Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge

Microbial communities and their associated metabolic activity in marine sediments have a profound impact on global biogeochemical cycles. Their composition and structure are attributed to geochemical and physical factors, but finding direct correlations has remained a challenge. Here we show a significant statistical relationship between variation in geochemical composition and prokaryotic community structure within deep-sea sediments. We obtained comprehensive geochemical data from two gravity cores near the hydrothermal vent field Loki’s Castle at the Arctic Mid-Ocean Ridge, in the Norwegian- Greenland Sea. Geochemical properties in the rift valley sediments exhibited strong centimeter-scale stratigraphic variability. Microbial populations were profiled by pyrosequencing from 15 sediment horizons (59,364 16S rRNA gene tags), quantitatively assessed by qPCR, and phylogenetically analyzed. Although the same taxa were generally present in all samples, their relative abundances varied substantially among horizons and fluctuated between Bacteria- and Archaea-dominated communities. By independently summarizing covariance structures of the relative abundance data and geochemical data, using principal components analysis, we found a significant correlation between changes in geochemical composition and changes in community structure. Differences in organic carbon and mineralogy shaped the relative abundance of microbial taxa. We used correlations to build hypotheses about energy metabolisms, particularly of the Deep Sea Archaeal Group, specific Deltaproteobacteria, and sediment lineages of potentially anaerobic Marine Group I Archaea. We demonstrate that total prokaryotic community structure can be directly correlated to geochemistry within these sediments, thus enhancing our understanding of biogeochemical cycling and our ability to predict metabolisms of uncultured microbes in deep-sea sediments

Measurements of binary stars with coherent integration of NPOI data

In this paper we use coherently integrated visibilities (see separate paper in these proceedings, Jorgensen et al. 2008) to measure the properties of binary stars. We use only the phase of the complex visibility and not the amplitude. The reason for this is that amplitudes suffer from the calibration effect (the same for coherent and incoherent averages) and thus effectively provide lower accuracy measurements. We demonstrate that the baseline phase alone can be used to measure the separation, orientation and brightness ratio of a binary star, as a function of wavelength.Comment: 2008 SPIE Astronomical Telescopes and Instrumentatio

Cryptosporidium, Enterocytozoon, and Cyclospora Infections in Pediatric and Adult Patients with Diarrhea in Tanzania.

Cryptosporidiosis, microsporidiosis, and cyclosporiasis were studied in four groups of Tanzanian inpatients: adults with AIDS-associated diarrhea, children with chronic diarrhea (of whom 23 of 59 were positive [+] for human immunodeficiency virus [HIV]), children with acute diarrhea (of whom 15 of 55 were HIV+), and HIV control children without diarrhea. Cryptosporidium was identified in specimens from 6/86 adults, 5/59 children with chronic diarrhea (3/5, HIV+), 7/55 children with acute diarrhea (0/7, HIV+), and 0/20 control children. Among children with acute diarrhea, 7/7 with cryptosporidiosis were malnourished, compared with 10/48 without cryptosporidiosis (P < .01). Enterocytozoon was identified in specimens from 3/86 adults, 2/59 children with chronic diarrhea (1 HIV+), 0/55 children with acute diarrhea, and 4/20 control children. All four controls were underweight (P < .01). Cyclospora was identified in specimens from one adult and one child with acute diarrhea (HIV-). Thus, Cryptosporidium was the most frequent and Cyclospora the least frequent pathogen identified. Cryptosporidium and Enterocytozoon were associated with malnutrition. Asymptomatic fecal shedding of Enterocytozoon in otherwise healthy, HIV children has not been described previously

Evolution of planetary nebulae II. Population effects on the bright cut-off of the PNLF

We investigate the bright cut-off of the [OIII]l5007 planetary nebula luminosity function (PNLF), that has been suggested as a powerful extragalactic distance indicator. Theoretical PNLFs are constructed via Monte-Carlo simulations of populations of PNe, whose individual properties are described with the aid of recent PN synthetic models (Marigo et al. 2001), coupled to a detailed photoionisation code (CLOUDY). The basic dependences of the cut-off magnitude M* are then discussed. We find that: (i) In galaxies with recent or ongoing star formation, the modelled PNLF present M* values between -4 and -5, very close to the observationally-calibrated value for the LMC. (ii) In these galaxies, the PNLF cut-off is produced by PNe with progenitor masses of about 2.5 Msun, while less massive stars give origin to fainter PNe. As a consequence M* is expected to depend strongly on the age of the last burst of star formation, dimming by as much as 5 mag as we go from young to 10-Gyr old populations. (iii) Rather than on the initial metallicity of a stellar population, M* depends on the actual [O/H] of the observed PNe, a quantity that may differ significantly from the initial value (due to dredge-up episodes), especially in young and intermediate-age PN populations. (iv) Also the transition time from the end of AGB to the PN phase, and the nuclear-burning properties (i.e. H- or He-burning) of the central stars introduce non-negligible effects on M*. The strongest indication derived from the present calculations is a serious difficulty to explain the age-invariance of the cut-off brightness over an extended interval, say from 1 to 13 Gyr, that observations of PNLFs in galaxies of late-to-early type seem to suggest.Comment: 22 pages, to appear in Astronomy & Astrophysic