An Improved Method for Obtaining Small Pressed Powder Pellets for the Analysis by XRF

A rapid, very low cost of instrumentation and simple approach to specimen preparation for the analysis by X-ray fluorescence spectrometry is presented. This technique requires a few mg of powdered samples, which are compressed to produce pellets. This procedure is employed for determining major, minor and trace elements in muscovites and K-feldspars. Factors affecting measured intensities, such as particle size and distribution, surface texture, pelletizing pressure, etc, are considered. A description of the new sample preparation device, including technical characteristics, is also given. It is demonstrated that suitable specimen presentation to the X-ray beam is obtained by using our procedure.Fil: D`angelo, José Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Strasser, Edgardo. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Marchevsky, Eduardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Perino, Ernesto. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentin

Chemotaxonomic comparison of the seed ferns Odontopteris cantabrica and Odontopteris schlotheimii, Middle Pennsylvanian Sydney Coalfield, Canada

We present the first comparative spectrochemical data in the study history of Odontopteris schlotheimii Brongniart, 1828–1831 and Odontopteris cantabrica Wagner, 1969. These fossils co-occur in the shaley roof of the Lloyd Cove seam (Ro% = 0.65; Sydney Coalfield, Canada). Fourier transform-infrared spectra show that the sampled pinnules of both species are preserved as fossilized-cuticles. They are prominently aliphatic with distinct methylene and methyl peaks (at 2922 cm–1 and 2852 cm–1); with relatively high contents of carbonyl groups (peaks at 1700 cm–1) that indicate a high degree of molecular cross linking; and with smaller amounts of aromatics (peaks at 820 cm–1 and 750 cm–1) with a low condensation degree of benzene rings. No differences among respective pinnules are inferred from the 3D chemometric model and associate significance tests by the analysis of variance (α = 0.05). At the same time, this method provides new, chemical parameters that, in conjunction with morphological features, could be used to efficiently distinguish species.Fil: D`angelo, José Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Cape Breton University; CanadáFil: Zodrow, Erwin L.. Cape Breton University; Canad

Sterile foliage of fertile Sydneia manleyi and synangial chemistry (eusporangiate fern, Late Asturian, Canada): A new subfamily Sydneideae

Typification of Sydneia manleyi Psenicka et al. 2003 is based entirely on fertile foliage. Of late,attached sterile-fertile segments have been found which are illustrated and described, together with cuticular preparations. A new subfamily, Sydneideae subfam. nov. is erected for the monotypytic species. The synangial chemistry is compared with marattialean  synangia/sporangia from the Czech Republic and from Canada.Fil: Psenicka, Josef. West Bohemian Museum in Pilsen. Palaeontological Department; República ChecaFil: Zodrow, Erwin L.. Cape Breton Universit. Palaeobiology Laboratory; CanadáFil: D` Angelo, José Alejandro. Cape Breton Universit. Palaeobiology Laboratory; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas. Científico Tecnológico Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; Argentin

The Neuropteris ovata frond and its cyclopteroids: Micromorphology-spectrochemistry-fractal taxonomy. Propositions for restructuring and taxonomy (Pennsylvanian, Canada)

The primary study material consists of the 65-cm frond Neuropteris ovata (Hoffmann) var. simonii, associate petiole with organically attached cyclopteroid leaflets and trunk. Ancillary N. ovata material is used, all from the Asturian-Cantabrian strata of the Sydney Coalfield, Canada. This material is appropriate for the objective of the study to present an ovata-frond Aufbau in terms of micromorphology coupled with chemistry using an holistic sampling design (co-ordinating epidermal microscopy with chemistry per sample location). Chemical analyses were obtained by the reliable FTIR technique (Fourier transform infrared spectroscopy) and interpreted via chemometrics by methods of principal component analysis. Fractal geometry is introduced as a promising taxonomic parameter. The main conclusions include that the N. ovata plant can be characterized by three distinct epidermal structures: (1) rectangular elongate (trunk and petiole), (2) elongate (rachides of three orders), and (3) undulate (pinnule), which to a certain extent agrees with the chemistry of the frond Aufbau. The frond was also complex - architecturally, histologically, physiologically, chemically and autecologically. It was photosynthetic, had secretory organs (implying a characteristic plant aroma), supporting rodlets, divers epicuticular features, and a distinct chemical composition/structure. The frond was likely 4 m long with complex-pinnate cyclopteroid leaflets, fimbriate or entire-margined, below and above the main bifurcation, which begs the question of cyclopteroid definition and function. The overall results will be beneficial to taxonomy/systematics, and guide pteridospermous reconstruction into a new paradigm.Fil: Zodrow, E. L.. Cape Breton University; CanadáFil: D`angelo, José Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; ArgentinaFil: Cleal, C.. National Museum Wales; Reino Unid

3D chemometric model simulating the Acitheca polymorpha frond: implications for reconstructing carboniferous ferns (Marattiales, Canada)

Reported are results of an initial approximate imitation of a Carboniferous fern frond, i.e., marattialean Acitheca polymorpha (Schimper), Middle Pennsylvanian Sydney Coalfield, Canada. The simulation experiment is based on the analysis of 14 infrared spectra obtained by means of Fourier Transform Infrared spectroscopy from four detached fragments of sterile polymorphic penultimate-pinna compressions. The calculated relative, semi-quantitative, chemical data from the infrared spectra are the input for principal component analysis deriving a 3D (three-dimensional) chemometric model. To interpret it, the four specimens are placed in hypothetical-frond positions simulating a tripinnate frond, based on diminishing penultimate-rachial widths from 1-mm (distal) to 10-mm (proximal). Hypothetical conclusions include position-dependent chemistries, specifically that of opposing trends of aromaticity vs. aliphaticity in pinnules-rachises. This, in turn, would suggest potential for (i) fern-frond reconstruction, and (ii) for determination of a most likely frond position of fragmentary specimens by “chemical classification”; the predictive aspect. However, further experimental refinement is necessary particularly based on larger frond segments to confirm or disconfirm the overall hypothetical results.Fil: D`angelo, José Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; Argentina. Cape Breton University; CanadáFil: Zodrow, Erwin L.. Cape Breton University; CanadáFil: Psenicka, Josef. West Bohemian Museum ; República Chec

Non-Parallel Articulatory-to-Acoustic Conversion Using Multiview-based Time Warping

This work was supported in part by the Spanish State Research Agency (SRA) grant number PID2019-108040RB-C22/SRA/10.13039/501100011033, and the FEDER/Junta de AndalucíaConsejería de Transformación Económica, Industria, Conocimiento y Universidades project no. B-SEJ-570-UGR20.In this paper, we propose a novel algorithm called multiview temporal alignment by dependence maximisation in the latent space (TRANSIENCE) for the alignment of time series consisting of sequences of feature vectors with different length and dimensionality of the feature vectors. The proposed algorithm, which is based on the theory of multiview learning, can be seen as an extension of the well-known dynamic time warping (DTW) algorithm but, as mentioned, it allows the sequences to have different dimensionalities. Our algorithm attempts to find an optimal temporal alignment between pairs of nonaligned sequences by first projecting their feature vectors into a common latent space where both views are maximally similar. To do this, powerful, nonlinear deep neural network (DNN) models are employed. Then, the resulting sequences of embedding vectors are aligned using DTW. Finally, the alignment paths obtained in the previous step are applied to the original sequences to align them. In the paper, we explore several variants of the algorithm that mainly differ in the way the DNNs are trained. We evaluated the proposed algorithm on a articulatory-to-acoustic (A2A) synthesis task involving the generation of audible speech from motion data captured from the lips and tongue of healthy speakers using a technique known as permanent magnet articulography (PMA). In this task, our algorithm is applied during the training stage to align pairs of nonaligned speech and PMA recordings that are later used to train DNNs able to synthesis speech from PMA data. Our results show the quality of speech generated in the nonaligned scenario is comparable to that obtained in the parallel scenario.Spanish State Research Agency (SRA) PID2019-108040RB-C22/SRA/10.13039/501100011033FEDER/Junta de AndalucíaConsejería de Transformación Económica, Industria, Conocimiento y Universidades project no. B-SEJ-570-UGR20

Multi-view Temporal Alignment for Non-parallel Articulatory-to-Acoustic Speech Synthesis

Articulatory-to-acoustic (A2A) synthesis refers to the generation of audible speech from captured movement of the speech articulators. This technique has numerous applications, such as restoring oral communication to people who cannot longer speak due to illness or injury. Most successful techniquesso far adopt a supervised learning framework, in which timesynchronousarticulatory-and-speech recordings are used to train a supervised machine learning algorithm that can be used later to map articulator movements to speech. This, however, prevents the application of A2A techniques in cases where parallel data is unavailable, e.g., a person has already lost her/his voice and only articulatory data can be captured. In this work, we propose a solution to this problem based on the theory of multi-view learning. The proposed algorithm attempts to find an optimal temporal alignment between pairs of nonaligned articulatory-and-acoustic sequences with the same phonetic content by projecting them into a common latent space where both views are maximally correlated and then applying dynamic time warping. Several variants of this idea are discussed and explored. We show that the quality of speech generated in the non-aligned scenario is comparable to that obtained in the parallel scenario.This work was funded by the Spanish State Research Agency (SRA) under the grant PID2019-108040RBC22/ SRA/10.13039/501100011033. Jose A. Gonzalez-Lopez holds a Juan de la Cierva-Incorporation Fellowship from the Spanish Ministry of Science, Innovation and Universities (IJCI-2017-32926)

Gene Expression Response to Sea Lice in Atlantic Salmon Skin: RNA Sequencing Comparison Between Resistant and Susceptible Animals

Sea lice are parasitic copepods that cause large economic losses to salmon aquaculture worldwide. Frequent chemotherapeutic treatments are typically required to control this parasite, and alternative measures such as breeding for improved host resistance are desirable. Insight into the host–parasite interaction and mechanisms of host resistance can lead to improvements in selective breeding, and potentially novel treatment targets. In this study, RNA sequencing was used to study the skin transcriptome of Atlantic salmon (Salmo salar) parasitized with sea lice (Caligus rogercresseyi). The overall aims were to compare the transcriptomic profile of skin at louse attachment sites and “healthy” skin, and to assess differences in gene expression response between animals with varying levels of resistance to the parasite. Atlantic salmon pre-smolts were challenged with C. rogercresseyi, growth and lice count measurements were taken for each fish. 21 animals were selected and RNA-Seq was performed on skin from a louse attachment site, and skin distal to attachment sites for each animal. These animals were classified into family-balanced groups according to the traits of resistance (high vs. low lice count), and growth during infestation. Overall comparison of skin from louse attachment sites vs. healthy skin showed that 4,355 genes were differentially expressed, indicating local up-regulation of several immune pathways and activation of tissue repair mechanisms. Comparison between resistant and susceptible animals highlighted expression differences in several immune response and pattern recognition genes, and also myogenic and iron availability factors. Components of the pathways involved in differential response to sea lice may be targets for studies aimed at improved or novel treatment strategies, or to prioritize candidate functional polymorphisms to enhance genomic selection for host resistance in commercial salmon breeding programs

Low genetic diversity associated with low prevalence of Anaplasma marginale in water buffaloes in Marajó Island, Brazil

The rickettsia Anaplasma marginale is the etiologic agent of bovine anaplasmosis, an important tick-borne disease affecting cattle in tropical and subtropical regions of the world. In endemic regions, the genetic diversity of this pathogen is usually related to the high prevalence of the disease in cattle. The major surface protein 1 alpha (MSP1a) has been used as a marker to characterize the genetic diversity and for geographical identification of A. marginale strains. The present study reports the characterization of A. marginale MSP1a diversity in water buffaloes. Blood samples were collected from 200 water buffaloes on Marajó Island, Brazil where the largest buffalo herd is located in the Western hemisphere. Fifteen buffaloes (7.5%) were positive for A. marginale msp1α by PCR. Four different strains of A. marginale with MSP1a tandem repeat structures (4-63-27), (162-63-27), (78-24-24-25-31) and (τ-10-10-15) were found, being (4-63-27) the most common. MSP1a tandem repeats composition in buffalos and phylogenetic analysis using msp1α gene showed that the A. marginale strains identified in buffaloes are closely related to A. marginale strains from cattle. The results demonstrated low genetic diversity of A. marginale associated with low bacterial prevalence in buffaloes and suggested that buffaloes may be reservoirs of this pathogen for cattle living in the same area. The results also suggested that mechanical transmission and not biological transmission by ticks might be playing the major role for pathogen circulation among water buffaloes in Marajó Island, Brazil.We thank the Coordination for the Improvement of Higher Education Personnel (CAPES) foundation and the National Council for Technological and Scientific Development (CNPq) for their financial support.Peer Reviewe