Seudomicetoma dermatofítico en un gato persa : aspectos clínicos, patológicos y evolutivos

En este trabajo presentamos los aspectos clínicos, anatomopatológicos y evolutivos de un caso de dermatitis piogranulomatosa múltiple causada por hongos del género Microsporum, también conocida como seudomicetoma dermatofítico, en un gato persa, no castrado, de un año de edad al comienzo de la enfermedad. Las lesiones se caracterizaron macroscópicamente por la formación de numerosos nódulos de diferentes tamaños, localizados en la cabeza y antebrazo derecho, extendiéndose posteriormente a zona dorso lateral del tronco. La evolución de estos nódulos, que se generalizaron en algo menos de un mes, era hacia la coalescencia y fistulización, con descarga de un material grumoso blanco-grisáceo. El estudio histopatológico reveló una dermatitis piogranulomatosa, supeficial y profunda, caracterizada por presentar en el centro de los granulomas agregados de hongos septados inmersos en una matriz acidófila. La extirpación quirúrgica de un gran número de nódulos proporcionó mejorías transitorias, aunque reaparecieron nuevas lesiones. En este trabajo se describe la evolución tras dos años de seguimiento clínico. Tras el tratamiento con griseofulvina no se detectan nuevas lesiones después de 6 meses de su aplicación y seguimiento.This paper reports the clinical and histopathological features of a case of pyogranulomatous dermatitis caused by Microsporum fungi, a condition also known as dermatophyte pseudomycetoma, in a eneyear-old Persian cat. Grossly apparent fistulized nodules of varying sizes displaying a granular discharge were localized on the head, back, elbow and tail. Microscopic analysis revealed a deep piogranulomatous dermatitis characterized by centrally-located clusters of fungi within an acidophilic matrix. In just under a month, nodules had beco me generalized. Partial surgical excision and non-specific treatment provided temporary improvement, but new lesions subsequentlyappeared

Furrow and Ridge Soil Nitrogen Mineralization in a Surface Irrigated Artichoke Field

[EN] Quantitative knowledge of soil organic nitrogen net mineralization (NNM) in field conditions is crucial to optimize N fertilization of crops. In a field fertilization trial of artichokes 48 PE tubes were inserted to 20 cm depth in the soil in plant row and irrigation furrows and soil samples were periodically taken during two and a half months to determine NNM. A parallel essay with disturbed samples from the same procedence was carried out in the laboratory at 25ºC and 10 kPa soil water tension. Soil sample position (ridge and furrow) did not significantly determined NNM in the laboratory essay. Although NNM (obtained from laboratory incubation and corrected to field soil temperature and moisture monitored during the experimental period) overpredicted measured field NNM, matching of both was better than those reported in other studies. NNM rate for the 76 days period of incubation predicted from lab data was 22.9 kg N/ ha x 0.1 m while corresponding field values corrected by Br- or Cl- mass balance were 10% and 20% lower respectively in ridge position and under 40% lower by either method in furrow position.The research reported in this paper was supported by a fund from CICYT-INIA (project RTA01-117-C2-2)Lidón, A.; Bautista, I.; De La Iglesia, F.; Oliver Talens, J.; Llorca, R.; Cruz-Romero, G. (2006). Furrow and Ridge Soil Nitrogen Mineralization in a Surface Irrigated Artichoke Field. Acta Horticulturae. (700):71-74. https://doi.org/10.17660/ActaHortic.2006.700.7S717470

Changes in neurosteroidogenesis during demyelination and remyelination in cuprizone-treated mice

Changes of neurosteroids may be involved in the pathophysiology of multiple sclerosis (MS). The present study investigated whether changes of neurosteroidogenesis also occurred in the grey and white matter regions of the brain in mice subjected to cuprizone‐induced demyelination. Accordingly, we compared the expression of neurosteroidogenic proteins, including steroidogenic acute regulatory protein (StAR), voltage‐dependent anion channel (VDAC) and 18 kDa translocator protein (TSPO), as well as neurosteroidogenic enzymes, including the side chain cleavage enzyme (P450scc), 3β‐hydroxysteroid dehydrogenase/isomerase and 5α‐reductase (5α‐R), during the demyelination and remyelination periods. Using immunohistochemistry and a quantitative polymerase chain reaction, we demonstrated a decreased expression of StAR, P450scc and 5α‐R with respect to an increase astrocytic and microglial reaction and elevated levels of tumor necrosis factor (TNF)α during the cuprizone demyelination period in the hippocampus, cortex and corpus callosum. These parameters, as well as the glial reaction, were normalised after 2 weeks of spontaneous remyelination in regions containing grey matter. Conversely, persistent elevated levels of TNFα and low levels of StAR and P450scc were observed during remyelination in corpus callosum white matter. We conclude that neurosteroidogenesis/myelination status and glial reactivity are inversely related in the hippocampus and neocortex. Establishing a cause and effect relationship for the measured variables remains a future challenge for understanding the pathophysiology of MS.Fil: Leicaj, María L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Pasquini, Laura Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Lima, Analia Ethel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Gonzalez Deniselle, Maria Claudia. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Pasquini, Juana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: De Nicola, Alejandro F.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Garay, Laura Ines. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentin

Efficacy of treatment for hyperglycemic crisis in elderly diabetic patients in a day hospital

The purpose of this prospective cohort study was to compare the costs of day hospital (DH) care for hyperglycemic crisis in elderly diabetic patients with those of conventional hospitalization (CH). Secondary objectives were to compare these two clinical scenarios in terms of glycemic control, number of emergency and outpatient visits, readmissions, hypoglycemic episodes, and nosocomial morbidity. The study population comprised diabetic patients aged >74 years consecutively admitted to a tertiary teaching hospital in Spain for hyperglycemic crisis (sustained hyperglycemia [>300 mg/dL] for at least 3 days with or without ketosis). The patients were assigned to DH or CH care according to time of admission and were followed for 6 months after discharge. Exclusion criteria were ketoacidosis, hyperosmolar crisis, hemodynamic instability, severe intercurrent illness, social deprivation, or Katz index >D. Sixty-four diabetic patients on DH care and 36 on CH care were included, with no differences in baseline characteristics. The average cost per patient was 1,345.1±793.6 € in the DH group and 2,212.4±982.5 € in the CH group (P <0.001). There were no differences in number of subjects with mild hypoglycemia during follow-up (45.3% DH versus 33.3% CH, P =0.24), nor in the percentage of patients achieving a glycated hemoglobin (HbA) <8% (67.2% DH versus 58.3% CH, P =0.375). Readmissions for hyperglycemic crisis and pressure ulcer rates were significantly higher in the CH group. DH care for hyperglycemic crises is more cost-effective than CH care, with a net saving of 1,418.4 € per case, lower number of readmissions and pressure ulcer rates, and similar short-term glycemic control and hypoglycemia rates

Apotransferrin-induced recovery after hypoxic/ischaemic injury on myelination

We have previously demonstrated that aTf (apotransferrin) accelerates maturation of OLs (oligodendrocytes) in vitro as well as in vivo. The purpose of this study is to determine whether aTf plays a functional role in a model of H/I (hypoxia/ischaemia) in the neonatal brain. Twenty-four hours after H/I insult, neonatal rats were intracranially injected with aTf and the effects of this treatment were evaluated in the CC (corpus callosum) as well as the SVZ (subventricular zone) at different time points. Similar to previous studies, the H/I event produced severe demyelination in the CC. Demyelination was accompanied by microglial activation, astrogliosis and iron deposition. Ferritin levels increased together with lipid peroxidation and apoptotic cell death. Histological examination after the H/I event in brain tissue of aTf-treated animals (H/I aTF) revealed a great number of mature OLs repopulating the CC compared with saline-treated animals (H/I S). ApoTf treatment induced a gradual increase in MBP (myelin basic protein) and myelin lipid staining in the CC reaching normal levels after 15 days. Furthermore, significant increase in the number of OPCs (oligodendroglial progenitor cells) was found in the SVZ of aTf-treated brains compared with H/I S. Specifically, there was a rise in cells positive for OPC markers, i.e. PDGFRα and SHH+ cells, with a decrease in cleaved-caspase-3+ cells compared with H/I S. Additionally, neurospheres from aTf-treated rats were bigger in size and produced more O4/MBP+ cells. Our findings indicate a role for aTf as a potential inducer of OLs in neonatal rat brain in acute demyelination caused by H/I and a contribution to the differentiation/maturation of OLs and survival/migration of SVZ progenitors after demyelination in vivo

Seven-year mortality in heart failure patients with undiagnosed diabetes : an observational study

Background: Patients with type 2 diabetes mellitus and heart failure have adverse clinical outcomes, but the characteristics and prognosis of those with undiagnosed diabetes in this setting has not been established. Methods: In total, 400 patients admitted consecutively with acute heart failure were grouped in three glycaemic categories: no diabetes, clinical diabetes (previously reported or with hypoglycaemic treatment) and undiagnosed diabetes. The latter was defined by the presence of at least two measurements of fasting plasma glycaemia ≥ 7 mmol/L before or after the acute episode.Group differences were tested by proportional hazards models in all-cause and cardiovascular mortality during a 7-year follow-up. Results: There were 188 (47%) patients without diabetes, 149 (37%) with clinical diabetes and 63 (16%) with undiagnosed diabetes. Patients with undiagnosed diabetes had a lower prevalence of hypertension, dyslipidaemia, peripheral vascular disease and previous myocardial infarction than those with clinical diabetes and similar to that of those without diabetes. The adjusted hazards ratios for 7-year total and cardiovascular mortality compared with the group of subjects without diabetes were 1.69 (95% CI: 1.17-2.46) and 2.45 (95% CI: 1.58-3.81) for those with undiagnosed diabetes, and 1.48 (95% CI: 1.10-1.99) and 2.01 (95% CI: 1.40-2.89) for those with clinical diabetes. Conclusions: Undiagnosed diabetes is common in patients requiring hospitalization for acute heart failure. Patients with undiagnosed diabetes, despite having a lower cardiovascular risk profile than those with clinical diabetes, show a similar increased mortality

Synthesis Of Ag@silica Nanoparticles By Assisted Laser Ablation.

This paper reports the synthesis of silver nanoparticles coated with porous silica (Ag@Silica NPs) using an assisted laser ablation method. This method is a chemical synthesis where one of the reagents (the reducer agent) is introduced in nanometer form by laser ablation of a solid target submerged in an aqueous solution. In a first step, a silicon wafer immersed in water solution was laser ablated for several minutes. Subsequently, an AgNO3 aliquot was added to the aqueous solution. The redox reaction between the silver ions and ablation products leads to a colloidal suspension of core-shell Ag@Silica NPs. The influence of the laser pulse energy, laser wavelength, ablation time, and Ag(+) concentration on the size and optical properties of the Ag@Silica NPs was investigated. Furthermore, the colloidal suspensions were studied by UV-VIS-NIR spectroscopy, X-Ray diffraction, and high-resolution transmission electron microscopy (HRTEM).1039

Localización de zonas de cizalla y de contactos intrusivos en la Sierra de Velasco (Argentina), mediante análisis gravimétrico

Mediante la aplicación de la metodología de Deconvolución de Euler, se pueden identificar y hacer estimaciones de profundidad para una variedad de estructuras geológicas tales como fallas, contactos, diques, plutones, etc. La gran ventaja de esta técnica es que no asume ningún modelo geológico en particular, pudiendo aplicarse directamente a datos regularizados. La aplicación de este método a la carta gravimétrica de Sierra de Velasco (con índices estructurales que variaron entre 0,5, 1Y2) permitió obtener un conjunto de soluciones, en un rango de profundidades que van desde unos pocos metros hasta 24 km. Estas soluciones pueden aplicarse a discontinuidades litológicas de la corteza, tales como la zona de cizalla TIPA,o bien el plano de intrusivo entre el plutónSan Bias con el Granito Asha. Estas discontinuidades geológicas son observadas en superficie, pero la determinación y continuación en profundidad no era posiblecon herramientasgeológicas tradicionales. Se identifican además, dos sistemas de fallas inversas que elevan la sierra (por el noreste y por el noroeste), que son de tipo inverso y se hacen lístricas en profundidad. Las mismas como se sabe responden a la orogenia Andina, que levantan las Sierras Pampeanas, dándole la actual configuración morfológicaa laSierra deVelasco.By means of the Eu/er Deeonvolueión methodology, they ean be identified and depth estima te for a variety of ge%gie struetures, sueh as fau/ts, eontacts, dikes, p/utons, etc. The great advantage of this technique is that it does not assume any particular ge%gic pattem, being been ab/e to app/y directly to sprouted data. The app/ieation of this method to the gravimetric chart of the Sierra de Ve/asco (with struetura/ indexes that varied between 0.5, 1 and 2) it allowed to obtain a group of so/utions, in a range of depths that they go from some few meters to 24 km. These so/utions can be applied to the /ith%gic diseontinuities of the erust, such as the shear TlPA area, or the intrusive eontact p/ane between the San B/as p/uton and the Asha granite. These ge%gic discontinuities are observed in surface, but the determination and continuation in depth it was not possib/e. Theyare a/so idenfified, two systems of in verse faults that e/evate the mountains (for the northeast and for the northwest). These faults are of upthrust type and they are made listrie in depth. These fau/ts as ii is known they respond to the Andean orogeny that the Sierras Pampeanas get up, giving the current motpñoloqic conñquretion to the Sierra of Ve/asco.Fil: Martinez, M. Patricia. Universidad Nacional de San Juan. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto Geofísico Sismológico Volponi; ArgentinaFil: Gimenez, Mario Ernesto. Universidad Nacional de San Juan. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto Geofísico Sismológico Volponi; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Toselli, Alejandro Jose. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Departamento de Geología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rossi de Toselli, Juana. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Departamento de Geología; ArgentinaFil: Ruiz, F.. Universidad Nacional de San Juan. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto Geofísico Sismológico Volponi; ArgentinaFil: Introcaso, Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; ArgentinaFil: Novara, Ivan Lionel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; Argentin