Optimal team composition for tool‐based problem solving

In this paper, we construct a framework for modeling teams of agents who apply techniques or procedures (tools) to solve problems. In our framework, tools differ in their likelihood of solving the problem at hand; agents, who may be of different types, vary in their skill at using tools. We establish baseline hiring rules when a manager can dictate tool choice and then derive results for strategic tool choice by team members. We highlight three main findings: First, that cognitively diverse teams are more likely to solve problems in both settings. Second, that teams consisting of types that master diverse tools have an indirect strategic advantage because tool diversity facilitates coordination. Third, that strategic tool choice creates counterintuitive optimal hiring practices. For example, optimal teams may exclude the highest ability types and can include dominated types. In addition, optimal groups need not increase setwise. Our framework extends to cover teamwork on decomposable problems, to cases where individuals apply multiple tools, and to teams facing a flow or set of problems.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/152035/1/jems12295.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/152035/2/jems12295_am.pd

Optimized cDNA libraries for virus-induced gene silencing (VIGS) using tobacco rattle virus

<p>Abstract</p> <p>Background</p> <p>Virus-induced gene silencing (VIGS) has emerged as a method for performing rapid loss-of-function experiments in plants. Despite its expanding use, the effect of host gene insert length and other properties on silencing efficiency have not been systematically tested. In this study, we probed the optimal properties of cDNA fragments of the <it>phytoene desaturase </it>(<it>PDS</it>) gene for efficient VIGS in <it>Nicotiana benthamiana </it>using tobacco rattle virus (TRV).</p> <p>Results</p> <p><it>NbPDS </it>inserts of between 192 bp and 1304 bp led to efficient silencing as determined by analysis of leaf chlorophyll a levels. The region of the <it>NbPDS </it>cDNA used for silencing had a small effect on silencing efficiency with 5' and 3' located inserts performing more poorly than those from the middle. Silencing efficiency was reduced by the inclusion of a 24 bp poly(A) or poly(G) homopolymeric region. We developed a method for constructing cDNA libraries for use as a source of VIGS-ready constructs. Library construction involved the synthesis of cDNA on a solid phase support, digestion with RsaI to yield short cDNA fragments lacking poly(A) tails and suppression subtractive hybridization to enrich for differentially expressed transcripts. We constructed two cDNA libraries from methyl-jasmonate treated <it>N. benthamiana </it>roots and obtained 2948 ESTs. Thirty percent of the cDNA inserts were 401–500 bp in length and 99.5% lacked poly(A) tails. To test the efficiency of constructs derived from the VIGS-cDNA libraries, we silenced the nicotine biosynthetic enzyme, putrescine <it>N</it>-methyltransferase (<it>PMT</it>), with ten different VIGS-<it>NbPMT </it>constructs ranging from 122 bp to 517 bp. Leaf nicotine levels were reduced by more than 90% in all plants infected with the <it>NbPMT </it>constructs.</p> <p>Conclusion</p> <p>Based on the silencing of <it>NbPDS </it>and <it>NbPMT</it>, we suggest the following design guidelines for constructs in TRV vectors: (1) Insert lengths should be in the range of ~200 bp to ~1300 bp, (2) they should be positioned in the middle of the cDNA and (3) homopolymeric regions (i.e. poly(A/T) tails) should not be included. Our VIGS-cDNA library method, which incorporates these guidelines to produce sequenced, VIGS-ready cDNAs, will be useful for both fast-forward and reverse genetics experiments in TRV vectors.</p

Effectiveness of common household cleaning agents in reducing the viability of human influenza A/H1N1

In the event of an influenza pandemic, the majority of people infected will be nursed at home. It is therefore important to determine simple methods for limiting the spread of the virus within the home. The purpose of this work was to test a representative range of common household cleaning agents for their effectiveness at killing or reducing the viability of influenza A virus

Independent Domestication of Two Old World Cotton Species

Domesticated cotton species provide raw material for the majority of the world\u27s textile industry. Two independent domestication events have been identified in allopolyploid cotton, one in Upland cotton ( Gossypium hirsutum L.) and the other to Egyptian cotton ( Gossypium barbadense L.). However, two diploid cotton species, Gossypium arboreum L. and Gossypium herbaceum L., have been cultivated for several millennia, but their status as independent domesticates has long been in question. Using genome resequencing data, we estimated the global abundance of various repetitive DNAs. We demonstrate that, despite negligible divergence in genome size, the two domesticated diploid cotton species contain different, but compensatory, repeat content and have thus experienced cryptic alterations in repeat abundance despite equivalence in genome size. Evidence of independent origin is bolstered by estimates of divergence times based on molecular evolutionary analysis of f7,000 orthologous genes, for which synonymous substitution rates suggest that G. arboreum and G. herbaceum last shared a common ancestor approximately 0.4–2.5 Ma. These data are incompatible with a shared domestication history during the emergence of agriculture and lead to the conclusion that G. arboreum and G. herbaceum were each domesticated independently

The draft genome and transcriptome of Cannabis sativa

Background: Cannabis sativa has been cultivated throughout human history as a source of fiber, oil and food, and for its medicinal and intoxicating properties. Selective breeding has produced cannabis plants for specific uses, including high-potency marijuana strains and hemp cultivars for fiber and seed production. The molecular biology underlying cannabinoid biosynthesis and other traits of interest is largely unexplored. Results: We sequenced genomic DNA and RNA from the marijuana strain Purple Kush using shortread approaches. We report a draft haploid genome sequence of 534 Mb and a transcriptome of 30,000 genes. Comparison of the transcriptome of Purple Kush with that of the hemp cultivar 'Finola' revealed that many genes encoding proteins involved in cannabinoid and precursor pathways are more highly expressed in Purple Kush than in 'Finola'. The exclusive occurrence of \u3949-tetrahydrocannabinolic acid synthase in the Purple Kush transcriptome, and its replacement by cannabidiolic acid synthase in 'Finola', may explain why the psychoactive cannabinoid \u3949-tetrahydrocannabinol (THC) is produced in marijuana but not in hemp. Resequencing the hemp cultivars 'Finola' and 'USO-31' showed little difference in gene copy numbers of cannabinoid pathway enzymes. However, single nucleotide variant analysis uncovered a relatively high level of variation among four cannabis types, and supported a separation of marijuana and hemp. Conclusions: The availability of the Cannabis sativa genome enables the study of a multifunctional plant that occupies a unique role in human culture. Its availability will aid the development of therapeutic marijuana strains with tailored cannabinoid profiles and provide a basis for the breeding of hemp with improved agronomic characteristics.Peer reviewed: YesNRC publication: Ye

Thermodynamics with long-range interactions: from Ising models to black-holes

New methods are presented which enables one to analyze the thermodynamics of systems with long-range interactions. Generically, such systems have entropies which are non-extensive, (do not scale with the size of the system). We show how to calculate the degree of non-extensivity for such a system. We find that a system interacting with a heat reservoir is in a probability distribution of canonical ensembles. The system still possesses a parameter akin to a global temperature, which is constant throughout the substance. There is also a useful quantity which acts like a {\it local temperatures} and it varies throughout the substance. These quantities are closely related to counterparts found in general relativity. A lattice model with long-range spin-spin coupling is studied. This is compared with systems such as those encountered in general relativity, and gravitating systems with Newtonian-type interactions. A long-range lattice model is presented which can be seen as a black-hole analog. One finds that the analog's temperature and entropy have many properties which are found in black-holes. Finally, the entropy scaling behavior of a gravitating perfect fluid of constant density is calculated. For weak interactions, the entropy scales like the volume of the system. As the interactions become stronger, the entropy becomes higher near the surface of the system, and becomes more area-scaling.Comment: Corrects some typos found in published version. Title changed 22 pages, 2 figure

New Kansas Roots for Students: building cultural competency through the Nicodemus Project

Five-member panel (two faculty members representing two supporting professional disciplines; Nicodemus resident and on campus resource; a MLA graduate student; and a graduate planner) recapping how the Parks for the People/Nicodemus project transformed students and community members. Short segments of video demonstrating student learning outcomes associated with diversity and collaboration will be introduced. This project won the CECD Engagement Award from Kansas State University in 2013. (270-word abstract uploaded

Improved algebraic cryptanalysis of QUAD, Bivium and Trivium via graph partitioning on equation systems

We present a novel approach for preprocessing systems of polynomial equations via graph partitioning. The variable-sharing graph of a system of polynomial equations is defined. If such graph is disconnected, then the corresponding system of equations can be split into smaller ones that can be solved individually. This can provide a tremendous speed-up in computing the solution to the system, but is unlikely to occur either randomly or in applications. However, by deleting certain vertices on the graph, the variable-sharing graph could be disconnected in a balanced fashion, and in turn the system of polynomial equations would be separated into smaller systems of near-equal sizes. In graph theory terms, this process is equivalent to finding balanced vertex partitions with minimum-weight vertex separators. The techniques of finding these vertex partitions are discussed, and experiments are performed to evaluate its practicality for general graphs and systems of polynomial equations. Applications of this approach in algebraic cryptanalysis on symmetric ciphers are presented: For the QUAD family of stream ciphers, we show how a malicious party can manufacture conforming systems that can be easily broken. For the stream ciphers Bivium and Trivium, we nachieve significant speedups in algebraic attacks against them, mainly in a partial key guess scenario. In each of these cases, the systems of polynomial equations involved are well-suited to our graph partitioning method. These results may open a new avenue for evaluating the security of symmetric ciphers against algebraic attacks