Application of reverse vaccinology for the identification of epitope candidates from Rickettsia rickettsii

Rocky mountain spotted fever is a severe disease caused by Rickettsia rickettsii that frequently causes the death of the patients. As there are not effective vaccines for this disease, we employed reverse vaccinology to find epitope candidates useful for vaccine development. To apply this bioinformatics, we used the following online software: ProPred1, RANKPEP, and HLA binding, to evaluate 143 amino acid sequences in the genome of Rickettsia rickettsii (NC_009882 Sheila Smith). This strategy allowed us to identify 19 epitope sequences with affinity to HLA I alleles: A0201, A24; HLA-B: B3501, B3901

Application of reverse vaccinology for the identification of epitope candidates from Rickettsia rickettsii

643-647Rocky mountain spotted fever is a severe disease caused by Rickettsia rickettsii that frequently causes the death of the patients. As there are not effective vaccines for this disease, we employed reverse vaccinology to find epitope candidates useful for vaccine development. To apply this bioinformatics, we used the following online software: ProPred1, RANKPEP, and HLA binding, to evaluate 143 amino acid sequences in the genome of Rickettsia rickettsii (NC_009882 Sheila Smith). This strategy allowed us to identify 19 epitope sequences with affinity to HLA I alleles: A0201, A24; HLA-B: B3501, B3901

Rickettsia typhi IN RODENTS AND R. felis IN FLEAS IN YUCATÁN AS A POSSIBLE CAUSAL AGENT OF UNDEFINED FEBRILE CASES

Rickettsia typhi is the causal agent of murine typhus; a worldwide zoonotic and vector-borne infectious disease, commonly associated with the presence of domestic and wild rodents. Human cases of murine typhus in the state of Yucatán are frequent. However, there is no evidence of the presence of Rickettsia typhi in mammals or vectors in Yucatán. The presence of Rickettsia in rodents and their ectoparasites was evaluated in a small municipality of Yucatán using the conventional polymerase chain reaction technique and sequencing. The study only identified the presence of Rickettsia typhi in blood samples obtained from Rattus rattus and it reported, for the first time, the presence of R. felis in the flea Polygenis odiosus collected from Ototylomys phyllotis rodent. Additionally, Rickettsia felis was detected in the ectoparasite Ctenocephalides felis fleas parasitizing the wild rodent Peromyscus yucatanicus. This study’s results contributed to a better knowledge of Rickettsia epidemiology in Yucatán

Indigenous participation in intercultural education: learning from Mexico and Tanzania. Ecology and Society

ABSTRACT. Intercultural education seeks to create a forum for integrating Western scientific knowledge and indigenous knowledge to address local and global challenges such as biocultural diversity conservation, natural resource management, and social justice for indigenous peoples. Intercultural education is based on learning together with, rather than learning about or from, indigenous communities. In the best examples, problem-based learning dissolves the dichotomy between indigenous and nonindigenous, resulting in full partnerships in which participants share expertise to meet mutual needs. With reference to literature and two illustrative examples of intercultural education initiatives in Mexico and Tanzania, we present an original conceptual framework for assessing indigenous participation in intercultural education. This incorporates a new ladder of participation depth (in relation to both curriculum content and decision making) alongside separate considerations of breadth, i.e., stakeholder diversity, and scope, i.e., the number of key project stages in which certain stakeholder groups are participating. The framework can be used to compare intercultural education initiatives in differing contexts and might be adaptable to other intercultural work

Rickettsia rickettsii isolation from naturally infected Amblyomma parvum ticks by centrifugation in a 24-well culture plate technique

Rocky Mountain spotted fever is an acute illness caused by Rickettsia rickettsii (R. rickettsii) and is  transmitted by the bite of ticks of the genera Dermacentor, Amblyomma and Rhipicephalus. The illness results  in a high mortality rate and may be easily confused with other febrile syndromes. In Yucatan State, Mexico, childhood cases with a high mortality have been reported. In this work we report the isolation of a  Mexican R. rickettsii strain from a tick egg mass using an alternative method for Rickettsia isolation with  24-well plates. We also identified a potential vector of R. rickettsii in the southeast of Mexico, which is  Amblyomma parvum.Keywords: Amblyomma parvum, Rickettsia rickettsii, Rocky Mountain spotted fever