4,757 research outputs found
Incipient plasticity and fully plastic contact behavior of copper coated with a graphene layer
Cu coated with a graphene layer increases the elastic modulus from 163.4 GPa to 176.7 GPa, as analyzed for the initial elastic loading during nanoindentation by the Hertzian contact theory. This is attributed to stiffening, due to the ultra-high elastic modulus of the graphene layer, and the compressive in-plane residual stresses in the Cu surface volume introduced by the lattice mismatch between graphene and Cu. The graphene layer induces incipient plasticity, manifested by pop-in events during nanoindentation loading, at shallower indentation depths. This could be due to the compressive in-plane residual stress in the Cu surface volume; however, this compressive stress does not significantly change the critical resolved shear stress for the incipient plasticity. Even in the fully plastic contact region, at an indentation depth of 100 nm, the graphene layer affects the stress distribution underneath the indenter, resulting in a lower pile-up height. When considering this reduced pile-up height, the graphene layer is found to enhance elastic modulus by 5%, whereas it has no effect on hardness
NELFE-Dependent MYC Signature Identifies a Unique Cancer Subtype in Hepatocellular Carcinoma.
The MYC oncogene is dysregulated in approximately 30% of liver cancer. In an effort to exploit MYC as a therapeutic target, including in hepatocellular carcinoma (HCC), strategies have been developed on the basis of MYC amplification or gene translocation. Due to the failure of these strategies to provide accurate diagnostics and prognostic value, we have developed a Negative Elongation Factor E (NELFE)-Dependent MYC Target (NDMT) gene signature. This signature, which consists of genes regulated by MYC and NELFE, an RNA binding protein that enhances MYC-induced hepatocarcinogenesis, is predictive of NELFE/MYC-driven tumors that would otherwise not be identified by gene amplification or translocation alone. We demonstrate the utility of the NDMT gene signature to predict a unique subtype of HCC, which is associated with a poor prognosis in three independent cohorts encompassing diverse etiologies, demographics, and viral status. The application of gene signatures, such as the NDMT signature, offers patients access to personalized risk assessments, which may be utilized to direct future care
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Expression of Heterologous OsDHAR Gene Improves Glutathione (GSH)-Dependent Antioxidant System and Maintenance of Cellular Redox Status in Synechococcus elongatus PCC 7942.
An excess of reactive oxygen species (ROS) can cause severe oxidative damage to cellular components in photosynthetic cells. Antioxidant systems, such as the glutathione (GSH) pools, regulate redox status in cells to guard against such damage. Dehydroascorbate reductase (DHAR, EC 1.8.5.1) catalyzes the glutathione-dependent reduction of oxidized ascorbate (dehydroascorbate) and contains a redox active site and glutathione binding-site. The DHAR gene is important in biological and abiotic stress responses involving reduction of the oxidative damage caused by ROS. In this study, transgenic Synechococcus elongatus PCC 7942 (TA) was constructed by cloning the Oryza sativa L. japonica DHAR (OsDHAR) gene controlled by an isopropyl β-D-1-thiogalactopyranoside (IPTG)-inducible promoter (Ptrc) into the cyanobacterium to study the functional activities of OsDHAR under oxidative stress caused by hydrogen peroxide exposure. OsDHAR expression increased the growth of S. elongatus PCC 7942 under oxidative stress by reducing the levels of hydroperoxides and malondialdehyde (MDA) and mitigating the loss of chlorophyll. DHAR and glutathione S-transferase activity were higher than in the wild-type S. elongatus PCC 7942 (WT). Additionally, overexpression of OsDHAR in S. elongatus PCC 7942 greatly increased the glutathione (GSH)/glutathione disulfide (GSSG) ratio in the presence or absence of hydrogen peroxide. These results strongly suggest that DHAR attenuates deleterious oxidative effects via the glutathione (GSH)-dependent antioxidant system in cyanobacterial cells. The expression of heterologous OsDHAR in S. elongatus PCC 7942 protected cells from oxidative damage through a GSH-dependent antioxidant system via GSH-dependent reactions at the redox active site and GSH binding site residues during oxidative stress
Magneto Transport of high TCR (temperature coefficient of resistance) La2/3Ca1/3MnO3: Ag Polycrystalline Composites
We report the synthesis, (micro)structural, magneto-transport and
magnetization of polycrystalline La2/3Ca1/3MnO3:Agx composites with x = 0.0,
0.1, 0.2, 0.3 and 0.4. The temperature coefficient of resistance (TCR) near
ferromagnetic (FM) transition is increased significantly with addition of Ag.
The FM transition temperature (TFM) is also increased slightly with Ag
addition. Magneto-transport measurements revealed that magneto-resistance MR is
found to be maximum near TFM. Further the increased MR of up to 60% is seen
above 300 K for higher silver added samples in an applied field of 7 Tesla.
Sharp TCR is seen near TFM with highest value of up to 15 % for Ag (0.4)
sample, which is an order of magnitude higher than as for present pristine
sample and best value yet reported for any polycrystalline LCMO compound.
Increased TCR, TFM and significant above room temperature MR of
La2/3Ca1/3MnO3:Agx composites is explained on the basis of improved grains size
and connectivity with silver addition in the matrix. Better coupled FM domains
and nearly conducting grain boundaries give rise to improved physical
properties of the La2/3Ca1/3MnO3 manganites.Comment: 16 pages Text + Figs. ACCEPTED: Solid State Communications (Sept.
2006
Exact soluble two-dimensional charged wormhole
We present an exactly soluble charged wormhole model in two dimensions by
adding infalling chiral fermions on the static wormhole. The infalling energy
due to the infalling charged matter requires the classical back reaction of the
geometry, which is solved by taking into account of the nontrivial nonchiral
exotic energy. Finally, we obtain the exact expression for the size of the
throat depending on the total amount of the infalling net energy and discuss
the interesting transition from the AdS spacetime to the wormhole geometry.Comment: 8 pages, no figure
ProNGF Induces p75-Mediated Death of Oligodendrocytes following Spinal Cord Injury
AbstractThe neurotrophin receptor p75 is induced by various injuries to the nervous system, but its role after injury has remained unclear. Here, we report that p75 is required for the death of oligodendrocytes following spinal cord injury, and its action is mediated mainly by proNGF. Oligodendrocytes undergoing apoptosis expressed p75, and the absence of p75 resulted in a decrease in the number of apoptotic oligodendrocytes and increased survival of oligodendrocytes. ProNGF is likely responsible for activating p75 in vivo, since the proNGF from the injured spinal cord induced apoptosis among p75+/+, but not among p75−/−, oligodendrocytes in culture, and its action was blocked by proNGF-specific antibody. Together, these data suggest that the role of proNGF is to eliminate damaged cells by activating the apoptotic machinery of p75 after injury
Homologous recombination DNA repair defects in PALB2-associated breast cancers
Mono-allelic germline pathogenic variants in the Partner And Localizer of BRCA2 (PALB2) gene predispose to a high-risk of breast
cancer development, consistent with the role of PALB2 in homologous recombination (HR) DNA repair. Here, we sought to define
the repertoire of somatic genetic alterations in PALB2-associated breast cancers (BCs), and whether PALB2-associated BCs display biallelic
inactivation of PALB2 and/or genomic features of HR-deficiency (HRD). Twenty-four breast cancer patients with pathogenic
PALB2 germline mutations were analyzed by whole-exome sequencing (WES, n = 16) or targeted capture massively parallel
sequencing (410 cancer genes, n = 8). Somatic genetic alterations, loss of heterozygosity (LOH) of the PALB2 wild-type allele, largescale
state transitions (LSTs) and mutational signatures were defined. PALB2-associated BCs were found to be heterogeneous at the
genetic level, with PIK3CA (29%), PALB2 (21%), TP53 (21%), and NOTCH3 (17%) being the genes most frequently affected by somatic
mutations. Bi-allelic PALB2 inactivation was found in 16 of the 24 cases (67%), either through LOH (n = 11) or second somatic
mutations (n = 5) of the wild-type allele. High LST scores were found in all 12 PALB2-associated BCs with bi-allelic PALB2 inactivation
sequenced by WES, of which eight displayed the HRD-related mutational signature 3. In addition, bi-allelic inactivation of PALB2 was
significantly associated with high LST scores. Our findings suggest that the identification of bi-allelic PALB2 inactivation in PALB2-
associated BCs is required for the personalization of HR-directed therapies, such as platinum salts and/or PARP inhibitors, as the vast
majority of PALB2-associated BCs without PALB2 bi-allelic inactivation lack genomic features of HRD.. The
authors thank Heather Thorne, Eveline Niedermayr, all the kConFab research nurses
and staff, the heads and staff of the Family Cancer Clinics, and the Clinical Follow Up
Study (which has received funding from the NHMRC, the National Breast Cancer
Foundation, Cancer Australia, and the National Institute of Health (USA)) for their
contributions to this resource, and the many families who contribute to kConFab.
Research reported in this paper was supported in part by the Breast Cancer Research
Foundation and the Sarah Jenkins Fund, a Cancer Center Support Grant of the
National Institutes of Health/National Cancer Institute (grant No. P30CA008748; MSK),
a grant of the Ministry of Health of the Czech Republic (NV15-29959A), Charles
University projects PROGRES Q28/LF1 and SVV2019/260367, an HIR Grant UM.C/HlR/
MOHE/06 from the Ministry of Higher Education, Malaysia, and the National Health
and Medical Research Council, Australia (NHMRC, Project Grant APP1029974).
kConFab is supported by a grant from the National Breast Cancer Foundation, and
previously by the NHMRC, the Queensland Cancer Fund, the Cancer Councils of New
South Wales, Victoria, Tasmania, and South Australia, and the Cancer Foundation of
Western Australia. W.D.F. was funded in part by Susan G Komen. A.L. was supported
by the China Scholarship Council. T.N.-D. is an Early Career Fellow of the National
Breast Cancer Foundation and M.S. is a NHMRC Senior Research Fellow of the
National Health and Medical Research Council. M.T. was funded by the National
Institute for Health Research (NIHR) Cambridge Biomedical Research Centre,
Addenbrooke’s Hospital and European Union Seventh Framework Program
(2007–2013)/European Research Council (310018). S.P. was supported by the Swiss
National Science Foundation (Ambizione grant number: PZ00P3_168165). J.S.R-F. is
partly funded by the Breast Cancer Research Foundation and Britta Weigelt by Cycle
for Survival
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