Population Genetics of the blow fly Phormia regina (Meigen)

poster abstractBlow flies (Diptera: Calliphoridae) are a family of flies which are associated with the decomposition of organic matter, and the many species are widely distributed across the globe inhabiting almost every ecosystem and continent, with the exception being Antarctica. For many years, biologists have been interested in these metamorphic insects with much attention in recent years with their predictable life cycle and subsequent application in death investigations. Phormia regina (Meigen) is a primary colonizer of carrion and is widely distributed across the United States and can be found throughout the year. Recently, molecular techniques have been applied to a number of blow fly species, initially for identification purposes to corroborate morphological taxonomy but also as an alternative to morphology when specimens are damaged. Even more recently, population structure of these flies have been investigated, however these studies have primarily focused on pest species which infest living livestock and directly affect the agricultural economy. Here, we build upon previous analysis with AFLP markers on P. regina to determine the population structure of this integral species by using tetra-nucleotide microsatellite markers. From the recently constructed draft genome of P. regina, several thousand tetra-nucleotide (four-base repeating sequences of DNA) were identified. Due to the high A-T content of insect DNA, repeats consisting wholly of adenine and thymine bases were discarded. From the remaining tetra-repeats, 14 were chosen for initial analysis to determine their polymorphic nature; from here six were selected for genotypic analysis. More than 200 different P. regina individuals belonging to 27 populations collected from across the contiguous United States have been genotyped across these six tetra-nucleotide loci. Five loci possess ≥30 different alleles, while the sixth appears to be fairly conserved with eight different alleles. Heterozygosity exhibited between these populations is uniform (0.5-0.8) while inbred lab colonies exhibit an increase in homozygosity from their ancestral population. Further analyses are to be performed in order to determine the population structure and geography-genetic relationships of these flies

Consumer\u27s choice in protective eyewear

Seventeen pairs of racquetball eyewear were tested for distortion and loss of field. Four of the seventeen eyewear produced twenty five percent or greater loss of field. Two of the eyewears produced less than one percent loss of field. None produced measurable distortion utilized by our measurement techniques

The Mink Frog, Rana septentrionalis, in Southeastern Labrador

Mink Frogs (Rana septentrionalis) were collected at three new localities in southeastern Labrador in 2003 and 2005, extending the known range of the species about 200 km to the east, to the limits of the coast.La Grenouille du Nord (Rana septentrionalis) a été trouvée dans trois nouvelles localités dans le sud-est du Labrador en 2003 et 2005. Ces mentions élargissent l’aire de répartition connue de l’espèce d’environ 200 km vers l’est, aux limites de la côte de l’Océan Atlantique

Effect of Interfacial Tension on Propagating Polymerization Fronts

This paper is devoted to the investigation of polymerization fronts converting a liquid monomer into a liquid polymer. We assume that the monomer and the polymer are immiscible and study the influence of the interfacial tension on the front stability. The mathematical model consists of the reaction-diffusion equations coupled with the Navier-Stokes equations through the convection terms. The jump conditions at the interface take into account the interfacial tension. Simple physical arguments show that the same temperature distribution could not lead to Marangoni instability for a nonreacting system. We fulfill a linear stability analysis and show that interaction of the chemical reaction and of the interfacial tension can lead to an instability that has another mechanism: the heat produced by the reaction decreases the interfacial tension and initiates the liquid motion. It brings more monomer to the reaction zone and increases even more the heat production. This feedback mechanism can lead to the instability if the frontal Marangoni number exceeds a critical value. (C) 2000 American Institute of Physics. [S1054-1500(00)01701-8]

Optimization of total vaporization solid-phase microextraction (TV-SPME) for the determination of lipid profiles of Phormia regina, a forensically important blow fly species

A new method has been developed for the determination of fatty acids, sterols, and other lipids which naturally occur within pupae of the blow fly Phormia regina. The method relies upon liquid extraction in non-polar solvent, followed by derivatization using N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) w/ 1% trimethylchlorsilane (TMCS) carried out inside the sample vial. The analysis is facilitated by total vaporization solid-phase microextraction (TV-SPME), with gas chromatography-mass spectrometry (GC-MS) serving as the instrumentation for analysis. The TV-SPME delivery technique is approximately a factor of five more sensitive than traditional liquid injection, which may alleviate the need for rotary evaporation, reconstitution, collection of high performance liquid chromatography fractions, and many of the other pre-concentration steps that are commonplace in the current literature. Furthermore, the ability to derivatize the liquid extract in a single easy step while increasing sensitivity represents an improvement over current derivatization methods. The most common lipids identified in fly pupae were various saturated and unsaturated fatty acids ranging from lauric acid (12:0) to arachinoic acid (20:4), as well as cholesterol. The concentrations of myristic acid (14:0), palmitelaidic acid (16:2), and palmitoleic acid (16:1) were the most reliable indicators of the age of the pupae

Detecting Awareness in the Vegetative State

peer reviewe

Shaping the corporate perimeter in a changing media industry

A constant theme in strategic media management literature is the transformational impact that digital media technologies and deregulation have had on shaping media firms’ corporate strategies. Whilst the role of corporate strategy is to encapsulate a firm’s long-term direction and scope of activities, it will also give a strong indication of how the firm will compete and be positioned in an industry. However, the transformative effects of a highly technological media environment have changed our traditional view of how the media industry is defined, and so developing a strategic recipe for competing in an ill-defined industry becomes more challenging. This paper examines a single media firm’s corporate strategy and perimeter and considers this in the context of a changing media industry. The paper takes a practice-led approach by undertaking a longitudinal analysis of a firm’s acquisition and divestment activities in order to understand its corporate perimeter and by implication the industry or industries where it competes. We argue that by exploring a media firm’s corporate strategy and perimeter over time, scholars will not only be able to better understand the dynamics of media practice and strategy, but also gain an insight into the changing nature of the media industry. The paper concludes that Porter’s (1980) seminal work on industry structure, profitability and attractiveness remains a relevant form of strategic analysis that can help media management researchers to conceptualize and understand the evolution of media firm corporate perimeter and the industries in which they compete

Factors Affecting Species Identifications of Blow Fly Pupae Based upon Chemical Profiles and Multivariate Statistics

Alternative methods for the identification of species of blow fly pupae have been developed over the years that consist of the analyses of chemical profiles. However, the effect of biotic and abiotic factors that could influence the predictive manner for the tests have not been evaluated. The lipids of blowfly pupae (Cochliomyia macellaria, Lucilia cuprina, Lucilia sericata, and Phormia regina) were extracted in pentane, derivatized, and analyzed by total-vaporization solid phase microextraction gas chromatography-mass spectrometry (TV-SPME GC-MS). Peak areas for 26 compounds were analyzed. Here we evaluated one biotic factor (colonization) on four species of blow flies to determine how well a model produced from lipid profiles of colonized flies predicted the species of flies of offspring of wild-caught flies and found very good species identification following 10 generations of inbreeding. When we evaluated four abiotic factors in our fly rearing protocols (temperature, humidity, pupation substrate, and diet), we found that the ability to assign the chemical profile to the correct species was greatly reduced