Tourists behavior during their trip: How they use and offer recommendations?

The rise of new technologies has changed the way tourists trust in eWOM to choose a restaurant. There is a growing use of opinion and price comparison websites, where opinions and ratings can be shared with other users. In addition, the spreading of false or paid comments has made this type of webs seek the generation and maintenance of trust. However, there are few studies that analyse how to generate trust in these webs and its effect in the intention of the consumer to participate in WOM behaviours, once the tourist is already in its tourist destination. Therefore, this research analyses the influence of recommendations on the generation of tourists’ trust in the review websites of restaurant industry while they are in the destination. A regression analysis of data from 439 tourists has revealed that the perceived credibility, the quality of the information and the quality of the web affect trust in review websites. This fact encourages the contracting of restaurant services and communication among consumers, both in a traditional way (WOM) and through the review websites (eWOM), while the tourist is in the tourism destination.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Comprehensive Benchmarking and Integration of Tumour Microenvironment Cell Estimation Methods

Various computational approaches have been developed for estimating the relative abundance of different cell types in the tumour microenvironment (TME) using bulk tumour RNA data. However, a comprehensive comparison across diverse data sets that objectively evaluates the performance of these approaches has not been conducted. Here we benchmarked seven widely used tools and gene sets and introduce ConsensusTME, a method that integrates gene sets from all the other methods for relative TME cell estimation of 18 cell types. We collected a comprehensive benchmark dataset consisting of pan-cancer data (DNA-derived purity, leukocyte methylation, and H&E-derived lymphocyte counts) and cell-specific benchmark data sets (peripheral blood cells and tumour tissues). Although none of the methods outperformed others in every benchmark, ConsensusTME ranked top three in all cancer-related benchmarks and was the best performing tool overall. We provide a web resource to interactively explore the benchmark results and an objective evaluation to help researchers select the most robust and accurate method to further investigate the role of the TME in cancer (www.consensusTME.org).A. Jiménez-Sánchez was supported by a doctoral fellowship from the Cancer Research UK Cambridge Institute and the Mexican National Council of Science and Technology (CONACyT). O. Cast and M.L. Miller were supported by the Brown Performance Group, Innovation in Cancer Informatics Discovery Grant (BD523775). M.L. Miller was supported by Cancer Research UK core grant (C14303/A17197) and the Target Ovarian Cancer Translational Project Grant (Cambridge1320 MM18)

Peripheral T-cell lymphoma with a T follicular-helper phenotype: a different entity? Results of the Spanish Real-T study

Peripheral T-cell lymphomaLimfoma perifèric de cèl·lules TLinfoma periférico de células TNodal peripheral T-cell lymphoma (PTCL) with a T follicular helper phenotype (PTCL-TFH) is a new type of PTCL. We aimed to define its clinical characteristics and prognosis compared to PTCL not otherwise specified (PTCL-NOS) and angioimmunoblastic T-cell lymphoma (AITL). This retrospective observational study included 175 patients diagnosed with PTCL between 2008 and 2013 in 13 Spanish sites. Patient diagnosis was centrally reviewed, and patients were reclassified according to the World Health Organization (WHO) 2016 criteria: 21 patients as PTCL-NOS, 55 as AITL and 23 as PTCL-TFH. Median follow-up was 56.07 months (95% CI 38.7–73.4). Progression-free survival (PFS) and overall survival (OS) were significantly higher in patients with PTCL-TFH than in those with PTCL-NOS and AITL (PFS, 24.6 months vs. 4.6 and 7.8 months, respectively, p = 0.002; OS, 52.6 months vs. 10.0 and 19.3 months, respectively, p < 0.001). Histological diagnosis maintained an independent influence on both PFS (hazard ratio [HR] 4.1 vs. PTCL-NOS, p = 0.008; HR 2.6 vs. AITL, p = 0.047) and OS (HR 5.7 vs. PTCL-NOS, p = 0.004; HR 2.6 vs. AITL, p = 0.096), regardless of the International Prognostic Index. These results suggest that PTCL-TFH could have more favourable features and prognosis than the other PTCL subtypes, although larger series are needed to corroborate these findings.This work was supported by Takeda Farmacéutica España, S.A. The authors received no compensation for writing the manuscript

In vitro Evaluation of Phthalimide Derivatives Against Cancer Cell lines

Los cánceres de pulmón, próstata e hígado se encuentran entre los más prevalentes en los hombres. El cáncer de mama, de cuello uterino y de tiroides se encuentran entre los más prevalentes en mujeres (OMS, 2019). El tratamiento del cáncer generalmente incluye quimioterapia y radioterapia; sin embargo, los medicamentos contra el cáncer disponibles tienen una selectividad baja y causan efectos adversos graves, como nefrotoxicidad, neurotoxicidad y mielosupresión (Matsuo et al., 2010). Por tanto, el diseño y desarrollo de compuestos como nuevos agentes anticancerígenos frente a los tipos de cáncer de mayor incidencia son de vital importancia en el campo de la salud. Los derivados de ftalimida son compuestos prometedores para el desarrollo de nuevos agentes anticancerígenos (Li et al., 2011; Grigalius y Petrikaite, 2017; Kamal et al., 2002). Basado en lo anterior, Este trabajo tuvo como objetivo evaluar la actividad antiproliferativa de 43 derivados de ftalimida contra una línea celular de cáncer principal en hombres (HepG2) y dos líneas celulares de cáncer principales en mujeres (HeLa y 4T1). Además, se determinó la citotoxicidad de los compuestos contra una línea celular de fibroblasto murino normal (3T3). Los resultados mostraron que los compuestos C16, E11 y E16 presentaron la mejor actividad antiproliferativa contra las líneas celulares HeLa y 4T1. El compuesto H16 solo disminuyó la proliferación celular en un 32% contra la línea celular HepG2. Los compuestos H5, H16, E2, E16 y C1 no afectaron a la proliferación de la línea celular 3T3. Demostrando que sería importante continuar con el análisis de este tipo de compuestos frente a diferentes cánceres para encontrar nuevos compuestos con mejor actividad que los actualmente disponibles en el mercado

Post-glacial evolution of alpine environments in the western Mediterranean region : The Laguna Seca record

In an effort to understand how alpine environments from the western Mediterranean region responded to climate variations since the last glacial-interglacial transition, a detailed chronological control and sedimentological analysis, supported by magnetic susceptibility, total organic carbon and C/N data, were carried out on the sedimentary record of Laguna Seca (LS). This is a latitudinal and altitudinally (2259 masl) key alpine wetland site located in the easternmost area of the Sierra Nevada, southern Iberian Peninsula, where sediments accumulated during Heinrich Stadial 1, Bolling-Allerod (B-A) and the Younger Dryas (YD) previously unrecorded in alpine Sierra Nevada. Climate controlled sedimentation in LS and three coarse-grained and one fine-grained facies association are differentiated, which help us decipher the paleoenvironmental evolution of LS: (1) subaerial cohesionless debris flows during a paraglacial stage; (2) till or nival diamicton during a small glacier/nivation hollow stage; (3) massive mudstone by suspension settling of clays into standing water during a lacustrine stage; and (4) frost-shattering breccia deposited inside the lacustrine stage, probably during the YD, and linked to a periglacial substage. The development of a previously existing small glacial cirque during the Last Glacial Maximum (LGM) in the LS basin at an elevation between 2500 and 2300 m could be supported by the important availability of slope sediments glacially-conditioned such as debris flows, reworked by paraglacial slope processes during the first deglaciation stages, confirming previous studies of landforms in the catchment area and the LGM-Equilibrium Line Altitude estimation above 2400 masl in Sierra Nevada. Mean sediment accumulation rates in the LS sedimentary units (4.21 and 0.28 mm/yr during the paraglacial small glacier/nivation stage and the lacustrine stage, respectively) confirm that geomorphic activity accelerated just after glaciers retreated due to a slope adjustment and high availability of glacially conditioned sediments. An abrupt change in paleoenvironmental and paleoclimatic conditions occurred in LS at ~ 15.7 cal kyr BP. This change was probably due to an increase in temperature and precipitation in the western Mediterranean region during the B-A. At LS, this resulted in significant ice-melt, forming a deep-water lake in LS with important organic matter contribution until the end of the Early Holocene (except in the YD when the lake level probably dropped), but elsewhere a general glacier recession in the Sierra Nevada and an expansion of the Mediterranean forest in the southern Iberian Peninsula. Finally, the general long-term aridification that occurred during the Middle Holocene until the present in the western Mediterranean region triggered an important environmental change transforming LS into an ephemeral wetland with an increase in aquatic productivity.Peer reviewe

Swimming performance of <i>Bradyrhizobium diazoefficiens</i> is an emergent property of its two flagellar systems

Many bacterial species use flagella for self-propulsion in aqueous media. In the soil, which is a complex and structured environment, water is found in microscopic channels where viscosity and water potential depend on the composition of the soil solution and the degree of soil water saturation. Therefore, the motility of soil bacteria might have special requirements. An important soil bacterial genus is Bradyrhizobium, with species that possess one flagellar system and others with two different flagellar systems. Among the latter is B. diazoefficiens, which may express its subpolar and lateral flagella simultaneously in liquid medium, although its swimming behaviour was not described yet. These two flagellar systems were observed here as functionally integrated in a swimming performance that emerged as an epistatic interaction between those appendages. In addition, each flagellum seemed engaged in a particular task that might be required for swimming oriented toward chemoattractants near the soil inner surfaces at viscosities that may occur after the loss of soil gravitational water. Because the possession of two flagellar systems is not general in Bradyrhizobium or in related genera that coexist in the same environment, there may be an adaptive tradeoff between energetic costs and ecological benefits among these different species.Facultad de Ciencias ExactasInstituto de Biotecnologia y Biologia MolecularFacultad de IngenieríaInstituto Multidisciplinario de Biología Celula

Swimming performance of <i>Bradyrhizobium diazoefficiens</i> is an emergent property of its two flagellar systems

Many bacterial species use flagella for self-propulsion in aqueous media. In the soil, which is a complex and structured environment, water is found in microscopic channels where viscosity and water potential depend on the composition of the soil solution and the degree of soil water saturation. Therefore, the motility of soil bacteria might have special requirements. An important soil bacterial genus is Bradyrhizobium, with species that possess one flagellar system and others with two different flagellar systems. Among the latter is B. diazoefficiens, which may express its subpolar and lateral flagella simultaneously in liquid medium, although its swimming behaviour was not described yet. These two flagellar systems were observed here as functionally integrated in a swimming performance that emerged as an epistatic interaction between those appendages. In addition, each flagellum seemed engaged in a particular task that might be required for swimming oriented toward chemoattractants near the soil inner surfaces at viscosities that may occur after the loss of soil gravitational water. Because the possession of two flagellar systems is not general in Bradyrhizobium or in related genera that coexist in the same environment, there may be an adaptive tradeoff between energetic costs and ecological benefits among these different species.Facultad de Ciencias ExactasInstituto de Biotecnologia y Biologia MolecularFacultad de IngenieríaInstituto Multidisciplinario de Biología Celula

Appistóteles

El presente trabajo de investigación tiene como finalidad determinar y comprobar la aceptación y viabilidad de la propuesta empresarial llamada Appistóteles. La cual apunta a ofrecer un servicio de asesoría universitaria de calidad a distancia a través de videollamadas por Google Meet. Basado en el entendimiento sobre la coyuntura actual y dificultades en su educación universitaria que el segmento identificado afronta día a día. Por ese motivo, la propuesta ha sido concebida para satisfacer las necesidades de las personas en etapa universitaria con un estilo de vida moderno y progresista, cuya rutina este alineada al uso constante de la tecnología y familiarizada con los canales educativos disponibles actualmente en el mercado. Por ello, Appistóteles se compromete a ofrecer un servicio educativo eficaz e intuitivo, el cual se adaptará a la variedad de cursos disponibles, tipos consultas y disponibilidad de asesores para los alumnos que deseen contar con nuestros servicios. Es preciso señalar que la propuesta será introducida al mercado a través de la redes sociales, avisos digitales y publicidad en vallas, estas últimas ubicadas cerca de las universidades de Lima Metropolitana. Finalmente, y basados en el levantamiento de información de mercado y finanzas proyectados, se determinó que el proyecto es viable debido a que obtuvimos una TIR del 65% y un VAN de S/ 39,571.00 que se encuentran descritos en el desarrollo del presente trabajo.The purpose of this research work is to determine and verify the acceptance and viability of the business called Appistóteles. That aims to offer a quality distance university advising service through video calls on Google Meet. From the knowledge abour the current situation and difficulties in their university education that the identified segment faces every day. Therefore, the proposal has been conceived to satisfy the needs of people in the university stage with a modern and progressive lifestyle, whose routine is aligned with the constant use of technology and familiar with the educational channels currently available on the market. For this reason, Appistóteles is committed to offer an effective and intuitive eduational service, which adapts to the variety of courses available, types of consultations and availability of advisers for students who wish to a have our services. It should be noted that the proposal will be introduced to the market through social networks, digital advertisements and billboards, located near the universities of Lima Metropolitana. Finally, and based on the projected market and financial information obtained, it was determined that the porject is viable because it obtained an IRR of 65% and a VAN of S/ 39,571.00 that are described in the development of this work.Trabajo de investigació

Lymphocyte Profile and Immune Checkpoint Expression in Drug-Induced Liver Injury: An Immunophenotyping Study

The identification of specific HLA risk alleles in drug-induced liver injury (DILI) points toward an important role of the adaptive immune system in DILI development. In this study, we aimed to corroborate the role of an adaptive immune response in DILI through immunophenotyping of leukocyte populations and immune checkpoint expressions. Blood samples were collected from adjudicated DILI (n = 12), acute viral hepatitis (VH; n = 13), acute autoimmune hepatitis (AIH; n = 9), and acute liver injury of unknown etiology (n = 15) at day 1 (recognition), day 7, and day >30. Blood samples from patients with nonalcoholic fatty liver disease (NAFLD; n = 20) and healthy liver controls (HLCs; n = 54) were extracted at one time point. Leukocyte populations and immune checkpoint expressions were determined based on cell surface receptors, except for CTLA-4 that was determined intracellularly, using flow cytometry. At recognition, DILI demonstrated significantly higher levels of activated helper T-cell (P < 0.0001), activated cytotoxic T-cells (P = 0.0003), Th1 (P = 0.0358), intracellular CTLA-4 level in helper T-cells (P = 0.0192), and PD-L1 presenting monocytes (P = 0.0452) than HLC. These levels approached those of HLC over time. No significant differences were found between DILI and VH. However, DILI presented higher level of activated helper T-cells and CTLA-4 than NAFLD and lower PD-L1 level than AIH. Our findings suggest that an adaptive immune response is involved in DILI in which activated CD4+ and CD8+ play an important role. Increased expression of negative immune checkpoints is likely the effect of peripheral tolerance regulation.The present study has been supported by grants of Instituto de Salud Carlos III cofounded by Fondo Europeo de Desarrollo Regional – FEDER (contract numbers: PI19/00883, PI16/01748, P18-RT-3364-2020, and PT20/000127). CIBERehd and Plataforma ISCiii Ensayos Clínicos are funded by Instituto de Salud Carlos III. Funding for open access charge: Universidad de Málaga/CBUA. The funding sources had no involvement in the study design; in the collection, analysis, and interpretation of data; in the writing of the report, or in the decision to submit the manuscript for publication