Study of the Activity of 3-benzyl-5-(4-chloro-arylazo)-4-thioxo-imidazolidin-2-one against Schistosomiasis Mansoni in Mice

Previous studies conducted with the imidazolidinic derivative 3-benzyl-5-(4-chloro-arylazo)-4-thioxo-imidazolidin-2-one (LPSF-PT05) show outstanding activity against adult Schistosoma mansoni worms in vitro. In the first phase of this study, S. mansoni-infected mice were treated, orally, with 100 mg/Kg of the LPSF-PT05 in three formulations: Tween 80 and saline solution, oil/water (70 : 30) emulsion, and solid dispersion with polyethylene glycol (PEG). In the second phase, three other doses of the LPSF-PT05 in PEG were tested: 3, 10, 30 mg/kg. These treatment regimens significantly reduced the number of recovered worms due to increases in the solubility of the compound in this formulation; the greatest reduction (70.5%) was observed at the dose of 100 mg/kg. There was no changes in the pattern of mature egg compared to immature eggs; however there was a significant increase in the number of dead eggs. Histopathological analysis of liver tissue showed changes in morphological aspects of the hepatic parenchyma with decrease exudative-productive hepatic granuloma stages, although we found no significant differences in IFN-γ, IL-4, IL-10, or NO production in response to the specific antigen SEA. The results show the derivative LPSF-PT05 to be a potential candidate in the etiological treatment of schistosomiasis with a possible dampening effect of the granulomatous process

Role of TNF-Alpha, IFN-Gamma, and IL-10 in the Development of Pulmonary Tuberculosis

Submitted by Kamylla Nascimento ([email protected]) on 2018-04-04T12:19:44Z No. of bitstreams: 1 Role of TNF-Alpha, IFN-Gamma, and IL-10 in the Development of.pdf: 902027 bytes, checksum: cd83bdb363de6b98d3ba1916cc523963 (MD5)Approved for entry into archive by Kamylla Nascimento ([email protected]) on 2018-04-04T13:51:31Z (GMT) No. of bitstreams: 1 Role of TNF-Alpha, IFN-Gamma, and IL-10 in the Development of.pdf: 902027 bytes, checksum: cd83bdb363de6b98d3ba1916cc523963 (MD5)Made available in DSpace on 2018-04-04T13:51:31Z (GMT). No. of bitstreams: 1 Role of TNF-Alpha, IFN-Gamma, and IL-10 in the Development of.pdf: 902027 bytes, checksum: cd83bdb363de6b98d3ba1916cc523963 (MD5) Previous issue date: 2012Universidade Federal Rural de Pernambuco. Departamento de Biologia. Recife, PE, Brasil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Universidade Federal de Pernanbuco (UFPE). Centro Acadêmico de Vitória. Núcleo de Educação Física e Ciências do Esporte. Vitória de Santo Antão, PE, Brazil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Host immune response against Mycobacterium tuberculosis is mediated by cellular immunity, in which cytokines and Th1 cells play a critical role. In the process of control of the infection by mycobacteria, TNF-alpha seems to have a primordial function. This cytokine acts in synergy with IFN-gamma, stimulating the production of reactive nitrogen intermediates (RNIs), thus mediating the tuberculostatic function of macrophages, and also stimulating the migration of immune cells to the infection site, contributing to granuloma formation, which controls the disease progression. IFN-gamma is the main cytokine involved in the immune response against mycobacteria, and its major function is the activation of macrophages, allowing them to exert its microbicidal role functions. Different from TNF-alpha and IFN-gamma, IL-10 is considered primarily an inhibitory cytokine, important to an adequate balance between inflammatory and immunopathologic responses. The increase in IL-10 levels seems to support the survival of mycobacteria in the host. Although there is not yet conclusive studies concerning a clear dichotomy between Th1 and Th2 responses, involving protective immunity and susceptibility to the disease, respectively, we can suggest that the knowledge about this responses based on the prevailing cytokine profile can help to elucidate the immune response related to the protection against M. tuberculosis

Evaluation of Antioxidant, Immunomodulatory, and Cytotoxic Action of Fractions from Eugenia uniflora

An increasing number of biological activities presented by medicinal plants has been investigated over the years, and they are used in the search for new substances with lower side effects. Eugenia uniflora L. and Eugenia malaccensis L. (Myrtaceae) have many folk uses in various countries. This current study was designed to quantify the polyphenols and flavonoids contents and evaluate the immunomodulatory, antioxidant, and cytotoxic potentials of fractions from E. uniflora L. and E. malaccensis L. It was observed that the polyphenol content was higher in ethyl acetate fractions. These fractions have high antioxidant potential. E. malaccensis L. seeds showed the largest DPPH radical scavenger capacity (EC50=22.62). The fractions of E. malaccensis L. leaves showed lower antioxidant capacity. The samples did not alter the profile of proinflammatory cytokines and nitric oxide release. The results indicate that species of the family Myrtaceae are rich in compounds with antioxidant capacity, which can help reduce the inflammatory response

Immunological studies and in vitro schistosomicide action of new imidazolidine derivatives

Submitted by Kamylla Nascimento ([email protected]) on 2018-09-13T12:01:52Z No. of bitstreams: 1 Immunological studies and in vitro schistosomicide action of new.pdf: 513006 bytes, checksum: 99dae120bb5aaddeedc985b1a17d2914 (MD5)Approved for entry into archive by Kamylla Nascimento ([email protected]) on 2018-09-13T12:27:26Z (GMT) No. of bitstreams: 1 Immunological studies and in vitro schistosomicide action of new.pdf: 513006 bytes, checksum: 99dae120bb5aaddeedc985b1a17d2914 (MD5)Made available in DSpace on 2018-09-13T12:27:26Z (GMT). No. of bitstreams: 1 Immunological studies and in vitro schistosomicide action of new.pdf: 513006 bytes, checksum: 99dae120bb5aaddeedc985b1a17d2914 (MD5) Previous issue date: 2011Fundação Federal de Pesquisa e Desenvolvimento (FINEP), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) e Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de Pernambuco (UFPE). Departamento de Antibióticos. Laboratório de Desenho e Síntese de Fármacos. Recife, PE, Brasil.Universidade Federal de Pernambuco (UFPE). Departamento de Antibióticos. Laboratório de Desenho e Síntese de Fármacos. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Universidade Federal de Pernambuco (UFPE). Departamento de Antibióticos. Laboratório de Desenho e Síntese de Fármacos. Recife, PE, Brasil.Universidade Federal de Pernambuco (UFPE). Departamento de Antibióticos. Laboratório de Desenho e Síntese de Fármacos. Recife, PE, Brasil.Universidade Federal de Pernambuco (UFPE). Laboratório de Imunopatologia Keizo Asami. Recife, PE, Brasil / Universidade Federal de Pernambuco (UFPE). Departamento de Medicina Tropical. Recife, PE, Brasil.Universidade Federal de Pernambuco (UFPE). Departamento de Antibióticos. Laboratório de Desenho e Síntese de Fármacos. Recife, PE, Brasil.Schistosomiasis is a major public health problem with 207 million people infected and more than 779 million at risk. The drug of choice for treating schistosomiasis is praziquantel (PZQ); however, it is inefficient against immature forms of schistosomes. The aim of this study was to test new imidazolidine derivatives LPSF/PT09 and LPSF/PT10 against adult Schistosoma mansoni worms. IC50, cytotoxicity, immune response and cell viability assays were also available for these imidazolidines. Different concentrations of imidazolidine, from 32 to 320 ¼M, promoted motor abnormalities in breeding and unpaired worms, and death in 24 hours at higher concentrations. Although LPSF/PT09 and LPSF/PT10 did not affect IFN-³ and IL-10 production, they induced nitric oxide production and showed a similar behavior to praziquantel on cell death test. Thus, these new imidazolidine derivatives should undergo further study to develop schistosomiasis drugs

Synthesis of 4'-(2-ferrocenyl)-2,2':6'2''-terpyridine: characterization and antiprotozoal activity of Mn(II), Co(II), Ni(II), Cu(II) and Zn(II) complexes.

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2014-09-26T16:22:19Z No. of bitstreams: 1 Juneja A Synthesis of 4....pdf: 825777 bytes, checksum: 07c0ddff568600a860352d3f48610893 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2014-09-26T16:22:36Z (GMT) No. of bitstreams: 1 Juneja A Synthesis of 4....pdf: 825777 bytes, checksum: 07c0ddff568600a860352d3f48610893 (MD5)Made available in DSpace on 2014-09-26T16:29:40Z (GMT). No. of bitstreams: 1 Juneja A Synthesis of 4....pdf: 825777 bytes, checksum: 07c0ddff568600a860352d3f48610893 (MD5) Previous issue date: 2014Jamia Millia Islamia. Jamia Nagar. Department of Chemistry. New Delhi, IndiaFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilFederal University of Pernambuco. Centre for Health Sciences. Department of Pharmaceutical Sciences. Recife, PE, BrazilFundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Departamento de Imunologia. Recife, PE, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Departamento de Imunologia. Recife, PE, BrasilUniversidade da Coruña. Departamento de Química Fundamental. Coruña, SpainJamia Millia Islamia. Jamia Nagar. Department of Chemistry. New Delhi, IndiaA terpyridine ligand Fctpy was reacted with divalent metals (Cu, Co, Mn, Ni and Zn), yielding five complexes of general formula [Metal(Fctpy)2][PF6]2. The structure of Fctpy was determined by single crystal X-ray diffraction studies. The complexes characterized using various spectroscopic techniques suggested an octahedral geometry around the central metal ion. These complexes were screened for their antiamoebic, trypanocidal and antimalarial activities. It was found that, complexes 2 and 3 showed better IC50 values than metronidazole against HM1:IMSS strain of Entamoeba histolytica. A substantial parasitic inhibition was not observed for the trypanocidal activity. However, for the erythrocytic stage of W2 strain of Plasmodium falciparum, the complexes inhibited ß-hematin formation. At the concentration of 10 µg/mL, these complexes did not display toxicity