A composite 10Be, IR-50 and 14C chronology of the pre-LGM full ice extent of the western Patagonian Ice Sheet in the Isla de Chiloé, south Chile (42ºS

Unanswered questions about the glacier and climate history preceding the global Last Glacial Maximum (LGM) in the southern temperate latitudes remain. The Marine Isotope Stage (MIS) 3 is normally understood as a global interstadial period; nonetheless its climate was punctuated by conspicuous variability, and its signature has not been resolved beyond the polar realms. In this paper, we compile a 10Be depth profile, single grain infrared (IR) stimulated luminescence dating and 14C samples to derive a new glacier record for the principal outwash plain complex, deposited by the western Patagonian Ice Sheet (PIS) during the last glacial period (Llanquihue Glaciation) on the Isla de Chiloé, southern Chile (42∘ S). In this region, the Golfo de Corcovado Ice Lobe left a distinct geomorphic and stratigraphic imprint, suitable for reconstructing former ice dynamics and timing of past climate change. Our data indicate that maximum glaciation occurred by 57.8±4.7 ka without reaching the Pacific Ocean coast. Ice readavanced and buttressed against the eastern side of the Cordillera de la Costa again by 26.0±2.9 ka. Our data further support the notion of a large ice extent during parts of the MIS 3 in Patagonia and New Zealand but appear to contradict near contemporaneous interstadial evidence in the southern midlatitudes, including Chiloé. We propose that the PIS expanded to its full-glacial Llanquihue moraines, recording a rapid response of southern mountain glaciers to the millennial-scale climate stadials that punctuated the MIS 3 at the poles and elsewhere.</p

Enhancing online knowledge graph population with semantic knowledge

Knowledge Graphs (KG) are becoming essential to organize, represent and store the world’s knowledge, but they still rely heavily on humanly-curated structured data. Information Extraction (IE) tasks, like disambiguating entities and relations from unstructured text, are key to automate KG population. However, Natural Language Processing (NLP) methods alone can not guarantee the validity of the facts extracted and may introduce erroneous information into the KG. This work presents an end-to-end system that combines Semantic Knowledge and Validation techniques with NLP methods, to provide KG population of novel facts from clustered news events. The contributions of this paper are two-fold: First, we present a novel method for including entity-type knowledge into a Relation Extraction model, improving F1-Score over the baseline with TACRED and TypeRE datasets. Second, we increase the precision by adding data validation on top of the Relation Extraction method. These two contributions are combined in an industrial pipeline for automatic KG population over aggregated news, demonstrating increased data validity when performing online learning from unstructured web data. Finally, the TypeRE and AggregatedNewsRE datasets build to benchmark these results are also published to foster future research in this field.This work was partially supported by the Government of Catalonia under the industrial doctorate 2017 DI 011.Peer ReviewedPostprint (author's final draft

Hepatitis G virus infection in chronic liver disease

Background¿The hepatitis G virus (HGV), a recently identified member of the Flaviviridae family, can cause chronic infection in man but the role of this agent in chronic liver disease is poorly understood. Aims¿To evaluate the prevalence and meaning of HGV infection in a large series of patients with chronic liver disease. Subjects¿Two hundred volunteer blood donors, 179 patients with chronic hepatitis C, 111 with chronic hepatitis B, 104 with alcoholic liver disease, 136 with hepatocellular carcinoma, and 24 with cryptogenic chronic liver disease were studied. Methods¿HGV RNA was investigated in serum samples by reverse transcription and polymerase chain reaction amplification of the 5¿ non-coding region of HCV and hybridisation to a specific probe. The main features of HGV RNA seropositive and seronegative patients were compared. Results¿The prevalence of HGV infection was 3% in blood donors, 7% in chronic hepatitis C, 8% in chronic hepatitis B, 2% in alcoholic liver disease, 4% in hepatocellular carcinoma, and 8% in cryptogenic chronic liver disease. HGV infected patients tended to be younger than non-infected patients but no differences concerning sex, possible source of infection, clinical manifestations, biochemical and virological parameters, or severity of liver lesions were found. Conclusions¿The prevalence of HGV infection in chronic liver disease seems to be relatively low in our area. Infection with HGV does not seem to play a significant pathogenic role in patients with chronic liver disease related to chronic HBV or HCV infection or to increased alcohol consumption, or in those with cryptogenic chronic liver disease

Effect of resveratrol on alcohol-induced mortality and liver lesions in mice

BACKGROUND: Resveratrol is a polyphenol with important antiinflammatory and antioxidant properties. We investigated the effect of resveratrol on alcohol-induced mortality and liver lesions in mice. METHODS: Mice were randomly distributed into four groups (control, resveratrol-treated control, alcohol and resveratrol-treated alcohol). Chronic alcohol intoxication was induced by progressively administering alcohol in drinking water up to 40% v/v. The mice administered resveratrol received 10 mg/ml in drinking water. The animals had free access to standard diet. Blood levels were determined for transaminases, IL-1 and TNF-α. A histological evaluation was made of liver damage, and survival among the animals was recorded. RESULTS: Transaminase concentration was significantly higher in the alcohol group than in the rest of the groups (p < 0.05). IL-1 levels were significantly reduced in the alcohol plus resveratrol group compared with the alcohol group (p < 0.05). TNF-α was not detected in any group. Histologically, the liver lesions were more severe in the alcohol group, though no significant differences between groups were observed. Mortality in the alcohol group was 78% in the seventh week, versus 22% in the alcohol plus resveratrol group (p < 0.001). All mice in the alcohol group died before the ninth week. CONCLUSION: The results obtained suggest that resveratrol reduces mortality and liver damage in mice

Effectiveness of PRECEDE model for health education on changes and level of control of HbA1c, blood pressure, lipids, and body mass index in patients with type 2 diabetes mellitus

<p>Abstract</p> <p>Background</p> <p>Individual health education is considered to be essential in the overall care of patients with type 2 diabetes (DM2), although there is some uncertainty regarding its metabolic control benefits. There have been very few randomized studies on the effects of individual education on normal care in DM2 patients with a control group, and none of these have assessed the long-term results. Therefore, this study aims to use this design to assess the effectiveness of the PRECEDE (Predisposing, Reinforcing, Enabling, Causes in Educational Diagnosis, and Evaluation) education model in the metabolic control and the reduction of cardiovascular risk factors, in patients with type 2 diabetes.</p> <p>Methods</p> <p>An open community effectiveness study was carried out in 8 urban community health centers in the North-East Madrid Urban Area (Spain). Six hundred patients with DM2 were randomized in two groups: PRECEDE or conventional model for health promotion education. The main outcome measures were glycated hemoglobin A1c, body mass index (BMI), blood pressure, lipids and control criteria during the 2-year follow-up period.</p> <p>Results</p> <p>Glycated hemoglobin A1c and systolic blood pressure (SBP) levels decreased significantly in the PRECEDE group (multivariate analysis of covariance, with baseline glycated hemoglobin A1c, SBP, and variables showing statistically significant differences between groups at baseline visits). The decrease levels in diastolic blood pressure (DBP), triglycerides and LDL cholesterol were nonsignificant. PRECEDE increased compliance in all control criteria, except for LDL cholesterol. BMI did not change during the study in either of the two models analyzed.</p> <p>Conclusions</p> <p>PRECEDE health education model is a useful method in the overall treatment in patients with type 2 diabetes, which contributes to decrease glycated hemoglobin A1c and SBP levels and increase the compliance in all the control criteria, except for LDL cholesterol.</p> <p>Trial registration number</p> <p>ClinicalTrials.gov <a href="http://www.clinicaltrials.gov/ct2/show/NCT01316367">NCT01316367</a></p

Management and outcomes of patients with left atrial appendage thrombus prior to percutaneous closure.

Left atrial appendage (LAA) thrombus has heretofore been considered a contraindication to percutaneous LAA closure (LAAC). Data regarding its management are very limited. The aim of this study was to analyse the medical and invasive treatment of patients referred for LAAC in the presence of LAA thrombus. This multicentre observational registry included 126 consecutive patients referred for LAAC with LAA thrombus on preprocedural imaging. Treatment strategies included intensification of antithrombotic therapy (IAT) or direct LAAC. The primary and secondary endpoints were a composite of bleeding, stroke and death at 18 months, and procedural success, respectively. IAT was the preferred strategy in 57.9% of patients, with total thrombus resolution observed in 60.3% and 75.3% after initial and subsequent IAT, respectively. Bleeding complications and stroke during IAT occurred in 9.6% and 2.9%, respectively, compared with 3.8% bleeding and no embolic events in the direct LAAC group before the procedure. Procedural success was 90.5% (96.2% vs 86.3% in direct LAAC and IAT group, respectively, p=0.072), without cases of in-hospital thromboembolic complications. The primary endpoint occurred in 29.3% and device-related thrombosis was found in 12.8%, without significant difference according to treatment strategy. Bleeding complications at 18 months occurred in 22.5% vs 10.5% in the IAT and direct LAAC group, respectively (p=0.102). In the presence of LAA thrombus, IAT was the initial management strategy in half of our cohort, with initial thrombus resolution in 60% of these, but with a relatively high bleeding rate (~10%). Direct LAAC was feasible, with high procedural success and absence of periprocedural embolic complications. However, a high rate of device-related thrombosis was detected during follow-up

Spatiotemporal Characteristics of the Largest HIV-1 CRF02_AG Outbreak in Spain: Evidence for Onward Transmissions

Background and Aim: The circulating recombinant form 02_AG (CRF02_AG) is the predominant clade among the human immunodeficiency virus type-1 (HIV-1) non-Bs with a prevalence of 5.97% (95% Confidence Interval-CI: 5.41–6.57%) across Spain. Our aim was to estimate the levels of regional clustering for CRF02_AG and the spatiotemporal characteristics of the largest CRF02_AG subepidemic in Spain.Methods: We studied 396 CRF02_AG sequences obtained from HIV-1 diagnosed patients during 2000–2014 from 10 autonomous communities of Spain. Phylogenetic analysis was performed on the 391 CRF02_AG sequences along with all globally sampled CRF02_AG sequences (N = 3,302) as references. Phylodynamic and phylogeographic analysis was performed to the largest CRF02_AG monophyletic cluster by a Bayesian method in BEAST v1.8.0 and by reconstructing ancestral states using the criterion of parsimony in Mesquite v3.4, respectively.Results: The HIV-1 CRF02_AG prevalence differed across Spanish autonomous communities we sampled from (p &lt; 0.001). Phylogenetic analysis revealed that 52.7% of the CRF02_AG sequences formed 56 monophyletic clusters, with a range of 2–79 sequences. The CRF02_AG regional dispersal differed across Spain (p = 0.003), as suggested by monophyletic clustering. For the largest monophyletic cluster (subepidemic) (N = 79), 49.4% of the clustered sequences originated from Madrid, while most sequences (51.9%) had been obtained from men having sex with men (MSM). Molecular clock analysis suggested that the origin (tMRCA) of the CRF02_AG subepidemic was in 2002 (median estimate; 95% Highest Posterior Density-HPD interval: 1999–2004). Additionally, we found significant clustering within the CRF02_AG subepidemic according to the ethnic origin.Conclusion: CRF02_AG has been introduced as a result of multiple introductions in Spain, following regional dispersal in several cases. We showed that CRF02_AG transmissions were mostly due to regional dispersal in Spain. The hot-spot for the largest CRF02_AG regional subepidemic in Spain was in Madrid associated with MSM transmission risk group. The existence of subepidemics suggest that several spillovers occurred from Madrid to other areas. CRF02_AG sequences from Hispanics were clustered in a separate subclade suggesting no linkage between the local and Hispanic subepidemics

La experiencia del Hospital Clínic de Barcelona: integración Facultad de Medicina - IDIBAPS - Hospital Universitario The experience of the Hospital Clinic of Barcelona: Integration Medical School-IDIBAPS- University Hospital

La investigación traslacional es una nueva disciplina que incorpora aspectos de ciencia básica e investigación clínica y, por tanto, requiere entrenamiento y recursos que no se suelen encontrar en los laboratorios o en departamentos clínicos. Es por ello que los institutos donde se combinan ambos tipos de investigación como universidades, centros de investigación básica y hospitales de alto nivel asistencial, son las instituciones ideales para realizar este tipo de investigación. En el Hospital Clínic de Barcelona se ha producido un cambio en el transcurso de los últimos 40 años. Este cambio ha venido marcado por varios hechos, a destacar la creación de los servicios clínicos que substituyeron a las antiguas cátedras, la creación de los servicios de medicina, las becas post-residencia, los años sabáticos, la Fundación Clínic para la Investigación Biomédica y finalmente la puesta en marcha del Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS). En los últimos años es importante destacar también la reorganización del hospital en institutos y centros. La producción científica en estos últimos años se ha mantenido en alza, tanto en número de publicaciones como del impacto de las revistas en las que se publica. El IDIBAPS ha alcanzado unos resultados similares a los obtenidos por los mejores institutos de investigación del mundo occidental.Translational research is a new discipline which incorporates aspects from both basic science and clinical investigation. As a result it requires training and resources which are not always available in laboratories or clinical departments. For this reason institutions which combine both clinical and basic research, such as universities, basic science centres and major hospitals, are the ideal institutions to perform this type of research. Over the past 40 years, Hospital Clinic in Barcelona, have been significant with the most marked changes in the following areas; the creation of clinical units which replaced the former cathedrae, the availability of grants for post residents, sabbaticals, the Foundation Clinic for Biomedical Investigation and finally the inauguration of IDIBAPS, Institut d'investigacions Biomediques August Pi y Sunyer. During more recent years the hospital has undergone major re-organization dividing it into Institutes and Centres. The scientific production of the past few years has continued to grow, not just in the number of articles published but also the number of articles published in high ranking Journals. Results show that IDIBAPS equals some of the best research and investigational institutions in the western world