities of PhoQ in Transition to Obligate Insect–Bacterial Association

Sodalis glossinidius, a maternally inherited endosymbiont of the tsetse fly, maintains genes encoding homologues of the PhoP-PhoQ two-component regulatory system. This two-component system has been extensively studied in facultative bacterial pathogens and is known to serve as an environmental magnesium sensor and a regulator of key virulence determinants. In the current study, we show that the inactivation of the response regulator, phoP, renders S. glossinidius sensitive to insect derived cationic antimicrobial peptides (AMPs). The resulting mutant strain displays reduced expression of genes involved in the structural modification of lipid A that facilitates resistance to AMPs. In addition, the inactivation of phoP alters the expression of type-III secretion system (TTSS) genes encoded within three distinct chromosomal regions, indicating that PhoP-PhoQ also serves as a master regulator of TTSS gene expression. In the absence of phoP, S. glossinidius is unable to superinfect either its natural tsetse fly host or a closely related hippoboscid louse fly. Furthermore, we show that the S. glossinidius PhoQ sensor kinase has undergone functional adaptations that result in a substantially diminished ability to sense ancestral signals. The loss of PhoQ's sensory capability is predicted to represent a novel adaptation to the static symbiotic lifestyle, allowing S. glossinidius to constitutively express genes that facilitate resistance to host derived AMPs

Transmissibility, by Glossina morsitans morsitans, of Trypanosoma congolense strains during the acute and chronic phases of infection

In order to verify whether chronic trypanosomal infections can affect the transmissibility of Trypanosoma congolense by tsetse flies, batches of Glossina morsitans morsitans were fed on mice infected with the same level of parasitemia (10(8.1)trypanosomes/ml of blood) of two cloned low virulent T. congolense strains during the acute and the chronic phases of infection. Results showed that the proportions of procyclic infections in flies that were fed during the acute phase (32.6% and 45.4% for isolates 1 and 2, respectively) were significantly higher (chi(2)=4.7, P0.05). The results of this study suggest that T. congolense loses part of its transmissiblity by tsetse flies during the chronic phase of infection. Copyright 2009. Published by Elsevier B.V

The transmissibility of Trypanosoma congolense seems to be associated with its level of resistance to isometamidium chloride

In large parts of Africa the control of livestock trypanosomiasis relies on the use of trypanocidal drugs. Resistance against the available compounds is developing rapidly in the trypanosome population. The effect of the development of drug resistance on the fitness of the trypanosome is not well known. To determine the effect of the development of resistance to isometamidium chloride on the trypanosome's transmissibility, transmission experiments were conducted. Use was made of three isogenic clones of Trypanosoma congolense with different susceptibility to the drug. The infection rate in Glossina morsitans morsitans differed significantly between clones and was significantly higher in tsetse flies infected with the T. congolense clone with the highest level of drug resistance

Dynamics of the corrosion for SAC305 solder alloy in salt environment

This study presents the corrosion dynamics of SAC305 solder alloy in two configurations (BGA solder balls components and solder material in globule) using a neutral salt spray test (NSS). SAC305 solder alloys were aged in different exposure durations ranging from 24 to 96 hours in a salt spray chamber with one salinity of 5 % NaCl at five temperatures ranging from 25°C to 45°C. Corrosion morphology, chemical composition and distribution of the elements in the corroded area and the corrosion product phases produced respectively after OM, SEM, EDX and XRD were also analyzed. The visible corrosion criterion defined on the OM and SEM results showed that the corroded areas are visible after 72h at 25°C, 60h at 30°C, 48h at 35°C, 36h at 40°C and only 24 h, at 45°C in case of BGAs solder balls components. The corroded area becomes larger at 96 hours. This indicates that temperature accelerates the corrosion mechanism. Moreover, according to the EDX result, tin (Sn), oxygen (O), and chloride ion (Cl-) were detected in the corroded area on solder balls. An activation energy (Ea) of 0.81 eV has been calculated. XRD measurements confirmed that a complex oxide chloride hydroxide (Sn3O(OH)2Cl2) was formed as a corrosion product on SAC globules. Finally, the kinetics of the failure mechanism of corrosion was investigated to refine the dynamic models of the degradation and overlay it on excessive stresses by simulations in order to predict lead-free solder alloys' lifetime

Investigations on the transmissibility of Trypanosoma congolense by the tsetse fly Glossina morsitans morsitans during its development in mammalian host

Experiments were conducted to investigate the effect of the developmental stage of a monomorphic T. congolense IL1180 strain, in a vertebrate host, on its transmissibility by the tsetse fly Glossina morsitans morsitans Westwood (Diptera: Glossinidae). Batches of 160 male teneral tsetse flies were given a single bloodmeal on mice infected with this T. congolense strain 4, 5, 6, 7 or 10 days post-infection. The proportion of infected flies in each of those batches showed that the stage of development of the trypanosome does affect the proportion of flies that develop a mature or immature infection with immature and mature infection rates of flies infected on days 5 or 10 significantly higher. The proportion of infected flies was not affected by the parasitaemia at the moment of infection. Results show that tsetse flies can become infected at any phase of the development of the T. congolense IL 1180 strain but the ease with which trypanosomes develop in the fly depends on the phase in the parasite's development in the host. Those observations suggest that in analogy with the pleomorphic T. brucei s.l. adaptation of the monomorphic T. congolense to development in the fly may also determine the parasite's transmissibility. Moreover, the findings stress the importance of standardising experiments in which the vectorial capacity of tsetse flies is determined and compared

Nutritional stress of adult female tsetse flies (Diptera: Glossinidae) affects the susceptibility of their offspring to trypanosomal infections

Tsetse-transmitted trypanosomiasis poses a serious threat to human and animal health in sub-Saharan Africa. The majority of tsetse flies (Glossina spp.) in a natural population will not develop a mature infection of either Trypanosoma congolense or Trypanosoma brucei sp. because of refractoriness, a phenomenon that is affected by different factors, including the tsetse fly's immune defence. Starvation of tsetse flies significantly increases their susceptibility to the establishment of a trypanosome infection. This paper reports the effects of nutritional stress (starvation) on (a) uninduced baseline levels of gene expression of the antimicrobial peptides attacin, defensin and cecropin in the tsetse fly, and (b) levels of expression induced in response to bacterial (Escherichia coli) or trypanosomal challenge. In newly emerged, unfed tsetse flies, starvation significantly lowers baseline levels of antimicrobial peptide gene expression, especially for attacin and cecropin. In response to trypanosome challenge, only non-starved older flies showed a significant increase in antimicrobial peptide gene expression within 5 days of ingestion of a trypanosome-containing bloodmeal, especially with T. brucei bloodstream forms. These data suggest that a decreased expression of immune genes in newly hatched flies or a lack of immune responsiveness to trypanosomes in older flies, both occurring as a result of fly starvation, may be among the factors contributing to the increased susceptibility of nutritionally stressed tsetse flies to trypanosome infection