Genome-wide association analysis of susceptibility and clinical phenotype in multiple sclerosis

Multiple sclerosis (MS), a chronic disorder of the central nervous system and common cause of neurological disability in young adults, is characterized by moderate but complex risk heritability. Here we report the results of a genome-wide association study performed in a 1000 prospective case series of well-characterized individuals with MS and group-matched controls using the Sentrix® HumanHap550 BeadChip platform from Illumina. After stringent quality control data filtering, we compared allele frequencies for 551 642 SNPs in 978 cases and 883 controls and assessed genotypic influences on susceptibility, age of onset, disease severity, as well as brain lesion load and normalized brain volume from magnetic resonance imaging exams. A multi-analytical strategy identified 242 susceptibility SNPs exceeding established thresholds of significance, including 65 within the MHC locus in chromosome 6p21.3. Independent replication confirms a role for GPC5, a heparan sulfate proteoglycan, in disease risk. Gene ontology-based analysis shows a functional dichotomy between genes involved in the susceptibility pathway and those affecting the clinical phenotyp

Compactación del suelo durante la cosecha forestal: evolución de las propiedades físicas Soil compaction by forestry harvester operation: evolution of physical properties

Se realizaron ensayos en campo con el objeto de evaluar los efectos y la persistencia de la compactación inducida por diferentes intensidades de tránsito durante la cosecha forestal en una población de álamos (Populus sp.). Se trabajó con un tractor de tracción simple de 73,5 kW y un acoplado, con una masa de 4,2 Mg y 2,7 Mg, respectivamente. Se establecieron 3 tratamientos de tránsito: 1, 5 y 10 pasajes sobre una misma senda, contrastados con un testigo sin tránsito. Luego de cada tratamiento, y 3 años después de realizados los mismos, se efectuaron determinaciones de penetrometría, densidad aparente y humedad gravimétrica. Hubo incrementos significativos de resistencia a la penetración para los tratamientos con tránsito, alcanzando el de mayor intensidad valores entre el 76 y el 16 % en los primeros 40 cm de profundidad. La densidad aparente tuvo una tendencia similar limitada a una menor profundidad. Luego de 3 años, los efectos persistían a nivel subsuperficial, para el índice de cono en los sectores tránsitados con respecto a los no transitados, encontrándose diferencias significativas para el tratamiento de 10 pasajes respecto al de 1 pasaje hasta 50 cm. Aún con cargas por eje de 3 Mg, el incremento de la intensidad de tránsito induce compactación a nivel subsuperficial que persiste en el tiempo.<br>The long-term effects on soil compaction by different traffic intensities during forestry harvester operation were evaluated in field experiments on heavy clay, vertic Argiudol. A conventional 2WD 73.5 kW engine power tractor with a trailer, 4.2 Mg and 3.75 Mg total load respectively, were used on a multiclonal Populus deltoides (Marsh) 3x3 plantation. There were four treatments: one pass, five pass and ten repeated passes with the tandem over the same track, and a control plot without experimental traffic. Moisture content, bulk density and penetrometer resistances were evaluated before and immediately after the traffic treatments and three years later. Effects of induced soil compaction by traffic were especially marked on topsoil, with cone index increments between 76 and 16% in the first 40 cm of the surface layer after treatments. Bulk density had a similar tendency but limited to a 15 cm depth. Three years later, only subsoil cone index in the track line, below 20 cm depth, was significantly higher than the interline track soil cone index. The 10 passes treatment had significant differences compared to the 1 pass and no traffic control plot treatments from the surface layer to 50 cm depth, without differences with the 5 passes treatment in most of the studied soil layers. Subsoil compaction may be induced by repeated traffic with low axle load and their effects persist for a very long time