The nitrogen problem of epiphytic lichens – a synthesis

Der starke Anstieg von nitrophytischen Flechten während der letzten 15 Jahren in landwirtschaftlich genutzten und städtischen Bereichen hat die Frage aufgeworfen, welche Stickstoffverbindung dafür verantwortlich sind, welche physiologischen Probleme diese machen, wo diese herkommen, welche Auswirkungen sie auf die Flechten haben, was mit dem Stickstoff in den Flechten passiert, wie sich Feinstaub und trockene Deposition auswirken und wieso Nitrophyten auch in unbelasteten Gebieten dominieren. Nach den Ergebnissen zahlreicher Einzelstudien, die hier mit neuen Daten ergänzt werden, ist Ammoniak die relevante Stickstoffquelle. Dieser wird als Ammoniumnitrat speziell in Form von trockener Deposition von den Flechten aufgenommen. Da Ammoniumnitrat ein Salz ist, haben Nitrophyten höhere osmotische Werte, weswegen sie in belasteten Gebieten konkurrenzkräftiger sind. Dies erlaubt Nitrophyten gleichzeitig die Existenz in Trockengebieten auch ohne Stickstoffemissionen, weswegen sie nicht unbedingt Stickstoffzeiger sondern auch Trockenzeiger sind. Der anfallende Stickstoff wird in Form von Aminosäuren in der Flechte passiv gespeichert, weswegen Nitrophyten nicht nitrophil sondern nitrotolerant, halotolerant und xerophytisch sind. Die momentane Temperaturerhöhung und die damit verbundene erhöhte Verdunstung sowie die Zunahme trockener Deposition (Feinstaubbelastung) führen zur Zeit trotz sinkender Gesamtstickstoffbelastung zu einem weiteren Anstieg der nitrophytischen Flechten.The strong increase of nitrophytic lichens in Central Europe during the past fifteen years in urban regions and regions with intensive agriculture poses the question, which nitrogen compounds are responsible, where they come from, which effects they have on lichens, what happens with the nitrogen in lichens, how particulate matter and dry deposition influence lichens und why nitrophytic lichens dominate also in regions with no or low nitrogen emissions. According to several studies, which are complete by new data, ammonia is the relevant source of nitrogen. It is taken up by lichens as ammoniumnitrate as dry deposition. Ammoniumnitrate, however, is a salt. Accordingly nitrophytic lichens have higher osmotic values. This enables nitrophytic lichens to survive in drier regions as compared with other epiphytic lichens, where they are more competitive, even without nitrogen emissions. Therefore nitrophytic lichens are not nitrophilous but halotolerant and xerophytic. The present increase of temperatures, which raises the evaporation, and the increase of dry deposition (particulate matter) cause an increase of nitrophytic lichens in spite of decrease total nitrogen emissions

The D coefficient in neutron beta decay in effective field theory

In this paper we explore the time-reversal-odd triple-correlation coefficient in neutron beta decay, the so-called "D coefficient", using heavy-baryon effective field theory with photon degrees of freedom. We find that this framework allows us to reproduce the known results for the contribution which comes from final-state interactions, and also to discuss higher-order corrections. In particular we are able to show that in the heavy-baryon limit all electromagnetic contributions vanish. By calculating the leading correction to the known result, we give a final expression which is accurate to better than 1%. Hence we extend downwards the range over which the D coefficient could be used to explore time-violation from new physics.Comment: 12c pages, 3 eps figures Version accepted for publication in Physics Letters B; minor changes of wordin

Reconstructing Native American Population History

The peopling of the Americas has been the subject of extensive genetic, archaeological and linguistic research; however, central questions remain unresolved1–5. One contentious issue is whether the settlement occurred via a single6–8 or multiple streams of migration from Siberia9–15. The pattern of dispersals within the Americas is also poorly understood. To address these questions at higher resolution than was previously possible, we assembled data from 52 Native American and 17 Siberian groups genotyped at 364,470 single nucleotide polymorphisms. We show that Native Americans descend from at least three streams of Asian gene flow. Most descend entirely from a single ancestral population that we call “First American”. However, speakers of Eskimo-Aleut languages from the Arctic inherit almost half their ancestry from a second stream of Asian gene flow, and the Na-Dene-speaking Chipewyan from Canada inherit roughly one-tenth of their ancestry from a third stream. We show that the initial peopling followed a southward expansion facilitated by the coast, with sequential population splits and little gene flow after divergence, especially in South America. A major exception is in Chibchan-speakers on both sides of the Panama Isthmus, who have ancestry from both North and South America

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Risk evaluation of duodenoscope-associated infections in the Netherlands calls for a heightened awareness of device-related infections: A systematic review

Background The risk of exogenous infections from endoscopic procedures is often cited as almost negligible (1 infection in 1.8 million procedures); however, this risk is based on older literature and does not seem to match the number of infectious outbreaks due to contaminated duodenoscopes reported after endoscopic retrograde cholangiopancreatography (ERCP). Using Dutch data, we aimed to estimate the minimum risk of duodenoscope-associated infection (DAI) and colonization (DAC) in patients undergoing ERCP. Methods A systematic literature search identified all DAI outbreaks in the Netherlands reported between 2008 and 2019. Included cases were confirmed by molecular matching of patient and duodenoscope cultures. Risk ratios were calculated based on the total number of ERCPs performed during the study period. Results Three outbreaks were reported and published between 2008 and 2018, including 21 confirmed DAI cases and 52 confirmed DAC cases. The estimated number of ERCPs performed during the same period was 181 209-227 006. The calculated minimum estimated DAI risk was approximately 0.01 % and the minimum estimated DAC risk was 0.023 %-0.029 %. Conclusions The estimated risk of DAI in Dutch ERCP practice was at least 180 times higher than previously published risk estimates. The actual risk is likely to be (much) higher due to underreporting of infections caused by multidrug-resistant organisms and sensitive bacteria. Greater awareness by healthcare personnel involved in endoscopy and endoscope cleaning is required, as well as innovative technical solutions to contain and ultimately eliminate DAIs

Main Sources, Socio-Demographic and Anthropometric Correlates of Salt Intake in Austria

Excessive salt intake is known to increase blood pressure and cardiovascular risk. Nevertheless, salt intake exceeds the recommendations in most countries. To face this problem, it is important to identify high consumers as well as the main contributors of salt intake. Overall, data of 2018 adults between 18 and 64 years were analysed to determine the main sources, socio-demographic and anthropometric correlates of salt intake. Dietary intake was assessed from 24-h-recalls, information on socio-demographic characteristics was obtained using a questionnaire and anthropometric data were measured. Salt intake was significantly higher in males than in females. There was a significant positive association between salt intake and body mass index. No significant differences in salt intake were observed for other variables including affluence, educational level, smoking status and physical activity. The main contributor to salt intake were condiments including table salt (32.6%), followed by cereals and cereal products (27.0%), meat and meat products (16.1%) and dairy products (14.0%). These results highlight that specific population groups need to be targeted by public health initiatives and that a reduction in salt intake can only be achieved in tandem with the food producers by the reduction of salt in processed foods

Methane emissions partially offset blue carbon burial in mangroves

Organic matter burial in mangrove forests results in the removal and long-term storage of atmospheric CO2, so-called blue carbon. However, some of this organic matter is metabolized and returned to the atmosphere as CH4. Because CH4 has a higher global warming potential than the CO2 fixed in the organic matter, it can offset the CO2 removed via carbon burial. We provide the first estimate of the global magnitude of this offset. Our results show that high CH4 evasion rates have the potential to partially offset blue carbon burial rates in mangrove sediments on average by 20% (sensitivity analysis offset range, 18 to 22%) using the 20-year global warming potential. Hence, mangrove sediment and water CH4 emissions should be accounted for in future blue carbon assessments

Estuaries as sources and sinks of N2O across a land‐use gradient in subtropical Australia

Intensifying agricultural production and coastal urbanization are increasing nitrogen (N) loads to estuaries, potentially increasing emissions of the greenhouse gas nitrous oxide (N₂O). Here we present a first assessment of how changes in land use intensity affect estuarine N₂O fluxes. We measured N₂O concentrations over marine-freshwater transects in the wet and dry seasons in eight subtropical estuaries selected for differences in land use intensity. Daily estuary N loads ranged from 0.5 ± 0.4 kg N km¯² d¯¹ (minimally impacted) to 51 ± 30 kg N km¯² d¯¹ (highly impacted), corresponding to higher concentrations of all inorganic N species (nitrate, ammonium, and N₂O) in the highly impacted estuaries. Net N₂O fluxes from the eight estuaries ranged from −20 μg N₂O-N km¯² d¯¹ (sink) to +300 μg N₂O-N km¯² d¯¹(source). However, neither N concentrations nor N loads explained the variations in N₂O fluxes. Instead, seasonal differences in freshwater flushing times increased either N₂O uptake (minimally impacted systems) or N₂O efflux (moderately impacted systems) relative to N load. The lack of relationship between freshwater flushing times (kinetics) and N₂O fluxes from the highly impacted estuaries, combined with evidence for both low carbon quality and phosphorous limitation in those systems, suggests that N₂O emissions from highly impacted estuaries were controlled by stoichiometry rather than kinetics. This study shows that estuaries can shift from net sinks to sources of N₂O as land use intensity increases but that the magnitude of this switch cannot be predicted based on N loads alone