Efecto del contenido de BaSO y DEAEA 4 sobre las propiedades reológicas de cementos óseos para vertebroplastia.

En este trabajo se estudió el efecto del contenido de sulfato de bario (BaSO ) y del acrilato del 2-dietilamino etilo 4 (DEAEA) sobre el comportamiento reológico (inyectabilidad y viscosidad) de cementos óseos acrílicos, empleando para esto un diseño experimental con gráficos de superficies de respuesta y de contorno. Los resultados mostraron que un aumento en el contenido de BaSO produce una disminución de la viscosidad y un aumento de la 4 inyectabilidad del cemento óseo en estado fresco; en contraste, el DEAEA solo parece tener un ligero efecto sobre la inyectabilidad del mismo. La formulación con 4 % de DEAEA y 30 % de BaSO resultó tener las mejores propiedades 4 reológicas para su posible aplicación en vertebroplastia percutánea (VPP), por lo que esta formulación fue caracterizada fisicoquímica y mecánicamente; los resultados obtenidos en estos análisis fueron comparados con el cemento óseo comercial Zimmer Dough

Electrospun polycaprolactone/chitosan scaffolds for nerve tissue engineering: physicochemical characterization and Schwann cell biocompatibility

[EN] Electrospun polycaprolactone (PCL)/chitosan (CH) blend scaffolds with different CH weight ratios were prepared to study the effect of scaffold composition on its physicochemical and biological properties. Scanning electron microscopy showed bead-free homogeneous randomly arranged nanofibers whose average diameter decreased from 240 to 110 nm with increasing CH content. The infrared spectra of the PCL/CH blends were very similar to the neat PCL scaffold. Energy-dispersive x-ray spectroscopy analysis confirmed the presence of carbon, oxygen and nitrogen in the scaffolds, although fluorine-from chemicals used as solvent-was also detected. The water contact angle decreased from 113 degrees (for PCL) to 52 degrees with increasing chitosan content. The biocompatibility was evaluated using fibroblasts and Schwann cell (SC) cultures. Cytotoxicity assays using fibroblasts demonstrated that electrospun scaffolds could be considered as non-cytotoxic material. Biocompatibility tests also revealed that the SCs adhered to scaffolds with different CH content, although the formulation containing CH at 5 wt% exhibited the highest proliferation on days 1 and 3. A better cell distribution was observed in the CH/PCL blends than in the neat PCL or CH scaffolds, where the cells were clustered. Immunochemistry analysis confirmed that SCs expressed the specific p75 cell marker on the scaffolds, suggesting that PCL/CH scaffolds would be good candidates for peripheral nerve tissue engineering.This work was supported by CONACYT (Mexico) grant CB 2011-169698-Y. Ena Bolaina-Lorenzo acknowledges CONACYT for her scholarship (236153) and for her internship support (290842) through the 'BECAS MIXTAS' program. CMR and MMP acknowledge support of the Spanish Ministry through project MAT2015-66666-C3-1-R. Assistance and advice received from the Electron Microscopy Service at the Universitat Politecnica de Valencia is also acknowledged. 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