A factorial design for optimization of the analytical variables on the development of a genoassay for the transgenic soybean detection

At the laboratory, analytical method optimizations are performed to achieve the maximum sensitivity and selectivity. Routinely, this procedure is carried out by optimizing one-factor-at-a-time approach until there is no further improvement, where each experimental parameter is optimized separately and independently of the other factors.N/

REPORT ON THE 2020 ICCAT WORKSHOP ON SMALL TUNAS BIOLOGY STUDIES FOR GROWTH AND REPRODUCTION.

SUMMARY This report describes the 2020 ICCAT workshop on small tunas biology studies for growth and reproduction, hosted by the Instituto Español de Oceanografía, Málaga, Spain. The major objectives of the workshop were: 1) starting the creation of ageing and reproduction reference sets and, 2) providing more training for the ongoing sample collection and processing to the teams involved in these studies. As approved by the SCRS in 2017, the Small Tuna Species Group intersessional meeting decided to prioritize the collection of biological samples aiming at growth, maturity and stock structure studies on three species: little tunny (Euthynnus alletteratus), Atlantic Bonito (Sarda sarda) and wahoo (Acanthocybium solandri), based on their economic importance and the lack of knowledge on their biology. This work will contribute to the next major advance in the assessment of these three species. RÉSUMÉ Le présent rapport décrit l'atelier de l’ICCAT tenu en 2020 sur les études de la biologie des thonidés mineurs pour la croissance et la reproduction, organisé par l'Instituto Español de Oceanografía, à Malaga, en Espagne. Les principaux objectifs de l'atelier étaient les suivants : 1) commencer à créer des ensembles de référence sur la détermination de l’âge et la reproduction et 2) fournir une formation plus poussée sur la collecte et le traitement des échantillons aux équipes participant à ces études. Tel qu’approuvé par le SCRS en 2017, lors de la réunion intersessions du Groupe d'espèces sur les thonidés mineurs, il a été décidé de donner la priorité à la collecte d'échantillons biologiques visant à étudier la croissance, la maturité et la structure des stocks de trois espèces : la thonine commune (Euthynnus alletteratus), la bonite à dos rayé (Sarda) et le thazard-bâtard (Acanthocybium solandri), sur la base de leur importance économique et des connaissances lacunaires sur leur biologie. Ces travaux contribueront à la prochaine grande avancée dans l'évaluation de ces trois espèces. RESUMEN Este informe describe el taller de ICCAT de 2020 sobre estudios de biología de pequeños túnidos para crecimiento y reproducción, acogido por el Instituto Español de Oceanografía en Málaga, España. Los principales objetivos del taller eran: 1) empezar la creación de conjuntos de referencia de determinación de la edad y reproducción y 2) facilitar más formación a los equipos involucrados en estos estudios para la recopilación de muestras y procesamiento en curso. Como aprobó el SCRS en 2017, en la Reunión intersesiones del Grupo de especies de pequeños túnidos se decidió priorizar la recopilación de muestras biológicas con miras a estudios de crecimiento, madurez y estructura del stock de tres especies: bacoreta (Euthynnus alletteratus), bonito (Sarda sarda) y peto (Acanthocybium solandri), basándose en su importancia económica y la falta de conocimientos sobre su biología. Este trabajo contribuirá a avanzar en la próxima evaluación de estas tres especies.Versión del edito

Final report of the short-term contract for ICCAT SMYTP for the biological samples collection for growth, maturity and genetics studies – Year #2

This document is the final report of the second year of the short-term contract of the Small Tuna Year Program by ICCAT. In 2018, the Small Tuna Species Group decided to prioritize Little tunny (LTA) (Euthynnus alletteratus) Atlantic bonito (BON) (Sarda sarda) and Wahoo (WAH) (Acanthocybium solandri), based on their economic importance and the deficiency of knowledge of their biology. The objectives of the contract for three species were: i) Collect biological samples for estimating growth parameters, assessing the maturity and stock structure analysis (populations genetics), and ii) Conclude the analysis of the stock structure for at least one of the three species and provide preliminary results for the remaining. The obtained samples for growth, maturity and stock structure analysis was almost completed for Little tunny and Atlantic bonito, whereas for Wahoo the samples are scarce. The analysis of stock structure for Little tunny and Atlantic bonito revealed that the observed dif

Report on the 2020 ICCAT workshop on small tunas biology studies for growth and reproduction

This report describes the 2020 ICCAT workshop on small tunas biology studies for growth and reproduction, hosted by the Instituto Español de Oceanografía, Málaga, Spain. The major objectives of the workshop were: 1) starting the creation of ageing and reproduction reference sets and, 2) providing more training for the ongoing sample collection and processing to the teams involved in these studies. As approved by the SCRS in 2017, the Small Tuna Species Group intersessional meeting decided to prioritize the collection of biological samples aiming at growth, maturity and stock structure studies on three species: little tunny (Euthynnus alletteratus), Atlantic Bonito (Sarda sarda) and wahoo (Acanthocybium solandri), based on their economic importance and the lack of knowledge on their biology. This work will contribute to the next major advance in the assessment of these three species

Pharmacological inhibition of lysine-specific demethylase 1 (LSD1) induces global transcriptional deregulation and ultrastructural alterations that impair viability in Schistosoma mansoni

Treatment and control of schistosomiasis still rely on only one effective drug, praziquantel (PZQ) and, due to mass treatment, the increasing risk of selecting for schistosome strains that are resistant to PZQ has alerted investigators to the urgent need to develop novel therapeutic strategies. The histone-modifying enzymes (HMEs) represent promising targets for the development of epigenetic drugs against Schistosoma mansoni. In the present study, we targeted the S. mansoni lysine-specific demethylase 1 (SmLSD1), a transcriptional corepressor, using a novel and selective synthetic inhibitor, MC3935, which was used to treat schistosomula and adult worms in vitro. By using cell viability assays and optical and electron microscopy, we showed that treatment with MC3935 affected parasite motility, egg-laying, tegument, and cellular organelle structures, culminating in the death of schistosomula and adult worms. In silico molecular modeling and docking analysis suggested that MC3935 binds to the catalytic pocket of SmLSD1. Western blot analysis revealed that MC3935 inhibited SmLSD1 demethylation activity of H3K4me1/2. Knockdown of SmLSD1 by RNAi recapitulated MC3935 phenotypes in adult worms. RNA-Seq analysis of MC3935-treated parasites revealed significant differences in gene expression related to critical biological processes. Collectively, our findings show that SmLSD1 is a promising drug target for the treatment of schistosomiasis and strongly support the further development and in vivo testing of selective schistosome LSD1 inhibitors

Quaternized cashew gum: An anti-staphylococcal and biocompatible cationic polymer for biotechnological applications

Chemical modifications to cashew gum (CG) structure have been previously reported to obtain new physicochemical characteristics, however until now there were no reports of modifications by introduction of new functional groups to add cationic character. This study presents a quaternization route for CG using a quaternary ammonium reagent. The chemical features of the quaternized cashew gum derivatives (QCG) were analyzed by: FTIR, elemental analysis, degree of substitution, Zeta potential, 1H NMR and 1H-13C correlation (HSQC). QCG were evaluated for their anti-staphylococcal activity by determining minimum inhibitory and bactericidal concentrations against pathogenic Staphylococcus spp. and by imaging using atomic force microscopy. Moreover, the mammalian cell biocompatibility were also assessed through hemolytic and cell toxicity assays. QCG presented promising antimicrobial activity against methicillin-resistant S. aureus and biocompatibility on tested cells. These results show that QCG could be a promising tool in the development of biomaterials with an anti-septic action.info:eu-repo/semantics/publishedVersio