Consensus Recommendation for Mouse Models of Ocular Hypertension to Study Aqueous Humor Outflow and Its Mechanisms.

Due to their similarities in anatomy, physiology, and pharmacology to humans, mice are a valuable model system to study the generation and mechanisms modulating conventional outflow resistance and thus intraocular pressure. In addition, mouse models are critical for understanding the complex nature of conventional outflow homeostasis and dysfunction that results in ocular hypertension. In this review, we describe a set of minimum acceptable standards for developing, characterizing, and utilizing mouse models of open-angle ocular hypertension. We expect that this set of standard practices will increase scientific rigor when using mouse models and will better enable researchers to replicate and build upon previous findings

Quantitative proteomic identification of host factors involved in the Salmonella typhimurium infection cycle

Phone +31(0)302535375, Fax +31(0)302535492 Abbreviations: cfu, colony forming unit; ERBB2IP, erbin interacting protein; ITGB4, isoform beta-4c of integrin beta-4; MOI, multiplicity of infection; p.i., post infection; PNS, post nuclear supernatant; PRDX6, peroxiredoxin 6; RT-PCR, real-time PCR; SCV, Salmonella-containing vacuole; siRNA, small interfering RNA; SPI-1, Salmonella pathogenicity island 1; SPI-2, Salmonella pathogenicity island 2; STOM, stomatin; T3SS, type three secretion system; TBC1D10B, TBC domain containing protein Surprisingly, in addition to the effect on Salmonella replication, depletion of STOM or ITGB4 resulted in a dispersal of intracellular Salmonella microcolonies. It can be concluded that by using SILAC-based quantitative proteomics we were able to identify novel host cell proteins involved in the complex interplay between Salmonella and epithelial cells