Pressing the Nuts to Sheet Metal Blanks

The bachelor thesis consists of the theoretical introduction into pressing the nuts, familiarization with used machines followed by testing and evaluating of outcomes. Pressing the nuts is tested for torque and pulling force. In the practical part of the thesis chosen press nuts will be tested. Objective of this thesis is to find out suitable pressing parameters, which can substitute present unsuitable practices

Additional file 3: of Ixodes scapularis and Ixodes ricinus tick cell lines respond to infection with tick-borne encephalitis virus: transcriptomic and proteomic analysis

TBEV infection levels in mock-infected and TBEV-infected tick cells. Numbers of copies of TBEV NS5 were determined by qRT-PCR using NS5 primers and the linearised plasmid pJET-NS5 to create a standard curve. Copy numbers were normalised to 1 μg of total RNA. The limit of detection was derived from the number of NS5 copies in the highest dilution which was still detectable with a variance less than one Ct and was normalised to 1 μg of total RNA. (A) IDE8 infected and mock-infected (control) at days 2 (2d) and 6 (6d) p.i. (B) IRE/CTVM19 infected and mock-infected (control) at days 2 and 6 p.i.. Error bars are standard deviations. Samples marked with + passed both RNA and protein quality checks and were used in transcriptomic and proteomic analyses