Coach and athlete perceptions of ambiguous behaviors and sexual harassment

The purpose of this study was to examine the perceptions of coaches and athletes of ambiguous behaviors (i.e. actions which may or may not be construed as sexual harassment) in order to inform curriculum development in coach education. This study replicates and extends a previous American study which examined perceptions and experiences of sexual harassment among 210 female college athletes in the USA (Volkwein, et al., 1997). The Americans’ research design was based on earlier work in education by Garlick (1994). The present study is the first, large-scale UK survey of sexual harassment among student athletes (n = 311) and coaches (n = 182). A sexual harassment questionnaire (Garlick, 1994) was adapted to a sport setting and UK context. The questionnaire contained statements regarding 19 ambiguous behaviors. Factor analysis identified four subcategories of the questionnaire: Invitations/1-to-1; Social enquiries; Invasion of personal space; Personal Enquiries. A 2 x 2 (Gender by Role) MANCOVA, with age as a covariate revealed no interaction effect (Wilk’s = .99, F (4, 481) = 1.46, p > .05), but both main effects were significant (gender Wilk’s = .93, F (4, 481) = 8.62, p < .001, role Wilk’s = .956, F (4, 481) = 5.56, p < .001). Further analysis confirmed that athletes rated each of the four sub-scales as significantly more appropriate than coaches. Females rated social enquiries and invasion of personal space as more appropriate than males

Regulation of pituitary MT1 melatonin receptor expression by gonadotrophin-releasing hormone (GnRH) and early growth response factor-1 (Egr-1) : in vivo and in vitro studies

Copyright: © 2014 Bae et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This work was funded by the UK Biotechnology and Biological Sciences Research Council (BBSRC; grant BB/F020309/1; http://www.bbsrc.ac.uk/home/home.aspx). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer reviewedPublisher PD

Novel perspectives gained from new reconstruction algorithms

Since the 1970s, much of traditional interferometric imaging has been built around variations of the CLEAN algorithm, in both terminology, methodology, and algorithm development. Recent developments in applying new algorithms from convex optimization to interferometry has allowed old concepts to be viewed from a new perspective, ranging from image restoration to the development of computationally distributed algorithms. We present how this has ultimately led the authors to new perspectives in wide-field imaging, allowing for the first full individual non-coplanar corrections applied during imaging over extremely wide-fields of view for the Murchison Widefield Array (MWA) telescope. Furthermore, this same mathematical framework has provided a novel understanding of wide-band polarimetry at low frequencies, where instrumental channel depolarization can be corrected through the new δ λ2 -projection algorithm. This is a demonstration that new algorithm development outside of traditional radio astronomy is valuable for the new theoretical and practical perspectives gained. These perspectives are timely with the next generation of radio telescopes coming online

Effect of short chain fatty acids on the expression of free fatty acid receptor 2 (Ffar2), Ffar3 and early-stage adipogenesis

Adipose tissue has a major influence on insulin sensitivity. Stimulation of free fatty acid receptor 2 (FFAR2) has been proposed to influence adipocyte differentiation. We hypothesised that exposing preadipocytes to short chain fatty acids would induce earlier expression of nuclear receptors that co-ordinate adipogenesis, triglyceride accumulation and leptin secretion. 3T3-L1 preadipocytes were differentiated in the presence of 1 μM acetate, 0.1–10 μM propionate or vehicle control. In experiment 1, expression of Ffar2 and nuclear receptor mRNA was measured by quantitative PCR over 48 h following onset of differentiation. In experiment 2, extracellular leptin concentration and intracellular triglyceride content were measured at days 0, 2, 4, 6, 8 and 10 following the onset of differentiation. Control cells exhibited similar temporal dynamics of gene expression, triglyceride accumulation and leptin secretion as reported previously. We were unable to detect expression of Ffar3 mRNA at any stage of differentiation. Consistent with a lack of Ffar2 expression in the first 24 h of differentiation, acetate and propionate had no significant effect on nuclear receptor expression. Furthermore, acetate or propionate treatment did not alter leptin concentration or triglyceride content. In conclusion, we observed no significant effect of propionate or acetate on adipogenesis in 3T3-L1 cells using validated quantitative techniques

Novel associations of bile acid diarrhoea with fatty liver disease and gallstones: a cohort retrospective analysis.

Background Bile acid diarrhoea (BAD) is a common cause of chronic diarrhoea with a population prevalence of primary BAD around 1%. Previous studies have identified associations with low levels of the ileal hormone fibroblast growth factor 19 (FGF19), obesity and hypertriglyceridaemia. The aim of this study was to identify further associations of BAD. Methods A cohort of patients with chronic diarrhoea who underwent 75selenohomocholic acid taurate (SeHCAT) testing for BAD was further analysed retrospectively. Additional clinical details available from the electronic patient record, including imaging, colonoscopy, chemistry and histopathology reports were used to calculate the prevalence of fatty liver disease, gallstones, colonic neoplasia and microscopic colitis, which was compared for BAD, the primary BAD subset and control patients with diarrhoea. Findings Of 578 patients, 303 (52%) had BAD, defined as a SeHCAT 7d retention value 31 ng/mL with imaging showing fatty liver (p40 IU/L. In 176 subjects with gallbladder imaging, 27% had gallstones, 7% had a prior cholecystectomy and 34% either of these. The median SeHCAT values were lower in those with gallstones (3.8%, p<0.0001), or gallstones/cholecystectomy (7.2%, p<0.001), compared with normal gallbladder imaging (14%). Overall, BAD had an OR of 2.0 for gallstones/cholecystectomy (p<0.05). BAD was not significantly associated with colonic adenoma/carcinoma or with microscopic colitis. Interpretation The diagnosis of BAD is associated with fatty liver disease and with gallstones. The reasons for these associations require further investigation into potential metabolic causes

Wolbachia endosymbionts induce neutrophil extracellular trap formation in human onchocerciasis

The endosymbiotic bacteria, Wolbachia, induce neutrophilic responses to the human helminth pathogen Onchocerca volvulus. The formation of Neutrophil Extracellular Traps (NETs), has been implicated in anti-microbial defence, but has not been identified in human helminth infection. Here, we demonstrate NETs formation in human onchocerciasis. Extracellular NETs and neutrophils were visualised around O. volvulus in nodules excised from untreated patients but not in nodules from patients treated with the anti-Wolbachia drug, doxycycline. Whole Wolbachia or microspheres coated with a synthetic Wolbachia lipopeptide (WoLP) of the major nematode Wolbachia TLR2/6 ligand, peptidoglycan associated lipoprotein, induced NETosis in human neutrophils in vitro. TLR6 dependency of Wolbachia and WoLP NETosis was demonstrated using purified neutrophils from TLR6 deficient mice. Thus, we demonstrate for the first time that NETosis occurs during natural human helminth infection and demonstrate a mechanism of NETosis induction via Wolbachia endobacteria and direct ligation of Wolbachia lipoprotein by neutrophil TLR2/6

A Fast and Exact w-stacking and w-projection Hybrid Algorithm for Wide-field Interferometric Imaging

The standard wide-field imaging technique, the w-projection, allows correction for wide fields of view for non-coplanar radio interferometric arrays. However, calculating exact corrections for each measurement has not been possible due to the amount of computation required at high resolution and with the large number of visibilities from current interferometers. The required accuracy and computational cost of these corrections is one of the largest unsolved challenges facing next-generation radio interferometers such as the Square Kilometre Array. We show that the same calculation can be performed with a radially symmetric w-projection kernel, where we use one-dimensional adaptive quadrature to calculate the resulting Hankel transform, decreasing the computation required for kernel generation by several orders of magnitude, while preserving the accuracy. We confirm that the radial w-projection kernel is accurate to approximately 1% by imaging the zero-spacing with an added w-term. We demonstrate the potential of our radially symmetric w-projection kernel via sparse image reconstruction, using the software package PURIFY. We develop a distributed w-stacking and w-projection hybrid algorithm. We apply this algorithm to individually correct for non-coplanar effects in 17.5 million visibilities over a 25 by 25 degree FoV Murchison Widefield Array observation for image reconstruction. Such a level of accuracy and scalability is not possible with standard w-projection kernel generation methods. This demonstrates that we can scale to a large number of measurements with large image sizes while still maintaining both speed and accuracy