Analyse de quelques aspects du système de production légumière et perception des producteurs de l’utilisation d’extraits botaniques dans la gestion des insectes ravageurs des cultures maraîchères au Sud du Togo

L’une des contraintes liée à la production maraîchère, est la pression exercée par les insectes ravageurs. Cette étude analyse quelques aspects du système de production légumière ainsi que la perception des   producteurs de l’utilisation d’extraits botaniques dans la gestion des  insectes ravageurs des cultures maraîchères. Elle a été réalisée à travers une enquête menée auprès de 217 maraîchers au Sud du Togo. L’analyse descriptive des données a révélé que plus d’hommes (72%) que de femmes (28%) pratiquent le maraîchage. Seul 33% a suivi une fois une formation en maraîchage. La contrainte majeure dans la production maraîchère est le problème de ravageurs et de maladies dont les principaux sont des insectes, des nématodes et des champignons. La lutte chimique constitue la principale méthode de lutte contre ces ravageurs. Néanmoins, les maraîchers connaissent des dangers induits par l’utilisation des pesticides de synthèse et sont au courant de l’utilisation des pesticides botaniques en maraîchage. Cependant, très peu les utilisent dans la gestion des ravageurs. Mais, ils sont disposés à utiliser les pesticides botaniques si ceux-ci sont disponibles, accessibles, efficaces et pas trop chers pour pallier les effets néfastes des pesticides de synthèse à la santé et à l’environnement.Mots clés : Maraîchage, perception des producteurs, extraits botaniques, Sud du Togo

Selection of sites for the in situ conservation of four traditional leafy vegetables consumed in Benin

Acmella uliginosa, Ceratotheca sesamoides, Justicia tenella and Sesamum radiatum are four traditional leafy vegetables which are widely consumed in Benin. In order to document their origin, folk nomenclature, geographical distribution and ecology and to select adequate zones for in situ conservation of their genetic resources, 118 villages were randomly selected and surveyed throughout the country using a participatory research approach. All the species apart from Ceratotheca sesamoides were reported by farmers as introduced in Benin at different periods. The folk nomenclature comprising 93 different names is based on nine key criteria of variable frequencies according to the species. The geographical distribution maps revealed that the species under study are unequally distributed. Indeed, A. uliginosa is found exclusively in the northwest, J. tenella in the northeast and partially in the centre while S. radiatum and Ceratotheca sesamoides were found almost everywhere in Benin. Results from multivariate analysis combined with geographical distribution maps of the species and the soil and climate maps of Benin, leaded to the selection of the Northwest and of the Northeast regions as the appropriate conservation zones for the four species. Molecular analysis was recommended as it can help in describing genetic diversity and consequently in identifying additional sites for the widely spread species Sesamum radiatum and Ceratotheca sesamoides. Keywords: Folk nomenclature, biodiversity, geographical distribution, site selection, in situ conservation, Benin

Applications and Action Mechanisms of Probiotic-Based Multi-Components

editorial reviewedProbiotic-based multi-component preparations refer to a mixture of bioactive agents, containing probiotics or postbiotics as main functional ingredients, and prebiotics, protectants, stabilizers, encapsulating agents, and other compounds as additional constituents.One Health approaches to support agroecological transformation of peri-urban farming1. No poverty2. Zero hunger3. Good health and well-being10. Reduced inequalities11. Sustainable cities and communities12. Responsible consumption and production13. Climate action17. Partnerships for the goal

Genetic characterization of two traditional leafy vegetables (Sesamum radiatum Thonn. ex Hornem and Ceratotheca sesamoides Endl.) of Benin, using flow cytometry and amplified fragment length polymorphism (AFLP) markers

AGAP : Equipe AFEF ‘Architecture et Fonctionnement des Espèces fruitières’ ; Team AFFS ‘Architecture and Functioning of Fruit Species’ Contact : [email protected] audienceAmplified fragment length polymorphism (AFLP) markers and flow cytometry were applied for the genetic characterization of wild and cultivated accessions of Sesamum radiatum and Cerathoteca sesamoides; two neglected and underutilized species of traditional leafy vegetable consumed in Benin. The average 2C nuclear DNA content per nucleus was found to be 1.99 +/- 0.06 and 1.05 +/- 0.06 pg for S. radiatum and C. sesamoides, respectively which correspond to estimated genome size of 1946,22 Mpb for S. radiatum and 1026,9 Mpb for C. sesamoides. No variation in DNA content could be detected within accessions from each analysed species. Also, no relation was found between nuclear DNA content, ecogeographical origin and the status (cultivated or wild) of the analyzed accessions. AFLP markers revealed low diversity within the accessions analyzed. Results from the study contributed to a better characterization of S. radiatum and C. sesamoides accessions and will help in defining both genetic resources conservation and breeding strategies

Assessment of genetic diversity among accessions of two traditional leafy vegetables (Acmella uliginosa (L.) and Justicia tenella (Nees) T.) consumed in Benin using amplified fragment length polymorphism (AFLP) markers

Amplified fragment length polymorphism (AFLP) markers were used to evaluate the genetic diversity and explore the genetic relationship among accessions of Acmella uliginosa and Justicia tenella, two leaf vegetables collected from different areas in the northwest and northeast parts of Benin (West Africa). The total number of exploitable amplicons revealed with genomic DNA from A. uliginosa was 224 with an average of 50.5% polymorphic amplicons. Using DNA from J. tenella, we obtained 34% of polymorphic amplicons from a total of 418. The coefficient of dissimilarity varied from 0.01 to 0.67 and from 0.17 to 0.62 for Acmella and Justicia, respectively. Low genetic diversity was observed among Acmella accessions although three distinct clusters could be differentiated. Contrarily, a great genetic diversity was observed among J. tenella accessions. In addition to this, most of the clusters were heterogeneous and showed the relationship between accessions collected from northeast and northwest. Our results confirm the robustness of AFLP techniques for genetic diversity studies and they provide the first set of molecular data for these two species.Key words: Amplified fragment length polymorphism (AFLP), genetic diversity, leafy vegetable, Benin

Assessment of genetic diversity among accessions of two traditional leafy vegetables (Acmella uliginosa (L.) and Justicia tenella (Nees) T.) consumed in Benin using amplified fragment length polymorphism (AFLP) markers

Amplified fragment length polymorphism (AFLP) markers were used to evaluate the genetic diversity and explore the genetic relationship among accessions of Acmella uliginosa and Justicia tenella, two leaf vegetables collected from different areas in the northwest and northeast parts of Benin (West Africa). The total number of exploitable amplicons revealed with genomic DNA from A. uliginosa was 224 with an average of 50.5% polymorphic amplicons. Using DNA from J. tenella, we obtained 34% of polymorphic amplicons from a total of 418. The coefficient of dissimilarity varied from 0.01 to 0.67 and from 0.17 to 0.62 for Acmella and Justicia, respectively. Low genetic diversity was observed among Acmella accessions although three distinct clusters could be differentiated. Contrarily, a great genetic diversity was observed among J. tenella accessions. In addition to this, most of the clusters were heterogeneous and showed the relationship between accessions collected from northeast and northwest. Our results confirm the robustness of AFLP techniques for genetic diversity studies and they provide the first set of molecular data for these two species

Applications of Probiotic-Based Multi-Components to Human, Animal and Ecosystem Health: Concepts, Methodologies, and Action Mechanisms

Probiotics and related preparations, including synbiotics and postbiotics, are living and non-living microbial-based multi-components, which are now among the most popular bioactive agents. Such interests mainly arise from the wide range and numerous beneficial effects of their use for various hosts. The current minireview article attempts to provide an overview and discuss in a holistic way the concepts, methodologies, action mechanisms, and applications of probiotic-based multi-components in human, animal, plant, soil, and environment health. Probiotic-based multi-component preparations refer to a mixture of bioactive agents, containing probiotics or postbiotics as main functional ingredients, and prebiotics, protectants, stabilizers, encapsulating agents, and other compounds as additional constituents. Analyzing, characterizing, and monitoring over time the traceability, performance, and stability of such multi-component ingredients require relevant and sensitive analytical tools and methodologies. Two innovative profiling and monitoring methods, the thermophysical fingerprinting thermogravimetry–differential scanning calorimetry technique (TGA-DSC) of the whole multi-component powder preparations, and the Advanced Testing for Genetic Composition (ATGC) strain analysis up to the subspecies level, are presented, illustrated, and discussed in this review to respond to those requirements. Finally, the paper deals with some selected applications of probiotic-based multi-components to human, animal, plant, soil and environment health, while mentioning their possible action mechanisms