Identification and density of ichthyoplankton in Laft Creeks (Hormuzgan province)

During a series of Ichthyoplankton survey from May 1998 to April 1999 Ichthyoplankton samples were collected at 12 stations in Laft creeks by 500 micron bongonet with oblique towing from bottom to water surface. Twenty-two families Ichthyoplanktons were identified (and two unidentified) during 12 months. Clupeiidae (31, 32%), gobiidae (26, 71%), scianidae (16, 55%), leiognathidae (11, 9%) were common and dominant families in all sampling stations. The creeks was divided in two branches: In east branch gobiidae (44.75%), clupeiidae (17.42%), scianida (13.5%) were more abundant than other families and in west branch clupeiidae (34.22%), gobiidae (23.77%), scianidae (16.34%) showed leiognathidae (13.26%) more abundance than other families, too. Some families e.g hemiramphidae, engrauilidae, blenidae, bothidae only occur in west branch, pleuronectidae family only occur in west branch. The highest density was found in winter season

Decreased expression of heat shock protein HSP90α after exposure to doxorubicin in breast cancer cell lines (MCF-7 and MDA-MB-231)

Background and purpose: Incidence of breast cancer is increasing day by day. Scientists are interested in the effects of inhibition of breast cancer cell on treatment of this cancer. The aim of this study was to determine IC50 of doxorubicin in 24 hours on cell lines MCF-7 and MDA-MB-231 and the expression of heat shock protein HSP90α as a factor in the cell before and after 24 hours exposure to doxorubicin in both breast cancer cell lines. Materials and methods: A descriptive interventional study was done in two cell lines MCF-7 and MDA-MB-231` after 24 hours exposure to doxorubicin. Sensitivity of cells to doxorubicin was determined using MTT Assay in excel software. HSP90α heat shock protein expression were qualitatively compared in both cell lines before and after exposure to doxorubicin using immunofluorescent techniques (Immunocytochemistry). Results: MTT Assay showed that IC50 value in MDA-MB-231 and MCF7 cells after 24 hours exposure to doxorubicin (the dose that kills 50% of cells) were 14.521 and 16.3315µM, respectively. Immunocytochemistry revealed that HSP90α protein expression in both cell lines after exposure to doxorubicin decreased compared to the control group. Conclusion: Cell density in cell lines (ER-) MDA-MB-231 and line MCF-7 (ER+) after exposure to doxorubicin and increasing the dose of medication, decreased indicating a dose dependent effect. Also, apoptosis occurred in both cell lines and expression of HSP90α decreased but MDA-MB-231 cells were found to be more sensitive. © 2017, Mazandaran University of Medical Sciences. All rights reserved

A survey of algal bloom impacts on abundance and distribution of macro benthos in Hormozgan province coastal waters

The investigation of the algal bloom effects on Macro benthos abundance and diversity was seasonally carried out in coastline waters of Hormozgan province in 1391. Fourteen stations were selected for sampling; as 8 stations in coast of Bandar Abbas, 3 in Jask Port (east of Hormozgan) and 3 stations in Lengeh Port (west of Hormozgan). Polychaets, oligochaets, mollusks, crustacean and others like nematodes, nemertean’s, foraminifera’s, and ophiuroides were identified. Crustacean with 333950 ind.^-2 were dominant group whereas nemertean with 5050 ind.^-2 were minimum in density. Results showed that mean abundance of macro benthos was at most in spring (5614±3992 end) ^-2 and with 1244±380 ind.-2 was at least in autumn. Related results to stations showed that mean abundance in Jask Port and Lengeh Port were more than Bandar Abbas City. Among 8 stations in Bandar Abbas, Langargah with 168±977 ind.^ -2 was at most and Posht-e- shahr was at least in abundance (82±256 ind. ^-2). In Lengeh and Jask ports the stations far from coasts showed more abundance than station near coast. Monthly investigation showed that Macro benthos community was much more in April with 1011±6783 ind.-2 than December with 6±28 ind.^-2 , respectively. Shannon -Winner diversity index was low (0.61.2) in three stations, Evenness ranged from 0.1 to 0.4 and Margalof ranged from 2 to 4. Among four stations, mean wet weight varied from 12.18±6/95 gr in Jask Port (maximum) to 6.24±3/85gr in Bandar Abbas (minimum) .Seasonally, in spring the wet weight of macro benthos was 15.15±16/32 (maximum) and with 1.43±0.88 gr showed the minimum value in summer. There was a negative correlation between silt and macro benthos abundance and a positive correlation existed between macro benthos community and sand. There was no significant correlation between macro benthos community and TOM. To pay attention to results like diversity, abundance, wet biomass and correlation; it seems that algal bloom has no obvious negative effect on Macro benthos communities

Constipation and laxative herbs in Iranian traditional medicine

Context: Constipation is one of the most prevalent gastrointestinal disorders and is characterized by stiffness of waste and difficult defecation. In modern medicine, different laxative drugs are used for the treatment of constipation, but they are not without side effects. Nonetheless, the appropriate use of herbal medicines can be beneficial for treating this disorder. Therefore, this study attempted to draw together some pharmacological and medical insights into the management of constipation through the use of Iranian traditional medicine (ITM). Evidence Acquisition: In this review article, we initially researched constipation and laxative herbs using some of the most famous ancient medical and pharmacological textbooks pertaining to Iranian medicine written from the 10th to 18th century AD. This includes The canon of medicine by Avicenna (10th and 11th centuries, Beirut publication), Makhzan-ol-advie by Aghili (18th century, Tehran University of Medical Science Press), and Al-shamel by Gharashi (13th century, Caltural foundation Publication). Then we extracted the names and laxative mechanisms of some herbs prescribed for constipation through ITM. Finally, a search of scientific databases such as PubMed and Google Scholar was performed to find related works concerning laxative mechanisms. Results: In ITM doctrine, laxatives are weak purgatives that lead to the defecation of waste from the gastrointestinal tract. From this perspective, laxative herbs can be classified into two groups based on their nature: laxative herbs with a hot nature cause the dilution and flowing of humors, but herbs with a cold nature function as laxatives due to their mucilage properties. Each of the laxatives was used in accordance with the primary reason for constipation. Conclusions: This study presents medical and pharmacological approaches in ITM for dealing with complications arising from constipation. Considering the optimistic results, these herbs can be used as a means of constipation management and further investigation into this area can potentially lead to the formulation of new drugs. © 2016, Iranian Red Crescent Medical Journal

Differential expression of HSP90β in MDA-MB-231 and MCF-7 cell lines after treatment with doxorubicin

Breast cancer is a complex, heterogeneous disease and one of the most common malignancies in women worldwide. The efficacy of chemotherapy as an important breast cancer treatment option has been severely limited because of the inherent or acquired resistance of cancer cells. The molecular chaperone heat shock protein 90 (HSP90) upregulated in response to cellular stress is required for functions such as conformational maturation, activation and stability in more than 200 client proteins, mostly of the signaling type. In this study, the expression of HSP90 isoforms including HSP90α and HSP90β in breast cancer cell lines before and after treatment with doxorubicin (DOX) was assessed. Material and Methods: The cell cytotoxicity of DOX in MDA-MB-231 and MCF-7 cell lines was determined using the MTT assay. Immunofluorescence and western blotting techniques were used to determine the expression of HSP90β in the cell lines before and after DOX treatment. Immunofluorescence was also conducted to ascertain the expression of HSP90α. Results: The MTT assay results showed that the MDAMB- 231 cells (IC50=14.521 μM) were more sensitive than the MCF-7 cells (IC50=16.3315 μM) to DOX. The immunofluorescence results indicated that the expression of HSP90α in both cell lines decreased after exposure to DOX. The western blot and immunofluorescence analyses showed that HSP90β expression decreased in the MCF-7 cells but increased in the MDAMB- 231 cells after DOX treatment. Conclusion: The obtained results suggested that HSP90α and HSP90β expression levels were reduced in the MCF-7 cells after exposure to DOX. In the MDA-MB-231 cells, HSP90α expression was reduced while HSP90β was found to be overexpressed following DOX treatment. © 2019 Korean Pharmacopuncture Institute

A survey on planktonic communities in Bandar Abbas coastal area

This study was conducted for investigation on algae bloom monitoring and oscillations of biotic and non-biotic parameters in coastal waters of Bandar Abbas from October 2013 to Septembre 2014. In this project, planktonic distribution and environmental data were collected from five stations, every 10 days. Four order and 45 genus of phytoplankton (Bacilliarophycea 29 genous, Dinophycea 13 genus, Cyanophycea 2 genus, Raphidophycea 1 genus) were identified, as well as , 10 order of zooplanckton consisted of Copepoda, Mollusca, Chaetognatha, Sarcomastigophora, Urochordata, Annalida, Nematoda, Echinodermata, Ciliophora and Granuloretica. Results showed that Bacillariophycea were dominate among phytoplankton species in all season. So that, Leptocylindrus, Chaetoceros and Nitzschia from Bacillarriophycea, Protoperidinium and Gymnodinium belong to Dinophycea, and Oscillatoria belong to Cyanophycea were dominate genus in Bandar Abbas coastal waters. The maximum and minimum density and frequency for phytoplankton were recorded respectively from Shilat Jety and Airport Stations. Based on ANOVA analysis on phytoplankton density no significant were observed seasonally (p>0.05), but significant differences were observed between stations (p<0.05). Algal blooms were recorded for Protoperidinum, Chaetoceros and Noctiluca form Shilat Jety in the study period. Result showed that Crustacea have more density amongst zooplankton community, although Arthropoda were dominate in all season. The maximum frequencies were recorded for Nauplius (78%) and Copepoda (21%), also Among copepod, Calanoida order observed 68.5% frequency in the study period. The ANOVA analysis showed significant differences both in stations and seasons for zooplankton density (p<0.05). The seasonal range of temperature were recorded between (19.43±2.58 - 31.98±2.48 ), salinity (27.15±9.48 - 38.13± 0.72ppt), dissolved Oxygen (2.8±1.81-6.87±1.25) mg/L, and pH (8.06±0.49- 8.69±0.23). Evaluation of biotic and abiotic factors indicate the probability of unhealthy and unstable Shilat jety and Gorzoozan stations coastal waters of Bandar Abbas City. One of these cases can be mentioned to high density of cyanophyceae in the estuary Gorzoozan compared other stations , high density of Phytoplankton and low diversity in the Shilat station. While the increase in Harpacticoida order of copepods As an indicator of pollution than on previous reports of other researchers In the this area, suggests pollution is pushed into the area

Hydrobiological studies on selected creeks in east Hormozgan for shrimp culture pond purpose

The coastal ecosystem, which represents the boundary in between pelagic province and land, is a complex one. Since most probes of physico-chemical characteristic in coastal ecosystem are unstable, it affects the distribution and abundance of the biota in it. The aim of this project was to determine Hydro-biological studies on the important creeks of East Hormozgan for shrimp culture pond purpose. Three creeks was selected on east coast namely as (Azini, Jask and Khalasi) and three stations in every creek which situated at the mouth, middle and end of the creek. The work in the project is incorporated in living and nonliving characteristics; the sampling period was in four seasons of 1384. Water characteristic like temperature, pH, dissolved oxygen, biochemical oxygen demand (B.O.D), salinity, carbon dioxide(Co2), nitrate, nitrite, ammonia, phosphate, silicate, total dissolved solid (T.D.S), total suspended particle,(T.S.S) and hydrogen sulphide (H2s). The recorded mean of these parameters in Azini creek (25.5±1.05 c), (8.11±0.13), (7.21±0.82 mg/l), (2.3±0.73 mg/l), (38.07± 0.86 ppt), (12.4±1.8 mg/l), (3.12±2.8 μg/l), (0.27±0.19 μg/l), (1.39±0.99 μg/l), (0.27±0.22 μg/l),(9.78±5.36 μg/l), (28.2±2.1 g/l), (37.6±11 mg/l),(0.1±0.07 mg/l), Jask creek (27.5±1.1 c ), (7.96±0.33), (6.79±0.77 mg/l), (2.9±0.96 mg/l), (38.77± 1.68 ppt), (10.8±3.4 mg/l), (3.51±2.2μg/l), (0.2±0.15 μg/l), (2.13±1.48 μg/l), (0.55±0.42 μg/l), (13±6.3 μg/l), (38.8±1.9 g/l), (37.1±13 mg/l),(0.32±0.28 mg/l), and Khalasi creek (28.2±1.9 c), (8.04±0.16), (6.9±0.48 mg/l), (2.22±1.03 mg/l), (38.79± 1.46 ppt), (12 ±1.1 mg/l), (3.08±1.4 μg/l), (0.2±0.14 μg/l), (1.41±0.65 μg/l), (0.45±0.39 μg/l), (13.3±4.2 μg/l), (38±3.5 g/l), (35.3±9 mg/l),(0.08±0.04 mg/l), respectively. The range of Total Organic Matter and Total Phosphor of sediment in Azini creek recorded as (4.99-10.57), (0.4-1.73), Jask creek (2.57-5.89), (0.0-1.99) and Khalasi creek (5.23-6.89), (0.59-1.09) respectively. Comparison of concentration mean between three creek shows there was significant difference between Temperature , pH, Dissolved Oxygen, Salinity, free Carbon dioxide, Silicate, Phytoplankton and Zooplankton (P<0.05. Phytoplankton with (31 genera from 3 families),(34genera from 4 families) and (38 genera from 3 families) were recorded in Azini, Jask and Khalasi creeks respectively. Zooplankton with 12, 12, and 10 group was present in Azini, Jask and Khalasi creeks. Macrofauna with (54 species from 38 families and 20 Order), (35species from 25 families and 17 Order) and (51 149 species from 39 families and 20 Order) were recorded from Azini, Jask and Khalasi creeks respectively. Minimum and Maximum density of phytoplankton in Azini, Jask and Khalasi creeks were (63-22914), (68-94320) and (280-5523) in liter, Zooplankton (193048-226337), (36750-713753) and (32625-489532) in m^3 and Macrofauna (6400-11357), (8375-24800), and (900-12473) in m^2, Total Count (90-3400), (70-22150), and (0-2250) in one gram sediment and Total Vibrio (43-2400), (15-2400) and (25-460) in one gram sediment respectively

Laboratories risks evaluation of Persian Gulf and Oman Sea Ecological Research Center

In this study, to assess and classify risks associated with working in the laboratories of Persian Gulf and Oman Sea Ecological Research Center, the method of "Failure Mode Effects Analysis" (FMEA) as well as some statistical methods were used. The results of the risk assessment in the 11 affiliated laboratories showed that the risk levels in all cases, except for benthos laboratory, could be evaluated as moderate or high and therefore appropriate corrective actions must be implemented. Based on the results of the Kruskal-Wallis tests both before and after the corrective actions, there were significant differences between the laboratories from the viewpoint of risk priority number (RPN). The post hoc tests showed the lowest risk levels for the benthose and histology laboratories, while the highest risks identified in the laboratory of instrumental analysis. The results of the classification of the laboratories using cluster analysis are largely similar to those of the posthoc tests. According to Mann-Whitney U test, only in the case of the samples preparation laboratory, significant differences between the values of the RPN before and after the corrective actions could be observed (p> 0.05), however, the risk levels still remained high. In general it can be concluded that FMEA is an effective method for risk assessment in the research laboratories and appropriate statistical methods can also be used for complementary analysis