HUBUNGAN ANTARA BEBAN KERJA DAN KEPUASAN KERJA DENGAN STRES KERJA PADA PERAWAT PELAKSANA DIRUMAH SAKIT UMUM DAERAH AMURANG

Stres kerja merupakan hal yang sangat menggangu pekerjaan. Terjadinya stres akibat kerja secara khusus akan dapat menurunkan produktivitas kerja antara lain performansi pekerja yang rendah, meningkatnya angka absensi, menurunnya moral kerja meningkatnya turnover pekerja yang dapat menyebabkan kehilangan banyak waktu kerja menyebabkan biaya kompensasi pekerja meningkat. Jika secara emosional pekerja merasa puas berarti kepuasan kerja tercapai sebaliknya jika tidak maka berarti karyawan tidak puas. Penelitian ini bertujuan untuk mengetahui hubungan antara beban kerja dan kepuasan kerja dengan stres kerja pada perawat di Rumah Sakit Umum Daerah Amurang. Metode penelitian adalah penelitian kuantitatif, menggunakan metode survei analitik dengan pendekatan cross sectional study pada perawat pelaksana Diruang Rawat Inap dan Instalasi Gawat Darurat dirumah Sakit Umum Daerah Amurang dan waktu penelitian ini akan dilaksanakan pada bulan Oktober-Desember 2019.Hasil penelitian yang dilakukan  dengan penggukuran menggunakan kuesioner dan dianalisis menggunakan Chi Square menunjukan dari 41 responden ada 40 yang mengalami beban kerja berat, kemudian berdasarkan kepuasan kerja ada 36 reponden yang tidak puas, sedangkan responden yang mengalami stres kerja ada 37 dari 41 responden. Kata kunci: Beban Kerja, Kepuasan Kerja, Stres Kerja, RSUD Amurang ABSTRACTJob stress is a very disturbing thing to work. The occurrence of stress due to work specifically will be able to reduce work productivity, among others, low employee performance, increased absenteeism, decreased work morale, increased employee turnover which can cause a lot of loss of work time causing workers compensation costs to increase. If emotionally satisfied workers mean job satisfaction is achieved otherwise if it does not mean that employees are not satisfied. This study aims to determine the relationship between workload and job satisfaction with work stress on nurses at Amurang District General Hospital. The research method is quantitative research, using analytic survey method with cross-sectional study approach to the nurses implementing the Inpatient and Emergency Room at the Amurang Regional General Hospital and the time of this research will be carried out in October-December 2019. The results of research conducted by shaving using questionnaires and analyzed using Chi-Square showed that of 41 respondents there were 40 who experienced heavy workloads, then based on job satisfaction there were 36 respondents who were dissatisfied, while respondents who experienced work stress were 37 out of 41 respondents. Keywords : Workload, Job Satisfaction, Job Stress, Amurang Regional Hospita

HUBUNGAN ANTARA TINDAKAN TIDAK AMAN DENGAN KECELAKAAN KERJA PADA TENAGA KERJA BONGKAR MUAT DI PT PELABUHAN INDONESIA IV (PERSERO) CABANG BITUNG

Kecelakaan disebabkan oleh dua hal pokok yaitu tindakan tidak aman dan kondisi tidak aman (Depkes, 2014). Tindakan tidak aman adalah suatu kegagalan dalam mengikuti persyaratan dan prosedur-prosedur kerja yang benar sehingga dapat menyebabkan terjadinya kecelakaan kerja . Dengan tujuan penelitian untuk mengetahui hubungan antara antara tindakan tidak aman dengan kecelakaan kerja pada tenaga kerja bongkar muat di PT. Pelabuhan Indonesia IV (Persero) Cabang Bitung. Penelitian ini merupakan penelitian survei analitik dengan metode cross sectional study. Populasi berjumlah 914 responden dengan sampel 100 responden diperoleh secara simple random sampling. Variabel penelitian diukur dengan menggunakan kuesioner. Hubungan antara variabel ditentukan menggunakan uji statistik fisher exact test dengan nilai a = 0,05 dan CI = 90%. Hasil probabilitas antara tindakan tidak aman dengan kecelakaan kerja dengan nilai p= 1,000. Tindakan tidak aman (26%) dan kecelakaan kerja (19%). Hasil penelitian menunjukkan bahwa tidak terdapat hubungan antara tindakan tidak aman dengan kecelakaan kerja pada tenaga kerja bongkar muat di PT. Pelabuhan Indonesia IV (Persero) Cabang Bitung.Kata Kunci: Tindakan tidak aman, kecelakaan kerjaABSTRACTAn accident caused by two things of the principal amount of pt pgn promised to supply the action is does not security and the reality of it is unsafe action ( Depkes , 2014 ) .The action is does not the safe is a failure in participating in the requirements and procedure-procedure the right work so as to cause the occurrence of the facility of employment accident. Research purposes to know the relationship between between unsafe action by accident work in labor unloading PT. Pelabuhan Indonesia IV ( Persero ) branches Bitung . The research is the survey research analytic with the methods cross sectional study .The population were 914 respondents were selected from 100 respondents obtained in simple random sampling .Research variables measured using a questionnaire .The relation between variables specified using exact statistical testing fisher test with a value of a = 0.05 and CI = 90 %. The results of the probability between unsafe action by accident working with value p = 1,000.The act of unsafe ( 26 % ) and accidents ( 19 % ). The research results show that there was no correlation between unsafe action by accident work in labor loading and unloading at PT .Pelabuhan Indonesia IV ( Persero ) the Branch BitungKeywords: unsafe action, work acciden

HUBUNGAN ANTARA UMUR DAN MASA KERJA DENGAN KELUHAN MUSCULOSKELETAL PADA KUSIR BENDI DI KOTA TOMOHON

Bendi merupakan salah satu alat transportasi yang masih aktif beroperasi di kota Tomohon dengan jumlah keseluruhan yaitu sebanyak 40 orang. Pekerjaan yang dilakukan oleh kusir bendi di Kota Tomohon memiliki risiko seperti keluhan mulai dari leher, punggung atas, pinggang dan tangan. Tujuan penelitian ini untuk menganalisis hubungan antara umur dan masa kerja dengan keluhan Musculoskeletal pada kusir bendi di kota Tomohon. Jenis penelitian yang digunakan adalah observasional analitik dengan pendekatan cross sectional. Populasi dalam penelitian ini adalah kusir bendi di kota Tomohon yang berjumlah 40 orang berdasarkan total populasi perkumpulan kusir bendi di KotaTomohon. Pengumpulan data menggunakan kuesinoner, keluhan Musculoskeletal menggunakan Nordic Body Map (NBM). Hasil uji korelasi dengan uji statistic spearman antara umur dengan keluhan musculoskeletal p value=0,04 < 0,05, dan keofisien korelasi r=0,327 atau korelasi sedang dengan arah hubungan positif (+) dan masa kerja dengan keluhan musculoskeletal p value=0,015 < 0,05 dan keofisien korelasi r=0,383 atau korelasi sedang dengan arah hubungan positif (+). Kesimpulanya bahwa terdapat hubungan antara umur dan masa kerja dengan keluhan musculoskeletal pada kusir bendi dengan kekuatan korelasi kuat dan arah korelasi positif (+) atau searah yang berarti semakin bertambahnya usia seseorang maka akan semakin meningkat keluhan musculoskeletal dan semakin tinggi masa kerja seseorang maka akan semakin besar dampak gangguan keluhan musculoskeletal.Kata Kunci: Umur, Masa Kerja, Keluhan Musculoskeletal, dan Kusir BendiABSTRACTThe buggy is one means of transportation were still active in Tomohon city with a total of as many as 40. Work carried out by the coachman gig in Tomohon have risks such as complaints from the neck, upper back, hips and hands. The purpose of this study to analyze the relationship between age and years of service with the coachman gig Musculoskeletal complaints in the town of Tomohon. This type of research is observational analytic with cross sectional approach. The population in this study was the coachman gig in Tomohon, amounting to 40 based on total population coachman gig in KotaTomohon association. Collecting data using kuesinoner, Musculoskeletal complaints using Nordic Body Map (NBM).The test results with statistical tests Spearman correlation between age and musculoskeletal complaints p value = 0.04 <0.05, and keofisien correlation of r = 0.327 or moderate correlation with the direction of the positive (+) and years of service with musculoskeletal complaints p value = 0.015 < 0.05 and keofisien correlation r = 0.383 or moderate correlation with the direction of the positive (+). Kesimpulanya that there is a relationship between age and length of service with complaints of musculoskeletal the coachman gig with the power of a strong correlation and direction of the correlation is positive (+) or unidirectional, which means the increasing age of a person it will be increasing complaints of musculoskeletal and higher service life of a person, the greater the impact of musculoskeletal complaints interference.Keywords: Age, Tenure, Musculoskeletal Complaints, and driver Bend

Coordinated RNA-Seq and peptidomics identify neuropeptides and G-protein coupled receptors (GPCRs) in the large pine weevil Hylobius abietis, a major forestry pest

Hylobius abietis (Linnaeus), or large pine weevil (Coleoptera, Curculionidae), is a pest of European coniferous forests. In order to gain understanding of the functional physiology of this species, we have assembled a de novo transcriptome of H. abietis, from sequence data obtained by Next Generation Sequencing. In particular, we have identified genes encoding neuropeptides, peptide hormones and their putative G-protein coupled receptors (GPCRs) to gain insights into neuropeptide-modulated processes. The transcriptome was assembled de novo from pooled paired-end, sequence reads obtained from RNA from whole adults, gut and central nervous system tissue samples. Data analysis was performed on the transcripts obtained from the assembly including, annotation, gene ontology and functional assignment as well as transcriptome completeness assessment and KEGG pathway analysis. Pipelines were created using Bioinformatics tools and techniques for prediction and identification of neuropeptides and neuropeptide receptors. Peptidomic analysis was also carried out using a combination of MALDI-TOF as well as Q-Exactive Orbitrap mass spectrometry to confirm the identified neuropeptide. 41 putative neuropeptide families were identified in H. abietis, including Adipokinetic hormone (AKH), CAPA and DH31. Neuropeptide F, which has not been yet identified in the model beetle T. castaneum, was identified. Additionally, 24 putative neuropeptide and 9 leucine-rich repeat containing G protein coupled receptor-encoding transcripts were determined using both alignment as well as non-alignment methods. This information, submitted to the NCBI sequence read archive repository (SRA accession: SRP133355), can now be used to inform understanding of neuropeptide-modulated physiology and behaviour in H. abietis; and to develop specific neuropeptide-based tools for H. abietis control

Temporal Stability of Surface Roughness Effects on Radar Based Soil Moisture Retrieval During the Corn Growth Cycle

A representative soil surface roughness parameterization needed for the retrieval of soil moisture from active microwave satellite observation is difficult to obtain through either in-situ measurements or remote sensing-based inversion techniques. Typically, for the retrieval of soil moisture, temporal variations in surface roughness are assumed to be negligible. Although previous investigations have suggested that this assumption might be reasonable for natural vegetation covers (Moran et al. 2002, Thoma et al. 2006), insitu measurements over plowed agricultural fields (Callens et al. 2006) have shown that the soil surface roughness can change considerably over time. This paper reports on the temporal stability of surface roughness effects on radar observations and soil moisture retrieved from these radar observations collected once a week during a corn growth cycle (May 10th - October 2002). The data set employed was collected during the Optimizing Production Inputs for Economic and Environmental Enhancement (OPE3) field campaign covering this 2002 corn growth cycle and consists of dual-polarized (HH and VV) L-band (1.6 GHz) acquired at view angles of 15, 35, and 55 degrees. Cross-polarized L baud radar data were also collected as part of this experiment, but are not used in the analysis reported on here. After accounting for vegetation effects on radar observations, time-invariant optimum roughness parameters were determined using the Integral Equation Method (IEM) and radar observations acquired over bare soil and cropped conditions (the complete radar data set includes entire corn growth cycle). The optimum roughness parameters, soil moisture retrieval uncertainty, temporal distribution of retrieval errors and its relationship with the weather conditions (e.g. rainfall and wind speed) have been analyzed. It is shown that over the corn growth cycle, temporal roughness variations due to weathering by rain are responsible for almost 50% of soil moisture retrieval uncertainty depending on the sensing configuration. The effects of surface roughness variations are found to be smallest for observations acquired at a view angle of 55 degrees and HH polarization. A possible explanation for this result is that at 55 degrees and HH polarization the effect of vertical surface height changes on the observed radar response are limited because the microwaves travel parallel to the incident plane and as a result will not interact directly with vertically oriented soil structures

Simultaneous measurement of flight time and energy of large matrix-assisted laser desorption ionization ions with a superconducting tunnel junction detector

We evaluated a cryogenically cooled superconducting Nb-Al2O3-Nb tunnel junction (STJ) for use as a molecular ion detector in a matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometer. The STJ responds to ion energy and theoretically should detect large molecular ions with a velocity-independent efficiency approaching 100%. The STJ detector produces pulses whose heights are approximately proportional to ion energy, thus the height of a pulse generated by the impact of a doubly charged ion is about twice the height of a singly charged ion pulse. Measurements were performed by bombarding the STJ with human serum albumin (HSA) (66,000 Da) and immunoglobulin (150,000 Da) ions. We demonstrate that pulse height analysis of STJ signals provides a way to distinguish with good discrimination HSA+ from 2HSA2+, whose flight times are coincident. The rise time of STJ detector pulses allows ion flight times to be determined with a precision better than 200 ns, which is a value smaller than the flight time variation typically observed for large isobaric MALDI ions detected with conventional microchannel plate (MCP) detectors. Deflection plates in the flight tube of the mass spectrometer provided a way to aim ions alternatively at a MCP ion detector

Prospects and constraints on utilization of Jatropha curcas seeds in animal feedstuff

Combined physical, chemical and biochemical methods were used to process virgin seed meal of Jatropha curcas L. into treated Jatropha Seed Meal (JSM). Graded levels (0, 5, 10, 15, 20 and 25%) JSM treated by the various methods was included in the test diets which were fed ad libitum to 360 albino rats for a month in a single factor design trial. The results revealed that feed intake was numerically highest (p &gt; 0.05) for the rats on the 5 % treated JSM diet, while feed efficiency was highest (p &gt; 0.05) for those on the 10 % treated JSM diets. Weight gain was significantly highest (p&lt; 0.05) for the rats on 10 and 15 % treated JSM diets. Lowest survival rate (17%) was observed on treated JSM diet processed by boiling, roasting followed by fermenting. Biochemical indices measured on treated JSM based diets were comparable with those obtained on the conventional diet (p &gt; 0.05) except for blood cholesterol level (p &lt; 0.05) which was elevated with increasing treated JSM in diets. There were also no significant differences recorded on AST and ALP activities (p &gt; 0.05) between the treatments. Haematological indices investigated including PCV, RBC, Hb were insignificantly influenced by the 5% dietary treated JSM compared with the control diet (p &gt; 0.05) and there was non-significant effect of the dietary treatment on the WBC differential counts of lymphocytes, eosinophils, monocytes and neutrophils. It was established in this study that inclusion of 5% treated JSM in diets had no detrimental effect on albino rats. Further researches are in progress to investigate inclusion of the treated JSM in diets at levels higher than the 5% used in this study.Keywords: JSM, Cockerels, Performance, Biochemical and haematological parameter

Rapid 13(c) urea breath test to identify Helicobacter pylori infection in emergency department patients with upper abdominal pain

Introduction: In emergency department (ED) patients with upper abdominal pain, management includes ruling out serious diseases and providing symptomatic relief. One of the major causes of upper abdominal pain is an ulcer caused by Helicobacter pylori (H. pylori), which can be treated and cured with antibiotics. We sought to estimate the prevalence of H. pylori infection in symptomatic patients using a convenience sample at a single urban academic ED and demonstrate the feasibility of ED-based testing. Methods: We prospectively enrolled patients with a chief complaint of pain or discomfort in the upper abdomen for 1 year from February 2011 until February 2012 at a single academic urban ED. Enrolled subjects were tested for H. pylori using a rapid point of care 13C Urea Breath Test (UBT) [Exalenz Bioscience]. We compared patient characteristics between those who tested positive versus negative for the disease. Results: A total of 205 patients with upper abdominal pain were tested over 12 months, and 24% (95% confidence interval: 19% to 30%) tested positive for H. pylori. Black subjects were more likely to test positive than white subjects (28% v. 6%, P \u3c 0.001). Other factors, such as age and sex, were not different between the 2 groups. Conclusion: In our ED, H. pylori infection was present in 1 in 4 patients with epigastric pain, and testing with a UBT was feasible. Further study is needed to determine the risk factors associated with infection, the prevalence of H. pylori in other EDs, the effect of the test on ED length of stay and the cost-effectiveness of an ED-based test-and-treat strategy. [West J Emerg Med. 2013;14(3):278–282.

Technical Note: Calibration and validation of geophysical observation models

We present a method to calibrate and validate observational models that interrelate remotely sensed energy fluxes to geophysical variables of land and water surfaces. Coincident sets of remote sensing observation of visible and microwave radiations and geophysical data are assembled and subdivided into calibration (Cal) and validation (Val) data sets. Each Cal/Val pair is used to derive the coefficients (from the Cal set) and the accuracy (from the Val set) of the observation model. Combining the results from all Cal/Val pairs provides probability distributions of the model coefficients and model errors. The method is generic and demonstrated using comprehensive matchup sets from two very different disciplines: soil moisture and water quality. The results demonstrate that the method provides robust model coefficients and quantitative measure of the model uncertainty. This approach can be adopted for the calibration/validation of satellite products of land and water surfaces, and the resulting uncertainty can be used as input to data assimilation schemes