197 research outputs found
Towards many colors in FISH on 3D-preserved interphase nuclei
The article reviews the existing methods of multicolor FISH on nuclear targets, first of all, interphase chromosomes. FISH proper and image acquisition are considered as two related components of a single process. We discuss (1) M-FISH (combinatorial labeling + deconvolution + widefield microscopy); (2) multicolor labeling + SIM (structured illumination microscopy); (3) the standard approach to multicolor FISH + CLSM (confocal laser scanning microscopy; one fluorochrome - one color channel); (4) combinatorial labeling + CLSM; (5) non-combinatorial labeling + CLSM + linear unmixing. Two related issues, deconvolution of images acquired with CLSM and correction of data for chromatic Z-shift, are also discussed. All methods are illustrated with practical examples. Finally, several rules of thumb helping to choose an optimal labeling + microscopy combination for the planned experiment are suggested. Copyright (c) 2006 S. Karger AG, Basel
Basic Atomic Physics
Contains reports on five research projects.National Science Foundation Grant PHY 89-19381U.S. Navy - Office of Naval Research Grant N00014-90-J-1322Joint Services Electronics Program Contract DAAL03-89-C-0001National Science Foundation Grant PHY 86-05893U.S. Army Research Office Contract DAAL03-89-K-0082U.S. Navy - Office of Naval Research Grant N00014-89-J-1207U.S. Navy - Office of Naval Research Grant N00014-90-J-164
Personalized Nutrition: Translating the Science of NutriGenomics Into Practice: Proceedings From the 2018 American College of Nutrition Meeting
Adverse reactions to foods and adverse drug reactions are inherent in product defects, medication errors, and differences in individual drug exposure. Pharmacogenetics is the study of genetic causes of individual variations in drug response and pharmacogenomics more broadly involves genome-wide analysis of the genetic determinants of drug efficacy and toxicity. The similarity of nutritional genomics and pharmacogenomics stems from the innate goal to identify genetic variants associated with metabolism and disease. Thus, nutrigenomics can be thought of as encompassing gene–diet interactions involving diverse compounds that are present in even the simplest foods. The advances in the knowledge base of the complex interactions among genotype, diet, lifestyle, and environment is the cornerstone that continues to elicit changes in current medical practice to ultimately yield personalized nutrition recommendations for health and risk assessment. This information could be used to understand how foods and dietary supplements uniquely affect the health of individuals and, hence, wellness. The individual’s gut microbiota is not only paramount but pivotal in embracing the multiple-functional relationships with complex metabolic mechanisms involved in maintaining cellular homeostasis. The genetic revolution has ushered in an exciting era, one in which many new opportunities are expected for nutrition professionals with expertise in nutritional genomics. The American College of Nutrition’s conference focused on “Personalized Nutrition: Translating the Science of NutriGenomics Into Practice” was designed to help to provide the education needed for the professional engagement of providers in the personalized medicine era.https://doi.org/10.1080/07315724.2019.158298
Basic Atomic Physics
Contains reports on five research projects.National Science Foundation Grant PHY 89-19381National Science Foundation Grant PHY 92-21489U.S. Navy - Office of Naval Research Grant N00014-90-J-1322Joint Services Electronics Program Contract DAAL03-92-C-0001National Science Foundation Grant PHY 89-21769U.S. Army - Office of Scientific Research Grant DAAL03-92-G-0229U.S. Navy - Office of Naval Research Grant N00014-89-J-1207U.S. Navy - Office of Naval Research Grant N00014-90-J-164
Evidence of and search for double-charmonium production in and decays
Using data samples of and
events collected with the Belle detector, a first experimental
search has been made for double-charmonium production in the exclusive decays
, where , , , , and . No significant signal is
observed in the spectra of the mass recoiling against the reconstructed
or except for the evidence of production with a
significance of for . The
measured branching fraction \BR(\Upsilon(1S)\rightarrow J/\psi+\chi_{c1}) is
. The
confidence level upper limits on the branching fractions of the other modes
having a significance of less than are determined. These results are
consistent with theoretical calculations using the nonrelativistic QCD
factorization approach.Comment: 12 pages, 4 figures, 1 table. The fit range was extended to include
X(4160) signal according to referee's suggestions. Other results unchanged.
Paper was accepted for publication as a regular article in Physical Review
Measurement of the Atmospheric Muon Charge Ratio at TeV Energies with MINOS
The 5.4 kton MINOS far detector has been taking charge-separated cosmic ray
muon data since the beginning of August, 2003 at a depth of 2070
meters-water-equivalent in the Soudan Underground Laboratory, Minnesota, USA.
The data with both forward and reversed magnetic field running configurations
were combined to minimize systematic errors in the determination of the
underground muon charge ratio. When averaged, two independent analyses find the
charge ratio underground to be 1.374 +/- 0.004 (stat.) +0.012 -0.010(sys.).
Using the map of the Soudan rock overburden, the muon momenta as measured
underground were projected to the corresponding values at the surface in the
energy range 1-7 TeV. Within this range of energies at the surface, the MINOS
data are consistent with the charge ratio being energy independent at the two
standard deviation level. When the MINOS results are compared with measurements
at lower energies, a clear rise in the charge ratio in the energy range 0.3 --
1.0 TeV is apparent. A qualitative model shows that the rise is consistent with
an increasing contribution of kaon decays to the muon charge ratio.Comment: 16 pages, 17 figure
Measurement of neutrino velocity with the MINOS detectors and NuMI neutrino beam
The velocity of a ~3 GeV neutrino beam is measured by comparing detection times at the near and far detectors of the MINOS experiment, separated by 734 km. A total of 473 far detector neutrino events was used to measure (v-c)/c=5.12.910-5 (at 68% C.L.). By correlating the measured energies of 258 charged-current neutrino events to their arrival times at the far detector, a limit is imposed on the neutrino mass of mnu<50 MeV/c2 (99% C.L.)
Observation of and search for violation in radiative charm decays
We report the first observation of the radiative charm decay and the first search for violation in decays , , and , using a data sample of
943 fb collected with the Belle detector at the KEKB asymmetric-energy
collider. The branching fraction is measured to be , where the first
uncertainty is statistical and the second is systematic. The obtained
asymmetries, , , and
, are consistent with no violation. We also present an improved
measurement of the branching fractions and
Observation of an alternative candidate in
We perform a full amplitude analysis of the process , where refers to either or . A new
charmoniumlike state that decays to is observed with a
significance of . Its mass is ()
MeV/ and width is () MeV. The
hypothesis is favored over the hypothesis at the level
of . The analysis is based on the 980 data sample
collected by the Belle detector at the asymmetric-energy collider
KEKB.Comment: 17 pages, 11 figure
Basic Atomic Physics
Contains reports on five research projects.Joint Services Electronics Program Contract DAAL03-89-C-0001National Science Foundation Grant PHY 87-06560National Science Foundation Contract PHY 86-05893U.S. Army Research Office Contract DAAL03-89-K-0082U.S. Navy - Office of Naval Research Contract N00014-89-J-1207U.S. Navy - Office of Naval Research Contract N00014-83-K-069
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