Phosphorylation Modulates the Subcellular Localization of SOX11

SOX11 is a key Transcription Factor (TF) in the regulation of embryonic and adult neurogenesis, whose mutation has recently been linked to an intellectual disability syndrome in humans. SOX11’s transient activity during neurogenesis is critical to ensure the precise execution of the neurogenic program. Here, we report that SOX11 displays differential subcellular localizations during the course of neurogenesis. Western-Blot analysis of embryonic mouse brain lysates indicated that SOX11 is post-translationally modified by phosphorylation. Using Mass Spectrometry, we found 10 serine residues in the SOX11 protein that are putatively phosphorylated. Systematic analysis of phospho-mutant SOX11 resulted in the identification of the S30 residue, whose phosphorylation promotes nuclear over cytoplasmic localization of SOX11. Collectively, these findings uncover phosphorylation as a novel layer of regulation of the intellectual disability gene Sox11

Gesundheitsförderung für und mit Jugendlichen und jungen Erwachsenen : wissenschaftliche Erkenntnisse und Empfehlungen für die Praxis

Die Zielgruppe der Jugendlichen und jungen Erwachsenen ist im Kontext der Corona-Pandemie in den Fokus gerückt. Wie wichtig es ist, die Gesundheit der Jugendlichen und jungen Erwachsenen zu fördern, wird durch diese Fokussierung betont. Vor diesem Hintergrund ist es wichtig, die wissenschaftlichen Grundlagen als Basis für wirksame Massnahmen und Interventionen zu aktualisieren. So ist sichergestellt, dass die Grundlage für Programme und Projekte auf dem neuesten Stand ist. Der vorliegende Bericht zeigt, weshalb sich ein Engagement für die Gesundheit von Jugendlichen und jungen Erwachsenen lohnt. Aufbauend auf wissenschaftlichen Erkenntnissen wird gezeigt, warum die Themen Bewegung, Ernährung und psychische Gesundheit wichtige Pfeiler für die Gesundheit im Jugend- und jungen Erwachsenenalter sind. Es werden Interventionen und bewährte Handlungsansätze und Empfehlungen vorgestellt. Autorinnen und Autoren: Kapitel 1 Einleitung und Kapitel 10 Schlussfolgerungen: Dr. phil. Fabienne Amstad Kapitel 2 Lebensphase: Prof. Dr. med. Joan-Carles Suris, Dr. Yara Barrense-Dias Kapitel 3 Grundlagen: Prof. Dr. med. Julia Dratva, Matthias Meyer, dipl. SozÖk, Prof. Dr. phil. Karin Nordström Kapitel 4 Chancengleichheit: lic. phil. Dominik Weber Kapitel 5 Medien: MSc Jael Bernath, Prof. Dr. Daniel Süss Kapitel 6 Bewegung: Prof. Dr. Suzanne Suggs Kapitel 7 Ernährung: Dr. Sophie Bucher Della Torre Kapitel 8 Psychische Gesundheit: Prof. Dr. Frank Wieber, Prof. Dr. Agnes von Wyl, Dr. Annina Zysset Kapitel 9 Zusammenspiel: MSc Ronia Schiftan, MSc Anne-Françoise Wittgenstein Man

Image_2_Phosphorylation Modulates the Subcellular Localization of SOX11.TIF

<p>SOX11 is a key Transcription Factor (TF) in the regulation of embryonic and adult neurogenesis, whose mutation has recently been linked to an intellectual disability syndrome in humans. SOX11’s transient activity during neurogenesis is critical to ensure the precise execution of the neurogenic program. Here, we report that SOX11 displays differential subcellular localizations during the course of neurogenesis. Western-Blot analysis of embryonic mouse brain lysates indicated that SOX11 is post-translationally modified by phosphorylation. Using Mass Spectrometry, we found 10 serine residues in the SOX11 protein that are putatively phosphorylated. Systematic analysis of phospho-mutant SOX11 resulted in the identification of the S30 residue, whose phosphorylation promotes nuclear over cytoplasmic localization of SOX11. Collectively, these findings uncover phosphorylation as a novel layer of regulation of the intellectual disability gene Sox11.</p

Table_1_Phosphorylation Modulates the Subcellular Localization of SOX11.XLSX

<p>SOX11 is a key Transcription Factor (TF) in the regulation of embryonic and adult neurogenesis, whose mutation has recently been linked to an intellectual disability syndrome in humans. SOX11’s transient activity during neurogenesis is critical to ensure the precise execution of the neurogenic program. Here, we report that SOX11 displays differential subcellular localizations during the course of neurogenesis. Western-Blot analysis of embryonic mouse brain lysates indicated that SOX11 is post-translationally modified by phosphorylation. Using Mass Spectrometry, we found 10 serine residues in the SOX11 protein that are putatively phosphorylated. Systematic analysis of phospho-mutant SOX11 resulted in the identification of the S30 residue, whose phosphorylation promotes nuclear over cytoplasmic localization of SOX11. Collectively, these findings uncover phosphorylation as a novel layer of regulation of the intellectual disability gene Sox11.</p

Table_2_Phosphorylation Modulates the Subcellular Localization of SOX11.XLSX

<p>SOX11 is a key Transcription Factor (TF) in the regulation of embryonic and adult neurogenesis, whose mutation has recently been linked to an intellectual disability syndrome in humans. SOX11’s transient activity during neurogenesis is critical to ensure the precise execution of the neurogenic program. Here, we report that SOX11 displays differential subcellular localizations during the course of neurogenesis. Western-Blot analysis of embryonic mouse brain lysates indicated that SOX11 is post-translationally modified by phosphorylation. Using Mass Spectrometry, we found 10 serine residues in the SOX11 protein that are putatively phosphorylated. Systematic analysis of phospho-mutant SOX11 resulted in the identification of the S30 residue, whose phosphorylation promotes nuclear over cytoplasmic localization of SOX11. Collectively, these findings uncover phosphorylation as a novel layer of regulation of the intellectual disability gene Sox11.</p

Image_4_Phosphorylation Modulates the Subcellular Localization of SOX11.TIF

<p>SOX11 is a key Transcription Factor (TF) in the regulation of embryonic and adult neurogenesis, whose mutation has recently been linked to an intellectual disability syndrome in humans. SOX11’s transient activity during neurogenesis is critical to ensure the precise execution of the neurogenic program. Here, we report that SOX11 displays differential subcellular localizations during the course of neurogenesis. Western-Blot analysis of embryonic mouse brain lysates indicated that SOX11 is post-translationally modified by phosphorylation. Using Mass Spectrometry, we found 10 serine residues in the SOX11 protein that are putatively phosphorylated. Systematic analysis of phospho-mutant SOX11 resulted in the identification of the S30 residue, whose phosphorylation promotes nuclear over cytoplasmic localization of SOX11. Collectively, these findings uncover phosphorylation as a novel layer of regulation of the intellectual disability gene Sox11.</p