Bridging the gap: scenario-based design as a solution for delayed access to users

Scenario-based design (Carroll & Rosson, 2002) is a Human-Computer Interaction methodology for considering the needs of potential users, without their direct input. Scenario-based design gives the interface designer the ability to create scenarios of use, along with postulations on the various types of users, expressed in the form of personas (Grudin & Pruitt, 2002). These scenarios and personas can be useful in the context of a design project, where real world issues preclude the direct involvement of users at a critical stage. By ‘walking through’ informal narrative descriptions in the form of a story, scenario-based design focuses on human activity rather than technology. We propose that scenario-based design can be utilised to fill gaps in a collaborative project, where, due to circumstances beyond the control of the designer, other parties have not provided vital information before the prototyping stage. Scenario-based design can allow us, as designers, to consider the needs of our potential users when circumstances mean that we would not otherwise be able to. While different disciplines utilise proprietary project management methods, scenario-based design bridges gaps between practice-specific epistemologies, allowing contributions from different fields to feed the project at any stage of its progress. This paper details our early reflections as designers working on an ARC Linking Grant-funded, interdisciplinary project to redesign a bushfire warning website. We explain that scenario-based design can act as a catalyst, to ‘kick-start’ a project where, due to various factors, timing or resources means that direct contact with users is not always possible

Fallas del estado, violencia y comercio: rutas comerciales peligrosas en Colombia

En este documento se investiga el efecto de la violencia armada doméstica provocada por la inestabilidad política sobre las fricciones de la distancia en la movilidad de carga. Además, el efecto de esas fricciones sobre el acceso diferencial de las regiones a los mercados globales. Se concluye que el sistema de transporte colombiano se ve obstaculizado por deficiencias en la infraestructura e instituciones de transporte terrestre y por entornos políticos fragmentados. El análisis a nivel micro de los registros de exportaciones hacia Estados Unidos corrobora que el envío de carga de exportación desde las regiones del interior se redirige para evitar exposiciones a la violencia armada doméstica a pesar de las amplias distancias de envío marítimo y terrestre existentes. Aprovechamos las trayectorias del transporte de carga desde las regiones colombianas y los patrones espaciales de los conflictos armados violentos para ver cómo los entornos geopolíticos inestables son perjudiciales para la movilidad del transporte de carga y la apertura del mercado. A través de un modelo de elección discreta se muestra que el flujo de envío se ve frenado por el desvío debido a la violencia armada doméstica y que las regiones del interior tienen acceso restringido al mercado global. La percepción de riesgo y comportamiento de redireccionamiento se considera heterogénea entre los envíos y está condicionada a las características del envío, como el tipo de mercancía, el tamaño y el valor del flete. Los resultados resaltan que el conseguir la estabilidad política puede ayudar a mejorar la movilidad de carga y el desarrollo económico orientado a la exportación en el Sur globalWe investigate the effect of domestic armed violence brought about by political instability on the geography of distance frictions in freight mobility and the resulting differential access of regions to global markets. The Colombian transportation system has been found to be impeded by deficiencies in landside transport infrastructure and institutions, and by fragmented political environments. The micro-level analysis of U.S.-bounded export shipping records corroborates that export freight shipping from inland regions is re-routed to avoid exposures to domestic armed violence despite greatly extended landside and maritime shipping distances. We exploit the trajectories of freight shipping from Colombian regions and spatial patterns of violent armed conflicts to see how unstable geopolitical environments are detrimental to freight shipping mobility and market openness. The discrete choice model shows that the shipping flow is greatly curbed by the extended re-routing due to domestic armed violence and that inland regions have restricted access to the global market. The perception of risk and re-routing behavior is found heterogeneous across shipments and conditional to shipment characteristics, such as commodity type, freight value and shipper sizes. The results highlight that political stability must be accommodated for improved freight mobility and export-oriented economic development in the global South.Fallas del estado, violencia y comercio: Rutas comerciales peligrosas en Colombia Enfoque En este artículo se investiga cómo el efecto la violencia armada, que surge en medio de entornos geopolíticos inestables, es perjudicial para la movilidad de carga y el acceso de las regiones a las cadenas globales de logística de mercancías. Nuestro análisis se centra en el caso de Colombia en 2006-2007, por ser un periodo que estuvo muy permeado por la violencia y delincuencia, en muchas de las rutas principales de transporte al interior del país. El objetivo es determinar cómo la inseguridad durante este período de tiempo actuó como un impedimento para el movimiento del transporte marítimo de carga en el comercio internacional, actuando como otros de sus determinantes, tales como los factores arancelarios y no arancelarios. De esta manera, se propone la hipótesis de que los encargados de la logística del transporte buscarían reducir su exposición a conflictos armados domésticos a lo largo de las rutas, desplazándose a puertos más lejanos, a los que se pueda acceder de manera más segura, pese a tener que opotar por distancias de envío más extendidas. Para este efecto, este trabajo explota la existencia de registros de embarque proveniente de las bases de datos de Port Import Export Reporting Services y los datos georreferenciados de conflictos armados nacionales de Uppsala Conflict Data Program. Contribución La visión tradicional en la literatura comercial se ha concentrado en cómo el efecto de los aranceles, las cuotas, el acceso al mar, las fronteras internacionales o la calidad de la infraestructura logística limitan las actividades de exportación y el comercio internacional. Nuestra principal contribución a la discusión está relacionada con los efectos que puede tener la inseguridad como otro impedimento importante para el cruce de fronteras de mercancías. Específicamente, calculamos las equivalencias en distancia del riesgo observado de conflictos armados internos para medir el costo del cambio de ruta y evaluamos hasta qué punto estos restringen el acceso a los mercados globales. Conclusiones Encontramos evidencia sobre Colombia, centrados en el período 2006-2007, de que el riesgo de violencia a lo largo de las rutas de transporte marítimo terrestre resulta en una geografía de la movilidad de carga alterada, con rutas de envío más riesgosas a los puertos que se evitan, en favor de otras opciones más seguras. Los transportistas cambiarían una ruta más corta, pero más riesgosa, por una ruta más segura a puertos ubicados más lejos del origen del envío. Además, estarían dispuestos a tomar la ruta de envío terrestre a puertos donde haya mayor seguridad, incluso en el caso en que el segmento marítimo de la distancia de envío, asociada al puerto de entrada de los Estados Unidos, sea más extenso. Nuestro estudio plantea que la falta de control de la violencia armada a lo largo de las rutas comerciales impide extensamente el transporte de carga, aumenta el costo de hacer negocios y limita en gran medida el acceso al mercado global, lo que podría desalentar las actividades económicas orientadas a la exportación. Frase destacada: Nuestro estudio plantea que la falta de control de la violencia armada a lo largo de las rutas comerciales impide extensamente el transporte de carga, aumenta el costo de hacer negocios y limita el acceso al mercado global, lo que podría desalentar las actividades económicas orientadas a la exportación

Soliton microcomb based spectral domain optical coherence tomography

Spectral domain optical coherence tomography (SD-OCT) is a widely used and minimally invaive technique for bio-medical imaging [1]. SD-OCT typically relies on the use of superluminescent diodes (SLD), which provide a low-noise and broadband optical spectrum. Recent advances in photonic chipscale frequency combs [2, 3] based on soliton formation in photonic integrated microresonators provide an chipscale alternative illumination scheme for SD-OCT. Yet to date, the use of such soliton microcombs in OCT has not yet been analyzed. Here we explore the use of soliton microcombs in spectral domain OCT and show that, by using photonic chipscale Si3N4 resonators in conjunction with 1300 nm pump lasers, spectral bandwidths exceeding those of commercial SLDs are possible. We demonstrate that the soliton states in microresonators exhibit a noise floor that is ca. 3 dB lower than for the SLD at identical power, but can exhibit significantly lower noise performance for powers at the milliWatt level. We perform SD-OCT imaging on an ex vivo fixed mouse brain tissue using the soliton microcomb, alongside an SLD for comparison, and demonstrate the principle viability of soliton based SD-OCT. Importantly, we demonstrate that classical amplitude noise of all soliton comb teeth are correlated, i.e. common mode, in contrast to SLD or incoherent microcomb states [4], which should, in theory, improve the image quality. Moreover, we demonstrate the potential for circular ranging, i.e. optical sub-sampling [5, 6], due to the high coherence and temporal periodicity of the soliton state. Taken together, our work indicates the promising properties of soliton microcombs for SD-OCT

Paraneoplastic Necrotizing Myopathy with a Mild Inflammatory Component: A Case Report and Review of the Literature

Inflammatory myopathies such as dermatomyositis and polymyositis are well-recognized paraneoplastic syndromes. Little is known, however, about necrotizing myopathies in association with cancer. We here describe a case of paraneoplastic necrotizing myopathy with a mild inflammatory infiltrate in a patient with adenocarcinoma. After the rapid development of a severe, disabling muscle weakness, the patient experienced near complete recovery within 4 months under oral prednisone treatment. In the context of the presented case, we will review current knowledge about paraneoplastic necrotizing myopathies

A beta-herpesvirus with fluorescent capsids to study transport in living cells.

Fluorescent tagging of viral particles by genetic means enables the study of virus dynamics in living cells. However, the study of beta-herpesvirus entry and morphogenesis by this method is currently limited. This is due to the lack of replication competent, capsid-tagged fluorescent viruses. Here, we report on viable recombinant MCMVs carrying ectopic insertions of the small capsid protein (SCP) fused to fluorescent proteins (FPs). The FPs were inserted into an internal position which allowed the production of viable, fluorescently labeled cytomegaloviruses, which replicated with wild type kinetics in cell culture. Fluorescent particles were readily detectable by several methods. Moreover, in a spread assay, labeled capsids accumulated around the nucleus of the newly infected cells without any detectable viral gene expression suggesting normal entry and particle trafficking. These recombinants were used to record particle dynamics by live-cell microscopy during MCMV egress with high spatial as well as temporal resolution. From the resulting tracks we obtained not only mean track velocities but also their mean square displacements and diffusion coefficients. With this key information, we were able to describe particle behavior at high detail and discriminate between particle tracks exhibiting directed movement and tracks in which particles exhibited free or anomalous diffusion

Erythropoietin Modulates the Structure of Bone Morphogenetic Protein 2–Engineered Cranial Bone

The ideally engineered bone should have similar structural and functional properties to the native tissue. Although structural integrity is critical for functional bone regeneration, we know less about modulating the structural properties of the engineered bone elicited by bone morphogenetic protein (BMP) than efficacy and safety. Erythropoietin (Epo), a primary erythropoietic hormone, has been used to augment blood transfusion in orthopedic surgery. However, the effects of Epo on bone regeneration are not well known. Here, we determined the role of Epo in BMP2-induced bone regeneration using a cranial defect model. Epo administration improved the quality of BMP2-induced bone and more closely resembled natural cranial bone with a higher bone volume (BV) fraction and lower marrow fraction when compared with BMP2 treatment alone. Epo increased red blood cells (RBCs) in peripheral blood and also increased hematopoietic and mesenchymal stem cell (MSC) populations in bone marrow. Consistent with our previous work, Epo increased osteoclastogenesis both in vitro and in vivo. Results from a metatarsal organ culture assay suggested that Epo-promoted osteoclastogenesis contributed to angiogenesis because angiogenesis was blunted when osteoclastogenesis was blocked by alendronate (ALN) or osteoprotegerin (OPG). Earlier calcification of BMP2-induced temporary chondroid tissue was observed in the Epo+BMP group compared to BMP2 alone. We conclude that Epo significantly enhanced the outcomes of BMP2-induced cranial bone regeneration in part through its actions on osteoclastogenesis and angiogenesis.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/98479/1/ten%2Etea%2E2011%2E0742.pd

Axigluon as Possible Explanation for ppˉ→ttˉp\bar{p} \to t\bar{t} Forward-Backward Asymmetry

A flavor-nonuniversal chiral color model is introduced. It is used for comparison to the recent data on $\bar{p} p \to \bar{t} t$. We concluded that the data are consistent with interpretation as an axigluon exchange within 1$\sigma$ and a unique rise and fall behavior is predicated with regard to the asymmetry $A^t_{FB}$ as a function of $t \bar{t}$ invariant mass, which can distinguish our model from others before one discovers the axigluon resonance. Further aspects of the model are discussed.Comment: reference update

A global map of terrestrial habitat types

Funder: NatureMap (https://naturemap.earth/) through Norway's International Climate and Forest Initiative (NICFI)Abstract: We provide a global, spatially explicit characterization of 47 terrestrial habitat types, as defined in the International Union for Conservation of Nature (IUCN) habitat classification scheme, which is widely used in ecological analyses, including for quantifying species’ Area of Habitat. We produced this novel habitat map for the year 2015 by creating a global decision tree that intersects the best currently available global data on land cover, climate and land use. We independently validated the map using occurrence data for 828 species of vertebrates (35152 point plus 8181 polygonal occurrences) and 6026 sampling sites. Across datasets and mapped classes we found on average a balanced accuracy of 0.77 (+¯0.14 SD) at Level 1 and 0.71 (+¯0.15 SD) at Level 2, while noting potential issues of using occurrence records for validation. The maps broaden our understanding of habitats globally, assist in constructing area of habitat refinements and are relevant for broad-scale ecological studies and future IUCN Red List assessments. Periodic updates are planned as better or more recent data becomes available

Complementation of hypersensitivity to DNA interstrand crosslinking agents demonstrates that XRCC2 is a Fanconi anaemia gene

Background Fanconi anemia (FA) is a heterogeneous inherited disorder clinically characterized by progressive bone marrow failure, congenital anomalies, and a predisposition to malignancies. Objective Determine, based on correction of cellular phenotypes, whether XRCC2 is a FA gene. Methods Cells (900677) from a previously identified patient with biallelic mutation of XRCC2, among other mutations, were genetically complemented with wild-type XRCC2. Results Wild-type XRCC2 corrects each of three phenotypes characteristic of FA cells, all related to the repair of DNA interstrand crosslinks, including increased sensitivity to mitomycin C (MMC), chromosome breakage, and G2-M accumulation in the cell cycle. Further, the p.R215X mutant of XRCC2, which is harbored by the patient, is unstable. This provides an explanation for the pathogenesis of this mutant, as does the fact that 900677 cells have reduced levels of other proteins in the XRCC2-RAD51B-C-D complex. Also, FANCD2 monoubiquitination and foci formation, but not assembly of RAD51 foci, are normal in 900677 cells. Thus, XRCC2 acts late in the FA-BRCA pathway as also suggested by hypersensitivity of 900677 cells to ionizing radiation. These cells also share milder sensitivities toward olaparib and formaldehyde with certain other FA cells. Conclusions XRCC2/FANCU is a FA gene, as is another RAD51 paralog gene, RAD51C/FANCO. Notably, similar to a subset of FA genes that act downstream of FANCD2, biallelic mutation of XRCC2/FANCU has not been associated with bone marrow failure. Taken together, our results yield important insights into phenotypes related to FA and its genetic origins

Greenhouse gas (CO2, CH4, and N2O) emissions after abandonment of agriculture

The GHG (CO2, CH4, N2O) emission potential along a chronosequence of former agricultural soils abandoned for 9 to 32 years were compared to an actively managed (on-going) agricultural soil (reference). The soils were incubated in mesocosms with and without manure amendment, and microbial functional groups involved in nitrous oxide emission were quantitatively assessed. Carbon dioxide emission significantly increased after agriculture abandonment (< 24 years) consistent with higher decomposition rate, but total emission decreased in the long term (> 29 years). With the cessation of agriculture, the abandoned sites generally became a net methane sink. Notably, total nitrous oxide emission showed a significant monotonic decrease over years of abandonment in response to manure amendment, possibly reflecting an altered capacity for (de)nitrification as indicated in the response of the (de)nitrifier abundance. Overall, our findings suggest that the GHG legacy of agriculture diminishes over time (> 29 years), with lowered GHG emissions and global warming potential (GWP) after abandonment of agriculture. © 2022, The Author(s)