A theoretical estimate for nucleotide sugar demand towards Chinese Hamster Ovary cellular glycosylation

Glycosylation greatly influences the safety and efficacy of many of the highest-selling recombinant therapeutic proteins (rTPs). In order to define optimal cell culture feeding strategies that control rTP glycosylation, it is necessary to know how nucleotide sugars (NSs) are consumed towards host cell and rTP glycosylation. Here, we present a theoretical framework that integrates the reported glycoproteome of CHO cells, the number of N-linked and O-GalNAc glycosylation sites on individual host cell proteins (HCPs), and the carbohydrate content of CHO glycosphingolipids to estimate the demand of NSs towards CHO cell glycosylation. We have identified the most abundant N-linked and O-GalNAc CHO glycoproteins, obtained the weighted frequency of N-linked and O-GalNAc glycosites across the CHO cell proteome, and have derived stoichiometric coefficients for NS consumption towards CHO cell glycosylation. By combining the obtained stoichiometric coefficients with previously reported data for specific growth and productivity of CHO cells, we observe that the demand of NSs towards glycosylation is significant and, thus, is required to better understand the burden of glycosylation on cellular metabolism. The estimated demand of NSs towards CHO cell glycosylation can be used to rationally design feeding strategies that ensure optimal and consistent rTP glycosylation

Stellar indices and kinematics in Seyfert 1 nuclei

We present spectra of 6 type 1 Seyfert galaxies, 2 Seyfert 2, a starburst galaxy and a compact narrow line radiogalaxy, taken in two spectral ranges centered around the near--IR CaII triplet (CaT) (at ~8600 Angstroms), and the Mgb stellar feature at 5180 Angstroms. We measured the equivalent width (EWs) of these features and the Fe52 and Fe53 spectral indices. We found that the strength of the CaT in type 1 Seyfert galaxies with prominent central point sources, is larger than what would be expected from the observed strength of the blue indices. This could be explained by the presence of red supergiants in the nuclei of Seyfert 1 galaxies. On the other hand, the blue indices of these galaxies could also be diluted by the strong FeII multiplets that can be seen in their spectra. We have also measured the stellar and gas velocity dispersions of the galaxies in the sample. The stellar velocity dispersions were measured using both, the Mgb and CaT stellar features. The velocity dispersion of the gas in the narrow line region (NLR) was measured using the strong emission lines [OIII] 5007, 4959 and [SIII] 9069. We compare the gas and star velocity dispersions and find that both magnitudes are correlated in Seyfert galaxies. Most of the Seyfert 1 we observe have stellar velocity dispersion somehow greater than that of the gas in the NLR.Comment: To appear in MNRAS, 18 pages, 9 figure

A new intermediate mass protostar in the Cepheus A HW2 region

We present the discovery of the first molecular hot core associated with an intermediate mass protostar in the CepA HW2 region. The hot condensation was detected from single dish and interferometric observations of several high excitation rotational lines (from 100 to 880K above the ground state) of SO2 in the ground vibrational state and of HC3N in the vibrationally excited states v7=1 and v7=2. The kinetic temperature derived from both molecules is 160K. The high-angular resolution observations (1.25'' x 0.99'') of the SO2 J=28(7,21)-29(6,24) line (488K above the ground state) show that the hot gas is concentrated in a compact condensation with a size of 0.6''(430AU), located 0.4'' (300AU) east from the radio-jet HW2. The total SO2 column density in the hot condensation is 10E18cm-2, with a H2 column density ranging from 10E23 to 6 x 10E24cm-2. The H2 density and the SO2 fractional abundance must be larger than 10E7cm-3 and 2 x 10E-7 respectively. The most likely alternatives for the nature of the hot and very dense condensation are discussed. From the large column densities of hot gas, the detection of the HC3N vibrationally excited lines and the large SO2 abundance, we favor the interpretation of a hot core heated by an intermediate mass protostar of 10E3 Lo. This indicates that the CepA HW2 region contains a cluster of very young stars

Determinants of Meats Purchase Behavior by Ethnic Groups

Farmers and food companies need to assess their production and marketing strategies for nurturing business opportunities that will arise from the simultaneous increase in population and income of Hispanics in the United States. Previous studies on demand for meat products have not received much attention on the determinants of meats purchase behavior by Hispanics in relation to other ethnic groups. This study investigates the impact of ethnicity and the determinants of meats purchase behavior in the U.S. by using single Probit equations. The analyzed data comes from the U.S. Consumer Expenditure Survey. The results indicate that ethnicity plays an important role in the purchase behavior of meat products, as well as household size and income.consumer behavior, marketing, food demand, Hispanic food, hispanic food market, Latin foods, comida latina, alimentos, ethnic foods, Agribusiness, Consumer/Household Economics, Demand and Price Analysis, Food Consumption/Nutrition/Food Safety, Industrial Organization, Marketing, D01, D03, D12, L14, L66, M31, M37,