64 research outputs found
Developmental Validation of Short Tandem Repeat Reagent Kit for Forensic DNA Profiling of Canine Biological Material
Aim To develop a reagent kit that enables multiplex polymerase
chain reaction (PCR) amplification of 18 short tandem
repeats (STR) and the canine sex-determining Zinc
Finger marker.
Methods Validation studies to determine the robustness
and reliability in forensic DNA typing of this multiplex
assay included sensitivity testing, reproducibility studies,
intra- and inter-locus color balance studies, annealing
temperature and cycle number studies, peak height
ratio determination, characterization of artifacts such as
stutter percentages and dye blobs, mixture analyses, species-
specificity, case type samples analyses and population
studies.
Results The kit robustly amplified domesticated dog samples
and consistently generated full 19-locus profiles from
as little as 125 pg of dog DNA. In addition, wolf DNA samples
could be analyzed with the kit.
Conclusion The kit, which produces robust, reliable, and
reproducible results, will be made available for the forensic
research community after modifications based on this
study’s evaluation to comply with the quality standards expected
for forensic casework
Developmental Validation of Short Tandem Repeat Reagent Kit for Forensic DNA Profiling of Canine Biological Material
Aim To develop a reagent kit that enables multiplex polymerase
chain reaction (PCR) amplification of 18 short tandem
repeats (STR) and the canine sex-determining Zinc
Finger marker.
Methods Validation studies to determine the robustness
and reliability in forensic DNA typing of this multiplex
assay included sensitivity testing, reproducibility studies,
intra- and inter-locus color balance studies, annealing
temperature and cycle number studies, peak height
ratio determination, characterization of artifacts such as
stutter percentages and dye blobs, mixture analyses, species-
specificity, case type samples analyses and population
studies.
Results The kit robustly amplified domesticated dog samples
and consistently generated full 19-locus profiles from
as little as 125 pg of dog DNA. In addition, wolf DNA samples
could be analyzed with the kit.
Conclusion The kit, which produces robust, reliable, and
reproducible results, will be made available for the forensic
research community after modifications based on this
study’s evaluation to comply with the quality standards expected
for forensic casework
Galaxy clusters discovered with a Sunyaev-Zel'dovich effect survey
The South Pole Telescope (SPT) is conducting a Sunyaev-Zel'dovich (SZ) effect
survey over large areas of the southern sky, searching for massive galaxy
clusters to high redshift. In this preliminary study, we focus on a 40
square-degree area targeted by the Blanco Cosmology Survey (BCS), which is
centered roughly at right ascension 5h30m, declination -53 degrees. Over two
seasons of observations, this entire region has been mapped by the SPT at 95
GHz, 150 GHz, and 225 GHz. We report the four most significant SPT detections
of SZ clusters in this field, three of which were previously unknown and,
therefore, represent the first galaxy clusters discovered with an SZ survey.
The SZ clusters are detected as decrements with greater than 5-sigma
significance in the high-sensitivity 150 GHz SPT map. The SZ spectrum of these
sources is confirmed by detections of decrements at the corresponding locations
in the 95 GHz SPT map and non-detections at those locations in the 225 GHz SPT
map. Multiband optical images from the BCS survey demonstrate significant
concentrations of similarly colored galaxies at the positions of the SZ
detections. Photometric redshift estimates from the BCS data indicate that two
of the clusters lie at moderate redshift (z ~ 0.4) and two at high redshift (z
>~ 0.8). One of the SZ detections was previously identified as a galaxy cluster
using X-ray data from the ROSAT All-Sky Survey (RASS). Potential RASS
counterparts (not previously identified as clusters) are also found for two of
the new discoveries. These first four galaxy clusters are the most significant
SZ detections from a subset of the ongoing SPT survey. As such, they serve as a
demonstration that SZ surveys, and the SPT in particular, can be an effective
means for finding galaxy clusters.Comment: 11 pages, 3 figures, revised to match published version, uses
emulateap
The 10 Meter South Pole Telescope
The South Pole Telescope (SPT) is a 10 m diameter, wide-field, offset
Gregorian telescope with a 966-pixel, multi-color, millimeter-wave, bolometer
camera. It is located at the Amundsen-Scott South Pole station in Antarctica.
The design of the SPT emphasizes careful control of spillover and scattering,
to minimize noise and false signals due to ground pickup. The key initial
project is a large-area survey at wavelengths of 3, 2 and 1.3 mm, to detect
clusters of galaxies via the Sunyaev-Zeldovich effect and to measure the
small-scale angular power spectrum of the cosmic microwave background (CMB).
The data will be used to characterize the primordial matter power spectrum and
to place constraints on the equation of state of dark energy. A
second-generation camera will measure the polarization of the CMB, potentially
leading to constraints on the neutrino mass and the energy scale of inflation.Comment: 47 pages, 14 figures, updated to match version to be published in
PASP 123 903 (May, 2011
Sunyaev-Zel'dovich Cluster Profiles Measured with the South Pole Telescope
We present Sunyaev-Zel'dovich measurements of 15 massive X-ray selected
galaxy clusters obtained with the South Pole Telescope. The Sunyaev-Zel'dovich
(SZ) cluster signals are measured at 150 GHz, and concurrent 220 GHz data are
used to reduce astrophysical contamination. Radial profiles are computed using
a technique that takes into account the effects of the beams and filtering. In
several clusters, significant SZ decrements are detected out to a substantial
fraction of the virial radius. The profiles are fit to the beta model and to a
generalized NFW pressure profile, and are scaled and stacked to probe their
average behavior. We find model parameters that are consistent with previous
studies: beta=0.86 and r_core/r_500 = 0.20 for the beta model, and (alpha,
beta, gamma, c_500)=(1.0,5.5,0.5,1.0) for the generalized NFW model. Both
models fit the SPT data comparably well, and both are consistent with the
average SZ profile out to the virial radius. The integrated Compton-y parameter
Y_SZ is computed for each cluster using both model-dependent and
model-independent techniques, and the results are compared to X-ray estimates
of cluster parameters. We find that Y_SZ scales with Y_X and gas mass with low
scatter. Since these observables have been found to scale with total mass, our
results point to a tight mass-observable relation for the SPT cluster survey.Comment: 21 pages, 24 figures, updated to published versio
A Measurement of the Cosmic Microwave Background Damping Tail from the 2500-square-degree SPT-SZ survey
We present a measurement of the cosmic microwave background (CMB) temperature
power spectrum using data from the recently completed South Pole Telescope
Sunyaev-Zel'dovich (SPT-SZ) survey. This measurement is made from observations
of 2540 deg of sky with arcminute resolution at GHz, and improves
upon previous measurements using the SPT by tripling the sky area. We report
CMB temperature anisotropy power over the multipole range . We
fit the SPT bandpowers, combined with the seven-year Wilkinson Microwave
Anisotropy Probe (WMAP7) data, with a six-parameter LCDM cosmological model and
find that the two datasets are consistent and well fit by the model. Adding SPT
measurements significantly improves LCDM parameter constraints; in particular,
the constraint on tightens by a factor of 2.7. The impact of
gravitational lensing is detected at , the most significant
detection to date. This sensitivity of the SPT+WMAP7 data to lensing by
large-scale structure at low redshifts allows us to constrain the mean
curvature of the observable universe with CMB data alone to be
. Using the SPT+WMAP7 data, we measure the
spectral index of scalar fluctuations to be in the LCDM
model, a preference for a scale-dependent spectrum with .
The SPT measurement of the CMB damping tail helps break the degeneracy that
exists between the tensor-to-scalar ratio and in large-scale CMB
measurements, leading to an upper limit of (95%,C.L.) in the LCDM+
model. Adding low-redshift measurements of the Hubble constant () and the
baryon acoustic oscillation (BAO) feature to the SPT+WMAP7 data leads to
further improvements. The combination of SPT+WMAP7++BAO constrains
in the LCDM model, a detection of , ... [abridged]Comment: 21 pages, 10 figures. Replaced with version accepted by ApJ. Data
products are available at http://pole.uchicago.edu/public/data/story12
A Measurement of the Correlation of Galaxy Surveys with CMB Lensing Convergence Maps from the South Pole Telescope
We compare cosmic microwave background lensing convergence maps derived from South Pole Telescope (SPT) data with galaxy survey data from the Blanco Cosmology Survey, WISE, and a new large Spitzer/IRAC field designed to overlap with the SPT survey. Using optical and infrared catalogs covering between 17 and 68 deg^2 of sky, we detect a correlation between the SPT convergence maps and each of the galaxy density maps at >4σ, with zero correlation robustly ruled out in all cases. The amplitude and shape of the cross-power spectra are in good agreement with theoretical expectations and the measured galaxy bias is consistent with previous work. The detections reported here utilize a small fraction of the full 2500 deg^2 SPT survey data and serve as both a proof of principle of the technique and an illustration of the potential of this emerging cosmological probe
South Pole Telescope Detections of the Previously Unconfirmed Planck Early SZ Clusters in the Southern Hemisphere
We present South Pole Telescope (SPT) observations of the five galaxy cluster
candidates in the southern hemisphere which were reported as unconfirmed in the
Planck Early Sunyaev-Zel'dovich (ESZ) sample. One cluster candidate, PLCKESZ
G255.62-46.16, is located in the 2500-square-degree SPT SZ survey region and
was reported previously as SPT-CL J0411-4819. For the remaining four
candidates, which are located outside of the SPT SZ survey region, we performed
short, dedicated SPT observations. Each of these four candidates was strongly
detected in maps made from these observations, with signal-to-noise ratios
ranging from 6.3 to 13.8. We have observed these four candidates on the
Magellan-Baade telescope and used these data to estimate cluster redshifts from
the red sequence. Resulting redshifts range from 0.24 to 0.46. We report
measurements of Y_0.75', the integrated Comptonization within a 0.75' radius,
for all five candidates. We also report X-ray luminosities calculated from
ROSAT All-Sky Survey catalog counts, as well as optical and improved SZ
coordinates for each candidate. The combination of SPT SZ measurements, optical
red-sequence measurements, and X-ray luminosity estimates demonstrates that
these five Planck ESZ cluster candidates do indeed correspond to real galaxy
clusters with redshifts and observable properties consistent with the rest of
the ESZ sample.Comment: 7 emulateapj pages, 4 figures, 1 table. Revised to match published
versio
Measurements of Secondary Cosmic Microwave Background Anisotropies with the South Pole Telescope
We report cosmic microwave background (CMB) power spectrum measurements from
the first 100 sq. deg. field observed by the South Pole Telescope (SPT) at 150
and 220 GHz. On angular scales where the primary CMB anisotropy is dominant,
ell ~< 3000, the SPT power spectrum is consistent with the standard LambdaCDM
cosmology. On smaller scales, we see strong evidence for a point source
contribution, consistent with a population of dusty, star-forming galaxies.
After we mask bright point sources, anisotropy power on angular scales of 3000
50 at both frequencies. We
combine the 150 and 220 GHz data to remove the majority of the point source
power, and use the point source subtracted spectrum to detect
Sunyaev-Zel'dovich (SZ) power at 2.6 sigma. At ell=3000, the SZ power in the
subtracted bandpowers is 4.2 +/- 1.5 uK^2, which is significantly lower than
the power predicted by a fiducial model using WMAP5 cosmological parameters.
This discrepancy may suggest that contemporary galaxy cluster models
overestimate the thermal pressure of intracluster gas. Alternatively, this
result can be interpreted as evidence for lower values of sigma8. When combined
with an estimate of the kinetic SZ contribution, the measured SZ amplitude
shifts sigma8 from the primary CMB anisotropy derived constraint of 0.794 +/-
0.028 down to 0.773 +/- 0.025. The uncertainty in the constraint on sigma8 from
this analysis is dominated by uncertainties in the theoretical modeling
required to predict the amplitude of the SZ power spectrum for a given set of
cosmological parameters.Comment: 28 pages, 11 figures, submitted to Ap
Angular Power Spectra of the Millimeter Wavelength Background Light from Dusty Star-forming Galaxies with the South Pole Telescope
We use data from the first 100 square-degree field observed by the South Pole
Telescope (SPT) in 2008 to measure the angular power spectrum of temperature
anisotropies contributed by the background of dusty star-forming galaxies
(DSFGs) at millimeter wavelengths. From the auto and cross-correlation of 150
and 220 GHz SPT maps, we significantly detect both Poisson distributed and, for
the first time at millimeter wavelengths, clustered components of power from a
background of DSFGs. The spectral indices between 150 and 220 GHz of the
Poisson and clustered components are found to be 3.86 +- 0.23 and 3.8 +- 1.3
respectively, implying a steep scaling of the dust emissivity index beta ~ 2.
The Poisson and clustered power detected in SPT, BLAST (at 600, 860, and 1200
GHz), and Spitzer (1900 GHz) data can be understood in the context of a simple
model in which all galaxies have the same graybody spectrum with dust
emissivity index of beta = 2 and dust temperature T_d = 34 K. In this model,
half of the 150 GHz background light comes from redshifts greater than 3.2. We
also use the SPT data to place an upper limit on the amplitude of the kinetic
Sunyaev-Zel'dovich power spectrum at l = 3000 of 13 uK^2 at 95% confidence.Comment: 18 pages, 9 figure
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