Simultaneous Detection of Chlamydia Trachomatis, Neisseria Gonorrhoeae, Ureaplasma Urealyticum by Multiplex PCR-Running

Chlamydia trachomatis (CT), Ureaplasma urealyticum (UU) and Neisseria gonorrhoeae (NG) are the most common pathogens of sexually transmitted infections (STIs), frequently founded in urogenital infections, and showed a criminal role in increasing the risk of potential adverse outcomes. In this study a multiplex PCR assay for the simultaneous detection and accurate identification of 3 clinically relevant pathogens of STIs, i.e., CT, NG and UU in a single tube was developed and evaluated. The limits of detection for the multiplex PCR assay were ~10 copies of DNAs per reaction. This assay has comparable clinical sensitivity to the conventional monoplex real-time PCR assay and considerable potential to be routine molecular diagnostic tool for simultaneous identification of STIs at relatively low cost due to multiplexing

Comparison between Different TomoSAR Imaging Models for Airborne Platform Flying at Low Altitude

The classical planar-wavefront-based TomoSAR imaging model suffers from the problem that the effective integration interval is not enough to cover the target distribution region in the low-altitude airborne case. It will lead to a deterioration of the performance of tomogram reconstruction and inaccuracy of estimated scatterers. This paper reviews the exact and approximate forms of the aforementioned inaccurate model based on planar wavefront and points out the problem with the conventional model. To solve this problem, we propose spherical wavefront models with the exact form or an approximate form of the slant range formula. The estimated variable for the scatterer’s location is converted from elevation to off-nadir angle, and the effective integration interval has been extended. In addition, we explore relationships between the exact form of the conventional model and the exact form of the proposed model, and the relationship between the approximate form of the conventional model and the approximate form of the proposed model. This provides a basis for modifying the inversion algorithm that is designed based on the conventional model to adapt to the low-altitude airborne case. Eventually, through experiments based on simulated data and measured data, the imprecise reconstructions obtained with the conventional model are demonstrated, and the correctness of spherical wavefront models and the effectiveness of transformation between models are proved

A Novel Filtering Method of 3D Reconstruction Point Cloud from Tomographic SAR

With the development of airborne synthetic aperture radar (SAR) technology, the 3D SAR point cloud reconstruction has emerged as a crucial development trend in the current SAR community. However, due to measurement errors, environmental interference, radar decoherence, and other noises associated with the SAR system, the reconstructed tomogram is often deteriorated by numerous noisy scatterers. As a result, it becomes challenging to obtain high-quality 3D point clouds of the observed object, making it difficult to further process the point cloud and realize target identification. To address these issues, we propose a K nearest neighbor comprehensive weighted filtering algorithm. The filtered point cloud is evaluated quantitatively using three-dimensional entropy. In this study, we adopted various filtering methods for simulated data, P-band data of Genhe, and Ku-band data of Yuncheng to refine the tomogram and compare their performances. Both qualitative and quantitative analyses demonstrate the superiority of the filtering algorithm proposed in this paper

Gene Expression Analysis of Human Papillomavirus-Associated Colorectal Carcinoma

Purpose. Human papillomavirus (HPV) antigens had been found in colorectal cancer (CRC) tissue, but little evidence demonstrates the association of HPV with oncogene mutations in CRC. We aim to elucidate the mutated genes that link HPV infection and CRC carcinogenesis. Methods. Cancerous and adjacent noncancerous tissues were obtained from CRC patients. HPV antigen was measured by using the immunohistochemical (IHC) technique. The differentially expressed genes (DEGs) in HPV-positive and HPV-negative tumor tissues were measured by using TaqMan Array Plates. The target genes were validated with the qPCR method. Results. 15 (31.9%) cases of CRC patients were observed to be HPV positive, in which HPV antigen was expressed in most tumor tissues rather than in adjacent noncancerous tissues. With TaqMan Array Plates analyses, we found that 39 differentially expressed genes (DEGs) were upregulated, while 17 DEGs were downregulated in HPV-positive CRC tissues compared with HPV-negative tissues. Four DEGs (MMP-7, MYC, WNT-5A, and AXIN2) were upregulated in tumor vs. normal tissues, or adenoma vs. normal tissue in TCGA, which was overlapped with our data. In the confirmation test, MMP-7, MYC, WNT-5A, and AXIN2 were upregulated in cancerous tissue compared with adjacent noncancerous tissue. MYC, WNT-5A, and AXIN2 were shown to be upregulated in HPV-positive CRC tissues when compared to HPV-negative tissues. Conclusion. HPV-encoding genome may integrate into the tumor genomes that involved in multiple signaling pathways. Further genomic and proteomic investigation is necessary for obtaining a more comprehensive knowledge of signaling pathways associated with the CRC carcinogenesis

Database Resources of the National Genomics Data Center, China National Center for Bioinformation in 2022

The National Genomics Data Center (NGDC), part of the China National Center for Bioinformation (CNCB), provides a family of database resources to support global research in both academia and industry. With the explosively accumulated multi-omics data at ever-faster rates, CNCB-NGDC is constantly scaling up and updating its core database resources through big data archive, curation, integration and analysis. In the past year, efforts have been made to synthesize the growing data and knowledge, particularly in single-cell omics and precision medicine research, and a series of resources have been newly developed, updated and enhanced. Moreover, CNCB-NGDC has continued to daily update SARS-CoV-2 genome sequences, variants, haplotypes and literature. Particularly, OpenLB, an open library of bioscience, has been established by providing easy and open access to a substantial number of abstract texts from PubMed, bioRxiv and medRxiv. In addition, Database Commons is significantly updated by cataloguing a full list of global databases, and BLAST tools are newly deployed to provide online sequence search services