Emoções no desporto: importância da sua identificação, compreensão e gestão no futebol de formação

It is visible to anyone participating or just watching sports competitions that emotional factors of athletes can change their performance, as in a sense of reduction, or in a sense of increase. Through this study, the main goal is to balance a view of the emotions experienced by young football players, through a logic of understanding of emotional influence in promoting or loss of athletic performance. The study lies within 16 athletes from two youth football teams, in Youth league (born between 1999-2000) and Juniors league (born between 1997-1998), the Academic Football Martim Club, affiliated with the Football Association of Braga, competing in the District Championship of Braga, during the period 2015/2016. The study had led to identifying emotions felt with greater intensity in pre and post sports competition contexts; realize the facilitator character or debilitating emotions in sports performance, as well as comparisons between the two group leagues.info:eu-repo/semantics/publishedVersio

Spectrofluorimetric determination of iodine in urine samples with on-line UV photooxidation using a miniaturized analyzer chip in a multi-syringe flow system

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An integrated in vitro approach unveils the biocompetence and glutathiolomic profile of a human hepatocyte-like cell 3d model

Funding: This work was supported by FCT (Portugal) through the research grant PTDC/MED-TOX/29183/2017. Acknowledgments: The authors thank ECBio S.A. for providing the hnMSCs and F.A. Beland (NCTR, Jefferson, AR, USA) for the kind donation of nevirapine. FCT (UID/DTP/04138/2019, UID/QUI/00100/2019, RECI/QEQ-MED/0330/2012, SFRH/BD/144130/2019 to J.S.R., SFRH/BD/110945/2015 to P.F.P. and CEECIND/02001/2017 to A.M.M.A) are also acknowledged.The need for competent in vitro liver models for toxicological assessment persists. The differentiation of stem cells into hepatocyte-like cells (HLC) has been adopted due to its human origin and availability. Our aim was to study the usefulness of an in vitro 3D model of mesenchymal stem cell-derived HLCs. 3D spheroids (3D-HLC) or monolayer (2D-HLC) cultures of HLCs were treated with the hepatotoxic drug nevirapine (NVP) for 3 and 10 days followed by analyses of Phase I and II metabolites, biotransformation enzymes and drug transporters involved in NVP disposition. To ascertain the toxic effects of NVP and its major metabolites, the changes in the glutathione net flux were also investigated. Phase I enzymes were induced in both systems yielding all known correspondent NVP metabolites. However, 3D-HLCs showed higher biocompetence in producing Phase II NVP metabolites and upregulating Phase II enzymes and MRP7. Accordingly, NVP-exposure led to decreased glutathione availability and alterations in the intracellular dynamics disfavoring free reduced glutathione and glutathionylated protein pools. Overall, these results demonstrate the adequacy of the 3D-HLC model for studying the bioactivation/metabolism of NVP representing a further step to unveil toxicity mechanisms associated with glutathione net flux changes.publishersversionpublishe

Study of 3,4-hydroxypyridinones functionalized beads for iron (III) determination in a microsequential injection solid phase spectrophotometry mode

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Chip-based spectrofluorimetric determination of iodine in a multi-syringe flow platform with and without in-line digestion — application to salt, pharmaceuticals, and algae samples

In this work, a flow-based spectrofluorimetric method for iodine determination was developed. The system consisted of a miniaturized chip-based flow manifold for solutions handling and with integrated spectrofluorimetric detection. A multi-syringe module was used as a liquid driver. Iodide was quantified from its catalytic effect on the redox reaction between Ce(IV) and As(III), based on the Sandell–Kolthoff reaction. The method was applied for the determination of iodine in salt, pharmaceuticals, supplement pills, and seaweed samples without off-line pre-treatment. An in-line oxidation process, aided by UV radiation, was implemented to analyse some samples (supplement pills and seaweed samples) to eliminate interferences and release iodine from organo-iodine compounds. This feature, combined with the fluorometric reaction, makes this method simpler, faster, and more sensitive than the classic approach of the Sandell–Kolthoff reaction. The method allowed iodine to be determined within a range of 0.20–4.0 µmol L−1, with or without the in-line UV digestion, with a limit of detection of 0.028 µmol L−1 and 0.025 µmol L−1, respectively.info:eu-repo/semantics/publishedVersio

A miniaturized chip in a multi-syringe flow system for spectrofluorimetric determination of iodide in urine samples

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A robust flow-based system for the spectrophotometric determination of Cr(VI) in recreational waters

A flow-based method for the spectrophotometric determination of chromium (VI) in recreational waters with different salinities was developed. Chromium can occur in the environment in different oxidation states with different related physiological properties. With regard to chromium, the speciation is particularly important, as the hexavalent chromium is considered to be carcinogenic. To achieve that purpose, the use of the diphenylcarbazide (DPC) selective colored reaction with the hexavalent chromium was the chosen strategy. The main objective was to develop a direct and simple spectrophotometric method that could cope with the analysis of different types of environmental waters, within different salinity ranges (fresh to marine waters). The potential interference of metal ions, that can usually be present in environmental waters, was assessed and no significant interferences were observed (<10%). For a complete Cr(VI) determination (three replicas) cycle, the corresponding reagents consumption was 75 µg of DPC, 9 mg of ethanol and 54 mg of sulfuric acid. Each cycle takes about 5 min, including the system clean-up. The limit of detection was 6.9 and 12.2 µg L−1 for waters with low and high salt content, respectively. The method was applied for the quantification of chromium (VI) in both fresh and marine water, and the results were in agreement with the reference procedure.info:eu-repo/semantics/publishedVersio

O enfoque qualitativo na avaliação de programas psicoeducativos: Aplicações e contributos da metodologia qualitativa

Os programas psicoeducativos surgem, nas mais diversas áreas, como uma oportunidade de desenvolvimento pessoal, verificando-se uma demanda crescente de propostas baseadas na evidência. Na avaliação destes programas assiste-se porém a alguma insatisfação com a metodologia quantitativa, sobretudo, quando não permite perceber profundamente os processos de mudança. O objetivo deste trabalho é ilustrar o contributo da metodologia qualitativa à investigação desenvolvida, através da apresentação de três processos distintos: a) entrevista semiestruturada (construída a partir da abordagem fenomenológica e utilizada como avaliação pré-teste, pós-teste e follow-up num programa de educação parental); b) metodologia observacional (útil para avaliação formativa de programas e análise do processo de mudança de comportamentos ao longo dos programas); e c) Q-set da Resiliência Familiar (método misto, originalmente construído para a avaliação do funcionamento resiliente das famílias em risco psicossocial). Apresentam-se os resultados alcançados em estudos realizados e discute-se a pertinência da sua aplicação no âmbito da avaliação da intervenção psicoeducativa.Psychoeducational programmes appear, in several areas, as an opportunity for personal development, and there is a growing demand for evidence-based interventions. Yet, in its evaluation framework has been recognized some dissatisfaction with the quantitative methodology, especially when it does not contribute to deeply understand the processes of change. The objective of this paper is to illustrate the contribution of qualitative methodology to research, presenting three different processes: a) semi-structured interview (it was built considering the phenomenological approach and was used for a pre-test, post-test and follow-up assessment in a parenting educational programme); observational method (useful for training programmes assessment and analysis of the process of behavior change occurred over the programmes); and c) Q-set of family resilience (mixed method, originally built for the evaluation of the resilient functioning of psychosocial risk families). We present the results obtained and we discusses the relevance of its application in the assessment of psychoeducational intervention.info:eu-repo/semantics/publishedVersio

EVIDENCE-BASED PSYCHOEDUCATIONAL INTERVENTION: THE CONTRIBUTION OF QUALITATIVE APPROACHES

Neste trabalho pretende-se discutir o contributo da metodologia qualitativa na avaliação da eficácia dos processos de mudança quando se implementam programas psicoeducativos. Concretamente apresentam-se três recursos distintos: a) entrevista semiestruturada (construída a partir da abordagem fenomenológica e utilizada como avaliação pré-teste, pós-teste e follow-up num programa de educação parental); b) metodologia observacional (útil para avaliação formativa de programas e análise do processo de mudança de comportamentos ao longo dos programas); e c) Q-set da Resiliência Familiar (método misto, originalmente construído para a avaliação do funcionamento resiliente das famílias em risco psicossocial). Cada um dos recursos é discutido, ilustrando-se a sua utilização e a pertinência da sua aplicação no âmbito da avaliação da eficácia da intervenção psicoeducativa.The main goal of this paper is to discuss the contribution of qualitative methodology for the assessment of the changing processes due to psychoeducational intervention. Three different resources are presented: a) semi-structured interview (it was built considering the phenomenological approach and was used for a pre-test, post-test and follow-up assessment in a parenting educational programme); observational method (useful for training programmes assessment and analysis of the process of behavior change occurred over the programmes); and c) Q-set of family resilience (mixed method, originally built for the evaluation of the resilient functioning of psychosocial risk families). These resources are discussed and is stated the relevance of their aplication in the assessment of psychoeducational intervention.info:eu-repo/semantics/publishedVersio

Human-umbilical cord matrix mesenchymal cells improved left ventricular contractility independently of infarct size in swine myocardial infarction with reperfusion

Funding Information: This work was funded by: i) national funds through FCT - Portuguese Foundation for Science and Technology, under the scope of the Cardiovascular R&D Center - UnIC (UIDB/00051/2020 and UIDP/00051/2020); ii) “la Caixa” Banking Foundation and FCT under the project code LCF/PR/HP17/52190002”; iii) the QREN project 2013/30196; and iv) the European Structural and Investment Funds (ESIF), under the Lisbon Portugal Regional Operational Program and National Funds through FCT [POCI-01-0145-FEDER-030985]. RNG and TLL were funded by the FCT individual fellowships [SFRH/BD/144490/2019] and [PD/BD/127997/2016], respectively. Funding sources had no interference in the design of the study, study governance, data collection and analysis, nor in manuscript writing or its scientific and intellectual content. Publisher Copyright: 2023 Raposo, Cerqueira, Leite, Moreira-Costa, Laundos, Miranda, Mendes-Ferreira, Coelho, Gomes, Pinto-do-Ó, Nascimento, Lourenço, Cardim and Leite-Moreira.Background: Human umbilical cord matrix-mesenchymal stromal cells (hUCM-MSC) have demonstrated beneficial effects in experimental acute myocardial infarction (AMI). Reperfusion injury hampers myocardial recovery in a clinical setting and its management is an unmet need. We investigated the efficacy of intracoronary (IC) delivery of xenogeneic hUCM-MSC as reperfusion-adjuvant therapy in a translational model of AMI in swine. Methods: In a placebo-controlled trial, pot-belied pigs were randomly assigned to a sham-control group (vehicle-injection; n = 8), AMI + vehicle (n = 12) or AMI + IC-injection (n = 11) of 5 × 105 hUCM-MSC/Kg, within 30 min of reperfusion. AMI was created percutaneously by balloon occlusion of the mid-LAD. Left-ventricular function was blindly evaluated at 8-weeks by invasive pressure-volume loop analysis (primary endpoint). Mechanistic readouts included histology, strength-length relationship in skinned cardiomyocytes and gene expression analysis by RNA-sequencing. Results: As compared to vehicle, hUCM-MSC enhanced systolic function as shown by higher ejection fraction (65 ± 6% vs. 43 ± 4%; p = 0.0048), cardiac index (4.1 ± 0.4 vs. 3.1 ± 0.2 L/min/m2; p = 0.0378), preload recruitable stroke work (75 ± 13 vs. 36 ± 4 mmHg; p = 0.0256) and end-systolic elastance (2.8 ± 0.7 vs. 2.1 ± 0.4 mmHg*m2/ml; p = 0.0663). Infarct size was non-significantly lower in cell-treated animals (13.7 ± 2.2% vs. 15.9 ± 2.7%; Δ = −2.2%; p = 0.23), as was interstitial fibrosis and cardiomyocyte hypertrophy in the remote myocardium. Sarcomere active tension improved, and genes related to extracellular matrix remodelling (including MMP9, TIMP1 and PAI1), collagen fibril organization and glycosaminoglycan biosynthesis were downregulated in animals treated with hUCM-MSC. Conclusion: Intracoronary transfer of xenogeneic hUCM-MSC shortly after reperfusion improved left-ventricular systolic function, which could not be explained by the observed extent of infarct size reduction alone. Combined contributions of favourable modification of myocardial interstitial fibrosis, matrix remodelling and enhanced cardiomyocyte contractility in the remote myocardium may provide mechanistic insight for the biological effect.publishersversionpublishe