70 research outputs found

    Homogeneous dielectric heating in large microwave ovens by excitation of multiple eigenmodes at their resonance frequencies

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    [EN] The proper use of microwave heating can significantly increase the production cycle time and energy efficiency in industrial heating processes compared to conventional heating methods. The main challenge of this technique is to improve the temperature uniformity in the product exposed to standing waves inside the microwave oven. In opposite to the magnetron, solid-state amplifiers (SSA) offer the possibility to increase the homogeneity by changing the amplitude, frequency and phase with the help of intelligent control methods [1]. In this work, the variation of the frequency and the amplitude of the SSA is considered. The multimode microwave oven used in the experiment has an industrial size of 535 mm x 510 mm x 395 mm (Figure 1). The SSA was operated in the frequency range from 2.4 GHz to 2.5 GHz. It consisted of a new 300 W solid state microwave source from HBH microwave GmbH, Germany. An antenna system was developed based on numerical simulation with CST Microwave Studio. The positions of four loop antennas were optimized to excite at least 90 % of the possible 32 eigenmodes [2] of the unloaded cavity. At the roof of the cavity, an IR camera was installed to observe the temperature distribution of the load during heating. A sheet of paper was used as the thermal load. It was placed on a PTFE plate as a sample holder. Figure 1 exemplary illustrates the comparison of the simulated power distribution with the measured temperature distribution for two representative eigenmodes. As can be expected from the figures, an optimized combination of different modes will lead to a significantly improved temperature uniformity in the material. Latest results obtained with different type of loads will be presented.The authors acknowledge the financial support by the Federal Ministry for Economic Affairs and Energy of Germany in the project ZF4204602PR6.Neumaier, D.; Sanseverino, S.; Link, G.; Jelonnek, J. (2019). Homogeneous dielectric heating in large microwave ovens by excitation of multiple eigenmodes at their resonance frequencies. En AMPERE 2019. 17th International Conference on Microwave and High Frequency Heating. Editorial Universitat Politècnica de València. 166-173. https://doi.org/10.4995/AMPERE2019.2019.9877OCS16617

    Autonomous Bioluminescent Expression of the Bacterial Luciferase Gene Cassette (lux) in a Mammalian Cell Line

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    The bacterial luciferase (lux) gene cassette consists of five genes (luxCDABE) whose protein products synergistically generate bioluminescent light signals exclusive of supplementary substrate additions or exogenous manipulations. Historically expressible only in prokaryotes, the lux operon was re-synthesized through a process of multi-bicistronic, codon-optimization to demonstrate for the first time self-directed bioluminescence emission in a mammalian HEK293 cell line in vitro and in vivo.Autonomous in vitro light production was shown to be 12-fold greater than the observable background associated with untransfected control cells. The availability of reduced riboflavin phosphate (FMNH(2)) was identified as the limiting bioluminescence substrate in the mammalian cell environment even after the addition of a constitutively expressed flavin reductase gene (frp) from Vibrio harveyi. FMNH(2) supplementation led to a 151-fold increase in bioluminescence in cells expressing mammalian codon-optimized luxCDE and frp genes. When injected subcutaneously into nude mice, in vivo optical imaging permitted near instantaneous light detection that persisted independently for the 60 min length of the assay with negligible background.The speed, longevity, and self-sufficiency of lux expression in the mammalian cellular environment provides a viable and powerful alternative for real-time target visualization not currently offered by existing bioluminescent and fluorescent imaging technologies

    Biotechnology in the sustainable environment

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    Saccharomyces cerevisiae BLYAS, a New Bioluminescent Bioreporter for Detection of Androgenic Compoundsâ–¿

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    A Saccharomyces cerevisiae strain, capable of autonomous bioluminescence, was engineered to respond to androgenic chemicals. The strain, S. cerevisiae BLYAS, contains the human androgen receptor in the chromosome and was constructed by inserting a series of androgen response elements between divergent yeast promoters GPD and ADH1 on pUTK401 that constitutively expressed luxA and luxB to create pUTK420. Cotransformation of this plasmid with a second plasmid (pUTK404), containing the genes required for aldehyde synthesis (luxCDE) and FMN reduction (frp), yielded a bioluminescent bioreporter responsive to androgenic chemicals. Using dihydrotestosterone (DHT) as a standard, the response time and the 50% effective concentration values were 3 to 4 h and (9.7 ± 4.6) × 10−9 M, respectively. The lower limit of detection in response to DHT was 2.5 × 10−9 M, and in response to testosterone it was 2.5 × 10−10 M. This strain is suitable for high-throughput screening of chemicals with potential for remote environmental monitoring systems because of the assay speed, sensitivity, and self-containment

    A pilot study of prehospital antibiotics for severe sepsis

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    The primary aim of our investigation was to describe the safety and feasibility of a protocol for prehospital recognition of sepsis with hypotension and septic shock, drawing of blood cultures, and administration of intravenous (IV) antibiotics in an urban EMS service, thereby adding to the limited U.S. literature available on this subject and supporting the development of a large-scale randomized control trial (RCT). Primary feasibility measures included the frequency of allergic reactions, culture contamination, and paramedic adherence to the protocol in the prehospital environment
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