Transmission of Fusarium boothii Mycovirus via Protoplast Fusion Causes Hypovirulence in Other Phytopathogenic Fungi

There is increasing concern regarding the use of fungicides to control plant diseases, whereby interest has increased in the biological control of phytopathogenic fungi by the application of hypovirulent mycoviruses as a possible alternative to fungicides. Transmission of hypovirulence-associated double-stranded RNA (dsRNA) viruses between mycelia, however, is prevented by the vegetative incompatibility barrier that often exists between different species or strains of filamentous fungi. We determined whether protoplast fusion could be used to transmit FgV1-DK21 virus, which is associated with hypovirulence on F. boothii (formerly F. graminearum strain DK21), to F. graminearum, F. asiaticum, F. oxysporum f. sp. lycopersici, and Cryphonectria parasitica. Relative to virus-free strains, the FgV1-DK21 recipient strains had reduced growth rates, altered pigmentation, and reduced virulence. These results indicate that protoplast fusion can be used to introduce FgV1-DK21 dsRNA into other Fusarium species and into C. parasitica and that FgV1-DK21 can be used as a hypovirulence factor and thus as a biological control agent

Identification of a new strain of Ligustrum virus A causing leaf necrosis and chlorosis symptoms in Syringa oblata var. delatata (Nakai) Rehder

In 2020, lilac trees showing virus-like symptoms such as leaf necrosis and chlorosis were observed in Korea. After RT-PCR detection with specific primer sets designed based on previously reported nucleotide sequences of viruses in lilac, the agent was identified as ligustrum virus A (LVA). The complete genome of the virus was sequenced and used for phylogenetic analysis. The genome of this novel strain of LVA, LVA-SNU, is 8524 nucleotides long, excluding the poly(A) tail, and shares the highest nucleotide sequence identity (77.28%) with LVA-Sob, which was detected in a plant of the same species, Syringa oblata, in China, whereas LVA-Sob shares higher sequence identity (97.89%) with LVA-SK, which has been detected in host plants of various species.N

Korejiešu valoda latviešiem, 1.daļa: 46.audiodatne

Korejiešu valodas mācību grāmatas 1. daļas audiodatne 46Darbs ir izstrādāts ar Korejas Republikas Izglītības ministrijas Korejiešu studiju pamatprogrammas un Korejas Studiju akadēmijas Korejas Studiju veicināšanas dienesta (AKS-2015-INC-2230001) atbalstu. The work was supported by Seed program for Korean Studies through the Ministry of Education of Republic of Korea and Korean Studies Promotion Service of the Academy of Korean Studies (AKS 2015-INC-2230001)

Host genes involved in interactions between mycoviruses and host fungi.

<p>Host genes involved in interactions between mycoviruses and host fungi.</p

Highly Specific Detection of Five Exotic Quarantine Plant Viruses using RT-PCR

To detect five plant viruses (Beet black scorch virus, Beet necrotic yellow vein virus, Eggplant mottled dwarf virus, Pelargonium zonate spot virus, and Rice yellow mottle virus) for quarantine purposes, we designed 15 RT-PCR primer sets. Primer design was based on the nucleotide sequence of the coat protein gene, which is highly conserved within species. All but one primer set successfully amplified the targets, and gradient PCRs indicated that the optimal temperature for the 14 useful primer sets was 51.9°C. Some primer sets worked well regardless of annealing temperature while others required a very specific annealing temperature. A primer specificity test using plant total RNAs and cDNAs of other plant virus-infected samples demonstrated that the designed primer sets were highly specific and generated reproducible results. The newly developed RT-PCR primer sets would be useful for quarantine inspections aimed at preventing the entry of exotic plant viruses into Korea

Korejiešu valoda latviešiem, 2.daļa: 10.audiodatne

Korejiešu valodas mācību grāmatas 2. daļas audiodatne 10Darbs ir izstrādāts ar Korejas Republikas Izglītības ministrijas Korejiešu studiju pamatprogrammas un Korejas Studiju akadēmijas Korejas Studiju veicināšanas dienesta (AKS-2015-INC-2230001) atbalstu. The work was supported by Seed program for Korean Studies through the Ministry of Education of Republic of Korea and Korean Studies Promotion Service of the Academy of Korean Studies (AKS 2015-INC-2230001)

Korejiešu valoda latviešiem, 1.daļa: 49.audiodatne

Korejiešu valodas mācību grāmatas 1. daļas audiodatne 4

Korejiešu valoda latviešiem, 1.daļa: 35.audiodatne

Korejiešu valodas mācību grāmatas 1. daļas audiodatne 35Darbs ir izstrādāts ar Korejas Republikas Izglītības ministrijas Korejiešu studiju pamatprogrammas un Korejas Studiju akadēmijas Korejas Studiju veicināšanas dienesta (AKS-2015-INC-2230001) atbalstu. The work was supported by Seed program for Korean Studies through the Ministry of Education of Republic of Korea and Korean Studies Promotion Service of the Academy of Korean Studies (AKS 2015-INC-2230001)

Korejiešu valoda latviešiem 2.daļa: 1.audiodatne

Korejiešu valodas mācību grāmatas 2. daļas audiodatne 1Darbs ir izstrādāts ar Korejas Republikas Izglītības ministrijas Korejiešu studiju pamatprogrammas un Korejas Studiju akadēmijas Korejas Studiju veicināšanas dienesta (AKS-2015-INC-2230001) atbalstu. The work was supported by Seed program for Korean Studies through the Ministry of Education of Republic of Korea and Korean Studies Promotion Service of the Academy of Korean Studies (AKS 2015-INC-2230001)