Functional analysis of novel genes differentially expressed genes in heart/hemangioblast precursor cells (H/HPC)

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Cerberus is a feedback inhibitor of Nodal asymmetric signaling in the chick embryo

The TGF-beta-related molecule Nodal plays an essential and conserved role in left-right patterning of the vertebrate embryo. Previous reports have shown that the zebrafish and mouse Cerberus-related proteins Charon and Cerberus-like-2 (Cerl-2), respectively, act in the node region to prevent the Nodal signal from crossing to the right side, whereas chick Cerberus (cCer) has an unclear function in the left-side mesoderm. In this study, we investigate the transcriptional regulation and function of cCer in left-right development. By analyzing the enhancer activity of cCer 5' genomic sequences in electroporated chick embryos, we identified a cCer left-side enhancer that contains two FoxH1 and one SMAD binding site. We show that these Nodal-responsive elements are necessary and sufficient for the activation of transcription in the left-side mesoderm. In transgenic mouse embryos, cCer regulatory sequences behave as in chick embryos, suggesting that the cis-regulatory sequences of Cerberus-related genes have diverged during vertebrate evolution. Moreover, our findings from cCer overexpression and knockdown experiments indicate that cCer is a negative-feedback regulator of Nodal asymmetric signaling. We propose that cCer and mouse Cerl-2 have evolved distinct regulatory mechanisms but retained a conserved function in left-right development, which is to restrict Nodal activity to the left side of the embryo.info:eu-repo/semantics/publishedVersio

The BRIKEN Project: extensive measurements of ß-delayed neutron emitters for the astrophysical r process

An ambitious program to measure decay properties, primarily ß-delayed neutron emission probabilities and half-lives, for a significant number of nuclei near or on the path of the rapid neutron capture process, has been launched at the RIKEN Nishina Center. We give here an overview of the status of the project.Postprint (published version

Multiple manipulators path planning using double A∗

Streamlining automated processes is currently undertaken by developing optimization methods and algorithms for robotic manipulators. This paper aims to present a new approach to improve streamlining of automatic processes. This new approach allows for multiple robotic manipulators commonly found in the industrial environment to handle different scenarios, thus providing a high-flexibility solution to automated processes. Design/methodology/approach - The developed system is based on a spatial discretization methodology capable of describing the surrounding environment of the robot, followed by a novel path-planning algorithm. Gazebo was the simulation engine chosen, and the robotic manipulator used was the Universal Robot 5 (UR5). The proposed system was tested using the premises of two robotic challenges: EuRoC and Amazon Picking Challenge. Findings - The developed system was able to identify and describe the influence of each joint in the Cartesian space, and it was possible to control multiple robotic manipulators safely regardless of any obstacles in a given scene. Practical implications - This new system was tested in both real and simulated environments, and data collected showed that this new system performed well in real-life scenarios, such as EuRoC and Amazon Picking Challenge. Originality/value - The new proposed approach can be valuable in the robotics field with applications in various industrial scenarios, as it provides a flexible solution for multiple robotic manipulator path and motion planning.The research leading to these results has received funding from the project "NORTE-01-0145-FEDER-000020" which is nanced by the North Portugal Re- gional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, and through the European Regional Development Fund (ERDF).info:eu-repo/semantics/publishedVersio

Exchange Rates and Fiscal Adjustments; Evidence from the OECD and Implications for the EMU

This paper investigates whether monetary and exchange rate policies are important for the success of major fiscal adjustments. We assess their role controlling for other determinants of success identified in the literature, including the size and composition of the deficit cut, the level of public debt and the rate of economic growth. We find that successful adjustments are preceded by exchange rate depreciations. Empirically, a depreciation of the nominal effective exchange of one standard deviation of the sample mean in the two years before an adjustment increases the probability of success by 2 percentage points. The size and composition of the deficit cut are also important determinants of success: an increase of one standard deviation of the sample mean raises the probability of success by 3 and 4 percentage points, respectively. One implication of our results is that it may be more difficult to attain persistent fiscal adjustments within the Economic and Monetary Union of Europe, since the adoption of a single currency rules out the use of exchange rate policy among member countries

Exploiting Template Metaprogramming to customize an object-oriented operating system

Nowadays, the growing complexity of embedded systems demands for configurability, variability and reuse. Conditional compilation and object-orientation are two of the most applied approaches in the management of system variability. While the former increases the code management complexity, the latter leverages the needed modularity and adaptability to simplify the development of reusable and customizable software at the expense of performance and memory penalty. This paper shows how C++ TMP (Template Metaprogramming) can be applied to manage the variability of an object-oriented operating system and at the same time get ride out of the performance and memory footprint overhead. In doing so, it will be statically generated only the desired functionalities, thus ensuring that code is optimized and adjusted to application requirements and hardware resources.Fundação para a Ciência e a Tecnologia (FCT

Identification of differentially expressed genes in the heart precursor cells of the chick embryo

Genetic evidence has implicated several genes as being critical for heart development. However, the inducers of these genes as well as their targets and pathways they are involved with, remain largely unknown. Previous studies in the avian embryo showed that at HH4 Cerberus (cCer) transcripts are detected in the anterior endomesoderm including the heart precursor cells and later in the left lateral plate mesoderm. We have identified a promoter element of chick cCer able to drive EGFP expression in a population of cells that consistently exit from the anterior primitive streak region, from as early as stage HH3+, and that later will populate the heart. Using this promoter element as a tool allowed us to identify novel genes previously not known to potentially play a role in heart development. In order to identify and study genes expressed and involved in the correct development and differentiation of the vertebrate heart precursor cell (HPC) lineages, a differential screening using Affymetrix GeneChip system technologies was performed. Remarkably, this screening led to the identification of more than 700 transcripts differentially expressed in the heart forming regions (HFR). Bioinformatic tools allowed us to filter the large amount of data generated from this approach and to select a few transcripts for in vivo validation. Whole-mount in situ hybridization and sectioning of selected genes showed heart and vascular expression patterns for these transcripts during early chick development. We have developed an effective strategy to specifically identify genes that are differentially expressed in the HPC lineages. Within this set we have identified several genes that are expressed in the heart, blood and vascular lineages, which are likely to play a role in their development. These genes are potential candidates for future functional studies on early embryonic patterning.Fundação para a Ciência e a Tecnologia Ph.D., PD and BI fellowships; FCT research grants; IGC/FCG; IBB/CBME

Damaging flood risk in the Portuguese municipalities

Modeling and understanding the impact of climate change on flooding processes in Mediterranean climate areas, namely in southern Europe, is a complex endeavor, which must also consider exposure and vulnerability patterns. Assuming that vulnerability plays a relevant role in explaining the degree of loss due to natural hazards, the present research compares a flood-susceptibility index with a social-vulnerability index and a historical record of flood losses, both aggregated at the municipal level. The purpose of this research is to define municipal flood risk profiles that would rank the 278 municipalities and contribute to the strategic allocation of resources and flood risk management [...]info:eu-repo/semantics/publishedVersio