Impact of COVID-19 on cardiovascular testing in the United States versus the rest of the world

Objectives: This study sought to quantify and compare the decline in volumes of cardiovascular procedures between the United States and non-US institutions during the early phase of the coronavirus disease-2019 (COVID-19) pandemic. Background: The COVID-19 pandemic has disrupted the care of many non-COVID-19 illnesses. Reductions in diagnostic cardiovascular testing around the world have led to concerns over the implications of reduced testing for cardiovascular disease (CVD) morbidity and mortality. Methods: Data were submitted to the INCAPS-COVID (International Atomic Energy Agency Non-Invasive Cardiology Protocols Study of COVID-19), a multinational registry comprising 909 institutions in 108 countries (including 155 facilities in 40 U.S. states), assessing the impact of the COVID-19 pandemic on volumes of diagnostic cardiovascular procedures. Data were obtained for April 2020 and compared with volumes of baseline procedures from March 2019. We compared laboratory characteristics, practices, and procedure volumes between U.S. and non-U.S. facilities and between U.S. geographic regions and identified factors associated with volume reduction in the United States. Results: Reductions in the volumes of procedures in the United States were similar to those in non-U.S. facilities (68% vs. 63%, respectively; p = 0.237), although U.S. facilities reported greater reductions in invasive coronary angiography (69% vs. 53%, respectively; p < 0.001). Significantly more U.S. facilities reported increased use of telehealth and patient screening measures than non-U.S. facilities, such as temperature checks, symptom screenings, and COVID-19 testing. Reductions in volumes of procedures differed between U.S. regions, with larger declines observed in the Northeast (76%) and Midwest (74%) than in the South (62%) and West (44%). Prevalence of COVID-19, staff redeployments, outpatient centers, and urban centers were associated with greater reductions in volume in U.S. facilities in a multivariable analysis. Conclusions: We observed marked reductions in U.S. cardiovascular testing in the early phase of the pandemic and significant variability between U.S. regions. The association between reductions of volumes and COVID-19 prevalence in the United States highlighted the need for proactive efforts to maintain access to cardiovascular testing in areas most affected by outbreaks of COVID-19 infection

Statement of Second Brazilian Congress of Mechanical Ventilarion : part I

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Effect of 8-methoxypsoralen plus near ultraviolet light on prophages and plasmid of Staphylococcus aureus

O presente trabalho teve como objetivo avaliar a 8-metoxipsoraleina associada à luz ultravioleta longa (8MOP-UVL) na indução de profagos, na cura do profago responsável pela supressão da atividade lipolítica e na cura do plasmídio responsável pela resistência à tetraciclina, na linhagem 609tet-r de Staphylococcus aureus. As frequências de indução de profagos, após irradiação por UVL, na presença de 8MOP, quando lançadas em gráfico, em função da dose, revelaram uma curva de dose-efeito típica, de há muito conhecida para outros sistemas lisogênicos. Para as condições experimentais utilizadas, a frequência máxima de indução foi maior que aquela obtida após irradiação por luz ultravioleta curta (UVC). Após irradiação por UVL, na presença de 8MOP, a partir da linhagem lip- utilizada, variantes lip+ foram obtidas com frequências maiores que as obtidas espontaneamente, sendo o tratamento tão eficiente quanto a UVC. Fagotipagem e experimentos de conversão fágica evidenciaram a ocorrência de cura do profago, detectando-se assim mais um efeito biológico da associação 8MOP-UVL em bactérias. Após tratamento com 8MOP-UVL, variantes sensíveis à tetraciclina foram obtidas com frequências maiores que as obtidas espontaneamente ou sob ação de temperatura elevada ou brometo de etídio. Com as variantes sensíveis e com a linhagem original, foi feita eletroforese de DNA em gel de agarose e o resultado mostrou que concomitante ao desaparecimento do caráter fenotípico, houve também desaparecimento de uma banda que pôde ser identificada como relativa ao plasmídio portador do gene para resistência a tetraciclina. Assim a associação 8MOP-UVL poderia ser considerada como um novo agente capaz de eliminar plasmídios de S. aureus.The present research was aimed to evaluate the association of 8-methoxypsoralen plus near ultraviolet light (8MOP-NUV) on induction of prophages and cure of the prophage responsible for suppression of the lypolitic activity on Staphylococcus aureus. The same association was also investigated on the cure of the plasmid responsible by tetracycline resistance on the used strain 609tet-r. The prophage induction frequencies after NUV irradiation associated to 8MOP treatment have shown in a graphic a typical dosis-effect curve also known for other lysogenic systems. For the experimental conditions the maximum frequency of induction was greater than that obtained after far ultraviolet light irradiation (FUV). After 8MOP-NUV treatment, variants lip+ were obtained from the lip- strain used, in frequencies which were greater than those achieved spontaneously. This treatment was as efficient as the FUV one. Bacteriophage typing and experiments of phage conversion have shown that cure of prophage was achieved. This can be considered one additional biological effect of 8MOP-NUV association. Following 8MOP-NUV treatment, variants which were tetracycline sensitive were obtained. The observed frequencies were higher than those achieved spontaneously or after ethidium bromide and high temperature treatments. Gel agarose electrophoresis were carried out from the original strain and non-resistant variants lysates. The results have shown that loss of resistance correspond to a loss of a band on agarose gel which must correspond to the plasmid carrying the tetracycline resistance gene. In this way 8MOP-NUV association can be considered as another agent able to eliminate plasmids from S. aureus

Effect of 8-methoxypsoralen plus near ultraviolet light on prophages and plasmid of Staphylococcus aureus

O presente trabalho teve como objetivo avaliar a 8-metoxipsoraleina associada à luz ultravioleta longa (8MOP-UVL) na indução de profagos, na cura do profago responsável pela supressão da atividade lipolítica e na cura do plasmídio responsável pela resistência à tetraciclina, na linhagem 609tet-r de Staphylococcus aureus. As frequências de indução de profagos, após irradiação por UVL, na presença de 8MOP, quando lançadas em gráfico, em função da dose, revelaram uma curva de dose-efeito típica, de há muito conhecida para outros sistemas lisogênicos. Para as condições experimentais utilizadas, a frequência máxima de indução foi maior que aquela obtida após irradiação por luz ultravioleta curta (UVC). Após irradiação por UVL, na presença de 8MOP, a partir da linhagem lip- utilizada, variantes lip+ foram obtidas com frequências maiores que as obtidas espontaneamente, sendo o tratamento tão eficiente quanto a UVC. Fagotipagem e experimentos de conversão fágica evidenciaram a ocorrência de cura do profago, detectando-se assim mais um efeito biológico da associação 8MOP-UVL em bactérias. Após tratamento com 8MOP-UVL, variantes sensíveis à tetraciclina foram obtidas com frequências maiores que as obtidas espontaneamente ou sob ação de temperatura elevada ou brometo de etídio. Com as variantes sensíveis e com a linhagem original, foi feita eletroforese de DNA em gel de agarose e o resultado mostrou que concomitante ao desaparecimento do caráter fenotípico, houve também desaparecimento de uma banda que pôde ser identificada como relativa ao plasmídio portador do gene para resistência a tetraciclina. Assim a associação 8MOP-UVL poderia ser considerada como um novo agente capaz de eliminar plasmídios de S. aureus.The present research was aimed to evaluate the association of 8-methoxypsoralen plus near ultraviolet light (8MOP-NUV) on induction of prophages and cure of the prophage responsible for suppression of the lypolitic activity on Staphylococcus aureus. The same association was also investigated on the cure of the plasmid responsible by tetracycline resistance on the used strain 609tet-r. The prophage induction frequencies after NUV irradiation associated to 8MOP treatment have shown in a graphic a typical dosis-effect curve also known for other lysogenic systems. For the experimental conditions the maximum frequency of induction was greater than that obtained after far ultraviolet light irradiation (FUV). After 8MOP-NUV treatment, variants lip+ were obtained from the lip- strain used, in frequencies which were greater than those achieved spontaneously. This treatment was as efficient as the FUV one. Bacteriophage typing and experiments of phage conversion have shown that cure of prophage was achieved. This can be considered one additional biological effect of 8MOP-NUV association. Following 8MOP-NUV treatment, variants which were tetracycline sensitive were obtained. The observed frequencies were higher than those achieved spontaneously or after ethidium bromide and high temperature treatments. Gel agarose electrophoresis were carried out from the original strain and non-resistant variants lysates. The results have shown that loss of resistance correspond to a loss of a band on agarose gel which must correspond to the plasmid carrying the tetracycline resistance gene. In this way 8MOP-NUV association can be considered as another agent able to eliminate plasmids from S. aureus

Synthesis, Molecular Properties Prediction, and Anti-staphylococcal Activity of N-Acylhydrazones and New 1,3,4-Oxadiazole Derivatives

Five new 1-(2-(5-nitrofuran-2-yl)-5-(aryl)-1,3,4-oxadiazol-3-(2H)-yl) ethanone compounds 5a–e were synthesized by cyclization of N-acylhydrazones 4a–e with acetic anhydride under reflux conditions. Their structures were fully characterized by IR, 1H-NMR, and 13C-NMR. Furthermore, evaluations of the antibacterial activity of the 1,3,4-oxadiazoles 5a–e and N-acylhydrazones 4a–e showed strong activity against several strains of Staphylococcus aureus, with MICs between 4 μg/mL to 32 μg/mL. In silico studies of the parameters of Lipinski’s Rule of Five, as well as the topological polar surface area (TPSA), absorption percentage (% ABS), drug likeness and drug score indicate that these compounds, especially 4a and 5d, have potential to be new drug candidates

Update on the Cardiopulmonary Resuscitation and Emergency Cardiovascular Care Guideline of the Brazilian Society of Cardiology-2019

sem informação113344966

Seminário de Dissertação (2024)

Página da disciplina de Seminário de Dissertação (MPPP, UFPE, 2022) Lista de participantes == https://docs.google.com/spreadsheets/d/1mrULe1y04yPxHUBaF50jhaM1OY8QYJ3zva4N4yvm198/edit#gid=

NEOTROPICAL CARNIVORES: a data set on carnivore distribution in the Neotropics

Mammalian carnivores are considered a key group in maintaining ecological health and can indicate potential ecological integrity in landscapes where they occur. Carnivores also hold high conservation value and their habitat requirements can guide management and conservation plans. The order Carnivora has 84 species from 8 families in the Neotropical region: Canidae; Felidae; Mephitidae; Mustelidae; Otariidae; Phocidae; Procyonidae; and Ursidae. Herein, we include published and unpublished data on native terrestrial Neotropical carnivores (Canidae; Felidae; Mephitidae; Mustelidae; Procyonidae; and Ursidae). NEOTROPICAL CARNIVORES is a publicly available data set that includes 99,605 data entries from 35,511 unique georeferenced coordinates. Detection/non-detection and quantitative data were obtained from 1818 to 2018 by researchers, governmental agencies, non-governmental organizations, and private consultants. Data were collected using several methods including camera trapping, museum collections, roadkill, line transect, and opportunistic records. Literature (peer-reviewed and grey literature) from Portuguese, Spanish and English were incorporated in this compilation. Most of the data set consists of detection data entries (n = 79,343; 79.7%) but also includes non-detection data (n = 20,262; 20.3%). Of those, 43.3% also include count data (n = 43,151). The information available in NEOTROPICAL CARNIVORES will contribute to macroecological, ecological, and conservation questions in multiple spatio-temporal perspectives. As carnivores play key roles in trophic interactions, a better understanding of their distribution and habitat requirements are essential to establish conservation management plans and safeguard the future ecological health of Neotropical ecosystems. Our data paper, combined with other large-scale data sets, has great potential to clarify species distribution and related ecological processes within the Neotropics. There are no copyright restrictions and no restriction for using data from this data paper, as long as the data paper is cited as the source of the information used. We also request that users inform us of how they intend to use the data

Reduction of cardiac imaging tests during the COVID-19 pandemic: The case of Italy. Findings from the IAEA Non-invasive Cardiology Protocol Survey on COVID-19 (INCAPS COVID)

Background: In early 2020, COVID-19 massively hit Italy, earlier and harder than any other European country. This caused a series of strict containment measures, aimed at blocking the spread of the pandemic. Healthcare delivery was also affected when resources were diverted towards care of COVID-19 patients, including intensive care wards. Aim of the study: The aim is assessing the impact of COVID-19 on cardiac imaging in Italy, compare to the Rest of Europe (RoE) and the World (RoW). Methods: A global survey was conducted in May–June 2020 worldwide, through a questionnaire distributed online. The survey covered three periods: March and April 2020, and March 2019. Data from 52 Italian centres, a subset of the 909 participating centres from 108 countries, were analyzed. Results: In Italy, volumes decreased by 67% in March 2020, compared to March 2019, as opposed to a significantly lower decrease (p &lt; 0.001) in RoE and RoW (41% and 40%, respectively). A further decrease from March 2020 to April 2020 summed up to 76% for the North, 77% for the Centre and 86% for the South. When compared to the RoE and RoW, this further decrease from March 2020 to April 2020 in Italy was significantly less (p = 0.005), most likely reflecting the earlier effects of the containment measures in Italy, taken earlier than anywhere else in the West. Conclusions: The COVID-19 pandemic massively hit Italy and caused a disruption of healthcare services, including cardiac imaging studies. This raises concern about the medium- and long-term consequences for the high number of patients who were denied timely diagnoses and the subsequent lifesaving therapies and procedures

International Impact of COVID-19 on the Diagnosis of Heart Disease

Background: The coronavirus disease 2019 (COVID-19) pandemic has adversely affected diagnosis and treatment of noncommunicable diseases. Its effects on delivery of diagnostic care for cardiovascular disease, which remains the leading cause of death worldwide, have not been quantified. Objectives: The study sought to assess COVID-19's impact on global cardiovascular diagnostic procedural volumes and safety practices. Methods: The International Atomic Energy Agency conducted a worldwide survey assessing alterations in cardiovascular procedure volumes and safety practices resulting from COVID-19. Noninvasive and invasive cardiac testing volumes were obtained from participating sites for March and April 2020 and compared with those from March 2019. Availability of personal protective equipment and pandemic-related testing practice changes were ascertained. Results: Surveys were submitted from 909 inpatient and outpatient centers performing cardiac diagnostic procedures, in 108 countries. Procedure volumes decreased 42% from March 2019 to March 2020, and 64% from March 2019 to April 2020. Transthoracic echocardiography decreased by 59%, transesophageal echocardiography 76%, and stress tests 78%, which varied between stress modalities. Coronary angiography (invasive or computed tomography) decreased 55% (p &lt; 0.001 for each procedure). In multivariable regression, significantly greater reduction in procedures occurred for centers in countries with lower gross domestic product. Location in a low-income and lower–middle-income country was associated with an additional 22% reduction in cardiac procedures and less availability of personal protective equipment and telehealth. Conclusions: COVID-19 was associated with a significant and abrupt reduction in cardiovascular diagnostic testing across the globe, especially affecting the world's economically challenged. Further study of cardiovascular outcomes and COVID-19–related changes in care delivery is warranted