Bentuk – Bentuk Partisipasi Warga Negara Dalam Pelestarian Lingkungan Hidup Berdasarkan Konsep Green Moral Di Kabupaten Blitar

Penelitian ini bertujuan untuk mengkaji dan menganalisa bagaimana bentuk bentuk partisipasi dalam pelestarian lingkungan di Kabupaten Blitar. Metodologi menggunakan pendekatan kualitatif. Hasil penelitian menunjukkan Bentuk-bentuk partisipasi Warga Negara dalam pelestarian lingkungan hidup dilakukan melalui kegiatan-kegiatan masyarakat dan dunia pendidikan (monolitik dan integratif). Kompetensi kewarganegaraan diperlukan agar warga Negara dapat berpartisipasi dalam lingkungan hidup dengan pembekalan dimensi/domain knowledge, skill dan disposition tentang lingkungan hidup melalui berbagai kegiatan di masyarakat dan pendidikan. Bentuk penguatan partisipasi warga Negara dalam Pelestarian Lingkungan Hidup dilakukan melalui kegiatan pelatihan bagi masyarakat, sekolah, sosialisasi tentang lingkungan hidup, penguatan peran organisasi-organisasi relawan lingkungan hidup, serta KMDM di SD dan sekolah SMP.Penguatan partisipasi warga negara tentang pelestarian lingkungan hidup berdasar konsep green moral dalam pembangunan berkelanjutan mengacu pada nilai-nilai Pancasila untuk sopan santun, bersih, mencintai lingkungan, dan menjaga lingkungan hidup untuk tercapai kepekaan terhadap lingkungan hidup melalui adaptasi dari hidup modern dengan mempertahankan dan melindungi lingkungan hidup, tujuan yang jelas dari pelestarian lingkungan hidup, integrasi dari nilai-nilai Pancasila dan lingkungan dalam integrasi berbagai macam kegiatan, serta adanya latency dalam sistem yang dibuat masyarakat dan pemerintah

Additional file 1: Table S1. of Machine learning classifier for identification of damaging missense mutations exclusive to human mitochondrial DNA-encoded polypeptides

MtDNA missense variants.1 dataset (mdmv.1). Each mutation is described by its polypeptide RefSeq code (Code), gene (GENE), amino acid position within the polypeptide (AA position), wild type amino acid (WT AA), mutant amino acid (M AA), domain (Domain) and phenotype of the mutation (Phenotype). Numerical scores and predicted phenotypes for the three tested predictors (Provean, Mutpred and PolyPhen-2) are also included. (XLS 608 kb

Additional file 13: Figure S4. of Machine learning classifier for identification of damaging missense mutations exclusive to human mitochondrial DNA-encoded polypeptides

Venn diagram showing unique and common damaging predictions among different predictors for amino acids substitutions with no clear evidences of pathogenicity. (DOC 93 kb

Severe Septic Patients with Mitochondrial DNA Haplogroup JT Show Higher Survival Rates: A Prospective, Multicenter, Observational Study

<div><p>Objective</p><p>In a previous cohort study (n=96), we found an association between mitochondrial (mt) DNA haplogroup JT and increased survival of severe septic patients, after controlling for age and serum lactic acid levels. The aim of this research was to increase the predictive accuracy and to control for more confounder variables in a larger cohort (n=196) of severe septic patients, to confirm whether mtDNA haplogroup JT influences short and medium-term survival in these patients.</p> <p>Methods</p><p>We conducted a prospective, multicenter, observational study in six Spanish Intensive Care Units. We determined 30-day and 6-month survival and mtDNA haplogroup in this second cohort of 196 patients and in the global cohort (first and second cohorts combined) with 292 severe septic patients. Multiple logistic regression and Cox regression analyses were used to test for the association of mtDNA haplogroups JT with survival at 30-days and 6-months, controlling for age, sex, serum interleukin-6 levels and SOFA score.</p> <p>Results</p><p>Logistic and Cox regression analyses showed no differences in 30-day and 6-month survival between patients with mtDNA haplogroup JT and other haplogroups in the first cohort (n=96). In the second cohort (n=196), these analyses showed a trend to higher 30-day and 6-month survival in those with haplogroup JT. In the global cohort (n=292), logistic and Cox regression analyses showed higher 30-day and 6-month survival for haplogroup JT. There were no significant differences between J and T sub-haplogroups in 30-day and 6-month survival.</p> <p>Conclusions</p><p>The global cohort study (first and second cohorts combined), the largest to date reporting on mtDNA haplogroups in septic patients, confirmed that haplogroup JT patients showed increased 30-day and 6-month survival. This finding may be due to single nucleotide polymorphism defining the whole haplogroup JT and not separately for J or T sub-haplogroups.</p> </div

Cumulative proportion of patients' survival at 30 days and 6 months according to mtDNA haplogroup JT versus no JT.

<p>Cumulative proportion of patients' survival at 30 days and 6 months according to mtDNA haplogroup JT versus no JT.</p

Mitochondrial DNA haplogroups.

<p>Haplogroup nomenclature is denoted in bold. N* defines those N non HV, JT or U individuals. HV* defines HV non H individuals. H* defines H non H1, H2 or H5 individuals. U5a* defines U5a non U5a1 individuals. U1811* defines U1811 non U4 or UK individuals. U* defines U non U4, UK, U1811* or U5 individuals. J1* defines J1 non J1c individuals. A) Haplogrouping strategy. The three numbers in brackets indicate the number of patients from each haplogroup and the number of one- and six-months survivors. B) Phylogeny summarizing the relationship between different mtDNA haplogroups.</p

Correlation analysis between GDF-15 and FGF-21.

<p>serum levels in samples from all patient groups taken as a whole (A), group 1(B), group 2 (C) and group 3(D).</p

Correlation analysis between GDF-15 and FGF-21 protein levels.

<p>in the conditioned medium of C2C12 cells treated with respiratory chain inhibitors.</p

Correlation analysis between GDF-15 and FGF-21.

<p>serum levels in samples from all patient groups taken as a whole (A), group 1(B), group 2 (C) and group 3(D).</p

Diagnostic performance of GDF-15 (A) and FGF-21 (B) for a cut-off value of 550pg/ml and 300pg/ml respectively.

<p>95% confidence intervals are indicated in brackets.</p