In vitro assessment of the recurrent doses of topical gaseous ozone in the removal of Enterococcus faecalis biofilms in root canals

Aim: To evaluate the potential antibacterial effect of recurrent doses of topical gaseous ozone on the Enterococcus faecalis biofilms growth in human root canals in vitro.Materials and Methods: One hundred and thirty four human single.rooted mandibular premolars were enlarged to a size 35 K.File. Each root canal were inoculated with an overnight culture of Enterococcus faecalis ATCC 29212 in tryptic soy broth for 24 hours and incubated for 7 days at 37‹C. At 7.day interval, 4 specimens were prepared for Scanning Electron Microscope (SEM) analysis to confirm the presence and purity of biofilms whilst the other contaminated root canals were irrigated and disinfected. One hundred root canals of total 134 specimens were selected to create the  experimental groups and divided into 5 subgroups. In each experimental group (n = 20) root canals), recurrent ozone doses were applied with different irrigation and disinfection protocols in 5 different time intervals. Bacterial growth was analyzed by counting viable E. faecalis on tryptic soy agar plates.Results: According to intergroup comparison results observed in the final sample collection analysis, the amount of remaining bacteria in the positive control group were found to be significantly higher compared to Groups 1, 2, 3, 4, 5 and the material control group (P < 0.01). The remaining amount of bacteria in the last count of Group 1 were found to be significantly higher compared to Group 2 (P < 0.05), Group 4 (P < 0.01), Group 5 (P < 0.05) and the material control group (P < 0.01).Conclusion: The application of topical gaseous ozone in recurrent doses provides a positive effect in the removal of E. faecalis biofilm from root canals. However, during disinfection procedure, the combined use of recurrent doses of topical gaseous ozone with 2% NaOCl enhanced its antibacterial effect against E. faecalis biofilm.Key words: Antibacterial effect, disinfection, Enterococcus faecalis biofilm, irrigation, recurrent doses, root canals, topical gaseous ozon

Contribution to the knowledge of the Pentatomidae (Heteroptera) fauna of Turkey

Külekçi, G., Yildirim, E., Tezcan, S. (2009): Contribution to the knowledge of the Pentatomidae (Heteroptera) fauna of Turkey. Linzer biologische Beiträge 41 (1): 697-70

A comparison of four different microleakage tests for assessment of leakage of root canal fillings

Objective. The purpose of this study was to compare four different microleakage tests (dye leakage, electrochemical test, bacterial test and fluid filtration) for evaluation of the coronal seal of teeth obturated using Thermafil or lateral condensation techniques

Oral health status and its relation with medication and dental fear in children with attention-deficit hyperactivity disorder

Objective: The objective of this study was to determine the ora-dental health and its relation with medication and dental fear in a group of Turkish children with attention-deficit hyperactivity disorder (ADHD).Subjects and Methods: The levels of dental fear of children were determined with The Dental Subscale of Children’s Fear Survey Schedule (CFSS-DS). The oral and dental health evaluation was performed. This study included a total of 117 children aged between 6 and 15 years and they were examined under two groups as “ADHD” (n = 59) and “Control” (n = 58). Ora-dental health variables were compared between the groups and were also analyzed in accordance with dental fear and medication.Results: ADHD children and the control group exhibited similar CFSS-DS scores (15–32). No significant differences existed in df(t)/df(s), DMF(T)/DMF(S), d/D values, and presence of the white spot lesions. ADHD children’s Mutans streptococci and Lactobacillus quantities were found significantly higher than the control group. The incidence of parafunctional habits of the ADHD children was also found high.Conclusions: ADHD children that were medicated exhibited similar dental caries prevalence and periodontal health status. Although ADHD group had similar dental-periodontal health status and dental fear level with the control group and using ADHD medicines did not make a significant effect on the ora-dental health parameters, the patients should be carefully followed up because they were categorized in high caries risk groups.Keywords: Attention‑deficit hyperactivity disorder, clinical study, dental fear, medication, ora‑dental healt