Adjustable mount for electro-optic transducers in an evacuated cryogenic system

The invention is an adjustable mount for positioning an electro-optic transducer in an evacuated cryogenic environment. Electro-optic transducers are used in this manner as high sensitivity detectors of gas emission lines of spectroscopic analysis. The mount is made up of an adjusting mechanism and a transducer mount. The adjusting mechanism provided five degrees of freedom, linear adjustments and angular adjustments. The mount allows the use of an internal lens to focus energy on the transducer element thereby improving the efficiency of the detection device. Further, the transducer mount, although attached to the adjusting mechanism, is isolated thermally such that a cryogenic environment can be maintained at the transducer while the adjusting mechanism remains at room temperature. Radiation shields also are incorporated to further reduce heat flow to the transducer location

Granulomatous fasciitis followed by morphea profunda: Is granulomatous fasciitis part of a spectrum of deep morphea? A case report and review of the literature.

Although eosinophilic fasciitis is known to be part of the deep morphea spectrum, this first report of the coexistence of granulomatous fasciitis and morphea profunda suggests that granulomatous fasciitis may also be a part of the spectrum of deep morphea

Food insecurity and subsequent weight gain in women

Objective: Cross-sectional data indicate that a relationship between household food insecurity and overweight exists among women in the USA. Cross-sectional data cannot determine if food insecurity leads to overweight as some have hypothesised. The purpose of the present study was to examine the relationship of food insecurity with subsequent weight gain in women using data from the Panel Study of Income Dynamics (PSID). Design, setting and subjects:Panel data from the 1999 and 2001 PSID, a nationally representative sample of households, were analysed using multivariate regression procedures. Results: Average weight gain among all women (n=5595) was 1.1 kg on average over the two years. There were no significant differences in the percentages of women who gained a clinically significant amount (2.3kg) by food insecurity status. Overweight women who were on a weight-gain trajectory during the 2-year period gained less if they were food-insecure. This relationship was not observed among healthy-weight or obese women. Conclusions: Overall, food insecurity does not appear to be strongly associated with subsequent weight gain in women

Construction of a quasiconserved quantity in the Henon-Heiles problem using a single set of variables

The problem of finding the coefficients of a simple series expansion for a quasiconserved quantity K for the Henon-Heiles Hamiltonian H using a single set of variables is solved. In the past, this type of approach has been problematic because the solution to the equations determining the coefficients in the expansion is not unique. As a result, the existence of a consistent expression for K to all orders had not previously been established. We show how to deal with this arbitrariness in the expansion coefficients for K in a consistent way. Due to this arbitrariness, we find a class of expansions for K, in contrast to the single unique expansion for K generated by the normal-form approach of Gustavson [Astron. J. 71, 670 (1966)]. It may be possible to devise a criterion for deciding which one of our expansions is optimally convergent, although we do not deal with this question here. We proceed by introducing a single set of dynamic variables that have simple symmetry properties and that also diagonalize the problem of finding the coefficients of K. No canonical transformations are required. A straightforward constructive procedure is given for generating the power series to any order for quantities having the symmetry of the Hamiltonian that -are formally conserved. This leads to a very practical method for calculating a quasiconserved quantity in the Henon-Heiles problem. A comparison is made through several orders of the terms generated by this approach and those generated in the original Gustavson expansion in normal form

Phylogenetic relationships of the Wolbachia of nematodes and arthropods

Wolbachia are well known as bacterial symbionts of arthropods, where they are reproductive parasites, but have also been described from nematode hosts, where the symbiotic interaction has features of mutualism. The majority of arthropod Wolbachia belong to clades A and B, while nematode Wolbachia mostly belong to clades C and D, but these relationships have been based on analysis of a small number of genes. To investigate the evolution and relationships of Wolbachia symbionts we have sequenced over 70 kb of the genome of wOvo, a Wolbachia from the human-parasitic nematode Onchocerca volvulus, and compared the genes identified to orthologues in other sequenced Wolbachia genomes. In comparisons of conserved local synteny, we find that wBm, from the nematode Brugia malayi, and wMel, from Drosophila melanogaster, are more similar to each other than either is to wOvo. Phylogenetic analysis of the protein-coding and ribosomal RNA genes on the sequenced fragments supports reciprocal monophyly of nematode and arthropod Wolbachia. The nematode Wolbachia did not arise from within the A clade of arthropod Wolbachia, and the root of the Wolbachia clade lies between the nematode and arthropod symbionts. Using the wOvo sequence, we identified a lateral transfer event whereby segments of the Wolbachia genome were inserted into the Onchocerca nuclear genome. This event predated the separation of the human parasite O. volvulus from its cattle-parasitic sister species, O. ochengi. The long association between filarial nematodes and Wolbachia symbionts may permit more frequent genetic exchange between their genomes

IUPHAR-DB: An Expert-Curated, Peer-Reviewed Database of Receptors and Ion Channels

The International Union of Basic and Clinical Pharmacology database (IUPHAR-DB) integrates peer-reviewed pharmacological, chemical, genetic, functional and anatomical information on the 354 non-sensory G protein-coupled receptors (GPCRs), 71 ligand-gated ion channel subunits and 141 voltage-gated ion channel subunits encoded by the human, rat and mouse genomes. These genes represent the targets of about a third of currently approved drugs and are a major focus of drug discovery and development programs in the pharmaceutical industry. Individual gene pages provide a comprehensive description of the genes and their functions, with information on protein structure, ligands, expression patterns, signaling mechanisms, functional assays and biologically important receptor variants (e.g. single nucleotide polymorphisms and splice variants). The phenotypes resulting from altered gene expression (e.g. in genetically altered animals) and genetic mutations are described. Links are provided to bioinformatics resources such as NCBI RefSeq, OMIM, PubChem, human, rat and mouse genome databases. Recent developments include the addition of ligand-centered pages summarising information about unique ligand molecules in IUPHAR-DB. IUPHAR-DB represents a novel approach to biocuration because most data are provided through manual curation of published literature by a network of over 60 expert subcommittees coordinated by NC-IUPHAR. Data are referenced to the primary literature and linked to PubMed. The data are checked to ensure accuracy and consistency by the curators, added to the production server using custom-built submission tools and peer-reviewed by NC-IUPHAR, before being transferred to the public database. Data are reviewed and updated regularly (at least biennially). Other website features include comprehensive database search tools, online and downloadable gene lists and links to recent publications of interest to the field, such as reports on receptor-ligand pairings. The database is freely available at "http://www.iuphar-db.org":http://www.iuphar-db.org. Curators can be reached at curators [at] iuphar-db.org. We thank British Pharmacological Society, UNESCO (through the ICSU Grants Programme), Incyte, GlaxoSmithKline, Novartis, Servier and Wyeth for their support

Modulation of LPS stimulated NF-kappaB mediated Nitric Oxide production by PKCε and JAK2 in RAW macrophages

<p>Abstract</p> <p>Background</p> <p>Nuclear factor kappa B (NF-κB) has been shown to play an important role in regulating the expression of many genes involved in cell survival, immunity and in the inflammatory processes. NF-κB activation upregulates inducible nitric oxide synthase leading to enhanced nitric oxide production during an inflammatory response. NF-κB activation is regulated by distinct kinase pathways independent of inhibitor of κB kinase (IKK). Here, we examine the role of protein kinase C isoforms and janus activated kinase 2 (JAK2) activation in NF-κB activation and LPS-stimulated NO production.</p> <p>Methods</p> <p>Murine RAW 264.7 macrophages were treated with lipopolysaccharide (LPS), Phorbol 12-myristate 13-acetate (PMA) and a combination of LPS and PMA in the presence or absence of various inhibitors of PKC isoforms and JAK2. Nuclear translocation of the NF-κB p65 subunit, was assessed by Western blot analysis whilst NO levels were assessed by Greiss assay.</p> <p>Results</p> <p>LPS-stimulated NO production was attenuated by PMA whilst PMA alone did not affect NO release. These effects were associated with changes in p65 nuclear translocation. The PKCα, β, γ, δ and ζ inhibitor Gö 6983 (Go) had no effect on LPS-induced NO release. In contrast, Bisindolymalemide I (Bis), a PKC α, β_I, β_II, γ, δ and ε isoform inhibitors completely inhibited LPS-stimulated NO production without affecting p65 nuclear translocation. Furthermore, a partial inhibitory effect on LPS-induced NO release was seen with the JAK2 inhibitor AG-490 and the p38 MAPK inhibitor SB 203850.</p> <p>Conclusion</p> <p>The results further define the role of NF-κB in LPS stimulated NO production in RAW macrophages. The data support a function for PKCε, JAK2 and p38 MAPK in NF-κB activation following p65 nuclear import.</p

First Simultaneous Optical and EUV Observations of the Quasi-Coherent Oscillations of SS Cygni

Using EUV photometry obtained with the Extreme Ultraviolet Explorer (EUVE) satellite and UBVR optical photometry obtained with the 2.7-m telescope at McDonald Observatory, we have detected quasi-coherent oscillations (so-called ``dwarf nova oscillations'') in the EUV and optical flux of the dwarf nova SS Cygni during its 1996 October outburst. There are two new results from these observations. First, we have for the first time observed ``frequency doubling:'' during the rising branch of the outburst, the period of the EUV oscillation was observed to jump from 6.59 s to 2.91 s. Second, we have for the first time observed quasi-coherent oscillations simultaneously in the optical and EUV. We find that the period and phase of the oscillations are the same in the two wavebands, finally confirming the long-held assumption that the periods of the optical and EUV/soft X-ray oscillations of dwarf novae are equal. The UBV oscillations can be simply the Rayleigh-Jeans tail of the EUV oscillations if the boundary layer temperature kT_bb <~ 15 eV and hence the luminosity L_bb >~ 1.2e34 (d/75 pc)^2 erg/s (comparable to that of the accretion disk). Otherwise, the lack of a phase delay between the EUV and optical oscillations requires that the optical reprocessing site lies within the inner third of the accretion disk. This is strikingly different from other cataclysmic variables, where much or all of the disk contributes to the optical oscillations.Comment: 16 pages including 3 tables and 4 encapsulated postscript figures; LaTeX format, uses aastex.cls; accepted on 2001 August 2 for publication in The Astrophysical Journa

Age and Growth of Spotted Sand Bass, Paralabrax maculatofasciatus, in Bahia de Los Angeles, Baja California, Mexico, with Age Validation using Otolith Edge Analysis

Spotted sand bass, Paralabrax maculatofasciatus, were collected from Bahia de Los Angeles, Baja California, Mexico covering as wide a size range as possible over four seasons (spring, summer, fall, and winter). Age was estimated and growth parameters calculated from growth zones counted in transverse otolith sections. An otolith edge analysis indicated an opaque growth zone was deposited once per year during the summer, validating the annual periodicity. Spotted sand bass from this region are fast growing with a relatively short life span of up to 11 years. Growth differs from the disjunct Pacific coast population by having a higher growth rate and a shorter longevity