Influence of previous crops and nematicide treatments on root lesion nematode populations and crop yields

Nous avons étudié au champ l'influence du précédent cultural, de la séquence culturelle et de traitements à l'aldicarbe sur les populations de nematodes des nodosités et sur les rendements de diverses cultures à l'île-du Prince-Édouard. La culture précédente a eu le plus grand impact sur le nombre de nematodes. Les séquences des cultures ont parfois eu une influence sur les populations de nematodes des nodosités (principalement Pratylenchus penetrans) et sur les rendements. Dans les champs commerciaux d'orge (Hordeum vulgare), les nematodes des nodosités étaient plus nombreux lorsque l'orge suivait une culture combinée de trèfle rouge (Trifolium pratense) et de fléole (Phleum pratense) et les nematodes du rabougrissement (Tylenchorynchus spp., principalement T. dubiuset Merlinius spp.) étaient plus nombreux quand l'orge était semée pendant deux années consécutives. La masse de la matière sèche combinée du feuillage et du grain était plus importante lorsque l'orge était semée auprès des pommes de terre (Solanum tuberosum) et cette masse était plus faible avec deux cultures successives d’orge ou avec un mélange de trèfle rouge et de fléole. En conditions expérimentales au champ, les populations de nématodes des nodosités étaient plus élevées dans les racines d’orge lorsque celle-ci était cultivée après des pommes de terre et les rendements en grain étaient plus faibles lorsque l’orge était cultivée pendant deux années consécutives. Les changements dans les populations de nématodes chez la pomme de terre n’étaient pas associés aux séquences débutant avec le blé (Triticum aestivum) ou l’orge que dans les séquences débutant avec les pommes de terre ou le soya (Glycine max). L’aldicarbe a réduit le nombre de nématodes des nodosités en plus d’accroître les rendements en pommes de terre et en soya.A field study assessing the influence of the previous crop, the crop sequence, and aldicarb treatments on root lesion nematode populations and crop yields was carried out in Prince-Edward-Island, Canada. The most recent crop had the greatest impact on nematode numbers. The crop sequences had an influence in some cases on root lesion nematode populations (primarily Pratylenchus penetrans) and on crop yields. In commercial barley (Hordeum vulgare) fields, root lesion nematodes in roots were greatest when barley followed a red clover (Trifolium pratense) timothy (Phleum pratense) ley, and stunt nematodes (Tylenchorhynchus spp., primarily T. dubius, and Merlinius spp.) were more common when barley followed barley. The combined dry weight of foliage and grain was larger when barley was planted after potato (Solanumtuberosum) and smaller when barley followed barley or a red clover-timothy mixture. Under experimental field conditions, root lesion nematode populations were largest in barley roots when barley followed potato and grain yields were smallest when barley followed barley. Changes in nematode populations in potato were not associated with crop sequences. Potato tuber yields were higher in the sequences that began with wheat (Triticum aestivum) or barley than in the sequences that began with potato or soybean (Glycine max). Aldicarb reduced the numbers of root lesion nematodes in roots with concomitant yield increases in potato and soybean

A new wrinkle on the enhancon

We generalize the basic enhancon solution of Johnson, Peet and Polchinski by constructing solutions without spherical symmetry. A careful consideration of boundary conditions at the enhancon surface indicates that the interior of the supergravity solution is still flat space in the general case. We provide some explicit analytic solutions where the enhancon locus is a prolate or oblate sphere.Comment: 19 pages, no figure

Paradoxical effects of the cannabinoid CB2 receptor agonist GW405833 on rat osteoarthritic knee joint pain

SummaryObjectiveThe present study examined whether local administration of the cannabinoid-2 (CB2) receptor agonist GW405833 could modulate joint nociception in control rat knee joints and in an animal model of osteoarthritis (OA).MethodOA was induced in male Wistar rats by intra-articular injection of sodium monoiodo-acetate with a recovery period of 14 days. Immunohistochemistry was used to evaluate the expression of CB2 and transient receptor potential vanilloid channel-1 (TRPV1) receptors in the dorsal root ganglion (DRG) and synovial membrane of sham- and sodium mono-iodoacetate (MIA)-treated animals. Electrophysiological recordings were made from knee joint primary afferents in response to rotation of the joint both before and following close intra-arterial injection of different doses of GW405833. The effect of intra-articular GW405833 on joint pain perception was determined by hindlimb incapacitance. An in vitro neuronal release assay was used to see if GW405833 caused release of an inflammatory neuropeptide (calcitonin gene-related peptide – CGRP).ResultsCB2 and TRPV1 receptors were co-localized in DRG neurons and synoviocytes in both sham- and MIA-treated animals. Local application of the GW405833 significantly reduced joint afferent firing rate by up to 31% in control knees. In OA knee joints, however, GW405833 had a pronounced sensitising effect on joint mechanoreceptors. Co-administration of GW405833 with the CB2 receptor antagonist AM630 or pre-administration of the TRPV1 ion channel antagonist SB366791 attenuated the sensitising effect of GW405833. In the pain studies, intra-articular injection of GW405833 into OA knees augmented hindlimb incapacitance, but had no effect on pain behaviour in saline-injected control joints. GW405833 evoked increased CGRP release via a TRPV1 channel-dependent mechanism.ConclusionThese data indicate that GW405833 reduces the mechanosensitivity of afferent nerve fibres in control joints but causes nociceptive responses in OA joints. The observed pro-nociceptive effect of GW405833 appears to involve TRPV1 receptors

Theory of Melting and the Optical Properties of Gold/DNA Nanocomposites

We describe a simple model for the melting and optical properties of a DNA/gold nanoparticle aggregate. The optical properties at fixed wavelength change dramatically at the melting transition, which is found to be higher and narrower in temperature for larger particles, and much sharper than that of an isolated DNA link. All these features are in agreement with available experiments. The aggregate is modeled as a cluster of gold nanoparticles on a periodic lattice connected by DNA bonds, and the extinction coefficient is computed using the discrete dipole approximation. Melting takes place as an increasing number of these bonds break with increasing temperature. The melting temperature corresponds approximately to the bond percolation threshold.Comment: 5 pages, 4 figure. To be published in Phys. Rev.

Integrating Multidisciplinary Design in an Undergraduate Curriculum

Multidisciplinary design and analysis has become the normal mode of operation within most aerospace companies, but the impact of these changes have largely not been reflected at many universities. In an effort to determine if the emergence of multidisciplinary design concepts should influence engineering curricula, NASA has asked several universities (Virginia Tech, Georgia Tech, Clemson, BYU, and Cal Poly) to investigate the practicality of introducing the concepts within their undergraduate curricula. A multidisciplinary team of faculty, students, and industry partners evaluated the aeronautical engineering curriculum at Cal Poly. A variety of ways were found to introduce multidisciplinary themes into the existing program. Both analytic and educational tools for multidisciplinary design of aircraft have been developed and are in the process of being implemented

B-1a cells acquire their unique characteristics by bypassing the pre-BCR selection stage

B-1a cells are long-lived, self-renewing innate-like B cells that predominantly inhabit the peritoneal and pleural cavities. In contrast to conventional B-2 cells, B-1a cells have a receptor repertoire that is biased towards bacterial and self-antigens, promoting a rapid response to infection and clearing of apoptotic cells. Although B-1a cells are known to primarily originate from fetal tissues, the mechanisms by which they arise has been a topic of debate for many years. Here we show that in the fetal liver versus bone marrow environment, reduced IL-7R/STAT5 levels promote immunoglobulin kappa gene recombination at the early pro-B cell stage. As a result, differentiating B cells can directly generate a mature B cell receptor (BCR) and bypass the requirement for a pre-BCR and pairing with surrogate light chain. This 'alternate pathway' of development enables the production of B cells with self-reactive, skewed specificity receptors that are peculiar to the B-1a compartment. Together our findings connect seemingly opposing lineage and selection models of B-1a cell development and explain how these cells acquire their unique properties