Novel Roles for Selected Genes in Meiotic DNA Processing

High-throughput studies of the 6,200 genes of Saccharomyces cerevisiae have provided valuable data resources. However, these resources require a return to experimental analysis to test predictions. An in-silico screen, mining existing interaction, expression, localization, and phenotype datasets was developed with the aim of selecting minimally characterized genes involved in meiotic DNA processing. Based on our selection procedure, 81 deletion mutants were constructed and tested for phenotypic abnormalities. Eleven (13.6%) genes were identified to have novel roles in meiotic DNA processes including DNA replication, recombination, and chromosome segregation. In particular, this analysis showed that Def1, a protein that facilitates ubiquitination of RNA polymerase II as a response to DNA damage, is required for efficient synapsis between homologues and normal levels of crossover recombination during meiosis. These characteristics are shared by a group of proteins required for Zip1 loading (ZMM proteins). Additionally, Soh1/Med31, a subunit of the RNA pol II mediator complex, Bre5, a ubiquitin protease cofactor and an uncharacterized protein, Rmr1/Ygl250w, are required for normal levels of gene conversion events during meiosis. We show how existing datasets may be used to define gene sets enriched for specific roles and how these can be evaluated by experimental analysis

On the magnetic structure of the solar transition region

We examine the hypothesis that ``cool loops'' dominate emission from solar transition region plasma below temperatures of $2\times10^5$K. We compare published VAULT images of H L$\alpha$, a lower transition region line, with near-contemporaneous magnetograms from Kitt Peak, obtained during the second flight (VAULT-2) on 14 June 2002. The measured surface fields and potential extrapolations suggest that there are too few short loops, and that L$\alpha$ emission is associated with the base regions of longer, coronal loops. VAULT-2 data of network boundaries have an asymmetry on scales larger than supergranules, also indicating an association with long loops. We complement the Kitt Peak data with very sensitive vector polarimetric data from the Spectro-Polarimeter on board Hinode, to determine the influence of very small magnetic concentrations on our analysis. From these data two classes of behavior are found: within the cores of strong magnetic flux concentrations ($> 5\times10^{18}$ Mx) associated with active network and plage, small-scale mixed fields are absent and any short loops can connect just the peripheries of the flux to cell interiors. Core fields return to the surface via longer, most likely coronal, loops. In weaker concentrations, short loops can connect between concentrations and produce mixed fields within network boundaries as suggested by Dowdy and colleagues. The VAULT-2 data which we examined are associated with strong concentrations. We conclude that the cool loop model applies only to a small fraction of the VAULT-2 emission, but we cannot discount a significant role for cool loops in quieter regions. We suggest a physical picture for how network L$\alpha$ emission may occur through the cross-field diffusion of neutral atoms from chromospheric into coronal plasma.Comment: Accepted by ApJ, 9 May 200

Preliminary Report of a Hybrid Total Ankle Arthroplasty Combining a Stemmed Intramedullary Tibial Component With Chamfer-Cut Talar Dome.

Total ankle arthroplasty (TAA) is a viable treatment for end-stage ankle arthritis. In our experience, a stemmed intramedullary tibial component combined with a chamfer-cut talar component provides the most stable construct for TAA. We present our technique for placement of this hybrid prosthesis utilizing the INBONE tibial component in combination with the INFINITY talar component. This technique differs from the standard protocol by minimizing use of both patient-specific and standard intraoperative guides. The primary aim of this study is to report our preliminary outcomes with our novel technique. Secondarily, we aim to demonstrate that placement of this hybrid prosthesis is radiographically reproducible and accurate. The first 10 patients undergoing this technique with at least 1 year of follow-up were retrospectively reviewed. Average visual analog pain scale decreased from 7.4 preoperatively to 0.5 at 1 year postoperatively. The average time to weightbearing was 6.4 weeks. Complications were minimal, and no implant-related complications were encountered. First weightbearing ankle radiographs postoperatively were evaluated by 3 reviewers to determine accuracy of the tibial intramedullary stem in relation to the anatomical axis of the tibia. We found that the deviation of the tibial implant from the anatomic axis was on average 0.9°± 0.5° in the coronal plane, and 2.2°± 2.7° in the sagittal plane. Inter-rater reliability was 83%. We conclude that this hybrid technique utilizing a stemmed intramedullary tibial component in combination with a chamfer-cut talar component for TAA is reproducible, accurate, and safe

High Spatial Resolution Thermal-Infrared Spectroscopy with ALES: Resolved Spectra of the Benchmark Brown Dwarf Binary HD 130948BC

We present 2.9-4.1 micron integral field spectroscopy of the L4+L4 brown dwarf binary HD 130948BC, obtained with the Arizona Lenslets for Exoplanet Spectroscopy (ALES) mode of the Large Binocular Telescope Interferometer (LBTI). The HD 130948 system is a hierarchical triple system, in which the G2V primary is joined by two co-orbiting brown dwarfs. By combining the age of the system with the dynamical masses and luminosities of the substellar companions, we can test evolutionary models of cool brown dwarfs and extra-solar giant planets. Previous near-infrared studies suggest a disagreement between HD 130948BC luminosities and those derived from evolutionary models. We obtained spatially-resolved, low-resolution (R~20) L-band spectra of HD 130948B and C to extend the wavelength coverage into the thermal infrared. Jointly using JHK photometry and ALES L-band spectra for HD 130948BC, we derive atmospheric parameters that are consistent with parameters derived from evolutionary models. We leverage the consistency of these atmospheric quantities to favor a younger age (0.50 \pm 0.07 Gyr) of the system compared to the older age (0.79 \pm 0.22 Gyr) determined with gyrochronology in order to address the luminosity discrepancy.Comment: 17 pages, 9 figures, Accepted to Ap

Approaches to herbicide (MCPA) pollution mitigation in drinking water source catchments using enhanced space and time monitoring

Publication history: Accepted - 30 September 2020; Published online - 8 October 2020Freshwater occurrences of the selective acid herbicide 2-methyl-4-chloro-phenoxyacetic acid (MCPA) are an ongoing regulatory and financial issue for water utility industries as the number and magnitude of detections increase, particularly in surface water catchments. Assessments for mitigating pesticide pollution in catchments used as drinking water sources require a combination of catchment-based and water treatment solutions, but approaches are limited by a lack of empirical data. In this study, an enhanced spatial (11 locations) and temporal (7-hourly to daily sampling) monitoring approach was employed to address these issues in an exemplar surface water source catchment (384 km2). The spatial sampling revealed that MCPA was widespread, with occurrences above the 0.1 μg L−1 threshold for a single pesticide being highly positively correlated to sub-catchments with higher proportions of ‘Improved Grassland’ land use (r = 0.84). These data provide a strong foundation for targeting catchment-based mitigation solutions and also add to the debate on the ecosystems services provided by such catchments. Additionally, of the 999 temporal samples taken over 12 months from the catchment outlet, 25% were above the drinking water threshold of 0.1 μg L−1. This prevalence of high concentrations presents costly problems for source water treatment. Using these data, abstraction shutdowns were simulated for five scenarios using hydrometeorological data to explore the potential to avoid intake of high MCPA concentrations. The scenarios stopped abstraction for 4.2–9.3% of the April–October period and reduced intake of water containing over 0.1 μg L−1 of MCPA by 16–31%. This represents an important development for real-time proxy assessments for water abstraction in the absence of more direct pesticide monitoring data.This work was funded by the Source to Tap project (project reference IVA5018 – www.sourcetotap.eu). The Source to Tap project is supported by the European Union's INTERREG VA Programme, managed by the Special EU Programmes Body (SEUPB)

Evaluation of Chemcatcher® passive samplers for pesticide monitoring using high-frequency catchment scale data

Publication history: Accepted - 13 September 2022; Published online - 30 September 2022Passive samplers (PS) have been proposed as an enhanced water quality monitoring solution in rivers, but their performance against high-frequency data over the longer term has not been widely explored. This study compared the performance of Chemcatcher® passive sampling (PS) devices with high-frequency sampling (HFS: 7-hourly to daily) in two dynamic rivers over 16 months. The evaluation was based on the acid herbicides MCPA (2-methyl-4-chlorophenoxyacetic acid), mecoprop-P, fluroxypyr and triclopyr. The impact of river discharge parameters on Chemcatcher® device performance was also explored. Mixed effects modelling showed that time-weighted mean concentration (TWMC) and flow-weighted mean concentration (FWMC) values obtained by the HFS approach were both significantly higher (p 0.05). There was little indication that river flow parameters altered PS performance—some minor effects were not significant or consistent. Despite this, the PS recovery of very low concentrations indicated that Chemcatcher® devices may be used to evaluate the presence/absence and magnitude of acid herbicides in hydrologically dynamic rivers in synoptic type surveys where space and time coverage is required. However, a period of calibration of the devices in each river would be necessary if they were intended to provide a quantitative review of pesticide concentration as compared with HFS approaches.This work was funded in part by the Source to Tap project (project reference IVA5018 – http://www.sourcetotap.eu), supported by the European Union’s INTERREG VA Programme which is managed by the Special EU Programmes Body (SEUPB). The work was also part-funded by the FAIRWAY project (project reference 727984 - http://www.fair way-project.eu/). supported by the European Union’s HORIZON 2020 Programme

The connection of Type II Spicules to the Corona

We examine the hypothesis that plasma associated with "Type II" spicules is heated to coronal temperatures, and that the upward moving hot plasma constitutes a significant mass supply to the solar corona. 1D hydrodynamical models including time- dependent ionization are brought to bear on the problem. These calculations indicate that heating of field-aligned spicule flows should produce significant differential Doppler shifts between emission lines formed in the chromosphere, transition region, and corona. At present, observational evidence for the computed 60-90 km/s differential shifts is weak, but the data are limited by difficulties in comparing the proper motion of Type- II spicules, with spectral and kinematic properties of associated transition region and coronal emission lines. Future observations with the upcoming IRIS instrument should clarify if Doppler shifts are consistent with the dynamics modeled here.Comment: Astrophysical Journal, in pres

High Temperature Capacitive Pressure Sensor Employing a SiC Based Ring Oscillator

In an effort to develop harsh environment electronic and sensor technologies for aircraft engine safety and monitoring, we have used capacitive-based pressure sensors to shift the frequency of a SiC-electronics-based oscillator to produce a pressure-indicating signal that can be readily transmitted, e.g. wirelessly, to a receiver located in a more benign environment. Our efforts target 500 C, a temperature well above normal operating conditions of commercial circuits but within areas of interest in aerospace engines, deep mining applications and for future missions to the Venus atmosphere. This paper reports for the first time a ring oscillator circuit integrated with a capacitive pressure sensor, both operating at 500 C. This demonstration represents a significant step towards a wireless pressure sensor that can operate at 500 C and confirms the viability of 500 C electronic sensor systems

Ipl1/aurora kinase suppresses S-CDK-driven spindle formation during prophase I to ensure chromosome integrity during meiosis

Cells coordinate spindle formation with DNA repair and morphological modifications to chromosomes prior to their segregation to prevent cell division with damaged chromosomes. Here we uncover a novel and unexpected role for Aurora kinase in preventing the formation of spindles by Clb5-CDK (S-CDK) during meiotic prophase I and when the DDR is active in budding yeast. This is critical since S-CDK is essential for replication during premeiotic S-phase as well as double-strand break induction that facilitates meiotic recombination and, ultimately, chromosome segregation. Furthermore, we find that depletion of Cdc5 polo kinase activity delays spindle formation in DDR-arrested cells and that ectopic expression of Cdc5 in prophase I enhances spindle formation, when Ipl1 is depleted. Our findings establish a new paradigm for Aurora kinase function in both negative and positive regulation of spindle dynamics